Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,287,525	C→A	7.0%	intergenic (‑197/+13)	tyrV ← / ← tyrT	tRNA‑Tyr/tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,525	0	C	A	7.0%	260.6 / 8.3	94	intergenic (‑197/+13)	tyrV/tyrT	tRNA‑Tyr/tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (55/31);  new base A (5/2);  total (61/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

TTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGC  >  NC_000913/1287383‑1287672                                                                                                                                               |                                                                                                                                                    ttCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggt                                                                                                                                               >  1:389303/1‑149 (MQ=255) ttCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggt                                                                                                                                               <  1:429562/149‑1 (MQ=255)  tcgtcgCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCGTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggtg                                                                                                                                              >  2:39721/1‑149 (MQ=255)    gtcgCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGaa                                                                                                                                            <  2:560071/149‑1 (MQ=255)    gtcgCTTCGCTCCTCACCCTTCGGGACGTTGCCTGTGGCAACGTTCTCTCGCTTTAGCTCGAATCTAACCTTAGTCGAATGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACAGCGATgtggtaac                                                                                                                                            <  2:126714/149‑4 (MQ=255)     tcgcttcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAAt                                                                                                                                           <  1:835366/149‑1 (MQ=255)         ttcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtg                                                                                                                                       <  1:1559158/149‑1 (MQ=255)          tcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtgg                                                                                                                                      <  1:179773/149‑1 (MQ=255)            gctCCTCGCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtggtg                                                                                                                                    <  2:645461/149‑1 (MQ=255)              tcctcACCCTTCGGGCCGTTGCGTGTGGCAACGTTCTCTCGCTTTCGCTCGCATCGAACCTTAGTCGAAGGTTCTAACCCTTCCACGATGAGTGCAAACTTTCACACTCTGACCGAAGTTTCGACATGGCTGTGGTGTATTAtggttgt                                                                                                                                  >  1:210433/1‑149 (MQ=255)                 tcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTggg                                                                                                                               >  2:353214/1‑149 (MQ=255)                   aCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTggggg                                                                                                                             >  2:134454/1‑149 (MQ=255)                     ccTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGaa                                                                                                                           >  1:654960/1‑149 (MQ=255)                     ccTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGGGCAATCTTTCACCATCTCCCCGAAGTTACCACATCGCTGTGGTGATGTATGGTGGTGGGGGaa                                                                                                                           >  1:819405/1‑149 (MQ=255)                       ttCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTAACACATAGCTGTGGTGAATTATGGTGGTGGGGGAAgg                                                                                                                         >  2:1186799/1‑149 (MQ=255)                          gggCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGAtt                                                                                                                      >  2:192048/1‑149 (MQ=255)                           ggCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTc                                                                                                                     <  2:1268644/149‑1 (MQ=255)                             ccGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGa                                                                                                                   >  2:1372283/1‑149 (MQ=255)                              cGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGATGGTGGGGGAAGGATTCGaa                                                                                                                  >  1:1114581/1‑149 (MQ=255)                              cGTTGCCTGTGGCAACGTTCTCTAGCTTTCGCTAGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTTAATTATGGTGGTGGGGGAAGGATTCGaa                                                                                                                  <  2:1553523/149‑1 (MQ=255)                                 tGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCTAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCt                                                                                                               <  2:334772/149‑1 (MQ=255)                                    cTGTGGCAACGTTCTCTAGCTTTCGCTCGAATCGAACCTTAGTCGAATGTTATCACCCTTACCCGATGAGTGCAAACATTCACAATCTCACCGAAGTTACCCCATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTAGAAccatag                                                                                                            >  2:1439399/1‑145 (MQ=255)                                     tgtgGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGa                                                                                                           >  1:390870/1‑149 (MQ=255)                                       tgGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCACCGATGAGTGCAAACATTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGaat                                                                                                         >  2:1054200/1‑148 (MQ=255)                                         gCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCCCCCTTCCCCGATGAGTGCAAACTTTCACAATCTCGCCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGGGGGGGAAGGATTCGCACCTTCGAAGTc                                                                                                       >  1:163251/1‑149 (MQ=255)                                          cAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                                                                                                      >  2:1580998/1‑149 (MQ=255)                                          cAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGGCGATGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGCAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                                                                                                      <  1:1539113/149‑1 (MQ=255)                                            aCGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTGCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                                                                                                    <  1:655188/149‑1 (MQ=255)                                               ttCTCTCGCTTTCGCTCGAATCGATCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCTTACTTTCAGAATCTCAACGAAGTTACCACTTCGGTGTGGTGAATTATGGTGGTGGGGGTAGGATTGGAACCTTCGAAGTCGATGag                                                                                                 >  1:295646/1‑148 (MQ=255)                                               ttCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGAc                                                                                                 >  2:956986/1‑149 (MQ=255)                                               ttCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTAGAACCTTCGAAGTCTATGAc                                                                                                 >  1:291041/1‑149 (MQ=255)                                                   ctcGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACCTCGCGGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa                                                                                             >  1:302545/1‑149 (MQ=255)                                                   ctcGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa                                                                                             <  1:1536298/149‑1 (MQ=255)                                                    tcGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg                                                                                            >  2:534026/1‑149 (MQ=255)                                                       cTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAtt                                                                                         <  2:877648/149‑1 (MQ=255)                                                        tttCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACAGCGATgtggt                                                                                                                                               <  1:480007/94‑1 (MQ=255)                                                        tttCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACAGCGATgtggt                                                                                                                                               >  2:480007/1‑94 (MQ=255)                                                            gCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg                                                                                    >  1:983727/1‑149 (MQ=255)                                                             cTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt                                                                                   >  1:218393/1‑149 (MQ=255)                                                              tcgaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                                                                                  <  2:218393/149‑1 (MQ=255)                                                              tcgaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                                                                                  >  1:727061/1‑149 (MQ=255)                                                              tcgaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                                                                                  >  2:685186/1‑149 (MQ=255)                                                              tcgaatcgaaCCTTAGTCGAAGGTTCTCACCCTTACCCGATGAGTGCAAACTTTAACAATATCACCGAAGTTACCACATAGATTTGGTTAATTATGGTGGTGGGGGAAGGATTCTAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                                                                                  >  2:1167208/1‑149 (MQ=37)                                                                gaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                                                                >  1:1355340/1‑149 (MQ=255)                                                                gaatcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATACGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                                                                >  2:386288/1‑149 (MQ=255)                                                                 aatcgaaCCTTAGTCGAAGGTTATAACCCATACCCGAAGAGTTCAAACTTTAACAAAATCACAGAATTTACCACATCGCAGTGGTTAATTATGGTGGTGGGGGAATGATTCGAACCTTAGAATTCGATTACGGCAGATTTAAAGTCTGc                                                                               >  2:1224553/1‑149 (MQ=255)                                                                  atcgaaCCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCt                                                                              >  2:791168/1‑149 (MQ=255)                                                                         cTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTg                                                                       >  2:1373639/1‑149 (MQ=255)                                                                          ttAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTgg                                                                      >  1:527376/1‑149 (MQ=255)                                                                           tAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGc                                                                     >  1:1557233/1‑149 (MQ=255)                                                                              tCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCCCCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGc                                                                  >  2:808416/1‑149 (MQ=255)                                                                              tCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGACGATGACGGCAGATTTACAGTCTGCTCCCGTTGGCCGc                                                                  >  2:710043/1‑149 (MQ=255)                                                                                gAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTc                                                                >  1:1470816/1‑149 (MQ=255)                                                                                   ggTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg                                                             >  2:1163900/1‑149 (MQ=255)                                                                                   ggTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATCGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTCCTCCCTTTGGCCGCTCggg                                                             >  2:995204/1‑149 (MQ=255)                                                                                       ctcACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAcc                                                         >  1:371274/1‑149 (MQ=255)                                                                                          aCCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                                                                >  2:662336/1‑123 (MQ=255)                                                                                          aCCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                                                                                <  1:662336/123‑1 (MQ=255)                                                                                             cTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCccacca                                                   >  2:1281303/1‑149 (MQ=255)                                                                                                  ccGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACgggg                                              >  2:1240007/1‑149 (MQ=255)                                                                                                   cGATGAGTGCAAACTTTCACGATCTCACCGAACTTACCACATCGCTGTTGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGGCGATGACGGCAGAATTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGt                                             <  1:1256518/149‑1 (MQ=21)                                                                                                   cGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGt                                             >  1:1123631/1‑149 (MQ=255)                                                                                                   cGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGt                                             <  2:1557233/149‑1 (MQ=255)                                                                                                         gTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTTGCCGCTCGGGAACCCCACCACGGGGTAATGCt                                       <  1:1580998/149‑1 (MQ=255)                                                                                                         gTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACAGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACAACGGGGTAATGCt                                       >  2:1133819/1‑149 (MQ=255)                                                                                                         gTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGATGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCt                                       <  2:1114581/149‑1 (MQ=255)                                                                                                          tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCCCGGGGTAATGCtt                                      >  2:781606/1‑149 (MQ=255)                                                                                                          tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCtt                                      >  1:911826/1‑149 (MQ=255)                                                                                                          tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCtt                                      >  2:856680/1‑149 (MQ=255)                                                                                                           gCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCttt                                     <  1:875174/149‑1 (MQ=255)                                                                                                            cAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCtttt                                    >  1:463343/1‑149 (MQ=255)                                                                                                            cAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCtttt                                    >  1:398391/1‑149 (MQ=255)                                                                                                              aaCTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTa                                  <  2:389303/149‑1 (MQ=255)                                                                                                                cTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACt                                <  2:1579939/149‑1 (MQ=255)                                                                                                                 tttCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTg                               >  1:428185/1‑149 (MQ=255)                                                                                                                 tttAACAATCTCACCGAAGTTACGACCTCGCCGTCGTGAATTATGGTGGTGGGGGAAGGATTCGAACCATCGAAGTCGATGACGGCTGATTTACAGTCTGCTCCATTTGGCCGCTCGGGAACCCCACCTCGGGGTAATGCTTTTTACTg                               <  1:1054200/149‑1 (MQ=18)                                                                                                                   tCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGc                             <  2:654960/149‑1 (MQ=255)                                                                                                                   tCACAATCTCACCGAAGTTACCACATCGCGGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCACTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGc                             >  1:1382138/1‑149 (MQ=255)                                                                                                                         tctcACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCCCCACGGCGAAATGCTTATAACTGGCCTGCTc                       >  2:487666/1‑149 (MQ=21)                                                                                                                         tctcACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTc                       >  2:487664/1‑149 (MQ=255)                                                                                                                           tcACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTccc                     <  2:1123631/149‑1 (MQ=255)                                                                                                                           tcACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTccc                     <  1:685186/149‑1 (MQ=255)                                                                                                                             aCCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCtt                   >  1:1408334/1‑149 (MQ=255)                                                                                                                               cGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACGCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTAt                 <  2:983727/149‑1 (MQ=255)                                                                                                                                   ttcaCCACAGCGATGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCggg             >  2:661195/4‑149 (MQ=255)                                                                                                                                   ttcaCCACAGCGATGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCggg             >  2:1479976/4‑149 (MQ=255)                                                                                                                                    ttACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGa            >  2:548250/1‑149 (MQ=255)                                                                                                                                      aCCACAGCGATGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCACTTATCGGGAAg          >  2:772682/1‑149 (MQ=255)                                                                                                                                         acaTCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCgg       <  1:856680/149‑1 (MQ=255)                                                                                                                                           aTCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCCCCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCgggg     >  1:290497/1‑149 (MQ=255)                                                                                                                                            tCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGgc    <  1:534026/149‑1 (MQ=255)                                                                                                                                            tCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGgc    <  2:734657/149‑1 (MQ=255)                                                                                                                                            tCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGgc    >  1:1483070/1‑149 (MQ=255)                                                                                                                                              gCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGgcgc  <  1:134454/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    TTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGC  >  NC_000913/1287383‑1287672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑