Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,315,807 C→T 5.3% intergenic (‑37/+49) yciF ← / ← yciG putative rubrerythrin/ferritin‑like metal‑binding protein/KGG family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,315,8070CT 5.3% 361.0 / 5.2 112intergenic (‑37/+49)yciF/yciGputative rubrerythrin/ferritin‑like metal‑binding protein/KGG family protein
Reads supporting (aligned to +/- strand):  ref base C (51/55);  new base T (4/2);  total (55/57)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 117 total aligned reads displayed.

CATTTGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATG  >  NC_000913/1315659‑1315954
                                                                                                                                                    |                                                                                                                                                   
cATTTGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGcc                                                                                                                                                     <  2:952599/149‑1 (MQ=255)
 aTTTGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGccc                                                                                                                                                    <  1:1015821/149‑1 (MQ=255)
 aTTTGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGccc                                                                                                                                                    <  1:578421/149‑1 (MQ=255)
  tttGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCg                                                                                                                                                   >  1:443528/1‑149 (MQ=255)
   ttGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGc                                                                                                                                                  >  2:1259891/1‑149 (MQ=255)
    tGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCa                                                                                                                                                 <  2:1203555/149‑1 (MQ=255)
     taTGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGGGCCGTATGGCTCGCAg                                                                                                                                                <  2:1392587/148‑1 (MQ=255)
     gATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAg                                                                                                                                                >  1:1275361/1‑149 (MQ=255)
       tGTTTCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTTTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGAt                                                                                                                                              <  1:1020754/149‑1 (MQ=255)
       tGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGAt                                                                                                                                              <  1:1570330/149‑1 (MQ=255)
           gCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCaa                                                                                                                                          <  1:428112/149‑1 (MQ=255)
                  ggAGTTTTGGCATTGCGCGTGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTTAATAAATACATCTTCAATGGTCTTCATATTCATTTTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCtt                                                                                                                                   <  1:1479267/148‑1 (MQ=255)
                  cGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCtt                                                                                                                                   <  2:456130/149‑1 (MQ=255)
                  cGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCtt                                                                                                                                   <  2:1283421/149‑1 (MQ=255)
                     tttttGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTTAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTAt                                                                                                                                <  2:1198221/148‑1 (MQ=255)
                          gCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCt                                                                                                                           <  1:300236/149‑1 (MQ=255)
                          gCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCt                                                                                                                           <  1:1232393/149‑1 (MQ=255)
                           ccAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGGGCCGTATGGCTCGCAgtgattt                                                                                                                                          <  1:193245/133‑7 (MQ=255)
                           ccAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGGGCCGTATGGCTCGCAgtgattt                                                                                                                                          >  2:193245/1‑127 (MQ=255)
                            cAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGa                                                                                                                         <  1:643215/149‑1 (MQ=255)
                            cAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCAATACGGCCCGCAGATGCAATTATCTTTATCAGCTGa                                                                                                                         >  1:102939/1‑149 (MQ=255)
                              gttaccGGTTTAATTGTTTTTCTGAGCTGTTGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTAATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCATCTGata                                                                                                                       <  1:79122/145‑1 (MQ=255)
                               tGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCATGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGatat                                                                                                                      <  2:640745/149‑1 (MQ=255)
                                 cccGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATta                                                                                                                    >  1:1375521/1‑149 (MQ=255)
                                  ccGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATtaa                                                                                                                   <  2:1375521/149‑1 (MQ=255)
                                        tAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTa                                                                                                             >  2:489702/1‑149 (MQ=255)
                                         aaTTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTaa                                                                                                            <  2:595162/149‑1 (MQ=255)
                                          aTTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATACATATTTTTCTCCAGAGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAAc                                                                                                           <  2:1475846/149‑1 (MQ=255)
                                           ttGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACg                                                                                                          >  2:1342577/1‑149 (MQ=255)
                                              ttattCTGCGCTGTAGGAATCTGAAAGCAGGTGAATTAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTCCCAGCCATACGGCCCGCAGATGCAAATATCTTTATAAGCTGATATTAATAATTAAAGGTg                                                                                                       >  2:663806/4‑149 (MQ=255)
                                                    tGCGCTGTAGGTATCTGAAAGCAGGTGAAAAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCAGAAATTAATAATTAACGGTGAATAAg                                                                                                 >  2:657642/1‑149 (MQ=255)
                                                        cTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACTGTGAATCAGGAtt                                                                                             >  1:1008615/1‑149 (MQ=255)
                                                        cTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGAtt                                                                                             >  2:271715/1‑149 (MQ=255)
                                                         tGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGAttt                                                                                            >  2:398159/1‑149 (MQ=255)
                                                         tGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGAttt                                                                                            <  1:1057562/149‑1 (MQ=255)
                                                         tGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATATTTATCAGCTGATATTAATAATTAACGGTGAATCAGGAtat                                                                                            >  1:502393/1‑147 (MQ=255)
                                                          gTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTg                                                                                           <  2:1275361/149‑1 (MQ=255)
                                                           tatgTATCTGAAAGCATTTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGc                                                                                          <  1:837779/146‑1 (MQ=255)
                                                                aTCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGAt                                                                                     >  1:543163/1‑149 (MQ=255)
                                                                aTCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGAt                                                                                     >  2:368709/1‑149 (MQ=255)
                                                                aTCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCAGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCATGAt                                                                                     >  1:1084347/1‑149 (MQ=255)
                                                                 tCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGAtt                                                                                    <  1:271715/149‑1 (MQ=255)
                                                                  cTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGAttt                                                                                   <  1:769763/149‑1 (MQ=255)
                                                                   tGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATtta                                                                                  >  2:699598/1‑149 (MQ=255)
                                                                   tGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATtta                                                                                  >  2:563220/1‑149 (MQ=255)
                                                                   tGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATtta                                                                                  <  2:166853/149‑1 (MQ=255)
                                                                   tGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACAGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATtta                                                                                  >  2:863525/1‑149 (MQ=255)
                                                                    tatagaaGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATttat                                                                                 <  1:1236629/143‑1 (MQ=255)
                                                                    gAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACTGCCCGCAGATGCAATTATCTTTATCAGAAGATATTAATAATAAACGGAGAATCAGGATTTGCCTGATttat                                                                                 >  1:52844/1‑149 (MQ=255)
                                                                    gAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATttat                                                                                 >  2:1538500/1‑149 (MQ=255)
                                                                     aaaGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATttatt                                                                                <  1:1464530/149‑1 (MQ=255)
                                                                     aaaGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATttatt                                                                                <  2:1361133/149‑1 (MQ=255)
                                                                     aaaGCAGGCGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATttatt                                                                                <  1:846660/149‑1 (MQ=255)
                                                                      aaGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATttatta                                                                               <  2:1461558/149‑1 (MQ=255)
                                                                        gCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTacc                                                                             >  2:937871/1‑149 (MQ=255)
                                                                            gTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCg                                                                         >  2:1215187/1‑149 (MQ=255)
                                                                                 taaataCATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTttg                                                                    <  1:1342577/149‑1 (MQ=255)
                                                                                   aataCATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTttgtt                                                                  <  2:1330731/149‑1 (MQ=255)
                                                                                        aTCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCg                                                             >  2:1476491/1‑149 (MQ=255)
                                                                                          cTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGcc                                                           >  2:523199/1‑149 (MQ=255)
                                                                                           ttCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCt                                                          >  1:581470/1‑149 (MQ=255)
                                                                                           ttCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAAGAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGACt                                                          >  2:131744/1‑149 (MQ=255)
                                                                                              aaTGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGCTGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCACGAATTGCCTGATTTATTACCACCGCTTTGTTGCCCGCCttat                                                       >  2:1136687/1‑147 (MQ=255)
                                                                                              aaTGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCtttt                                                       <  1:220721/149‑1 (MQ=255)
                                                                                               aTGGTCATCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCttttt                                                      <  2:340827/149‑1 (MQ=255)
                                                                                                 ggTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTa                                                    <  1:1258035/149‑1 (MQ=255)
                                                                                                  gTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTAc                                                   <  2:1172114/149‑1 (MQ=255)
                                                                                                    cTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTAccc                                                 >  1:446039/1‑149 (MQ=255)
                                                                                                     ttcatattcataTTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCg                                                >  1:1450916/1‑149 (MQ=255)
                                                                                                        atattcatattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGGTATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCtt                                             >  1:354788/1‑149 (MQ=255)
                                                                                                        atattcatattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCtt                                             >  1:681253/1‑149 (MQ=255)
                                                                                                              atattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTAc                                                   >  1:900544/1‑137 (MQ=255)
                                                                                                              atattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTAc                                                   <  2:900544/137‑1 (MQ=255)
                                                                                                               tattTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATgc                                      >  1:1121102/1‑149 (MQ=255)
                                                                                                                 tttttCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATgcgc                                    <  2:13438/149‑1 (MQ=255)
                                                                                                                         cAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGa                            <  1:1596844/149‑1 (MQ=255)
                                                                                                                           gTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATc                          >  1:837725/1‑149 (MQ=255)
                                                                                                                           gTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCATGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATc                          >  2:154693/1‑149 (MQ=255)
                                                                                                                            tGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCa                         <  1:1500412/149‑1 (MQ=255)
                                                                                                                             gAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAt                        <  2:338109/149‑1 (MQ=255)
                                                                                                                              aaaTCTCTTCGAGCGATACGGCCCGGAGATGCAATTATCTTTATCTTCTGAGATTAATAATTAACGGGGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAtt                       <  2:258936/149‑1 (MQ=255)
                                                                                                                              aaaTCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAtt                       >  2:475933/1‑149 (MQ=255)
                                                                                                                               tgcatcTGCGGGCCGTATGGCTCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCGTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAttt                      >  1:157109/6‑149 (MQ=255)
                                                                                                                               tgcatcTGCGGGCCGTATGGCTCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAttt                      >  1:1186778/6‑149 (MQ=255)
                                                                                                                               aaTCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAttt                      <  2:197251/149‑1 (MQ=255)
                                                                                                                                aTCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACAACCGCTTTGTTGACCGCATTTTTTACCCGCTACAGATGCGCGTTGCGGATCAtttt                     >  1:175902/1‑149 (MQ=255)
                                                                                                                                 catcTGCGGGCCGTATGGCTCGCAGCTGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGACTCAGGATTTGCCTGATTTATTACCACAGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCAttttt                    >  1:74367/4‑149 (MQ=255)
                                                                                                                                  cACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTa                   <  2:1539247/149‑1 (MQ=255)
                                                                                                                                    cTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTaaa                 <  2:102939/149‑1 (MQ=255)
                                                                                                                                       cGAGCCATTCGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGGTTTATTACCACCGCGTTGTTGACCGCCTTTTTTAACCGCTTCAGATGCGCGTTGCGGATCATTTTTAACAtt              >  1:1423515/1‑149 (MQ=255)
                                                                                                                                       cGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAAtt              >  1:768241/1‑149 (MQ=255)
                                                                                                                                         aGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAc            <  1:523199/149‑1 (MQ=255)
                                                                                                                                          gCCATCCGGCCCGCAGATGCAAATATCTATAGCAGCAGAAATTAATAAAAAACGGTGAATCAGGATTAGCCTGATTTATTACCACCGCGTTGTTGACCGCCCTTTTTACCCGCTTCAGATGCGCGTGGCGGATCATTTTTAAAATTAcc           <  2:1000585/149‑1 (MQ=255)
                                                                                                                                          gCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTACCGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAcc           <  2:721650/149‑1 (MQ=255)
                                                                                                                                           ccATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAccg          >  1:865261/1‑149 (MQ=255)
                                                                                                                                            cATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTAccgc         >  2:331861/1‑149 (MQ=255)
                                                                                                                                               aCGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACcgccgc      <  1:699598/149‑1 (MQ=255)
                                                                                                                                                  gCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTAt   <  1:154693/149‑1 (MQ=255)
                                                                                                                                                   cccGCAGATGCAATTATCTTTATCAGCTGATATTTATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACAACCGCTTTGTTGACCGACTTTTTTACCCGCTTCAGATGCGCGTTGAGTATCATTTTTAAAATTACCTCCGCTATg  >  2:263417/1‑149 (MQ=255)
                                                                                                                                                   cccGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATg  <  1:1594299/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CATTTGATGTTGCTCTTGCGAGTTTTGCCAGTGCCCGGGTTAATTGTTTTTCTGCGCTGTAGGTATCTGAAAGCAGGTGAATAAATACATCTTCAATGGTCTTCATATTCATATTTTTCTCCAGTGAAATCACTGCGAGCCATACGGCCCGCAGATGCAATTATCTTTATCAGCTGATATTAATAATTAACGGTGAATCAGGATTTGCCTGATTTATTACCACCGCTTTGTTGACCGCCTTTTTTACCCGCTTCAGATGCGCGTTGCGGATCATTTTTAAAATTACCGCCGCTATG  >  NC_000913/1315659‑1315954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: