Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,457,983 A→T 9.1% I163F (ATC→TTC)  paaF → 2,3‑dehydroadipyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,457,9830AT 9.1% 248.6 / 12.1 87I163F (ATC→TTC) paaF2,3‑dehydroadipyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (37/42);  new base T (5/3);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

TGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGC  >  NC_000913/1457836‑1458126
                                                                                                                                                   |                                                                                                                                                
tgCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTAt                                                                                                                                                 >  1:943144/1‑149 (MQ=255)
         gtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGTGATACTTTCTCCGCTCa                                                                                                                                        <  2:1258932/149‑1 (MQ=255)
         gtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCa                                                                                                                                        <  1:557617/149‑1 (MQ=255)
         gtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCa                                                                                                                                        <  1:515902/149‑1 (MQ=255)
         gtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCa                                                                                                                                        <  1:1349414/149‑1 (MQ=255)
         gtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCa                                                                                                                                        >  2:1085268/1‑149 (MQ=255)
          tggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGTGATACTTTCTCCGCTCag                                                                                                                                       <  2:1395149/149‑1 (MQ=255)
             gtGCCGGAGAGAACGCGCGTGTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTAGCACCGCTCagcaa                                                                                                                                    <  1:1501636/148‑1 (MQ=255)
                    agagaACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCAcagc                                                                                                                             <  2:900520/149‑1 (MQ=255)
                           gcgcgTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCgg                                                                                                                      >  1:264632/1‑149 (MQ=255)
                           gcgcgTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCgg                                                                                                                      >  1:1438804/1‑149 (MQ=255)
                            cgcgTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCagcaa                                                                                                                                    >  1:972929/1‑134 (MQ=255)
                            agcgTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCagcaa                                                                                                                                    <  2:972929/133‑1 (MQ=255)
                               gTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTg                                                                                                                  <  2:699938/149‑1 (MQ=255)
                                     ggTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGc                                                                                                            >  2:999255/1‑149 (MQ=255)
                                     ggTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGc                                                                                                            >  2:478438/1‑149 (MQ=255)
                                     ggTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGc                                                                                                            >  2:878435/1‑149 (MQ=255)
                                      gTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCg                                                                                                           >  2:1162130/1‑149 (MQ=255)
                                        tGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGAc                                                                                                         <  1:782739/149‑1 (MQ=255)
                                        tGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGAc                                                                                                         <  1:1388959/149‑1 (MQ=255)
                                        ggCCGGAAATCACTCTCGTCATCACGCCCGGCGCAGGCGGAACGCATCGTTTAATGCGTCGTGTCGGTAAATCGTTAGCCAGCAAACTGGTGCTGAGCGGAGAATGTTTCAACGCTCAGCAAGCTGAGCAGGCCGGGCTGTTTAGCGAc                                                                                                         <  1:314083/148‑1 (MQ=255)
                                          ccGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGt                                                                                                       >  2:590691/1‑149 (MQ=255)
                                              aactCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGGTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAACTGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑c                                                                                                  <  1:129748/146‑1 (MQ=255)
                                              aaaTCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑c                                                                                                  <  2:1566490/149‑1 (MQ=255)
                                              aaaTCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑c                                                                                                  <  1:1397353/149‑1 (MQ=255)
                                                atctgtctcTGCATCATGCGAGGCGCAGGCGGAACGCAACGCTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAATTGGTGCTGAGCGGATATAGTATCACCGCTGAGCAAGCACAGCAGTCATGGCTGGTTATCGACGTCTT‑ccc                                                                                                <  2:1280392/144‑1 (MQ=255)
                                                 tCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑cccc                                                                                               <  1:1523710/149‑1 (MQ=255)
                                                   aCTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAg                                                                                             >  2:230111/1‑149 (MQ=255)
                                                    ctctCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGc                                                                                            >  2:1150194/1‑149 (MQ=255)
                                                       tcGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGAt                                                                                         <  1:675922/149‑1 (MQ=255)
                                                           catcatGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTaa                                                                                     >  1:1568659/1‑149 (MQ=255)
                                                               atGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCt                                                                                 >  1:669724/1‑149 (MQ=255)
                                                                tGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTc                                                                                <  1:1115770/149‑1 (MQ=255)
                                                                tGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTc                                                                                <  1:544562/149‑1 (MQ=255)
                                                                tGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGGTAGCGACGTCTT‑CCCCAGCGATTTAACCCTc                                                                                <  2:261057/149‑1 (MQ=255)
                                                                tGCCTGGCGCAGGCGGAACGAAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTc                                                                                <  1:1388498/149‑1 (MQ=255)
                                                                tGCCTGGCGCAAGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTc                                                                                <  1:1430702/149‑1 (MQ=255)
                                                                  ccTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCAT‑CCCCAGCGATTTAACCCTCGa                                                                              >  2:1098773/1‑149 (MQ=255)
                                                                  ccTGGCGCAGGCGGAACGCAAAGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGGACAGCAGGACGGGCTGGTGAGCGACGTCTG‑CCCCAGCGCTTTAACCCTcta                                                                              >  2:852501/1‑147 (MQ=255)
                                                                   cTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGaa                                                                             <  1:1508900/149‑1 (MQ=255)
                                                                    tGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAAt                                                                            <  1:1579373/149‑1 (MQ=255)
                                                                     ggCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCGGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATa                                                                           >  2:543381/1‑149 (MQ=255)
                                                                     ggCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCt                                                                                 >  1:80120/1‑143 (MQ=255)
                                                                     ggCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCt                                                                                 <  2:80120/143‑1 (MQ=255)
                                                                       cgcAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACg                                                                         <  1:878435/149‑1 (MQ=255)
                                                                       cgcAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACg                                                                         <  1:478438/149‑1 (MQ=255)
                                                                       cgcAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACg                                                                         <  1:999255/149‑1 (MQ=255)
                                                                        gcAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGc                                                                        <  1:145860/149‑1 (MQ=255)
                                                                            gCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCTGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTa                                                                    <  1:543381/149‑1 (MQ=255)
                                                                            gCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGAAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTa                                                                    >  1:90545/1‑149 (MQ=255)
                                                                             cGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCCAGCGATTTAACCCTCGAATACGCCTTa                                                                    <  2:943144/149‑1 (MQ=255)
                                                                              ggAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACa                                                                  >  1:1571709/1‑149 (MQ=255)
                                                                              ggAACGCAACGTTTAATACGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGCCGTCTT‑CACCAGCGCTTTGACCCTCGCATACGCCTTACa                                                                  >  1:791710/1‑149 (MQ=255)
                                                                                   gCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTgg                                                             <  2:1334824/149‑1 (MQ=255)
                                                                                     aaCGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCa                                                           >  1:978232/1‑149 (MQ=255)
                                                                                     aaCGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCa                                                           >  2:1205981/1‑149 (MQ=255)
                                                                                      aCGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCAt                                                          >  2:1086253/1‑149 (MQ=255)
                                                                                       cGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATc                                                         <  2:264632/149‑1 (MQ=255)
                                                                                       cGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATc                                                         <  2:1438804/149‑1 (MQ=255)
                                                                                             aTCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAAt                                                   <  2:592471/149‑1 (MQ=255)
                                                                                              tCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATg                                                  >  1:164107/1‑149 (MQ=255)
                                                                                               ccGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATgg                                                 <  2:350301/149‑1 (MQ=255)
                                                                                                cGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGc                                                <  1:230111/149‑1 (MQ=255)
                                                                                                 gTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCa                                               >  1:1240962/1‑149 (MQ=255)
                                                                                                 gTAGTGTAGGTAACTCGTTAGCCAGCAAAATGGTGCGGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCa                                               <  1:1436771/149‑1 (MQ=255)
                                                                                                       tCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTACCCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCAc                                         >  2:415495/1‑149 (MQ=255)
                                                                                                       tCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCAc                                         >  1:1492112/1‑149 (MQ=255)
                                                                                                         ggTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTc                                       >  1:1509117/1‑149 (MQ=255)
                                                                                                          gTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTcg                                      >  2:1511619/1‑149 (MQ=255)
                                                                                                               tCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTGACAGCTGGCATCGAAAATGGCACGTCACTcgccgcc                                 <  1:1063773/149‑2 (MQ=255)
                                                                                                                  ttAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGc                              <  2:835158/149‑1 (MQ=255)
                                                                                                                     gCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCtt                           >  2:535648/1‑149 (MQ=255)
                                                                                                                      ccAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGCCGTCTT‑CCCCAGCGATTTACCCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTa                          >  1:63820/1‑149 (MQ=255)
                                                                                                                      ccAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTa                          >  1:818632/1‑149 (MQ=255)
                                                                                                                       cAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTAc                         >  2:193164/1‑149 (MQ=255)
                                                                                                                             aaTGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCgg                   <  1:1162130/149‑1 (MQ=255)
                                                                                                                                 ttGCTGAGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCaaa               >  1:1145768/2‑149 (MQ=255)
                                                                                                                                 gttcTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCaaa               <  2:1456470/146‑1 (MQ=255)
                                                                                                                                    cTGAGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagca            >  1:921569/1‑149 (MQ=255)
                                                                                                                                    cTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagca            >  2:1308680/1‑149 (MQ=255)
                                                                                                                                     tGAGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaa           >  1:641789/1‑149 (MQ=255)
                                                                                                                                     tGAGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaa           >  2:948306/1‑149 (MQ=255)
                                                                                                                                     tGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaa           >  2:1408675/1‑149 (MQ=255)
                                                                                                                                     tGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaa           <  1:535648/149‑1 (MQ=255)
                                                                                                                                       aGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaagc         >  2:1531263/1‑149 (MQ=255)
                                                                                                                                          ggAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCt      >  2:96533/1‑149 (MQ=255)
                                                                                                                                              aaaGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTgcgc  <  1:590691/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGC  >  NC_000913/1457836‑1458126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑