Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,270,726 C→G 5.0% intergenic (+266/+23) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,270,7260CG 5.0% 402.1 / 9.5 120intergenic (+266/+23)rdlC/chaAsRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (50/63);  new base G (3/3);  total (54/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 124 total aligned reads displayed.

GCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCAT  >  NC_000913/1270578‑1270874
                                                                                                                                                     |                                                                                                                                                    
gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTAcc                                                                                                                                                      <  1:184337/149‑1 (MQ=255)
gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGGGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTAcc                                                                                                                                                      <  1:218183/149‑1 (MQ=255)
 cAATGAAGAGTACATTCCTGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCg                                                                                                                                                     <  1:720456/149‑1 (MQ=255)
   aTGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGGGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTc                                                                                                                                                   <  2:733965/149‑1 (MQ=255)
    tGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCa                                                                                                                                                  >  2:1266031/1‑149 (MQ=255)
     gAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTTGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCAt                                                                                                                                                 <  2:729677/149‑1 (MQ=255)
       agagTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCg                                                                                                                                               <  2:595731/149‑1 (MQ=255)
        gagTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCgg                                                                                                                                              >  2:264416/1‑149 (MQ=255)
         agTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCgga                                                                                                                                             <  2:40230/149‑1 (MQ=255)
            aCATTCCGGCTTTTCATCAGCGGTTGCAGTGGTTTCTTGCAGGGTGTTGTTGTGTCTAGCTATTCTGATTATCGACCTGTACAGGTATTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCggagga                                                                                                                                          <  2:427988/149‑1 (MQ=255)
            aCATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCggggga                                                                                                                                          <  2:1452686/149‑1 (MQ=255)
             cATTCCGTCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGTTACAGGATCGCAAATCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                                                                                         <  1:314268/149‑1 (MQ=255)
             cATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACTATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                                                                                         <  1:1542728/149‑1 (MQ=255)
              aTTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGgaga                                                                                                                                        <  1:539952/149‑1 (MQ=255)
              aTTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGgaga                                                                                                                                        >  1:790554/1‑149 (MQ=255)
              aTTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGgaga                                                                                                                                        >  1:1476389/1‑149 (MQ=255)
               ttCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGat                                                                                                                                       >  1:946293/1‑149 (MQ=255)
                tCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGata                                                                                                                                      <  1:225516/149‑1 (MQ=255)
                   ggCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCGGGGGAGataata                                                                                                                                   <  1:264416/149‑4 (MQ=39)
                   ggCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATaa                                                                                                                                   >  2:1209439/1‑149 (MQ=255)
                   ggCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATaa                                                                                                                                   <  1:510758/149‑1 (MQ=255)
                      ttttCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATAGGAGGAGATATAACTg                                                                                                                                >  1:1516317/1‑149 (MQ=255)
                                   ttGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATc                                                                                                                   >  2:577272/1‑149 (MQ=255)
                                     gCAGTGGTTTCGTGCAGGGTGTTGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGt                                                                                                                 <  1:1474271/149‑1 (MQ=255)
                                     gCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGt                                                                                                                 <  2:1060056/149‑1 (MQ=255)
                                     gCAGTGGGTTCGTGCAGGGTGTGGGACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGt                                                                                                                 <  2:888926/149‑1 (MQ=255)
                                           gTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCaa                                                                                                           >  2:839773/1‑149 (MQ=255)
                                            tttCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGACATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCaaa                                                                                                          >  1:394246/1‑149 (MQ=255)
                                                gTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAgg                                                                                                      >  1:1087038/1‑149 (MQ=255)
                                                 ttcAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggc                                                                                                     <  1:1524002/147‑1 (MQ=255)
                                                 tGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggc                                                                                                     >  2:476775/1‑149 (MQ=255)
                                                             tACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGc                                                                                         <  2:1277003/149‑1 (MQ=255)
                                                             tACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGc                                                                                         <  2:790554/149‑1 (MQ=255)
                                                               cAGGCTCTCAATTCTGATTAACGACCTCTCCAGGTTGTATGAAGTAATACAATACGCAGGAATTTATATAATCTCCCCCTATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGTCAAACAGTGCCa                                                                                       <  2:1160194/149‑1 (MQ=255)
                                                                  gCTCTCAATTCTGAATAACGACCTGTCCATGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAt                                                                                    <  2:1552303/149‑1 (MQ=255)
                                                                   cTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAGTACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtg                                                                                   >  1:1227510/1‑149 (MQ=255)
                                                                   cTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtg                                                                                   <  1:1043993/149‑1 (MQ=255)
                                                                    tCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgc                                                                                  >  1:1463703/1‑149 (MQ=255)
                                                                    tCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACAGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgc                                                                                  >  2:118197/1‑149 (MQ=255)
                                                                     cGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgcg                                                                                 <  2:889283/149‑1 (MQ=255)
                                                                      gCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                                                                                         >  2:217025/1‑92 (MQ=255)
                                                                      gCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                                                                                         <  1:217025/92‑1 (MQ=255)
                                                                               aTTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCAtt                                                                       >  2:1228263/1‑149 (MQ=255)
                                                                                 tAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGa                                                                     <  1:577272/149‑1 (MQ=255)
                                                                                   aCGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGc                                                                   <  1:527022/149‑1 (MQ=255)
                                                                                      aCCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACg                                                                >  2:1545968/1‑149 (MQ=255)
                                                                                       ccTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGt                                                               <  2:545999/149‑1 (MQ=255)
                                                                                        cTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGtt                                                              >  2:103512/1‑149 (MQ=255)
                                                                                         tGTCCAGGTAGTATGGAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTa                                                             <  2:312277/149‑1 (MQ=255)
                                                                                         tGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTa                                                             <  1:1316689/149‑1 (MQ=255)
                                                                                         tGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTCCCAGATGCGCTGCGCCATTGAGCACGTTa                                                             <  1:1052123/149‑1 (MQ=255)
                                                                                          gTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAg                                                            <  2:171862/149‑1 (MQ=255)
                                                                                           tCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGt                                                           >  1:253700/1‑149 (MQ=255)
                                                                                             cAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATACCTGTCAGGCAAATATAGTCATCAAATAGGCGGCAAACAGTGCCAGAAGCGCTGCGCCATTAATCACGTTAGTAc                                                         >  1:1515601/1‑149 (MQ=255)
                                                                                              aGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACg                                                        >  1:724420/1‑149 (MQ=255)
                                                                                              aGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACg                                                        >  1:226855/1‑149 (MQ=255)
                                                                                               ggTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGt                                                       >  2:342013/1‑149 (MQ=255)
                                                                                                gtagtaTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTc                                                      >  1:1117953/1‑149 (MQ=255)
                                                                                                gtagaaTGAAGAAATACAATAAGCAGGAATAAATATTATCTCCCCCGATTACCGTCATCGGAGGAGATAAAACTGTCAGGCAAATAACGAGATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCAATTGATCAAGTTAGTAAGTc                                                      >  1:967801/1‑149 (MQ=255)
                                                                                                    taTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGt                                                  <  1:1266031/149‑1 (MQ=255)
                                                                                                      tGAAGAAATACAGTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTgg                                                >  1:1487551/1‑149 (MQ=255)
                                                                                                      tGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTgg                                                >  1:438592/1‑149 (MQ=255)
                                                                                                       gaagaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCTCTGCGCCATTGAGCACGTTAGTACGTCCGGTggt                                               >  2:1436580/1‑148 (MQ=255)
                                                                                                        aagaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGgag                                              >  2:1267153/1‑149 (MQ=255)
                                                                                                          gaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGaa                                            <  2:1476389/149‑1 (MQ=255)
                                                                                                            aaTACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAgg                                          <  2:879637/149‑1 (MQ=255)
                                                                                                             aTACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGga                                         >  2:719368/1‑149 (MQ=255)
                                                                                                                cAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGat                                      <  1:1481091/149‑1 (MQ=255)
                                                                                                                 aaTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGata                                     >  1:181780/1‑149 (MQ=255)
                                                                                                                 aaTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGata                                     >  1:593630/1‑149 (MQ=255)
                                                                                                                  aTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGatat                                    <  1:581253/149‑1 (MQ=255)
                                                                                                                  aTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGatat                                    <  1:1148116/149‑1 (MQ=255)
                                                                                                                    aaGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATgg                                  <  2:1516317/149‑1 (MQ=255)
                                                                                                                      gCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGt                                                               >  2:852799/1‑118 (MQ=255)
                                                                                                                      gCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGt                                                               <  1:852799/118‑1 (MQ=255)
                                                                                                                      gCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGca                                >  1:687118/1‑149 (MQ=255)
                                                                                                                        aGGAATTTATATTATCTCCCCCCGATTACCGTCATAGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac                               <  2:662290/149‑1 (MQ=255)
                                                                                                                        aGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca                              >  2:1441240/1‑149 (MQ=255)
                                                                                                                        aGGAATTTATATTATCTCCCCCGATGACCGTCATCGGAGGAGATATAACTGTAAGGAAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTTCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca                              >  1:329052/1‑149 (MQ=255)
                                                                                                                          gAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc                            >  2:1074180/1‑149 (MQ=255)
                                                                                                                           aaTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa                           <  2:475197/149‑1 (MQ=255)
                                                                                                                            aTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc                          <  1:998638/149‑1 (MQ=255)
                                                                                                                             tttATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt                         >  2:1292114/1‑149 (MQ=255)
                                                                                                                                atatTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa                      <  1:1368102/149‑1 (MQ=255)
                                                                                                                                atatTATCTCCCCCGATTACCGTCATCGGAGGACATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa                      <  1:793575/149‑1 (MQ=255)
                                                                                                                                 ttatatCTCCTCCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag                     >  2:794205/4‑149 (MQ=39)
                                                                                                                                 tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag                     <  1:1209439/149‑1 (MQ=255)
                                                                                                                                  attatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga                    <  1:476775/149‑1 (MQ=255)
                                                                                                                                   ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag                   <  2:698547/149‑1 (MQ=255)
                                                                                                                                   ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag                   <  2:1227510/149‑1 (MQ=255)
                                                                                                                                   ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGCCACAGCACTAAagag                   >  1:1096464/1‑149 (MQ=255)
                                                                                                                                   ttatCTCCCCCGATTACCGACATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag                   <  2:394246/149‑1 (MQ=255)
                                                                                                                                     atCTCCTCCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCTTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGc                 <  1:794205/149‑1 (MQ=255)
                                                                                                                                     atCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGc                 <  1:1282077/149‑1 (MQ=255)
                                                                                                                                            cccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGTGCTGCGCAATTGATCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCAACAAt           >  1:36897/1‑149 (MQ=255)
                                                                                                                                              cGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATca         >  1:922455/1‑149 (MQ=255)
                                                                                                                                                aTTACCGTCATCGGAGGAGATATAACTGTCAGTCAAATATCGTCATAAAAAACGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATcacc       <  1:244072/149‑1 (MQ=255)
                                                                                                                                                  tACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATcaccac     <  2:1087038/149‑1 (MQ=255)
                                                                                                                                                    ccGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCAccacca   <  1:730388/149‑1 (MQ=255)
                                                                                                                                                     cGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCAt  >  2:908146/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCAT  >  NC_000913/1270578‑1270874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: