Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,134,669 T→A 6.9% intergenic (+112/‑60) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,134,6690TA 6.9% 307.2 / 9.1 101intergenic (+112/‑60)flgF/flgGflagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base T (53/41);  new base A (4/3);  total (57/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 104 total aligned reads displayed.

GGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAG  >  NC_000913/1134525‑1134817
                                                                                                                                                |                                                                                                                                                    
ggCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATTGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                                  <  2:48438/149‑1 (MQ=255)
ggCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                                  <  1:1342154/149‑1 (MQ=255)
 gCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                                 >  2:228078/1‑149 (MQ=255)
   cGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                               <  2:1059532/149‑1 (MQ=255)
       ccaaccaaCTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                           >  2:804622/1‑149 (MQ=255)
          accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                        <  1:871884/149‑1 (MQ=255)
            caacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                                                                                                                                      <  2:23927/149‑1 (MQ=255)
              acTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaaga                                                                                                                                    >  1:1243103/1‑149 (MQ=255)
                tgctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcctacaa                                                                                                                                  <  1:383831/149‑7 (MQ=38)
                tgctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaagaag                                                                                                                                  <  2:1285754/149‑1 (MQ=255)
                  ctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAc                                                                                                                                >  2:1341537/1‑149 (MQ=255)
                  ctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAc                                                                                                                                >  2:1599830/1‑149 (MQ=255)
                  ctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAc                                                                                                                                <  2:214234/149‑1 (MQ=255)
                  ctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTAAGGGGGCAATCGCAATTAATCGCTTATGCGAGCACTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAATAAGAc                                                                                                                                >  2:106929/1‑149 (MQ=39)
                   tgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACa                                                                                                                               >  2:663883/1‑149 (MQ=255)
                   tgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACa                                                                                                                               >  1:831949/1‑149 (MQ=255)
                   tgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACa                                                                                                                               >  2:1278682/1‑149 (MQ=255)
                    gTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGAAGCGCAAGTTTATCGGGTCTATGGGCGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAAGAAGCCat                                                                                                                              >  2:252319/1‑149 (MQ=255)
                     tCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACata                                                                                                                             >  1:1009609/1‑149 (MQ=255)
                      agATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACatat                                                                                                                            <  1:446030/148‑1 (MQ=255)
                       gATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATg                                                                                                                           >  2:542427/1‑149 (MQ=255)
                          gAGTTAATTGATAGGATACATGACAAGTATAAGTTGCCCGATGCGCTAGTTAATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCAc                                                                                                                        <  1:1364912/149‑1 (MQ=255)
                          gAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCAc                                                                                                                        <  2:1163468/149‑1 (MQ=255)
                            gTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACtt                                                                                                                      >  2:1127572/1‑149 (MQ=255)
                               aaTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGt                                                                                                                   >  2:1254908/1‑149 (MQ=255)
                               aaTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGt                                                                                                                   <  2:133791/149‑1 (MQ=255)
                                aTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTc                                                                                                                  >  1:970619/1‑149 (MQ=255)
                                aTTGAAAGGATACATGACAAGTATAAGTAACCAGATGCGCAAGTTTATCTGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGACGGAGAAGGCGTTTAAGACGCATACTTCAAGAAGACATATGCCCTATGta                                                                                                                  >  1:258849/1‑148 (MQ=255)
                                     aaGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTaa                                                                                                             <  1:1360742/149‑1 (MQ=255)
                                       ggATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATc                                                                                                           <  1:709440/149‑1 (MQ=255)
                                       ggATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATc                                                                                                           <  2:863140/149‑1 (MQ=255)
                                        gATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATcc                                                                                                          >  2:409721/1‑149 (MQ=255)
                                         aTACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCa                                                                                                         >  2:887068/1‑149 (MQ=255)
                                          tACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCAc                                                                                                        <  1:215768/149‑1 (MQ=255)
                                           aCATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACt                                                                                                       >  1:1414999/1‑149 (MQ=255)
                                            cATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTa                                                                                                      <  1:887068/149‑1 (MQ=255)
                                              tGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCATTCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACa                                                                                                    >  1:1469233/1‑149 (MQ=255)
                                              tGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACa                                                                                                    <  1:926804/149‑1 (MQ=255)
                                              tGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGAACTTTGTCACTAATCCACTACa                                                                                                    <  1:808731/149‑1 (MQ=255)
                                               gACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAg                                                                                                   <  2:1225368/149‑1 (MQ=255)
                                                aCAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAgg                                                                                                  >  2:1240328/1‑149 (MQ=255)
                                                 cAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGa                                                                                                 >  1:32238/1‑149 (MQ=255)
                                                   aGTATAAGTTGCCCGATGCGCAAGTTTATAGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATACGGCGTTTACGCCGCATCCGTCAAGAAGACATATGCAGGTTTTCACTAATCCACTACAGGACa                                                                                               >  1:112615/1‑149 (MQ=255)
                                                    gTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAt                                                                                              >  1:1311244/1‑149 (MQ=255)
                                                     tataAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAtt                                                                                             >  2:897798/1‑149 (MQ=255)
                                                      ataAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAttt                                                                                            >  2:1488604/1‑149 (MQ=255)
                                                       taAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCACAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAtttt                                                                                           >  1:1339975/1‑149 (MQ=255)
                                                         atcttcccGAATCGCAATTTTATCCGGTCTATGGGGTCTATCGCAAATTAACTATTTTGGGATCACTTCTAGGCCGGATAAGGCGTTTACGCCTCATCCGTCAAGAAGACATATTCACTTTGTCACTAATCCACTACAGGACATTTTAt                                                                                         <  1:234040/144‑1 (MQ=255)
                                                         aGTTGCCCTATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTAt                                                                                         <  1:1384836/149‑1 (MQ=255)
                                                         aGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTAt                                                                                         <  1:690831/149‑1 (MQ=255)
                                                         aGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTAt                                                                                         <  2:1271257/149‑1 (MQ=255)
                                                            tGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGAt                                                                                      <  2:1322172/149‑1 (MQ=255)
                                                              cccGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCa                                                                                    <  2:1488851/149‑1 (MQ=255)
                                                              cccGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCa                                                                                    <  2:1243103/149‑1 (MQ=255)
                                                                 gATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGtt                                                                                 >  1:925121/1‑149 (MQ=255)
                                                                   tGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCa                                                                               <  1:1092280/149‑1 (MQ=255)
                                                                     cgcAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCAtt                                                                             <  1:20482/149‑1 (MQ=255)
                                                                      gcAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTa                                                                            <  1:686765/149‑1 (MQ=255)
                                                                       cAAGTTTATCGGGTCTATGGGGGCACTCGCAATTTATAGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCAATACAGGACATTTTATGATCAGTTCATTAt                                                                           >  1:969480/1‑149 (MQ=255)
                                                                       cAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTAt                                                                           <  2:1561213/149‑1 (MQ=255)
                                                                           tttATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAATGCGTTTACGCCGCATCCGGCTAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGAt                                                                       >  1:210199/1‑149 (MQ=255)
                                                                            ttATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATc                                                                      >  2:825762/1‑149 (MQ=255)
                                                                             tATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCg                                                                     >  2:419793/1‑149 (MQ=255)
                                                                               tCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGcc                                                                   >  2:1049945/1‑149 (MQ=255)
                                                                                  ggTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaaa                                                                >  2:623605/1‑149 (MQ=255)
                                                                                   gTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaaaa                                                               >  1:696428/1‑149 (MQ=255)
                                                                                   gTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaaaa                                                               <  1:663883/149‑1 (MQ=255)
                                                                                           gggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCtt                                                       >  2:766992/1‑149 (MQ=255)
                                                                                           gggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCtt                                                       >  1:1326199/1‑149 (MQ=255)
                                                                                              cAATCGGAATTTATAGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAAGAAGACATTTGCACTTTGTCACTAATCCACTACAGGACATTTTATTATCAGTTCATTATGGATCGCCAATACGGGCCTTGAc                                                    <  2:258849/149‑1 (MQ=39)
                                                                                              cAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGAc                                                    >  2:1137450/1‑149 (MQ=255)
                                                                                              cAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGAc                                                    <  2:1501674/149‑1 (MQ=255)
                                                                                                 tCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGcc                                                 >  1:1314018/1‑149 (MQ=255)
                                                                                                    cAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGACACAACGGGCCTTGACGCCCAg                                              >  1:135804/1‑149 (MQ=255)
                                                                                                        ttATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCaaa                                          <  1:1278682/149‑1 (MQ=255)
                                                                                                             gATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAat                                     <  1:1488604/149‑1 (MQ=255)
                                                                                                              aTTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAata                                    >  1:915097/1‑149 (MQ=255)
                                                                                                                tttGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATg                                  <  1:1430722/149‑1 (MQ=255)
                                                                                                                   gCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGAc                               <  1:804622/149‑1 (MQ=255)
                                                                                                                         aCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCAtt                         >  2:1498916/1‑149 (MQ=255)
                                                                                                                            ttcttgCCGGATGCGGCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGcc                      >  1:747616/6‑149 (MQ=38)
                                                                                                                               ttgCCGGATGCGGCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaac                   >  2:222839/3‑149 (MQ=255)
                                                                                                                               aGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaac                   >  2:5890/1‑149 (MQ=255)
                                                                                                                                ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaaca                  >  2:1295472/1‑149 (MQ=255)
                                                                                                                                 gCCGGATGCGGCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTGTATGATCAGTTCATTATGGATCGCCAAACCGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaacaa                 >  2:450668/1‑149 (MQ=255)
                                                                                                                                  ccGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCaacaac                <  1:1295472/149‑1 (MQ=255)
                                                                                                                                   cGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGTCATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc               >  1:974203/1‑149 (MQ=255)
                                                                                                                                   cGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTTACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc               >  1:1393677/1‑149 (MQ=255)
                                                                                                                                   cGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc               >  1:1195237/1‑149 (MQ=255)
                                                                                                                                     gatgcggCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg             >  2:1514295/6‑149 (MQ=255)
                                                                                                                                     gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg             <  2:1311244/149‑1 (MQ=255)
                                                                                                                                     gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg             <  1:999411/149‑1 (MQ=255)
                                                                                                                                     gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg             >  2:1289461/1‑149 (MQ=255)
                                                                                                                                     gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg             <  1:409721/149‑1 (MQ=255)
                                                                                                                                      atgcggCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTgg            <  2:747616/145‑1 (MQ=255)
                                                                                                                                      aTAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTgg            >  1:1546856/1‑149 (MQ=255)
                                                                                                                                        aaGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCa          >  2:30637/1‑149 (MQ=255)
                                                                                                                                               ttACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCa   <  2:1195237/149‑1 (MQ=255)
                                                                                                                                                tACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAg  <  1:1127572/149‑1 (MQ=255)
                                                                                                                                                tACGCCGCATCCGGCAAGAAGACATATGCACTTTGGCACTAATCCACTACATGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTCGCAAACGTCAg  <  2:112615/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAG  >  NC_000913/1134525‑1134817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: