Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,226,114 C→G 7.6% intergenic (‑34/‑486) minC ← / → ycgJ inhibitor of FtsZ ring polymerization/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,226,1140CG 7.6% 371.1 / 17.1 117intergenic (‑34/‑486)minC/ycgJinhibitor of FtsZ ring polymerization/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (68/40);  new base G (6/3);  total (74/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

Only 100 of 120 total aligned reads displayed.

CTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGTCAATCAA  >  NC_000913/1225967‑1226261
                                                                                                                                                   |                                                                                                                                                   
cTGAGCGATTTTGTCTTCCAGCGCCTGATGGAAAACCTTAGGTTCTGCCTCATGGAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATAAGCTATTAATCATCGCCa                                                                                                                                                    <  1:274733/149‑1 (MQ=255)
 tGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAg                                                                                                                                                   >  1:1547994/1‑149 (MQ=255)
       aTTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGcgcgcg                                                                                                                                             >  1:948163/1‑149 (MQ=255)
        ttttGTCTTCCAGCGCCTGATGTATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACAACCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCga                                                                                                                                            >  1:283643/1‑149 (MQ=255)
          ttGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCgatg                                                                                                                                          >  2:929184/1‑149 (MQ=255)
           tGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCgatga                                                                                                                                         >  2:585246/1‑149 (MQ=255)
            gTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCGCGCTGGCgatgat                                                                                                                                        <  1:1025152/149‑1 (MQ=255)
            gTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCGCGCTGGCgatgat                                                                                                                                        <  2:353927/149‑1 (MQ=255)
               ttCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGtt                                                                                                                                     >  1:1166286/1‑149 (MQ=255)
                tCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTc                                                                                                                                    >  2:534617/1‑149 (MQ=255)
                    gcgcCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGaa                                                                                                                                <  1:81573/149‑1 (MQ=255)
                    gcgcCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGaa                                                                                                                                <  2:1540811/149‑1 (MQ=255)
                          gATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTCTATGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACtat                                                                                                                          >  2:1544359/1‑149 (MQ=255)
                              gATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAgg                                                                                                                      <  1:413632/149‑1 (MQ=255)
                              gATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAgg                                                                                                                      >  1:18680/1‑149 (MQ=255)
                              gATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAgg                                                                                                                      >  2:479390/1‑149 (MQ=255)
                               aTAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGATACTGCCTTTAAGCTCGAGTTGCGTGTTTGAGATCCTGGCCTTACTCACTTCGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGc                                                                                                                     <  2:219798/149‑1 (MQ=255)
                                    cTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGtt                                                                                                                >  2:261531/1‑149 (MQ=255)
                                    cTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGtt                                                                                                                >  1:981758/1‑149 (MQ=255)
                                    cTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGtt                                                                                                                >  1:1521751/1‑149 (MQ=255)
                                          ttCTGCCTCATGCAGATTAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTc                                                                                                          >  1:363002/1‑149 (MQ=255)
                                            cTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTg                                                                                                        <  1:549034/149‑1 (MQ=255)
                                            cTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATAATGTTCCGAAGACTATAAGGCATGTTATAGTCTg                                                                                                        >  2:254628/1‑149 (MQ=255)
                                              gCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGa                                                                                                      <  1:698606/149‑1 (MQ=255)
                                               ccTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAt                                                                                                     >  2:114066/1‑149 (MQ=255)
                                               ccTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAt                                                                                                     >  1:669027/1‑149 (MQ=255)
                                               ccTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAt                                                                                                     >  2:1492980/1‑149 (MQ=255)
                                                cTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTc                                                                                                          >  2:300017/1‑143 (MQ=255)
                                                cTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAtt                                                                                                    >  2:296089/1‑149 (MQ=255)
                                                   aTGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATtata                                                                                                 <  1:1340078/149‑1 (MQ=255)
                                                   aTGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGATCCGAAGACTATAAGGCATGTTATAGTCTGGATtata                                                                                                 >  1:1520004/1‑149 (MQ=255)
                                                       aGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGa                                                                                             >  1:1592194/1‑149 (MQ=255)
                                                       aGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGa                                                                                             <  1:1165663/149‑1 (MQ=255)
                                                       aGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGa                                                                                             <  1:1584065/149‑1 (MQ=255)
                                                       aGATGAACCACAGATAAAGTGAAGCTACTGCCTATAAGCTAGATTGGCGTGATTCACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGTCATGATATAGTCTGGATTATATTGa                                                                                             >  1:831319/1‑149 (MQ=255)
                                                        gATGAACCTCAGATAAAGTGAAGCTACTGCCTTTAAGCTAGATTGGCGTGTTTGACATCCTGGCCTTACTCAATAAGCTATTAATCATCGGCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAg                                                                                            <  1:1432992/149‑1 (MQ=255)
                                                              ccACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGt                                                                                      >  2:817539/1‑149 (MQ=255)
                                                              ccACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGt                                                                                      >  2:659708/1‑149 (MQ=255)
                                                              ccACAGATAAAGTGAAGCTACTGCCATTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGt                                                                                      >  1:1240226/1‑149 (MQ=255)
                                                               cacaGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTc                                                                                     <  1:59177/149‑1 (MQ=255)
                                                               cacaGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTc                                                                                     <  1:659708/149‑1 (MQ=255)
                                                                 caGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCAc                                                                                   >  2:708545/1‑149 (MQ=255)
                                                                 caGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCAc                                                                                   >  1:1474812/1‑149 (MQ=255)
                                                                    aTAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCt                                                                                >  1:642442/1‑149 (MQ=255)
                                                                    aTAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCGGGCCGTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCt                                                                                >  2:309958/1‑149 (MQ=255)
                                                                     tAAGGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTc                                                                               >  1:1199434/1‑149 (MQ=255)
                                                                       aaGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTccc                                                                             <  1:1222595/149‑1 (MQ=255)
                                                                         gTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCAt                                                                           <  2:1081713/149‑1 (MQ=255)
                                                                           gAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCAttt                                                                         >  2:463370/1‑149 (MQ=255)
                                                                            aaGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTa                                                                        <  2:1547994/149‑1 (MQ=255)
                                                                              gCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCAATACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTAtt                                                                      >  2:1195682/1‑149 (MQ=255)
                                                                               cTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTc                                                                     <  1:1236307/149‑1 (MQ=255)
                                                                                tACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCTCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCa                                                                    <  2:742519/149‑1 (MQ=255)
                                                                                tACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCa                                                                    <  2:1199434/149‑1 (MQ=255)
                                                                                tACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCa                                                                    <  1:463370/149‑1 (MQ=255)
                                                                                  cTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCaga                                                                  >  1:833015/1‑149 (MQ=255)
                                                                                  cTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATAATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCaga                                                                  <  1:254628/149‑1 (MQ=255)
                                                                                   tGCCTTTAAGCTCGATTGGAGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCTCGATATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCagag                                                                 <  1:588579/149‑1 (MQ=255)
                                                                                     ccTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTa                                                               >  1:1046976/1‑149 (MQ=255)
                                                                                     ccTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTa                                                               >  1:1220401/1‑149 (MQ=255)
                                                                                       tttAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaaa                                                             >  1:812517/1‑149 (MQ=255)
                                                                                       tttAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaaa                                                             >  1:225661/1‑149 (MQ=255)
                                                                                           aGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTc                                                         >  1:934491/1‑149 (MQ=255)
                                                                                            gCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTAtt                                                                      >  1:1494081/1‑135 (MQ=255)
                                                                                            gCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTAtt                                                                      <  2:1494081/135‑1 (MQ=255)
                                                                                             cTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTAAGAGTAAAAGTCTa                                                       >  1:191589/1‑149 (MQ=255)
                                                                                                    ggCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCtgtg                                                <  1:929184/149‑1 (MQ=255)
                                                                                                     gCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGa                                               >  1:1587070/1‑149 (MQ=255)
                                                                                                             gACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGgc                                       <  1:534617/149‑1 (MQ=255)
                                                                                                              aCATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAGGTCTATTCTGTGATAAATGgcg                                      >  1:450449/1‑149 (MQ=255)
                                                                                                              aCATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGgcg                                      >  2:1457333/1‑149 (MQ=255)
                                                                                                              aCATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGAATGTTATAGTCTGGATTATATTGAGGCAAGTCACACTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGgcg                                      >  2:543145/1‑149 (MQ=255)
                                                                                                                  ccTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTgt                                  >  2:378313/1‑148 (MQ=255)
                                                                                                                   cTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGAt                                 >  1:129173/1‑149 (MQ=255)
                                                                                                                   cTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGAt                                 <  2:1592194/149‑1 (MQ=255)
                                                                                                                    tGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGAtt                                <  2:669027/149‑1 (MQ=255)
                                                                                                                     atcgatACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTc                               <  2:1000510/144‑1 (MQ=255)
                                                                                                                            cTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCTCCCTCGCATTTATTCAGAATAAAAGTCATTTATATGATAAATGTCGCTGATTGATAGAtt                        >  2:768465/1‑149 (MQ=255)
                                                                                                                             tCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTTAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGATTa                       >  1:963942/1‑149 (MQ=255)
                                                                                                                               aaTTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTaaa                     >  1:1005985/1‑149 (MQ=255)
                                                                                                                                    gCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATa                >  2:823988/1‑149 (MQ=255)
                                                                                                                                    gCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATa                <  1:1142176/149‑1 (MQ=255)
                                                                                                                                      ggaacatcatcGCGCGCTGGCGATGATGTTCCGAAGCCTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAcc              >  1:1440755/6‑149 (MQ=255)
                                                                                                                                       aTTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAccc             <  2:646917/149‑1 (MQ=255)
                                                                                                                                       aTTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAccc             >  1:103351/1‑149 (MQ=255)
                                                                                                                                       aTTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAacc             >  1:1254687/1‑149 (MQ=255)
                                                                                                                                         acatcatcGCGCGCTGGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCtt           >  2:1234979/3‑149 (MQ=255)
                                                                                                                                         acatcatcGCGCGCTGGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCtt           >  2:1414798/3‑149 (MQ=255)
                                                                                                                                         acatcatcGCGCGCTGGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGATGATTCATAGCTTAAAAAAAACCCtt           >  2:861454/3‑149 (MQ=255)
                                                                                                                                           atcatcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGt         <  2:1182486/149‑1 (MQ=255)
                                                                                                                                           atcatcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGt         <  2:1247436/149‑1 (MQ=255)
                                                                                                                                            tcatcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtc        <  2:18680/149‑1 (MQ=255)
                                                                                                                                            tcatcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTAAAGTCTGGATTATATTGAGGCAAGTCACCCACCCATTTATACAGAGTAAAAGACTAATCTGTGATAAATCGCGCTGATTCATAGCTTAAAAAATACCCTTGtc        >  1:567115/1‑149 (MQ=255)
                                                                                                                                            tcatcGCCAGCGCGCGATGATGTTCCGAAGACATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtca       >  2:1557567/1‑149 (MQ=255)
                                                                                                                                              atcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaa      >  1:1555164/1‑149 (MQ=255)
                                                                                                                                               tcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaat     <  1:261531/149‑1 (MQ=255)
                                                                                                                                               tcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaat     >  1:1420926/1‑149 (MQ=255)
                                                                                                                                                  ccAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaatcaa  >  1:1549233/1‑149 (MQ=255)
                                                                                                                                                  ccAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaatcaa  >  2:1533275/1‑149 (MQ=255)
                                                                                                                                                  ccAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGGTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTGATTCAGAGTAAAAGTGTATTCTGTGATAAATGGCGCGGAGGCATAGCTTAAAAAATACCCTTGtgaatcac  >  2:1496466/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGTCAATCAA  >  NC_000913/1225967‑1226261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: