Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,134,675 G→T 7.9% intergenic (+118/‑54) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,134,6750GT 7.9% 279.8 / 8.5 102intergenic (+118/‑54)flgF/flgGflagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base G (53/40);  new base T (4/5);  total (57/45)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 108 total aligned reads displayed.

CGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACT  >  NC_000913/1134528‑1134821
                                                                                                                                                   |                                                                                                                                                  
cGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                                   <  2:1059532/149‑1 (MQ=255)
    ccaaccaaCTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                               >  2:804622/1‑149 (MQ=255)
       accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                            <  1:871884/149‑1 (MQ=255)
         caacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                                                                                                                                          <  2:23927/149‑1 (MQ=255)
           acTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaaga                                                                                                                                        >  1:1243103/1‑149 (MQ=255)
             tgctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcctacaa                                                                                                                                      <  1:383831/149‑7 (MQ=38)
             tgctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaagaag                                                                                                                                      <  2:1285754/149‑1 (MQ=255)
               ctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAc                                                                                                                                    >  2:1341537/1‑149 (MQ=255)
               ctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAc                                                                                                                                    >  2:1599830/1‑149 (MQ=255)
               ctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAc                                                                                                                                    <  2:214234/149‑1 (MQ=255)
               ctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTAAGGGGGCAATCGCAATTAATCGCTTATGCGAGCACTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAATAAGAc                                                                                                                                    >  2:106929/1‑149 (MQ=39)
                tgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACa                                                                                                                                   >  1:831949/1‑149 (MQ=255)
                tgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACa                                                                                                                                   >  2:663883/1‑149 (MQ=255)
                 gTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGAAGCGCAAGTTTATCGGGTCTATGGGCGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAAGAAGCCat                                                                                                                                  >  2:252319/1‑149 (MQ=255)
                  tCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACata                                                                                                                                 >  1:1009609/1‑149 (MQ=255)
                   agATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACatat                                                                                                                                <  1:446030/148‑1 (MQ=255)
                    gATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATg                                                                                                                               >  2:542427/1‑149 (MQ=255)
                       gAGTTAATTGATAGGATACATGACAAGTATAAGTTGCCCGATGCGCTAGTTAATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCAc                                                                                                                            <  1:1364912/149‑1 (MQ=255)
                       gAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCAc                                                                                                                            <  2:1163468/149‑1 (MQ=255)
                         gTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACtt                                                                                                                          >  2:1127572/1‑149 (MQ=255)
                            aaTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGt                                                                                                                       >  2:1254908/1‑149 (MQ=255)
                            aaTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGt                                                                                                                       <  2:133791/149‑1 (MQ=255)
                             aTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTc                                                                                                                      >  1:970619/1‑149 (MQ=255)
                             aTTGAAAGGATACATGACAAGTATAAGTAACCAGATGCGCAAGTTTATCTGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGACGGAGAAGGCGTTTAAGACGCATACTTCAAGAAGACATATGCCCTATGta                                                                                                                      >  1:258849/1‑148 (MQ=255)
                                    ggATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATc                                                                                                               <  1:709440/149‑1 (MQ=255)
                                    ggATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATc                                                                                                               <  2:863140/149‑1 (MQ=255)
                                     gATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATcc                                                                                                              >  2:409721/1‑149 (MQ=255)
                                      aTACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCa                                                                                                             >  2:887068/1‑149 (MQ=255)
                                       tACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCAc                                                                                                            <  1:215768/149‑1 (MQ=255)
                                        aCATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACt                                                                                                           >  1:1414999/1‑149 (MQ=255)
                                         cATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTa                                                                                                          <  1:887068/149‑1 (MQ=255)
                                          aTGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTAc                                                                                                         >  2:1509111/1‑149 (MQ=255)
                                           tGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCATTCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACa                                                                                                        >  1:1469233/1‑149 (MQ=255)
                                           tGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACa                                                                                                        <  1:926804/149‑1 (MQ=255)
                                           tGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGAACTTTGTCACTAATCCACTACa                                                                                                        <  1:808731/149‑1 (MQ=255)
                                            gACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAg                                                                                                       <  2:1225368/149‑1 (MQ=255)
                                             aCAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAgg                                                                                                      >  2:1240328/1‑149 (MQ=255)
                                              cAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGa                                                                                                     >  1:32238/1‑149 (MQ=255)
                                                aGTATAAGTTGCCCGATGCGCAAGTTTATAGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATACGGCGTTTACGCCGCATCCGTCAAGAAGACATATGCAGGTTTTCACTAATCCACTACAGGACa                                                                                                   >  1:112615/1‑149 (MQ=255)
                                                 gTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAt                                                                                                  >  1:1311244/1‑149 (MQ=255)
                                                  tataAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAtt                                                                                                 >  2:897798/1‑149 (MQ=255)
                                                   ataAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAttt                                                                                                >  2:1488604/1‑149 (MQ=255)
                                                    taAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCACAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAtttt                                                                                               >  1:1339975/1‑149 (MQ=255)
                                                      atcttcccGAATCGCAATTTTATCCGGTCTATGGGGTCTATCGCAAATTAACTATTTTGGGATCACTTCTAGGCCGGATAAGGCGTTTACGCCTCATCCGTCAAGAAGACATATTCACTTTGTCACTAATCCACTACAGGACATTTTAt                                                                                             <  1:234040/144‑1 (MQ=255)
                                                      aGTTGCCCTATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTAt                                                                                             <  1:1384836/149‑1 (MQ=255)
                                                      aGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTAt                                                                                             <  1:690831/149‑1 (MQ=255)
                                                      aGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTAt                                                                                             <  2:1271257/149‑1 (MQ=255)
                                                         tGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGAt                                                                                          <  2:1322172/149‑1 (MQ=255)
                                                           cccGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCa                                                                                        <  2:1243103/149‑1 (MQ=255)
                                                           cccGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCa                                                                                        <  2:1488851/149‑1 (MQ=255)
                                                              gATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGtt                                                                                     >  1:925121/1‑149 (MQ=255)
                                                                tGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCa                                                                                   <  1:1092280/149‑1 (MQ=255)
                                                                  cgcAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCAtt                                                                                 <  1:20482/149‑1 (MQ=255)
                                                                   gcAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTa                                                                                <  1:686765/149‑1 (MQ=255)
                                                                    cAAGTTTATCGGGTCTATGGGGGCACTCGCAATTTATAGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCAATACAGGACATTTTATGATCAGTTCATTAt                                                                               >  1:969480/1‑149 (MQ=255)
                                                                    cAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTAt                                                                               <  2:1561213/149‑1 (MQ=255)
                                                                        tttATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAATGCGTTTACGCCGCATCCGGCTAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGAt                                                                           >  1:210199/1‑149 (MQ=255)
                                                                         ttATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATc                                                                          >  2:825762/1‑149 (MQ=255)
                                                                          tATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCg                                                                         >  2:419793/1‑149 (MQ=255)
                                                                            tCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGcc                                                                       >  2:1049945/1‑149 (MQ=255)
                                                                               ggTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaaa                                                                    >  2:623605/1‑149 (MQ=255)
                                                                                gTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaaaa                                                                   >  1:696428/1‑149 (MQ=255)
                                                                                gTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaaaa                                                                   <  1:663883/149‑1 (MQ=255)
                                                                                        gggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCtt                                                           >  2:766992/1‑149 (MQ=255)
                                                                                        gggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCtt                                                           >  1:1326199/1‑149 (MQ=255)
                                                                                           cAATCGGAATTTATAGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTATGCCGCATCCGGCAAGAAGACATTTGCACTTTGTCACTAATCCACTACAGGACATTTTATTATCAGTTCATTATGGATCGCCAATACGGGCCTTGAc                                                        <  2:258849/149‑1 (MQ=39)
                                                                                           cAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGAc                                                        >  2:1137450/1‑149 (MQ=255)
                                                                                           cAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGAc                                                        <  2:1501674/149‑1 (MQ=255)
                                                                                              tCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGcc                                                     >  1:1314018/1‑149 (MQ=255)
                                                                                                 cAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGACACAACGGGCCTTGACGCCCAg                                                  >  1:135804/1‑149 (MQ=255)
                                                                                                     ttATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCaaa                                              <  1:1278682/149‑1 (MQ=255)
                                                                                                          gATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAat                                         <  1:1488604/149‑1 (MQ=255)
                                                                                                           aTTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAata                                        >  1:915097/1‑149 (MQ=255)
                                                                                                             tttGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATg                                      <  1:1430722/149‑1 (MQ=255)
                                                                                                                gCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGAc                                   <  1:804622/149‑1 (MQ=255)
                                                                                                                      aCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCAtt                             >  2:1498916/1‑149 (MQ=255)
                                                                                                                            ttgCCGGATGCGGCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaac                       >  2:222839/3‑149 (MQ=255)
                                                                                                                            aGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaac                       >  2:5890/1‑149 (MQ=255)
                                                                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaaca                      >  2:1295472/1‑149 (MQ=255)
                                                                                                                              gCCGGATGCGGCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTGTATGATCAGTTCATTATGGATCGCCAAACCGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaacaa                     >  2:450668/1‑149 (MQ=255)
                                                                                                                               ccGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCaacaac                    <  1:1295472/149‑1 (MQ=255)
                                                                                                                                cGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGTCATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc                   >  1:974203/1‑149 (MQ=255)
                                                                                                                                cGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTTACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc                   >  1:1393677/1‑149 (MQ=255)
                                                                                                                                cGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc                   >  1:1195237/1‑149 (MQ=255)
                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg                 <  1:999411/149‑1 (MQ=255)
                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg                 <  2:1311244/149‑1 (MQ=255)
                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg                 >  2:1289461/1‑149 (MQ=255)
                                                                                                                                  gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg                 <  1:409721/149‑1 (MQ=255)
                                                                                                                                   atgcggCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTgg                <  2:747616/145‑1 (MQ=255)
                                                                                                                                   aTAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTgg                >  1:1546856/1‑149 (MQ=255)
                                                                                                                                     aaGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCa              >  2:30637/1‑149 (MQ=255)
                                                                                                                                        gcgtaaaCGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAAc           <  1:450668/143‑1 (MQ=255)
                                                                                                                                            ttACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCa       <  2:1195237/149‑1 (MQ=255)
                                                                                                                                             tACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAg      <  1:1127572/149‑1 (MQ=255)
                                                                                                                                             tACGCCGCATCCGGCAAGAAGACATATGCACTTTGGCACTAATCCACTACATGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTCGCAAACGTCAg      <  2:112615/149‑1 (MQ=255)
                                                                                                                                                gccgcATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTAc   <  1:1599830/149‑1 (MQ=255)
                                                                                                                                                 ccgcATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACt  <  2:526056/149‑1 (MQ=255)
                                                                                                                                                 ccgcATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACt  >  2:453253/1‑149 (MQ=255)
                                                                                                                                                 ccgcATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACt  <  1:1509111/149‑1 (MQ=255)
                                                                                                                                                 ccgcATCCGGCAAGAAGACATATGCACTTTGTCAATAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACt  <  2:17249/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACT  >  NC_000913/1134528‑1134821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: