Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,101,555 C→A 6.2% A44S (GCG→TCG) ‡ csgG ← curli production assembly/transport outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,101,5550CA 6.2% 286.0 / 6.2 92A44S (GCG→TCG) ‡csgGcurli production assembly/transport outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (38/48);  new base A (2/4);  total (40/52)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

AACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTT  >  NC_000913/1101407‑1101697
                                                                                                                                                    |                                                                                                                                              
aaCCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGgcgc                                                                                                                                                >  2:288962/1‑149 (MQ=255)
 aCCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATTCCGAAACAAAGATTTTACCCGTCGGCGCt                                                                                                                                               >  2:8437/1‑149 (MQ=255)
 aCCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCt                                                                                                                                               >  2:332031/1‑149 (MQ=255)
 aCCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCt                                                                                                                                               >  2:322013/1‑149 (MQ=255)
  ccAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTg                                                                                                                                              <  2:657385/149‑1 (MQ=255)
   cAGCGAGAATCTTTCAGTGACGTGACCAGCAATCCCGTGGAGCTTTGCGGAACAGCAGTGGAGAAGTTACTAGCCGGGTAGGGTTAAAATAGCCCGCTTTCGTCCTGAATGTTGTATACCGAAACAAATATATTACCCGTCGGCGCTgg                                                                                                                                             >  1:1170598/1‑149 (MQ=255)
       gagaATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGa                                                                                                                                         <  1:1001644/149‑1 (MQ=255)
          aaTCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATgg                                                                                                                                      <  1:1058333/149‑1 (MQ=255)
            tCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTATATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACACGTCGGCGCTGGCAGATGGGt                                                                                                                                    <  2:1142691/149‑1 (MQ=255)
               ttCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGAT‑GGTCaaa                                                                                                                                <  2:854786/149‑1 (MQ=255)
                    tGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCtt                                                                                                                            <  2:1318344/149‑1 (MQ=37)
                     gCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCttt                                                                                                                           <  1:530566/149‑1 (MQ=37)
                      ccGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTg                                                                                                                          >  1:796738/1‑149 (MQ=255)
                      ccGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTGTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTg                                                                                                                          >  1:791461/1‑149 (MQ=255)
                      ccGTGACCAGCATTGCCGTGGCGCTTTGAGTATCAGAATTTGAGAAGTTACTTGCCGTGTAGGGTTTAAATTGCCCGTTTTCGTACTGAATGTTGTATACCTAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTg                                                                                                                          <  2:1133068/149‑1 (MQ=16)
                         tGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAg                                                                                                                       >  1:888215/1‑149 (MQ=255)
                           aCCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGct                                                                                                                     >  2:1310940/1‑149 (MQ=255)
                            ccAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGtttcgg                                                                                                                    <  2:316443/149‑6 (MQ=25)
                             cAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGctct                                                                                                                   >  2:238807/1‑149 (MQ=255)
                             cAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGctct                                                                                                                   >  2:825497/1‑149 (MQ=255)
                             cAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCACGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGctct                                                                                                                   >  1:1440911/1‑149 (MQ=255)
                               gCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGa                                                                                                                 <  2:701210/149‑1 (MQ=255)
                                     ccGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGa                                                                                                           >  2:573542/1‑149 (MQ=255)
                                      cGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAg                                                                                                          <  1:1494849/149‑1 (MQ=255)
                                        tGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGTGTTAAAATTGCCCGGATTCGTCCTGAATGTTGTATACCGAAACAAAGAATTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCAGAGCACGAGGc                                                                                                        >  1:1436090/1‑149 (MQ=255)
                                           cgcTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCAtt                                                                                                     <  2:536470/149‑1 (MQ=255)
                                           cgcTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGGTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCAtt                                                                                                     <  2:1289931/149‑1 (MQ=255)
                                            gcTTTGCGGAACAGCAGTGGAGAAGTTACTTGTCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTa                                                                                                    >  1:575127/1‑149 (MQ=255)
                                              tttGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAAt                                                                                                  <  1:927550/149‑1 (MQ=255)
                                              tttGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAAt                                                                                                  >  1:440670/1‑149 (MQ=255)
                                                tGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGt                                                                                                <  2:443652/149‑1 (MQ=255)
                                                 gCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTc                                                                                               <  2:1391983/149‑1 (MQ=255)
                                                  cGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAg                                                                                                          >  2:1157460/1‑137 (MQ=255)
                                                  cGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAg                                                                                                          <  1:1157460/137‑1 (MQ=255)
                                                  cGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTATAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGTCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGAATTAATTTCg                                                                                              >  1:966985/1‑149 (MQ=255)
                                                                 taaGGTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACATAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGATCACGAGGCATTAATGTCGGTCTGGCGGcttctt                                                                               <  1:967060/148‑1 (MQ=255)
                                                                 gaAGTTACATGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttctt                                                                               >  2:1532351/1‑149 (MQ=255)
                                                                  aaGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCttt                                                                              <  2:1312584/149‑1 (MQ=255)
                                                                  aaGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCttt                                                                              >  2:738446/1‑149 (MQ=255)
                                                                   aGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTa                                                                             <  1:665018/149‑1 (MQ=255)
                                                                    gTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAg                                                                            >  1:1242716/1‑149 (MQ=255)
                                                                     ttACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTTAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAgg                                                                           >  1:1110498/1‑149 (MQ=255)
                                                                        cTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcgg                                                                        <  1:899948/149‑1 (MQ=255)
                                                                         ttGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggg                                                                       >  2:50406/1‑149 (MQ=255)
                                                                              gtgtattgTTTAAATTTCCCGGTTTCGTCATGAATGTTGTACACCTAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGCGNNATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGt                                                                  <  2:705264/142‑1 (MQ=255)
                                                                                gTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTa                                                                >  2:1275379/1‑149 (MQ=255)
                                                                                 tAGTGTGTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTAACCGTCGGAGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                               <  1:295789/149‑1 (MQ=255)
                                                                                 tAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                               <  1:764512/149‑1 (MQ=255)
                                                                                 tAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                               <  1:1519687/149‑1 (MQ=255)
                                                                                 tAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                               <  1:415099/149‑1 (MQ=255)
                                                                                 tAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                               <  1:573542/149‑1 (MQ=255)
                                                                                 tAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                               <  1:672084/149‑1 (MQ=255)
                                                                                 tAGGGGTTAAATTGCCCGGTGTCGTCCTGACTGTTGTATACCGAAACAAAGATTTTAACCGTCGGCGCTGGCAGATGTGTCAAATCTTTGTAGCTCTGAGCAAGAGTCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                               <  1:262551/149‑1 (MQ=255)
                                                                                     gTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCAt                                                           >  2:709979/1‑149 (MQ=255)
                                                                                     gTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCAt                                                           <  2:888215/149‑1 (MQ=255)
                                                                                     gTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCAt                                                           >  2:293570/1‑149 (MQ=255)
                                                                                      tttAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATc                                                          >  1:197521/1‑149 (MQ=255)
                                                                                       ttAAATTGCCCGGTTTCGTCCAGAATGTTGTATTCCGAAACAAAGATTTTACCAGTCGGCTCTTGCAGATGGGTCAAATCTTAGTAGCTCTGAGCACGAGGCATTAATGTAGGTCTGGCGGCTTCTTTAGGCGGGGCTGTTTAGAATcc                                                         >  1:1390330/1‑149 (MQ=255)
                                                                                         aaaTTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGc                                                       <  2:1357662/149‑1 (MQ=255)
                                                                                          aaTTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCCTCCGCt                                                      >  1:1075117/1‑149 (MQ=255)
                                                                                                ccGGTTTCGCCCTGAATGTTGTATACCGAAACAAAGATTATACCCGGCGGCGCTGGCAGATGGGTCTAATCTTTGTAGATCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTaa                                                <  1:300232/149‑1 (MQ=255)
                                                                                                       cGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACg                                         <  2:1045149/149‑1 (MQ=255)
                                                                                                        gTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACTAGGCATTAATGTCGGTCTGGCGCCTTCTTTAGGCGGGCCGGTTAAGCATCCGCTCAGTAACATGACgg                                        >  2:1230366/1‑149 (MQ=255)
                                                                                                        gTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACgg                                        >  1:1335333/1‑149 (MQ=255)
                                                                                                           cTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCaa                                     >  1:38479/1‑149 (MQ=255)
                                                                                                            tGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAAc                                    >  1:1007928/1‑149 (MQ=255)
                                                                                                            tGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAAc                                    >  2:249954/1‑149 (MQ=255)
                                                                                                            tGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAAc                                    <  2:791461/149‑1 (MQ=255)
                                                                                                            tGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAAc                                    <  2:796738/149‑1 (MQ=255)
                                                                                                               aTGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCCCGAGGCAGTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGCCGGCAACCaa                                 >  2:190841/1‑149 (MQ=255)
                                                                                                                     tataCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                               >  1:138511/1‑113 (MQ=255)
                                                                                                                     tataCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTaa                                                               <  2:138511/113‑1 (MQ=255)
                                                                                                                      ataCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAAt                          <  1:322013/149‑1 (MQ=255)
                                                                                                                        aCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAATTCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTTAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAATTaa                        <  2:1451993/149‑1 (MQ=255)
                                                                                                                          cGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATTTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                      <  1:238807/149‑1 (MQ=255)
                                                                                                                             taCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCt                   >  2:548515/2‑149 (MQ=37)
                                                                                                                              aCAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTg                  <  2:1569502/149‑1 (MQ=255)
                                                                                                                              aCAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTg                  <  2:1563349/149‑1 (MQ=255)
                                                                                                                                aaaGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTAGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                >  1:46539/1‑149 (MQ=255)
                                                                                                                                aaaGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                >  1:525367/1‑149 (MQ=37)
                                                                                                                                 aaGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCAt               >  1:1221277/1‑149 (MQ=255)
                                                                                                                                        tACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGattatt        <  1:577841/149‑1 (MQ=255)
                                                                                                                                         aCCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTAttt       <  1:825497/149‑1 (MQ=255)
                                                                                                                                         aCCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTAttt       <  2:147391/149‑1 (MQ=255)
                                                                                                                                          accttCGGCGCTTGCAGATGGGTAAAATCTTTGTAGCTCTGAGCACGATGCATTAATGTCGGTATGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCATCCATAAGAAATAAGCGCTGCATGATTAtttt      <  2:1390330/145‑1 (MQ=255)
                                                                                                                                           ccGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTc     <  2:923131/149‑1 (MQ=255)
                                                                                                                                           ccGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTc     <  2:38479/149‑1 (MQ=255)
                                                                                                                                           ccGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTc     <  2:1440911/149‑1 (MQ=255)
                                                                                                                                           ccGTCGGCGCGGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTc     <  1:288962/149‑1 (MQ=255)
                                                                                                                                            cGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTcc    >  1:1395016/1‑149 (MQ=255)
                                                                                                                                             gTCTGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGGCACCAAAAGAAATAAGCGCTGCATGATTATTTTCCt   <  1:25947/149‑1 (MQ=255)
                                                                                                                                             gTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCGCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCt   <  2:1007928/149‑1 (MQ=255)
                                                                                                                                              tCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAAAGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCtt  <  1:332031/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
AACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTT  >  NC_000913/1101407‑1101697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑