Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,522 T→G 6.4% intergenic (‑194/+16) tyrV ← / ← tyrT tRNA‑Tyr/tRNA‑Tyr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,5220TG 6.4% 265.3 / 9.0 94intergenic (‑194/+16)tyrV/tyrTtRNA‑Tyr/tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base T (58/30);  new base G (4/2);  total (62/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

GAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGC  >  NC_000913/1287374‑1287670
                                                                                                                                                    |                                                                                                                                                    
gAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACAt                                                                                                                                                      >  2:1536298/1‑149 (MQ=255)
 aaGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATc                                                                                                                                                     <  2:601062/149‑1 (MQ=255)
  aGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCg                                                                                                                                                    >  1:482904/1‑149 (MQ=255)
  aGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCg                                                                                                                                                    >  1:1579939/1‑149 (MQ=255)
  aGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCg                                                                                                                                                    <  2:384440/149‑1 (MQ=255)
         ttCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggt                                                                                                                                             >  1:389303/1‑149 (MQ=255)
         ttCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggt                                                                                                                                             <  1:429562/149‑1 (MQ=255)
          tcgtcgCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCGTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggtg                                                                                                                                            >  2:39721/1‑149 (MQ=255)
            gtcgCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGaa                                                                                                                                          <  2:560071/149‑1 (MQ=255)
            gtcgCTTCGCTCCTCACCCTTCGGGACGTTGCCTGTGGCAACGTTCTCTCGCTTTAGCTCGAATCTAACCTTAGTCGAATGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACAGCGATgtggtaac                                                                                                                                          <  2:126714/149‑4 (MQ=255)
             tcgcttcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAAt                                                                                                                                         <  1:835366/149‑1 (MQ=255)
                 ttcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtg                                                                                                                                     <  1:1559158/149‑1 (MQ=255)
                  tcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtgg                                                                                                                                    <  1:179773/149‑1 (MQ=255)
                    gctCCTCGCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtggtg                                                                                                                                  <  2:645461/149‑1 (MQ=255)
                      tcctcACCCTTCGGGCCGTTGCGTGTGGCAACGTTCTCTCGCTTTCGCTCGCATCGAACCTTAGTCGAAGGTTCTAACCCTTCCACGATGAGTGCAAACTTTCACACTCTGACCGAAGTTTCGACATGGCTGTGGTGTATTAtggttgt                                                                                                                                >  1:210433/1‑149 (MQ=255)
                         tcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTggg                                                                                                                             >  2:353214/1‑149 (MQ=255)
                           aCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTggggg                                                                                                                           >  2:134454/1‑149 (MQ=255)
                             ccTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGaa                                                                                                                         >  1:654960/1‑149 (MQ=255)
                             ccTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGGGCAATCTTTCACCATCTCCCCGAAGTTACCACATCGCTGTGGTGATGTATGGTGGTGGGGGaa                                                                                                                         >  1:819405/1‑149 (MQ=255)
                               ttCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTAACACATAGCTGTGGTGAATTATGGTGGTGGGGGAAgg                                                                                                                       >  2:1186799/1‑149 (MQ=255)
                                  gggCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGAtt                                                                                                                    >  2:192048/1‑149 (MQ=255)
                                   ggCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTc                                                                                                                   <  2:1268644/149‑1 (MQ=255)
                                     ccGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGa                                                                                                                 >  2:1372283/1‑149 (MQ=255)
                                      cGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGATGGTGGGGGAAGGATTCGaa                                                                                                                >  1:1114581/1‑149 (MQ=255)
                                      cGTTGCCTGTGGCAACGTTCTCTAGCTTTCGCTAGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTTAATTATGGTGGTGGGGGAAGGATTCGaa                                                                                                                <  2:1553523/149‑1 (MQ=255)
                                         tGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCTAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCt                                                                                                             <  2:334772/149‑1 (MQ=255)
                                            cTGTGGCAACGTTCTCTAGCTTTCGCTCGAATCGAACCTTAGTCGAATGTTATCACCCTTACCCGATGAGTGCAAACATTCACAATCTCACCGAAGTTACCCCATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTAGAAccatag                                                                                                          >  2:1439399/1‑145 (MQ=255)
                                             tgtgGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGa                                                                                                         >  1:390870/1‑149 (MQ=255)
                                               tgGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCACCGATGAGTGCAAACATTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGaat                                                                                                       >  2:1054200/1‑148 (MQ=255)
                                                 gCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCCCCCTTCCCCGATGAGTGCAAACTTTCACAATCTCGCCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGGGGGGGAAGGATTCGCACCTTCGAAGTc                                                                                                     >  1:163251/1‑149 (MQ=255)
                                                  cAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                                                                                                    >  2:1580998/1‑149 (MQ=255)
                                                  cAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGGCGATGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGCAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                                                                                                    <  1:1539113/149‑1 (MQ=255)
                                                    aCGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTGCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                                                                                                  <  1:655188/149‑1 (MQ=255)
                                                       ttCTCTCGCTTTCGCTCGAATCGATCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCTTACTTTCAGAATCTCAACGAAGTTACCACTTCGGTGTGGTGAATTATGGTGGTGGGGGTAGGATTGGAACCTTCGAAGTCGATGag                                                                                               >  1:295646/1‑148 (MQ=255)
                                                       ttCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGAc                                                                                               >  2:956986/1‑149 (MQ=255)
                                                       ttCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTAGAACCTTCGAAGTCTATGAc                                                                                               >  1:291041/1‑149 (MQ=255)
                                                           ctcGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACCTCGCGGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa                                                                                           >  1:302545/1‑149 (MQ=255)
                                                           ctcGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa                                                                                           <  1:1536298/149‑1 (MQ=255)
                                                            tcGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg                                                                                          >  2:534026/1‑149 (MQ=255)
                                                               cTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAtt                                                                                       <  2:877648/149‑1 (MQ=255)
                                                                tttCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACAGCGATgtggt                                                                                                                                             <  1:480007/94‑1 (MQ=255)
                                                                tttCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACAGCGATgtggt                                                                                                                                             >  2:480007/1‑94 (MQ=255)
                                                                    gCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg                                                                                  >  1:983727/1‑149 (MQ=255)
                                                                     cTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt                                                                                 >  1:218393/1‑149 (MQ=255)
                                                                      tcgaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                                                                                >  1:727061/1‑149 (MQ=255)
                                                                      tcgaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                                                                                >  2:685186/1‑149 (MQ=255)
                                                                      tcgaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                                                                                <  2:218393/149‑1 (MQ=255)
                                                                      tcgaatcgaaCCTTAGTCGAAGGTTCTCACCCTTACCCGATGAGTGCAAACTTTAACAATATCACCGAAGTTACCACATAGATTTGGTTAATTATGGTGGTGGGGGAAGGATTCTAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                                                                                >  2:1167208/1‑149 (MQ=37)
                                                                        gaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                                                              >  1:1355340/1‑149 (MQ=255)
                                                                        gaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATACGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                                                              >  2:386288/1‑149 (MQ=255)
                                                                         aatcgaaCCTTAGTCGAAGGTTATAACCCATACCCGAAGAGTTCAAACTTTAACAAAATCACAGAATTTACCACATCGCAGTGGTTAATTATGGTGGTGGGGGAATGATTCGAACCTTAGAATTCGATTACGGCAGATTTAAAGTCTGc                                                                             >  2:1224553/1‑149 (MQ=255)
                                                                          atcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCt                                                                            >  2:791168/1‑149 (MQ=255)
                                                                                 cTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTg                                                                     >  2:1373639/1‑149 (MQ=255)
                                                                                  ttAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTgg                                                                    >  1:527376/1‑149 (MQ=255)
                                                                                   tAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGc                                                                   >  1:1557233/1‑149 (MQ=255)
                                                                                      tCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCCCCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGc                                                                >  2:808416/1‑149 (MQ=255)
                                                                                      tCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGACGATGACGGCAGATTTACAGTCTGCTCCCGTTGGCCGc                                                                >  2:710043/1‑149 (MQ=255)
                                                                                        gAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTc                                                              >  1:1470816/1‑149 (MQ=255)
                                                                                           ggTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                                                           >  2:1163900/1‑149 (MQ=255)
                                                                                           ggTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATCGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTCCTCCCTTTGGCCGCTCggg                                                           >  2:995204/1‑149 (MQ=255)
                                                                                               ctcACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAcc                                                       >  1:371274/1‑149 (MQ=255)
                                                                                                  aCCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                                                              >  2:662336/1‑123 (MQ=255)
                                                                                                  aCCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                                                              <  1:662336/123‑1 (MQ=255)
                                                                                                     cTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCccacca                                                 >  2:1281303/1‑149 (MQ=255)
                                                                                                          ccGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACgggg                                            >  2:1240007/1‑149 (MQ=255)
                                                                                                           cGATGAGTGCAAACTTTCACGATCTCACCGAACTTACCACATCGCTGTTGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGGCGATGACGGCAGAATTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGt                                           <  1:1256518/149‑1 (MQ=21)
                                                                                                           cGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGt                                           >  1:1123631/1‑149 (MQ=255)
                                                                                                           cGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGt                                           <  2:1557233/149‑1 (MQ=255)
                                                                                                                 gTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTTGCCGCTCGGGAACCCCACCACGGGGTAATGCt                                     <  1:1580998/149‑1 (MQ=255)
                                                                                                                 gTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACAGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACAACGGGGTAATGCt                                     >  2:1133819/1‑149 (MQ=255)
                                                                                                                 gTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGATGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCt                                     <  2:1114581/149‑1 (MQ=255)
                                                                                                                  tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCCCGGGGTAATGCtt                                    >  2:781606/1‑149 (MQ=255)
                                                                                                                  tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCtt                                    >  1:911826/1‑149 (MQ=255)
                                                                                                                  tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCtt                                    >  2:856680/1‑149 (MQ=255)
                                                                                                                   gCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCttt                                   <  1:875174/149‑1 (MQ=255)
                                                                                                                    cAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCtttt                                  >  1:463343/1‑149 (MQ=255)
                                                                                                                    cAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCtttt                                  >  1:398391/1‑149 (MQ=255)
                                                                                                                      aaCTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTa                                <  2:389303/149‑1 (MQ=255)
                                                                                                                        cTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACt                              <  2:1579939/149‑1 (MQ=255)
                                                                                                                         tttCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTg                             >  1:428185/1‑149 (MQ=255)
                                                                                                                         tttAACAATCTCACCGAAGTTACGACCTCGCCGTCGTGAATTATGGTGGTGGGGGAAGGATTCGAACCATCGAAGTCGATGACGGCTGATTTACAGTCTGCTCCATTTGGCCGCTCGGGAACCCCACCTCGGGGTAATGCTTTTTACTg                             <  1:1054200/149‑1 (MQ=18)
                                                                                                                           tCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGc                           <  2:654960/149‑1 (MQ=255)
                                                                                                                           tCACAATCTCACCGAAGTTACCACATCGCGGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCACTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGc                           >  1:1382138/1‑149 (MQ=255)
                                                                                                                                 tctcACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCCCCACGGCGAAATGCTTATAACTGGCCTGCTc                     >  2:487666/1‑149 (MQ=21)
                                                                                                                                 tctcACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTc                     >  2:487664/1‑149 (MQ=255)
                                                                                                                                   tcACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTccc                   <  2:1123631/149‑1 (MQ=255)
                                                                                                                                   tcACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTccc                   <  1:685186/149‑1 (MQ=255)
                                                                                                                                     aCCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCtt                 >  1:1408334/1‑149 (MQ=255)
                                                                                                                                       cGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACGCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTAt               <  2:983727/149‑1 (MQ=255)
                                                                                                                                           ttcaCCACAGCGATGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCggg           >  2:661195/4‑149 (MQ=255)
                                                                                                                                           ttcaCCACAGCGATGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCggg           >  2:1479976/4‑149 (MQ=255)
                                                                                                                                            ttACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGa          >  2:548250/1‑149 (MQ=255)
                                                                                                                                              aCCACAGCGATGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCACTTATCGGGAAg        >  2:772682/1‑149 (MQ=255)
                                                                                                                                                 acaTCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCgg     <  1:856680/149‑1 (MQ=255)
                                                                                                                                                   aTCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCCCCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCgggg   >  1:290497/1‑149 (MQ=255)
                                                                                                                                                    tCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGgc  >  1:1483070/1‑149 (MQ=255)
                                                                                                                                                    tCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGgc  <  2:734657/149‑1 (MQ=255)
                                                                                                                                                    tCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGgc  <  1:534026/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGC  >  NC_000913/1287374‑1287670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑