Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,250,964 G→A 5.4% intergenic (‑126/‑102) dhaK ← / → dhaR dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit/dhaKLM operon transcription activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,250,9640GA 5.4% 410.9 / 7.2 129intergenic (‑126/‑102)dhaK/dhaRdihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit/dhaKLM operon transcription activator
Reads supporting (aligned to +/- strand):  ref base G (62/59);  new base A (5/2);  total (67/62)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01

Only 100 of 134 total aligned reads displayed.

ACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAAT  >  NC_000913/1250816‑1251112
                                                                                                                                                    |                                                                                                                                                    
acaTCATTGATCAATTTTTTCATTATTTTGATCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAg                                                                                                                                                      <  2:694673/149‑1 (MQ=255)
  atcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTATGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTAATAGTg                                                                                                                                                    >  2:1546029/1‑149 (MQ=255)
  atcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGAAACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGATTCATAGTg                                                                                                                                                    >  1:296605/1‑149 (MQ=255)
   tcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGa                                                                                                                                                   <  2:1352194/149‑1 (MQ=255)
     atTGATCAATATTTTCATTATTTTGCTCCAGCAATTACGCTAGGGCATGGATGATTTTCAACTACACGGAGACCACTGAATGCCGATTAATACATTGTGCATCATCGAATGGAAAAGAAAATTATCCCAAATTATGTTTCATAATGaaa                                                                                                                                                 >  1:237339/1‑149 (MQ=255)
      ttGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAAc                                                                                                                                                >  2:595145/1‑149 (MQ=255)
        gATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACat                                                                                                                                              >  2:1180911/1‑149 (MQ=255)
         aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACata                                                                                                                                             >  1:1368043/1‑149 (MQ=255)
         aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACata                                                                                                                                             >  1:842715/1‑149 (MQ=255)
          tCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACatat                                                                                                                                            >  2:1145426/1‑149 (MQ=255)
            aaTTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATTTTTCTTAGTGAAACATATgg                                                                                                                                          >  1:1533230/1‑148 (MQ=255)
            aaTTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGc                                                                                                                                          <  2:83546/149‑1 (MQ=255)
             aTTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCt                                                                                                                                         <  1:922484/149‑1 (MQ=255)
              ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCACTATGAAACataatttg                                                                                                                                        <  2:972426/149‑6 (MQ=255)
              ttttttCATTATTTTGCTCCAGCAATTACGCTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCtt                                                                                                                                        >  1:1117184/1‑149 (MQ=255)
                ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCACTATGAAACataatttggg                                                                                                                                      <  1:648686/149‑8 (MQ=255)
                 tttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTaa                                                                                                                                     <  2:564887/149‑1 (MQ=255)
                 tttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGAGGATGTTCAACGACACGGCTACCACTGACTGCCGATGATTCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATCTGTCATAGTGATAAATATGCTTTaa                                                                                                                                     >  2:1036812/1‑149 (MQ=255)
                  ttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTaat                                                                                                                                    <  2:1469616/149‑1 (MQ=255)
                  tacATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTaat                                                                                                                                    <  1:263935/147‑1 (MQ=255)
                      ttattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTaatgaat                                                                                                                                <  2:691741/149‑1 (MQ=255)
                           ttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                                                           <  1:526301/149‑1 (MQ=255)
                             gCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCat                                                                                                                         <  2:485073/149‑1 (MQ=255)
                              cTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCata                                                                                                                        <  2:778876/149‑1 (MQ=255)
                                caaGCAATTATGGTATGTCATGGATGATGTTCAACGACACGGCGACCACTGACTTCCGATGAATCCATTGTTCATCAGGCAAGTGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATAtt                                                                                                                      <  1:1546029/147‑1 (MQ=255)
                                 cAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATAtt                                                                                                                      >  2:547769/1‑148 (MQ=255)
                                 cAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                                                     >  2:187648/1‑149 (MQ=255)
                                      aTTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTaat                                                                                                                                    <  2:1111654/129‑1 (MQ=255)
                                      aTTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTaat                                                                                                                                    <  2:83507/129‑1 (MQ=255)
                                      aTTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTaat                                                                                                                                    >  1:1111654/1‑129 (MQ=255)
                                      aTTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACt                                                                                                                <  2:893966/149‑1 (MQ=255)
                                       ttACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCAGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCACAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTTAAACtt                                                                                                               >  1:1127293/1‑149 (MQ=255)
                                        tACGGTAGGGGATGGTTGATGTTAAACGACACGGGGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGACCAAATTATTTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                                                              <  2:968320/149‑1 (MQ=255)
                                        tACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                                                              <  2:842715/149‑1 (MQ=255)
                                         aCGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACtttt                                                                                                             >  1:985680/1‑149 (MQ=255)
                                             tAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                                                         >  1:887429/1‑149 (MQ=255)
                                               gggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtgt                                                                                                       <  2:346272/149‑1 (MQ=255)
                                                   aTGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                                                   >  1:1454956/1‑149 (MQ=255)
                                                    tGGATGATGTTAAACGACACGGCGAACACTTACTGCCGATGAATCCATTGTGCATCAGGCAAGGTAAAAGAAAATTAGCACAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATTTTGAAACTTTTACGTGTATTaa                                                                                                  <  2:1279501/149‑1 (MQ=255)
                                                       atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATAc                                                                                               <  1:216593/149‑1 (MQ=255)
                                                           tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaa                                                                                           >  2:768050/1‑149 (MQ=255)
                                                           tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaa                                                                                           >  1:815444/1‑149 (MQ=255)
                                                            gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaat                                                                                          >  1:578093/1‑148 (MQ=255)
                                                                aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                                                            <  1:305720/127‑1 (MQ=255)
                                                                aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                                                            >  2:305720/1‑127 (MQ=255)
                                                                   tacacGTAGACCACTGACTGACGATGAATCCATTTTGCATCATTGAAGGTAAAAGAATATTAGACAAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGa                                                                                   <  1:74225/148‑1 (MQ=255)
                                                                         gCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGa                                                                             >  1:1410993/1‑149 (MQ=255)
                                                                          cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                                                            <  1:1533505/149‑1 (MQ=255)
                                                                          cGACCACTGACTGCCGATGAATCCATTGTGCATAAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                                                            <  2:18058/149‑1 (MQ=255)
                                                                          cGACCACTGACTGCCGATGAATACATTTTGCATCAGGAAAGTGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                                                            <  2:1127293/149‑1 (MQ=255)
                                                                          cGACCAATGACTGCATATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATTTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                                                            <  2:215464/149‑1 (MQ=255)
                                                                              cACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                                                        >  1:891128/1‑149 (MQ=255)
                                                                              cACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTAACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                                                        >  1:823377/1‑149 (MQ=255)
                                                                              aactgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                                                        <  1:246262/148‑1 (MQ=255)
                                                                                ctgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                                                      >  2:11260/1‑149 (MQ=255)
                                                                                 tgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCttt                                                                     <  1:699546/149‑1 (MQ=255)
                                                                                  gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtttt                                                                    <  2:1337400/149‑1 (MQ=255)
                                                                                    ctgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGt                                                                  <  2:778346/149‑1 (MQ=255)
                                                                                    ctgCAGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATTTTTCAAAGTGAAACATTTGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGt                                                                  <  2:304912/149‑1 (MQ=255)
                                                                                     tgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATCGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTc                                                                 >  1:1100248/1‑149 (MQ=255)
                                                                                     tgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATCGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTc                                                                 >  1:1094360/1‑149 (MQ=255)
                                                                                       ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                                                               >  2:1217373/1‑149 (MQ=255)
                                                                                       ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                                                               >  1:553109/1‑149 (MQ=255)
                                                                                       ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                                                               >  1:534217/1‑149 (MQ=255)
                                                                                         gATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATaa                                                             <  1:1329596/149‑1 (MQ=255)
                                                                                               tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                                                            >  2:620617/1‑128 (MQ=255)
                                                                                               tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                                                            <  1:620617/128‑1 (MQ=255)
                                                                                               tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                                                       >  1:1206456/1‑149 (MQ=255)
                                                                                                 cATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCg                                                     >  1:1065612/1‑149 (MQ=255)
                                                                                                  aTTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCgg                                                    <  1:1274191/149‑1 (MQ=255)
                                                                                                  aTTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCgg                                                    >  2:1502491/1‑149 (MQ=255)
                                                                                                  aTTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCgg                                                    >  1:1224787/1‑149 (MQ=255)
                                                                                                      tgCATCATGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGata                                                <  2:1533230/149‑1 (MQ=255)
                                                                                                        cATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGatatt                                              >  1:415876/1‑148 (MQ=255)
                                                                                                         aTCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGa                                             >  1:177008/1‑149 (MQ=255)
                                                                                                         aTCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGa                                             >  1:344564/1‑149 (MQ=255)
                                                                                                            aGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg                                          >  2:127212/1‑149 (MQ=255)
                                                                                                             ggCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACTTATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTgg                                         <  2:1368043/149‑1 (MQ=255)
                                                                                                                aaGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTACATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcgc                                      >  2:1049574/1‑149 (MQ=255)
                                                                                                                  gggAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCtt                                    >  2:1560619/1‑149 (MQ=255)
                                                                                                                   ggAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCttt                                   >  1:1294650/1‑149 (MQ=255)
                                                                                                                       aagaaaaTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGATACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACCCCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTaac                               >  1:808354/1‑149 (MQ=255)
                                                                                                                             aTTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGAt                         <  1:578914/149‑1 (MQ=255)
                                                                                                                                 gCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTc                     >  1:651605/1‑149 (MQ=255)
                                                                                                                                 gCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGATTTTAAAAACGATGGTc                     >  2:431248/1‑149 (MQ=255)
                                                                                                                                 gCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGAGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTc                     <  2:553109/149‑1 (MQ=255)
                                                                                                                                   ccAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCAAATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCgg                   >  2:260839/1‑149 (MQ=255)
                                                                                                                                    aagcatATGTTTCACTATGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCggg                  >  2:678622/6‑149 (MQ=255)
                                                                                                                                     aaaTTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCgggg                 <  1:448999/149‑1 (MQ=255)
                                                                                                                                       catATGTTTCACTATGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCa               >  2:864141/3‑149 (MQ=255)
                                                                                                                                        ttATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCTATACGTAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATTTTCGGGGCat              >  1:332334/1‑149 (MQ=255)
                                                                                                                                         tATGTTTCACTATGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCata             >  2:1240087/1‑149 (MQ=255)
                                                                                                                                            ttttCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATAtct          <  2:1065612/148‑1 (MQ=255)
                                                                                                                                             tttCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATAtctc         >  1:1436323/1‑149 (MQ=255)
                                                                                                                                                cATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCt      <  1:171510/149‑1 (MQ=255)
                                                                                                                                                 aTAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCtt     <  2:693335/149‑1 (MQ=255)
                                                                                                                                                 aTAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCtt     <  2:1454956/149‑1 (MQ=255)
                                                                                                                                                   aGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTaa   <  2:865541/149‑1 (MQ=255)
                                                                                                                                                    gTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAAt  >  1:720100/1‑149 (MQ=255)
                                                                                                                                                    gTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAAt  <  1:187648/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAAT  >  NC_000913/1250816‑1251112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: