Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,568,919 T→C 7.9% intergenic (‑96/+35) yddW ← / ← gadC liprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,568,9190TC 7.9% 340.8 / 13.7 116intergenic (‑96/+35)yddW/gadCliprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base T (54/52);  new base C (7/2);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCG  >  NC_000913/1568772‑1569065
                                                                                                                                                   |                                                                                                                                                  
gTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTa                                                                                                                                                   <  2:428712/149‑1 (MQ=255)
 tATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTAt                                                                                                                                                  <  2:231270/149‑1 (MQ=255)
  aTCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATc                                                                                                                                                 <  2:1390075/149‑1 (MQ=255)
   tCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCg                                                                                                                                                >  2:465024/1‑149 (MQ=255)
      cTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGATCAGATATCCATATGTGCTGTTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCACCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTc                                                                                                                                             <  1:78306/149‑1 (MQ=255)
       tGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTcc                                                                                                                                            <  2:530545/149‑1 (MQ=255)
       tGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTcc                                                                                                                                            <  1:55293/149‑1 (MQ=255)
          tcttctAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCtt                                                                                                                                         >  1:1426041/1‑149 (MQ=255)
              ctAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTCCCtttttc                                                                                                                                     <  2:1363781/149‑5 (MQ=255)
              ctAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACATATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCt                                                                                                                                     >  1:16996/1‑149 (MQ=255)
                aaTCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATAATTCATCGCTCCCtttttcgt                                                                                                                                   <  1:465024/149‑7 (MQ=255)
                 aTCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTtat                                                                                                                                  <  1:1304790/149‑1 (MQ=255)
                    gTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAAc                                                                                                                               >  2:219198/1‑149 (MQ=255)
                    gTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAAc                                                                                                                               <  2:564445/149‑1 (MQ=255)
                     ttAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAAcc                                                                                                                              >  1:766856/1‑149 (MQ=255)
                       aaTTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACATCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCATTGTCTTATAACCAt                                                                                                                            >  1:227416/1‑149 (MQ=255)
                       aaTTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCAt                                                                                                                            >  2:1337726/1‑149 (MQ=255)
                        aTTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCAtt                                                                                                                           >  1:1326299/1‑149 (MQ=255)
                        aTTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCAtt                                                                                                                           <  1:1190969/149‑1 (MQ=255)
                        aTTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCAtt                                                                                                                           <  1:528665/149‑1 (MQ=255)
                             ttGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGAc                                                                                                                      >  1:1083438/1‑149 (MQ=255)
                               gTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACAt                                                                                                                    >  2:1358923/1‑149 (MQ=255)
                                tttCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATg                                                                                                                   >  2:2556/1‑149 (MQ=255)
                                tttCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATg                                                                                                                   <  1:1088916/149‑1 (MQ=255)
                                      gAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgt                                                                                                             >  1:934488/1‑149 (MQ=255)
                                        gCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAgtgt                                                                                                           >  2:1101435/1‑149 (MQ=255)
                                          aGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGttt                                                                                                         >  2:827791/1‑149 (MQ=255)
                                             ctgacATATGTGCTGGTTTCCGGTAAACAGATGTGCGCGCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCtt                                                                                                      <  1:688413/145‑1 (MQ=255)
                                                      gtgCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGtcattcat                                                                                             <  1:616638/149‑1 (MQ=255)
                                                      gtgCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATACCCGTTAAGTCAATACGACAGCAAGAACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGATTAGTGTTTCTTGtcattcat                                                                                             >  1:67172/1‑149 (MQ=255)
                                                       tgCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATtcatc                                                                                            <  1:753173/149‑1 (MQ=255)
                                                       tgCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATtcatc                                                                                            >  2:1195063/1‑149 (MQ=255)
                                                        gCTGGTTTCCTGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCTAGCACGAAAAAGGGTGCGATGTATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATtcatca                                                                                           <  1:12003/149‑1 (MQ=255)
                                                        gCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATtcatca                                                                                           <  1:349482/149‑1 (MQ=255)
                                                         cTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac                                                                                          >  2:1435972/1‑149 (MQ=255)
                                                          tGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcaca                                                                                         >  2:1595349/1‑149 (MQ=255)
                                                           ggTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa                                                                                        <  2:548586/149‑1 (MQ=255)
                                                           ggTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa                                                                                        <  2:440666/149‑1 (MQ=255)
                                                            gTTTCCTGTAAACAGATTTTCTCTCATTCTGGTACTTATCCACGTTAAGTCAATACGACTGCAAGGACTAAAAATGGAGCGATTAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAat                                                                                       <  1:939113/149‑1 (MQ=255)
                                                             tttCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAata                                                                                      >  2:1144377/1‑149 (MQ=255)
                                                               tcatgTAAACAGATGTGCGATCATTCTCGTACTTATCCCCGTTAAGTCAATACTACAGCAATCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTAAAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAtata                                                                                    <  1:206018/145‑1 (MQ=255)
                                                               tCCGGTAAACTGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATAAGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAtata                                                                                    <  1:733098/149‑1 (MQ=255)
                                                               tCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAtata                                                                                    <  2:316407/149‑1 (MQ=255)
                                                                 cGGTAAACAGATGTGCGCTCATTGTCGTACTTATCCCCGTTAAGTCAATACGACAGCATGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACtatatt                                                                                  <  2:802059/149‑3 (MQ=255)
                                                                   gTAAACAGATGTGCGCTCATTCTCGTTCTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtgt                                                                                <  2:1217619/149‑1 (MQ=255)
                                                                    tAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGtgtg                                                                               <  2:1539603/149‑1 (MQ=255)
                                                                      aaCAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt                                                                             <  1:1146210/149‑1 (MQ=255)
                                                                      aaCAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt                                                                             >  1:66306/1‑149 (MQ=255)
                                                                      aaCAGATGTGCGCTCATTCTAGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt                                                                             <  2:766856/149‑1 (MQ=255)
                                                                      aaCAGATGTGCGCTCATTATCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt                                                                             >  1:468867/1‑149 (MQ=255)
                                                                        cAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGa                                                                           <  2:1399051/149‑1 (MQ=255)
                                                                         aGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACTATATTGTGATGaa                                                                          <  1:209820/149‑1 (MQ=255)
                                                                            tgtgCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt                                                                       <  1:664681/149‑1 (MQ=255)
                                                                                 gcTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGGGCAcg                                                                  >  2:1003977/1‑149 (MQ=255)
                                                                                     aTTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCggg                                                              <  1:304636/149‑1 (MQ=255)
                                                                                     aTTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCggg                                                              >  1:513968/1‑149 (MQ=255)
                                                                                         tcGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCa                                                          >  2:738790/1‑149 (MQ=255)
                                                                                         tcGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCa                                                          >  2:920383/1‑149 (MQ=255)
                                                                                             aCTTATCCCCGTTAATTCAATAATACAGCAAGCACGTAAAATGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCAATCATCACAATATATTGTGGTGAACGTGCACGCGGGTGCAGgaa                                                      <  1:822291/149‑1 (MQ=255)
                                                                                               ttATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaaga                                                    >  2:1054288/1‑149 (MQ=255)
                                                                                               ttATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaaga                                                    >  2:768726/1‑149 (MQ=255)
                                                                                                tATCCCCGTTAAGTCAATACGACAGCAAGCACGAAATAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATTTAGTGTGGTGAACGTGCACTCGGTTGCATgaagaa                                                   <  2:1405221/149‑1 (MQ=255)
                                                                                                 aTCCCCGTTAAGTCAATACGACATCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag                                                  <  1:30771/149‑1 (MQ=255)
                                                                                                 aTCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag                                                  <  1:25565/149‑1 (MQ=255)
                                                                                                  tCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGt                                                 <  1:1476035/149‑1 (MQ=255)
                                                                                                     ccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAc                                              >  2:692399/1‑149 (MQ=255)
                                                                                                     ccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAc                                              >  2:665908/1‑149 (MQ=255)
                                                                                                     ccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAc                                              >  1:1046006/1‑149 (MQ=255)
                                                                                                     ccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAc                                              >  1:573147/1‑149 (MQ=255)
                                                                                                     ccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAc                                              >  2:1266934/1‑149 (MQ=255)
                                                                                                        ttAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtt                                           <  1:140024/149‑1 (MQ=255)
                                                                                                           aGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg                                        <  1:538429/149‑1 (MQ=255)
                                                                                                           aGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg                                        <  2:1326299/149‑1 (MQ=255)
                                                                                                             tCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGCCATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGt                                      >  2:58583/1‑149 (MQ=255)
                                                                                                                      cAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGa                             <  2:863923/149‑1 (MQ=255)
                                                                                                                       agcaagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACAATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGAc                            >  2:1400328/1‑149 (MQ=255)
                                                                                                                         caagcaCGAAAAAGGGAGCGTTGAATTATCGCTCCCTTGTCTTATAACCAATCAGAAATGGATAGTGTTTCTTGTAATTCAACACAATATAGTGTGGTGAAAGTGCACGCGGGTGAAGGAAGAAGTGACATTTTGGTTCGTTCTGAGTg                          >  1:1039246/1‑149 (MQ=255)
                                                                                                                         caagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACtt                          >  2:839232/1‑148 (MQ=255)
                                                                                                                         caagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTg                          >  2:837697/1‑149 (MQ=255)
                                                                                                                            gcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTg                       <  2:16996/149‑1 (MQ=255)
                                                                                                                            gcaCGAAAAAGGGAGCGATGAATTAACGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAAATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGAGCGTTCTGACTGTTg                       >  2:1541714/1‑149 (MQ=255)
                                                                                                                              aCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGTGTTCTGACTGTTGAt                     <  2:453181/149‑1 (MQ=255)
                                                                                                                              aCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGAt                     <  1:528555/149‑1 (MQ=255)
                                                                                                                                gAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTg                   >  2:568268/1‑149 (MQ=255)
                                                                                                                                gAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTg                   <  1:1358923/149‑1 (MQ=255)
                                                                                                                                   aaaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCt                >  1:952349/1‑149 (MQ=255)
                                                                                                                                    aaGGGAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTc               >  1:582061/1‑149 (MQ=255)
                                                                                                                                     aGGGAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTcc              >  2:1042487/1‑149 (MQ=255)
                                                                                                                                      gggAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCa             >  1:383464/1‑149 (MQ=255)
                                                                                                                                       ggAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCag            >  1:1103488/1‑149 (MQ=255)
                                                                                                                                        gAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCaga           <  2:468867/149‑1 (MQ=255)
                                                                                                                                        gAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGATGATTGGCTCCaga           <  1:219198/149‑1 (MQ=255)
                                                                                                                                        gAGCGATAATTCATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGATCCaga           >  2:987739/1‑149 (MQ=255)
                                                                                                                                          ccGATGAATAATCGCTCCCTTGTCATATATCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGt         <  2:362526/148‑1 (MQ=255)
                                                                                                                                            gATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGATGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa       >  2:1497938/1‑149 (MQ=255)
                                                                                                                                            gATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa       >  2:586827/1‑149 (MQ=255)
                                                                                                                                             aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAc      >  2:1593492/1‑149 (MQ=255)
                                                                                                                                                 aTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCg  >  1:340045/1‑149 (MQ=255)
                                                                                                                                                 aTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCg  <  2:513968/149‑1 (MQ=255)
                                                                                                                                                 aTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCg  >  2:1576909/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTATCGCTGGTCTTCTAATCGTTAATTTCTTGTTTCGGGAGCAGATATCCATATGTGCTGGTTTCCGGTAAACAGATGTGCGCTCATTCTCGTACTTATCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCG  >  NC_000913/1568772‑1569065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: