Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,030,732 A→G 5.1% intergenic (‑64/+27) tusE ← / ← yccA mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase/Modulator of FtsH protease, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,030,7320AG 5.1% 428.6 / 7.7 135intergenic (‑64/+27)tusE/yccAmnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase/Modulator of FtsH protease, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (64/64);  new base G (5/2);  total (69/66)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

Only 100 of 136 total aligned reads displayed.

CAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAATGATGTTGCTG  >  NC_000913/1030584‑1030879
                                                                                                                                                    |                                                                                                                                                   
cagccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGa                                                                                                                                                     <  1:412655/149‑1 (MQ=255)
   ccagtGTCTAACTACACTGGCTGCTTTCTTTTTGATAGCCTTCGGTATCCGTTTCTATCTCATTACCTTAGAATATCATCAAAATTATTACAACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGc                                                                                                                                                  <  2:310142/149‑1 (MQ=255)
   ccagcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTAACCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGc                                                                                                                                                  >  1:937398/1‑149 (MQ=255)
     agcGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTa                                                                                                                                                >  2:1304499/1‑149 (MQ=255)
        ggCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTTTATGCGGGGCTTTgt                                                                                                                                             <  1:1464462/149‑2 (MQ=255)
        ggCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCTCATAAAGCGAGGCTATGa                                                                                                                                             <  2:264919/149‑1 (MQ=255)
             aCTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCTCGCTTTATGCGGGGCTTTgtttttt                                                                                                                                        <  2:1207730/149‑7 (MQ=255)
              cTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTACTCAGACTGGCGGCAGTGTAACAAAGAACAAAGCCCCGCATAAAGCGAGGCTATGAAAgtgt                                                                                                                                       <  1:1159606/149‑1 (MQ=255)
               tCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGtt                                                                                                                                      >  1:343202/1‑149 (MQ=255)
                 cACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAg                                                                                                                                    <  1:952369/149‑1 (MQ=255)
                 cACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAg                                                                                                                                    >  1:1531298/1‑149 (MQ=255)
                      gctgctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGt                                                                                                                               <  2:937398/149‑1 (MQ=255)
                       ctgctTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTg                                                                                                                              <  1:1701/149‑1 (MQ=255)
                          ctttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTgaga                                                                                                                           <  1:121423/149‑1 (MQ=255)
                           tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                                          <  1:763054/149‑1 (MQ=255)
                           tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                                          >  2:1484781/1‑149 (MQ=255)
                           tttctttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGACAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAt                                                                                                                          >  1:1224895/1‑149 (MQ=255)
                               tttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAAt                                                                                                                      >  2:1463100/1‑149 (MQ=255)
                               tttGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAAt                                                                                                                      <  1:1182248/149‑1 (MQ=255)
                                  gagaTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATcgc                                                                                                                   >  2:1368993/1‑149 (MQ=255)
                                     attgCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACAACCTTAAACAGACTGGCGGCATTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGAATAATCGCGGc                                                                                                                >  2:634706/4‑149 (MQ=255)
                                     aTAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGc                                                                                                                <  1:1143368/149‑1 (MQ=255)
                                      tAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCt                                                                                                               >  2:1434972/1‑149 (MQ=255)
                                         ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                            >  2:139382/1‑149 (MQ=255)
                                         ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                            >  2:1312279/1‑149 (MQ=255)
                                         ccTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                            >  1:1107635/1‑149 (MQ=255)
                                         ccTTCGGTATCCGTTTCTACCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGc                                                                                                            >  2:927255/1‑149 (MQ=255)
                                           ttCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGa                                                                                                          >  1:377591/1‑149 (MQ=255)
                                            tCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGaa                                                                                                         <  2:1142336/149‑1 (MQ=255)
                                             cGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTACTCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAg                                                                                                        <  1:280929/149‑1 (MQ=255)
                                              ggTATCAGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTTGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGc                                                                                                       <  2:842983/149‑1 (MQ=255)
                                               gTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGcc                                                                                                      >  1:746709/1‑149 (MQ=255)
                                                 aTCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCa                                                                                                    <  1:1437955/149‑1 (MQ=255)
                                                    cGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGaa                                                                                                 >  1:1438923/1‑149 (MQ=255)
                                                    cGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGaa                                                                                                 <  2:638021/149‑1 (MQ=255)
                                                        tCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCt                                                                                             <  1:1368993/149‑1 (MQ=255)
                                                           aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                                          >  2:727147/1‑149 (MQ=255)
                                                           aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                                          >  1:937899/1‑149 (MQ=255)
                                                           aTCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTcag                                                                                          <  1:1298104/149‑1 (MQ=255)
                                                               ctTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGATAGTGTGAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGCATGCTCAGCAgg                                                                                      >  1:91999/1‑149 (MQ=255)
                                                                tttACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGc                                                                                     >  2:524018/1‑149 (MQ=255)
                                                                 ttACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCt                                                                                    <  1:1273162/149‑1 (MQ=255)
                                                                   aCCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGa                                                                                  <  1:1353728/149‑1 (MQ=255)
                                                                    ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                                 >  2:1347028/1‑149 (MQ=255)
                                                                    ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                                 >  2:109961/1‑149 (MQ=255)
                                                                    ccTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGAc                                                                                 >  2:1067233/1‑149 (MQ=255)
                                                                     cTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACg                                                                                <  2:1424169/149‑1 (MQ=255)
                                                                      ttCGAAGATCAGCATAATTATTACTACATTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCACTCATAAAGCGAGTCTATGAAAGTGTTAGAGGGTGAGATTAATCGCGGCTAGCTAAGCCCAGAATGCTCAGCAGGCTGACGa                                                                               >  1:124956/1‑149 (MQ=255)
                                                                          aaGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAt                                                                           >  1:713925/1‑149 (MQ=255)
                                                                           aGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                                          <  2:746709/149‑1 (MQ=255)
                                                                           aGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                                          >  1:192261/1‑149 (MQ=255)
                                                                           aGATAAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtg                                                                          <  2:4790/149‑1 (MQ=255)
                                                                            gATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtgt                                                                         >  1:868819/1‑149 (MQ=255)
                                                                              tCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGAtgttg                                                                       <  1:1472581/149‑1 (MQ=255)
                                                                                aGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTa                                                                     >  1:754127/1‑149 (MQ=255)
                                                                                 gCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTAc                                                                    <  1:901630/149‑1 (MQ=255)
                                                                                 gCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTAc                                                                    >  1:1333798/1‑149 (MQ=255)
                                                                                    tAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGc                                                                 >  2:603686/1‑149 (MQ=255)
                                                                                     aaTTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCg                                                                >  1:1536122/1‑149 (MQ=255)
                                                                                      attattACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCACGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGAGAGATTAATCGCGGCTAGCGAAGCCCAGAAAGCTCAGCAGGATCACGCAGATGTTGTTCAGCGa                                                               >  1:328978/1‑149 (MQ=255)
                                                                                       ttattaCTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAACCCCAGAATGCACAGCAGGCAGACGAAGATGTTGTACAGTGaa                                                              >  2:1068722/1‑149 (MQ=255)
                                                                                        tattaCTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGaaa                                                             <  2:1369369/149‑1 (MQ=255)
                                                                                             ctacCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATAc                                                        >  1:453638/1‑149 (MQ=255)
                                                                                              tacCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACa                                                       >  1:84855/1‑149 (MQ=255)
                                                                                               acCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCTAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAg                                                      <  1:1240820/149‑1 (MQ=255)
                                                                                                ccTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAgg                                                     <  2:868819/149‑1 (MQ=255)
                                                                                                ccTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAgg                                                     >  1:210535/1‑149 (MQ=255)
                                                                                                        aGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGACACATACAGGCTAACCGt                                             >  1:14770/1‑149 (MQ=255)
                                                                                                         gACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTg                                            >  1:1078061/1‑149 (MQ=255)
                                                                                                         gACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTg                                            <  1:1119817/149‑1 (MQ=255)
                                                                                                            tGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCa                                         >  1:1110488/1‑149 (MQ=255)
                                                                                                            tGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCa                                         <  2:1187591/149‑1 (MQ=255)
                                                                                                             ggcggcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCAc                                        <  2:192261/149‑1 (MQ=255)
                                                                                                             ggcggcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCAc                                        <  1:1304499/149‑1 (MQ=255)
                                                                                                              gcggcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACg                                       >  2:1077988/1‑149 (MQ=255)
                                                                                                                 gcAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCTGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGTCACGAat                                    <  1:414753/149‑1 (MQ=255)
                                                                                                                   aGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAatat                                  <  1:524018/149‑1 (MQ=255)
                                                                                                                    ttgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAAtata                                 <  1:765389/148‑1 (MQ=255)
                                                                                                                    gtgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGACCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAAtata                                 <  1:139382/149‑1 (MQ=255)
                                                                                                                     tgtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCAGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAg                                <  2:377591/149‑1 (MQ=255)
                                                                                                                      gtAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGt                               <  2:1571106/149‑1 (MQ=255)
                                                                                                                       tAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGtt                              >  2:1212184/1‑149 (MQ=255)
                                                                                                                        aaCAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTc                             >  2:1477428/1‑149 (MQ=255)
                                                                                                                          cAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGCCGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGt                           >  2:814116/1‑149 (MQ=255)
                                                                                                                          aaaaaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGt                           <  1:1312279/148‑1 (MQ=255)
                                                                                                                           aaaaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGtc                          >  1:460887/1‑149 (MQ=255)
                                                                                                                           aaaaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGtc                          >  2:506726/1‑149 (MQ=255)
                                                                                                                             aaaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGtctc                        <  1:902819/149‑1 (MQ=255)
                                                                                                                              aaaCAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCa                       >  2:833334/1‑149 (MQ=255)
                                                                                                                                  ataGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGc                   >  1:1342150/3‑149 (MQ=255)
                                                                                                                                  ataGCCTCGCTTTATGCGGGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGc                   >  2:479052/3‑149 (MQ=255)
                                                                                                                                     gCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCat                <  2:46852/149‑1 (MQ=255)
                                                                                                                                      ccccGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCatg               >  1:1191776/1‑149 (MQ=255)
                                                                                                                                        ccGCATAAAGCGAGGCTATGAAAGTGTTAGCTGGTGAGATTAATCGCGGCTAGCGATGCCCAGAATGCTCAGCAGGCAGCCGAAGATGTTGTACAGCGAAACATACAGGCTAGCCGTGGCACGAATATAGTTCGTCTCACCGCCatgaa             >  1:806025/1‑149 (MQ=255)
                                                                                                                                            aTAAAGCGAGGCTATGAAAGAGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATTCTCAGCAGGCTGACGAAGATGTTGTACAGTGAAACATACAGGGTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAatgat         <  1:1068722/149‑1 (MQ=255)
                                                                                                                                               aaGCGAGGCTATGAAAGTGTTAGCGGGGGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGGGGCACGAATATAGTTCGTCGCGCCGCCatgaatgagggg      >  1:288213/1‑145 (MQ=255)
                                                                                                                                                aGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAATGATGTTg     <  1:389684/149‑1 (MQ=255)
                                                                                                                                                  cGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGTTCAGCTGGCTGACGAAGATGTTGTACAGCGAAACATTCTGGCTAACCGTGGCTCGAATATAGTTCGTCTCACCGCCATGAATGATGTTGCt   <  1:319124/149‑1 (MQ=255)
                                                                                                                                                   gAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAATGATGTTGCTg  <  2:378103/149‑1 (MQ=255)
                                                                                                                                                   gAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAATGATGTTGCTg  <  1:1077988/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGCCAGCGGCTCACTCCACTGGCTGCTTTCTTTGAGATAGCCTTCGGTATCCGTTTCTATCTCTTTACCTTCGAAGATCAGCATAATTATTACTACCTTAATCAGACTGGCGGCAGTGTAACAAAAAACAAAGCCCCGCATAAAGCGAGGCTATGAAAGTGTTAGCGGGTGAGATTAATCGCGGCTAGCGAAGCCCAGAATGCTCAGCAGGCTGACGAAGATGTTGTACAGCGAAACATACAGGCTAACCGTGGCACGAATATAGTTCGTCTCACCGCCATGAATGATGTTGCTG  >  NC_000913/1030584‑1030879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: