Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,239,604 | C→T | 7.0% | V242V (GTC→GTT) | dadX → | alanine racemase, catabolic, PLP‑binding |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,239,604 | 0 | C | T | 7.0% | 175.4 / 2.7 | 57 | V242V (GTC→GTT) | dadX | alanine racemase, catabolic, PLP‑binding |
Reads supporting (aligned to +/- strand): ref base C (30/23); new base T (2/2); total (32/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
CGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTG > NC_000913/1239456‑1239751 | cggcgACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTc < 1:881737/149‑1 (MQ=255) ggcgACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTcc < 1:1342987/149‑1 (MQ=255) gcgACTCTGTGGCACCCGGAAGCGCAGTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGAGATATCGCCAATACCGGATTACGTCCCGTGATGACGCGAAGCAGTGAGATTATTGGTGTCCa > 2:202800/1‑149 (MQ=255) gACGCTGTGGCACCCGGAAGCGCATTTTGTCTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGa < 1:1590165/149‑1 (MQ=255) gtgGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTaa > 1:209913/1‑149 (MQ=255) tgGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTaaa > 2:773755/1‑149 (MQ=255) gCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAg > 2:235339/1‑149 (MQ=255) gcATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCgtg < 2:648623/149‑1 (MQ=255) aTTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGtgtg > 2:399363/1‑149 (MQ=255) ttttGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTgg < 2:39929/149‑1 (MQ=255) tttGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTggg < 1:1070216/149‑1 (MQ=255) ttGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGGCCAGACGCTAAAAGCGGGCGAGCGTGTGTGc < 2:1368227/149‑1 (MQ=255) ggTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTAcggcg > 2:1489473/1‑149 (MQ=255) gTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTAcggcgg > 1:922353/1‑149 (MQ=255) gtCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGt < 2:1529438/148‑1 (MQ=255) cTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCtat < 2:1503494/149‑1 (MQ=255) ggCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATAc > 2:1568549/1‑149 (MQ=255) cATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTTCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTg > 2:570661/1‑149 (MQ=255) cATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTTCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTg > 2:565082/1‑149 (MQ=255) attTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTgcgcg > 2:292759/1‑149 (MQ=255) ttttGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTgcgcgc > 1:1569964/1‑149 (MQ=255) ttgaaacgcgcTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATAACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCAAAAAGCGGGCGAGCGTGTGGGCTACGGCGGACGCTATACTGCGCGCGa < 2:1077177/142‑1 (MQ=255) ttGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGa < 1:235339/149‑1 (MQ=255) ttGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGa < 1:1517893/149‑1 (MQ=255) tGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTTCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAAc < 1:570661/149‑1 (MQ=255) tGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTTCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAAc < 1:565082/149‑1 (MQ=255) cgcTTCGCCGTCCGGTCAGGGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATTAACAGc < 1:1132355/149‑1 (MQ=255) cGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGTTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATc > 1:552369/1‑149 (MQ=255) ccGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCCCGATGAACAGCGAATCgg > 1:1053393/1‑149 (MQ=255) tCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCAt < 1:1489473/149‑1 (MQ=255) gTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTc > 2:662887/1‑149 (MQ=255) tCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTcg > 2:220589/1‑149 (MQ=255) tCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGGGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTcg < 1:773755/149‑1 (MQ=255) cAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGGCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTcgc < 2:995386/149‑1 (MQ=255) tGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGCGCGTGTGGGCTAAGGCGGTCGCTATACTGCGCTCGATGACCAGAGAATAGGCATTATcgcagc > 2:291689/1‑149 (MQ=255) tGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTcgccgc > 2:1516547/1‑149 (MQ=255) ggCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCa > 1:1597869/1‑149 (MQ=255) gCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAg > 1:14184/1‑149 (MQ=255) gCGTGATATCGCCAATACCGGATTACGTCCGGGGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAg < 1:929348/149‑1 (MQ=255) cGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACATCGAATCGGCATTGTCGCCGCAgg > 1:101416/1‑149 (MQ=255) gATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTa > 2:692834/1‑149 (MQ=255) cAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACg < 1:1568549/149‑1 (MQ=255) gATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCcg > 2:1064271/1‑149 (MQ=255) gTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTAGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGAATTGTCGCCGCAGGGTACGCCGACGGTTATCCTCGCCACGCTcc > 2:800633/1‑149 (MQ=255) tgatgaCGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCt < 2:1145770/149‑1 (MQ=255) tgaCGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTAcc > 1:1343167/1‑149 (MQ=255) gaCGCTAAGCAGTGAGAATATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCg < 1:292759/149‑1 (MQ=255) aCGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCgg < 2:376869/149‑1 (MQ=255) gCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTa > 1:594213/1‑149 (MQ=255) tAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTAcc > 2:1257343/1‑149 (MQ=255) gTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGtt < 2:2816/149‑1 (MQ=255) gaTTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAg < 2:933911/149‑1 (MQ=255) gaTAATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTTTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAg < 2:1077696/149‑1 (MQ=255) tGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACg > 2:1291019/1‑149 (MQ=255) tGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGGTTTAGTGGACg > 1:70518/1‑149 (MQ=255) gtgtCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCTCAATACTGATAGCGATAAACATCTAATAGGCATTGTCGCCTCAGGGTACGCCTAAGGTAATCCGCGCCACGCGCCTACCGGTAACCCTGTTATAGTCGAAGGc > 2:1453763/1‑149 (MQ=255) gtgtCCAGACGCAAAAAGCGGGCGAGCGTGTGGGCAACGGCGGTCGCTATACTGCGCACTATGAACAGCGAATCGCCATTGTCGCCGCAGTGTACGCCGACGGTAATCCTCGCAACGCGCATAACGGTAACCCTATTTTAGTGGACGGc > 2:157960/1‑149 (MQ=255) tCCAGACGCTAAAAGCGGGCGAGCGTGTGGGGTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTg < 2:552369/149‑1 (MQ=255) tCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGAGATGAACAGCGAATCGGCATTGTCGCCTCAGGGTACGCCGACGGATATCCGCGCCACGCGCCTACAGGTACCCCTGTTTTAGTGGACGGCGTg > 1:397103/1‑149 (MQ=255) | CGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATGGCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATTGGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAACAGCGAATCGGCATTGTCGCCGCAGGGTACGCCGACGGTTATCCGCGCCACGCGCCTACCGGTACCCCTGTTTTAGTGGACGGCGTG > NC_000913/1239456‑1239751 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |