Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,270,729 C→A 5.0% intergenic (+269/+20) rdlC → / ← chaA sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,270,7290CA 5.0% 402.5 / 7.6 119intergenic (+269/+20)rdlC/chaAsRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC/calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (51/62);  new base A (3/3);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

Only 100 of 121 total aligned reads displayed.

ATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTC  >  NC_000913/1270581‑1270877
                                                                                                                                                     |                                                                                                                                                    
  gAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTTGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCAt                                                                                                                                                    <  2:729677/149‑1 (MQ=255)
  gAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCAt                                                                                                                                                    <  2:1161378/149‑1 (MQ=255)
     gagTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCgg                                                                                                                                                 >  2:264416/1‑149 (MQ=255)
      agTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCgga                                                                                                                                                <  2:40230/149‑1 (MQ=255)
         aCATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCggggga                                                                                                                                             <  2:1452686/149‑1 (MQ=255)
          cATTCCGTCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGTTACAGGATCGCAAATCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                                                                                            <  1:314268/149‑1 (MQ=255)
          cATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACTATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                                                                                            <  1:1542728/149‑1 (MQ=255)
           aTTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGgaga                                                                                                                                           <  1:533541/149‑1 (MQ=255)
           aTTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGgaga                                                                                                                                           >  1:790554/1‑149 (MQ=255)
           aTTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGgaga                                                                                                                                           <  1:539952/149‑1 (MQ=255)
            ttCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGat                                                                                                                                          >  1:946293/1‑149 (MQ=255)
             tCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGata                                                                                                                                         <  1:225516/149‑1 (MQ=255)
                ggCTTTTCAACAGCTGTTGCAGTGGTTTCGTTCATGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCATGTAGTATGAAGAAATACAATAAGAAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATaa                                                                                                                                      <  2:1352182/149‑1 (MQ=255)
                ggCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCGGGGGAGataata                                                                                                                                      <  1:264416/149‑4 (MQ=39)
                ggCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATaa                                                                                                                                      >  2:1209439/1‑149 (MQ=255)
                ggCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATaa                                                                                                                                      <  1:510758/149‑1 (MQ=255)
                   ttttCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATAGGAGGAGATATAACTg                                                                                                                                   >  1:1516317/1‑149 (MQ=255)
                                ttGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATc                                                                                                                      >  2:577272/1‑149 (MQ=255)
                                  gCAGTGGTTTCGTGCAGGGTGTTGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGt                                                                                                                    <  1:1474271/149‑1 (MQ=255)
                                  gCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGt                                                                                                                    <  2:1060056/149‑1 (MQ=255)
                                  gCAGTGGGTTCGTGCAGGGTGTGGGACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGt                                                                                                                    <  2:888926/149‑1 (MQ=255)
                                   cAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtc                                                                                                                   >  1:1277003/1‑149 (MQ=255)
                                      tGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtcatc                                                                                                                <  2:12412/149‑1 (MQ=255)
                                      tGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtcatc                                                                                                                <  2:398879/149‑1 (MQ=255)
                                        gTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCaa                                                                                                              >  2:839773/1‑149 (MQ=255)
                                         tttCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGACATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCaaa                                                                                                             >  1:394246/1‑149 (MQ=255)
                                             gTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAgg                                                                                                         >  1:1087038/1‑149 (MQ=255)
                                              tGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggc                                                                                                        >  2:476775/1‑149 (MQ=255)
                                                          tACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGc                                                                                            <  2:790554/149‑1 (MQ=255)
                                                          tACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGc                                                                                            <  2:1277003/149‑1 (MQ=255)
                                                           aCAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGcc                                                                                           >  2:176424/1‑149 (MQ=255)
                                                            cAGGCTCTCAATTCTGATTAACGACCTCTCCAGGTTGTATGAAGTAATACAATACGCAGGAATTTATATAATCTCCCCCTATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGTCAAACAGTGCCa                                                                                          <  2:1160194/149‑1 (MQ=255)
                                                                cTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAGTACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtg                                                                                      >  1:1227510/1‑149 (MQ=255)
                                                                cTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtg                                                                                      <  1:1043993/149‑1 (MQ=255)
                                                                 tCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgc                                                                                     >  1:1463703/1‑149 (MQ=255)
                                                                  cGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgcg                                                                                    <  2:889283/149‑1 (MQ=255)
                                                                   gCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                                                                                            >  2:217025/1‑92 (MQ=255)
                                                                   gCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGgaggag                                                                                                                                            <  1:217025/92‑1 (MQ=255)
                                                                            aTTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCAtt                                                                          >  2:1228263/1‑149 (MQ=255)
                                                                             ttAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg                                                                         >  1:629928/1‑149 (MQ=255)
                                                                                aCGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGc                                                                      <  1:527022/149‑1 (MQ=255)
                                                                                   aCCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACg                                                                   >  2:1545968/1‑149 (MQ=255)
                                                                                    ccTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGt                                                                  <  2:545999/149‑1 (MQ=255)
                                                                                     cTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGtt                                                                 >  2:103512/1‑149 (MQ=255)
                                                                                     cTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGtt                                                                 >  2:690449/1‑149 (MQ=255)
                                                                                      tGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTa                                                                <  1:413235/149‑1 (MQ=255)
                                                                                      tGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTa                                                                <  1:1316689/149‑1 (MQ=255)
                                                                                      tGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTCCCAGATGCGCTGCGCCATTGAGCACGTTa                                                                <  1:1052123/149‑1 (MQ=255)
                                                                                       gTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAg                                                               <  2:171862/149‑1 (MQ=255)
                                                                                        tCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGt                                                              >  1:253700/1‑149 (MQ=255)
                                                                                          cAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATACCTGTCAGGCAAATATAGTCATCAAATAGGCGGCAAACAGTGCCAGAAGCGCTGCGCCATTAATCACGTTAGTAc                                                            >  1:1515601/1‑149 (MQ=255)
                                                                                           aGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACg                                                           >  1:226855/1‑149 (MQ=255)
                                                                                            ggTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGt                                                          >  2:342013/1‑149 (MQ=255)
                                                                                             gtagtaTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTc                                                         >  1:1117953/1‑149 (MQ=255)
                                                                                             gtagaaTGAAGAAATACAATAAGCAGGAATAAATATTATCTCCCCCGATTACCGTCATCGGAGGAGATAAAACTGTCAGGCAAATAACGAGATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCAATTGATCAAGTTAGTAAGTc                                                         >  1:967801/1‑149 (MQ=255)
                                                                                                gtaTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCgg                                                      <  1:1545968/149‑1 (MQ=255)
                                                                                                 taTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGt                                                     <  1:1266031/149‑1 (MQ=255)
                                                                                                   tGAAGAAATACAGTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTgg                                                   >  1:1487551/1‑149 (MQ=255)
                                                                                                    gaagaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCTCTGCGCCATTGAGCACGTTAGTACGTCCGGTggt                                                  >  2:1436580/1‑148 (MQ=255)
                                                                                                     aagaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGgag                                                 >  2:751701/1‑149 (MQ=255)
                                                                                                     aagaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGgag                                                 >  2:1267153/1‑149 (MQ=255)
                                                                                                       gaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGaa                                               <  2:1476389/149‑1 (MQ=255)
                                                                                                         aaTACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAgg                                             <  2:879637/149‑1 (MQ=255)
                                                                                                          aTACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGga                                            >  2:719368/1‑149 (MQ=255)
                                                                                                             cAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGat                                         <  1:1481091/149‑1 (MQ=255)
                                                                                                              aaTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGata                                        >  1:181780/1‑149 (MQ=255)
                                                                                                              aaTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGata                                        >  1:593630/1‑149 (MQ=255)
                                                                                                               aTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGatat                                       <  1:581253/149‑1 (MQ=255)
                                                                                                               aTAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGatat                                       <  1:1148116/149‑1 (MQ=255)
                                                                                                                 aaGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATgg                                     <  2:1516317/149‑1 (MQ=255)
                                                                                                                   gCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGt                                                                  >  2:852799/1‑118 (MQ=255)
                                                                                                                   gCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGca                                   >  1:687118/1‑149 (MQ=255)
                                                                                                                     aGGAATTTATATTATCTCCCCCCGATTACCGTCATAGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac                                  <  2:662290/149‑1 (MQ=255)
                                                                                                                     aGGAATTTATATTATCTCCCCCGATGACCGTCATCGGAGGAGATATAACTGTAAGGAAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTTCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca                                 >  1:329052/1‑149 (MQ=255)
                                                                                                                      ggAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg                                >  1:1489450/1‑149 (MQ=255)
                                                                                                                       gAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc                               >  2:1074180/1‑149 (MQ=255)
                                                                                                                        aaTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa                              <  2:475197/149‑1 (MQ=255)
                                                                                                                         aTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc                             <  2:1487551/149‑1 (MQ=255)
                                                                                                                         aTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc                             <  1:998638/149‑1 (MQ=255)
                                                                                                                          tttATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt                            >  2:1292114/1‑149 (MQ=255)
                                                                                                                             atatTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa                         <  1:1368102/149‑1 (MQ=255)
                                                                                                                             atatTATCTCCCCCGATTACCGTCATCGGAGGACATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa                         <  1:793575/149‑1 (MQ=255)
                                                                                                                              ttatatCTCCTCCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag                        >  1:1473706/4‑149 (MQ=39)
                                                                                                                              ttatatCTCCTCCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag                        >  2:794205/4‑149 (MQ=39)
                                                                                                                              tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag                        <  1:1209439/149‑1 (MQ=255)
                                                                                                                              tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag                        <  2:529658/149‑1 (MQ=255)
                                                                                                                               attatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga                       <  1:476775/149‑1 (MQ=255)
                                                                                                                                ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag                      <  2:698547/149‑1 (MQ=255)
                                                                                                                                ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag                      <  2:1227510/149‑1 (MQ=255)
                                                                                                                                ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGCCACAGCACTAAagag                      >  1:1096464/1‑149 (MQ=255)
                                                                                                                                ttatCTCCCCCGATTACCGACATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag                      <  2:394246/149‑1 (MQ=255)
                                                                                                                                  atCTCCTCCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCTTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGc                    <  1:794205/149‑1 (MQ=255)
                                                                                                                                         cccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGTGCTGCGCAATTGATCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCAACAAt              >  1:36897/1‑149 (MQ=255)
                                                                                                                                           cGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATca            >  1:922455/1‑149 (MQ=255)
                                                                                                                                             aTTACCGTCATCGGAGGAGATATAACTGTCAGTCAAATATCGTCATAAAAAACGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATcacc          <  1:244072/149‑1 (MQ=255)
                                                                                                                                             aTTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATcacc          <  1:751701/149‑1 (MQ=255)
                                                                                                                                               tACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATcaccac        <  2:1087038/149‑1 (MQ=255)
                                                                                                                                                 ccGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCAccacca      <  1:730388/149‑1 (MQ=255)
                                                                                                                                                  cGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCAt     >  2:908146/1‑149 (MQ=255)
                                                                                                                                                     cATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTc  <  2:1560466/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAGGCCACCATCACCACCATTTC  >  NC_000913/1270581‑1270877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: