Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,647,873 C→T 6.0% intergenic (‑23/‑61) dicC ← / → dicA Qin prophage; DNA‑binding transcriptional regulator for DicB/Qin prophage; putative regulator for DicB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,647,8730CT 6.0% 476.0 / 11.3 149intergenic (‑23/‑61)dicC/dicAQin prophage; DNA‑binding transcriptional regulator for DicB/Qin prophage; putative regulator for DicB
Reads supporting (aligned to +/- strand):  ref base C (68/72);  new base T (5/4);  total (73/76)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 150 total aligned reads displayed.

ACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGC  >  NC_000913/1647725‑1648019
                                                                                                                                                    |                                                                                                                                                  
aCGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCCGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTc                                                                                                                                                    >  2:1035450/1‑149 (MQ=255)
 cGCATCGCGCGAACTTCTGGAACTATATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCa                                                                                                                                                   <  1:962328/149‑1 (MQ=255)
    aTCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaa                                                                                                                                                <  2:555107/149‑1 (MQ=255)
        cgcgACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaactaa                                                                                                                                            <  1:1429414/149‑1 (MQ=255)
        cgcgACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaactaa                                                                                                                                            >  1:1394536/1‑149 (MQ=255)
        cgcgACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCAtaactaa                                                                                                                                            <  2:1130019/149‑1 (MQ=255)
         gcgACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTTAtgactaac                                                                                                                                           <  2:812766/149‑1 (MQ=255)
           gACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTTATGACTAACaa                                                                                                                                         <  2:1466286/149‑1 (MQ=255)
            aCCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAACCCAAACGAATACCTTCTGCTTGTTCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAag                                                                                                                                        <  1:330520/149‑1 (MQ=255)
              cTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAagat                                                                                                                                      >  2:916106/1‑149 (MQ=255)
                   ggAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATg                                                                                                                                 >  1:1351307/1‑149 (MQ=255)
                   ggAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATg                                                                                                                                 >  2:1223518/1‑149 (MQ=255)
                   ggAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATg                                                                                                                                 >  1:1043368/1‑149 (MQ=255)
                    taaCTAAATCCCCTTTCAAGCTATAAAGCGAAGCCAAACGAATAGCTGCTGCTTGTGCAAGTTTTGTTTTTGATCCTAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTTATGACTAACAATAAAGgtgt                                                                                                                                <  2:1400006/148‑1 (MQ=255)
                     aaCTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGtt                                                                                                                               <  2:1201687/149‑1 (MQ=255)
                       cTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCCGTTATAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTaa                                                                                                                             >  2:267196/1‑149 (MQ=255)
                        tAAATCCCCTTTCCAGCTATAAAGCGAATCCAAACGAATACCTGATGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAAc                                                                                                                            <  1:1046058/149‑1 (MQ=255)
                        tAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAAc                                                                                                                            <  2:1432680/149‑1 (MQ=255)
                          aaTCCCCTTTCCAGCTATAAAGCGCAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGCAATACAAAAGAGCGTCAGTTTTACGCATTTAAAACCCCTTTATTGTTAGTCATAACTAACAAGATAGATGTAAACaa                                                                                                                          >  1:82662/1‑149 (MQ=255)
                          aaTCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACaa                                                                                                                                         >  2:1137602/1‑134 (MQ=255)
                          aaTCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACaa                                                                                                                          >  2:1230580/1‑149 (MQ=255)
                          aaTCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGAAAGTTTTGAATATGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACaa                                                                                                                          >  1:1543779/1‑149 (MQ=255)
                            tCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACaaaa                                                                                                                        >  1:1565271/1‑149 (MQ=255)
                             ccccTTTCCAGCTATAAAGCGAAGCCAAACTAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACaaaaa                                                                                                                       <  2:1572149/149‑1 (MQ=255)
                             ccccTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACaaaaa                                                                                                                       <  1:35765/149‑1 (MQ=255)
                              cccTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAAc                                                                                                                      >  2:776290/1‑149 (MQ=255)
                              cccTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAAc                                                                                                                      <  1:627155/149‑1 (MQ=255)
                               ccTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACa                                                                                                                     >  2:549448/1‑149 (MQ=255)
                                 tttCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATa                                                                                                                   <  2:92063/149‑1 (MQ=255)
                                        cTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATa                                                                                                            <  2:1375033/149‑1 (MQ=255)
                                        cTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATa                                                                                                            <  1:502532/149‑1 (MQ=255)
                                                cGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAg                                                                                                    <  2:1506355/149‑1 (MQ=255)
                                                 gAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAAAACCTTTATTGTTAGTCATAACTAAAAAGATAGATGTTAACAAAAACATAGTCAATACTATATAga                                                                                                   >  2:1509698/1‑148 (MQ=255)
                                                   aGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCAt                                                                                                 <  1:1518665/149‑1 (MQ=255)
                                                   aGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCAt                                                                                                 <  2:756862/149‑1 (MQ=255)
                                                       aaaCGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAATAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGc                                                                                             <  2:1444497/149‑1 (MQ=255)
                                                       aaaCGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGc                                                                                             >  1:601234/1‑149 (MQ=255)
                                                        aaCGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCt                                                                                            <  2:236897/149‑1 (MQ=255)
                                                                ccTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATgg                                                                                    <  2:567361/149‑1 (MQ=255)
                                                                actgctgCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTAAGTTTTAAGAATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATgg                                                                                    <  1:969417/148‑1 (MQ=255)
                                                                  tgctgcTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGAATAAGCTAACTATGCaa                                                                                  >  2:324758/1‑149 (MQ=255)
                                                                   gctgctTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGaaa                                                                                 <  2:828735/149‑1 (MQ=255)
                                                                       ctTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTTAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCAAACTATGGAAACaaa                                                                             >  1:1281450/1‑149 (MQ=255)
                                                                       ctTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACTATTTAGCATTAGCTAACTATGGAAACaaa                                                                             >  1:1275199/1‑149 (MQ=255)
                                                                       ctTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACaaa                                                                             <  1:1035450/149‑1 (MQ=255)
                                                                           tgCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACTATTTAGCATTAGCTAACTATGGAAACAAAAAAt                                                                         >  2:174241/1‑149 (MQ=255)
                                                                           tgCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAAt                                                                         >  2:608521/1‑149 (MQ=255)
                                                                             cAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAAttt                                                                       >  1:994407/1‑149 (MQ=255)
                                                                             cAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAAttt                                                                       >  2:1517546/1‑149 (MQ=255)
                                                                              aaGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTa                                                                      >  1:1376538/1‑149 (MQ=255)
                                                                               aGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTaa                                                                     <  1:1134589/149‑1 (MQ=255)
                                                                                  tttgttttTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTa                                                                  >  2:1182202/1‑149 (MQ=255)
                                                                                   ttgttttTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTAt                                                                 >  2:517398/1‑149 (MQ=255)
                                                                                    tgttttTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATc                                                                >  2:363545/1‑149 (MQ=255)
                                                                                      tttttGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCgg                                                              <  2:926595/149‑1 (MQ=255)
                                                                                      tttttGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCgg                                                              >  1:434815/1‑149 (MQ=255)
                                                                                        tttGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCg                                                            >  2:1015141/1‑149 (MQ=255)
                                                                                          tGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGaa                                                          <  2:1394536/149‑1 (MQ=255)
                                                                                               cGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCAt                                                     <  1:916106/149‑1 (MQ=255)
                                                                                                gAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATc                                                    <  1:1230580/149‑1 (MQ=255)
                                                                                                 aaaTACAAAATAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCa                                                   <  1:832183/149‑1 (MQ=255)
                                                                                                 aaaTACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCa                                                   >  2:412977/1‑149 (MQ=255)
                                                                                                 aaaTACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCa                                                   >  1:1019233/1‑149 (MQ=255)
                                                                                                  aaTACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAg                                                  <  1:424217/149‑1 (MQ=255)
                                                                                                  aaTACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAg                                                  <  1:549448/149‑1 (MQ=255)
                                                                                                                tCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTATCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaa                                    <  2:184951/149‑1 (MQ=255)
                                                                                                                 cAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaaa                                   <  2:466408/149‑1 (MQ=255)
                                                                                                                 cAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaaa                                   >  2:1345490/1‑149 (MQ=255)
                                                                                                                  aGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAAc                                  <  2:223785/149‑1 (MQ=255)
                                                                                                                  aGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAAc                                  <  1:1149887/149‑1 (MQ=255)
                                                                                                                        aaGCATTTAAAACACCTTTATTGTTAGTCATAAATAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCaaa                            <  2:278029/149‑1 (MQ=255)
                                                                                                                            aTTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTATCTAACTATGGAAACAAAACATTTAACTATCGGCTAACGCATCAGGTATCGTCGGAAAAACCTGAAacaca                        <  2:1543779/149‑1 (MQ=255)
                                                                                                                             tttAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAAACTCAAacacac                       >  2:505187/1‑149 (MQ=255)
                                                                                                                                 aaaCACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCaa                   >  2:114047/1‑149 (MQ=255)
                                                                                                                                  aacaacTTTATTGTTAGTTATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATTCGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCaaa                  <  1:213055/149‑1 (MQ=255)
                                                                                                                                  aaCACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATc                                                    >  1:249179/1‑115 (MQ=255)
                                                                                                                                  aaCACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATc                                                    <  2:249179/115‑1 (MQ=255)
                                                                                                                                   acacCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAg                 <  2:601234/149‑1 (MQ=255)
                                                                                                                                   acacCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAg                 <  1:772386/149‑1 (MQ=255)
                                                                                                                                    cacCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAgt                >  2:831964/1‑148 (MQ=255)
                                                                                                                                     atctatcttGTTAGTTATGACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGt               >  2:993220/8‑149 (MQ=255)
                                                                                                                                       cTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGtct             >  2:1596205/1‑149 (MQ=255)
                                                                                                                                       cTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGtct             >  1:891849/1‑149 (MQ=255)
                                                                                                                                       cTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGact             >  2:1573515/1‑149 (MQ=255)
                                                                                                                                        tttATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGtctc            >  1:440752/1‑149 (MQ=255)
                                                                                                                                        tttATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGtctc            >  1:1563524/1‑149 (MQ=255)
                                                                                                                                        tttATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGtctc            >  2:691005/1‑149 (MQ=255)
                                                                                                                                        tttATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGtctc            >  2:819604/1‑149 (MQ=255)
                                                                                                                                          tcttGTTAGTTATGACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCtt          >  1:1089389/3‑149 (MQ=255)
                                                                                                                                           aTTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTg         <  1:151245/149‑1 (MQ=255)
                                                                                                                                           aTTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTg         >  1:1128251/1‑149 (MQ=255)
                                                                                                                                            ttGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGGCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGc        <  2:1043368/149‑1 (MQ=255)
                                                                                                                                               ttAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTaa     >  1:342476/1‑149 (MQ=255)
                                                                                                                                               ttAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTaa     >  1:711421/1‑149 (MQ=255)
                                                                                                                                                 aGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAg   <  2:1475629/149‑1 (MQ=255)
                                                                                                                                                  gTCATAACTAACAAGATAGATTTTAACAAAAACATAGTAAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGc  <  1:1509698/149‑1 (MQ=255)
                                                                                                                                                  gTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCCAAGGTCTCTTGCTCAAGc  >  2:314989/1‑149 (MQ=255)
                                                                                                                                                  gTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGc  >  2:701702/1‑149 (MQ=255)
                                                                                                                                                   tCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACg                                                        >  1:110595/1‑94 (MQ=255)
                                                                                                                                                   tCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACg                                                        <  2:110595/94‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACGCATCGCGCGACCTTCGGGAACTAAATCCCCTTTCCAGCTATAAAGCGAAGCCAAACGAATACCTGCTGCTTGTGCAAGTTTTGTTTTTGAACCGAAATACAAAAGAGCGTCAGTTTTAAGCATTTAAAACACCTTTATTGTTAGTCATAACTAACAAGATAGATGTTAACAAAAACATAGTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGC  >  NC_000913/1647725‑1648019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: