Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,744,294 T→A 5.1% F280I (TTT→ATT)  mdtK → multidrug efflux system transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,2940TA 5.1% 286.1 / 3.0 90F280I (TTT→ATT) mdtKmultidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base T (37/48);  new base A (3/2);  total (40/50)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGAC  >  NC_000913/1744146‑1744442
                                                                                                                                                    |                                                                                                                                                    
cGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACt                                                                                                                                                      >  1:490636/1‑149 (MQ=255)
 gATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACtt                                                                                                                                                     >  2:145192/1‑149 (MQ=255)
 gATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACtt                                                                                                                                                     <  1:1329074/149‑1 (MQ=255)
   tCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTa                                                                                                                                                   <  2:702329/149‑1 (MQ=255)
   tCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTa                                                                                                                                                   <  1:384886/149‑1 (MQ=255)
    ccTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGGTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAg                                                                                                                                                  <  1:231267/149‑1 (MQ=255)
     cTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACTAGATTGCCCTGAACTTTAGt                                                                                                                                                 <  2:426357/149‑1 (MQ=255)
     cTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGt                                                                                                                                                 <  2:866201/149‑1 (MQ=255)
      tGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACATTAGtg                                                                                                                                                >  2:1313997/1‑148 (MQ=255)
         ggTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCAc                                                                                                                                             <  2:518931/149‑1 (MQ=255)
         ggTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCAc                                                                                                                                             <  2:1297241/149‑1 (MQ=255)
           ttATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTa                                                                                                                                           >  2:777685/1‑149 (MQ=255)
           ttATGAAACGACTGATTCAACTCGGTTTGCCGAATGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTa                                                                                                                                           <  1:770305/149‑1 (MQ=255)
             aTGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAAt                                                                                                                                         <  2:591237/149‑1 (MQ=255)
                aaaCGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGtt                                                                                                                                      >  2:1494694/1‑149 (MQ=255)
                 aaCGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTc                                                                                                                                     <  2:1345650/149‑1 (MQ=255)
                 aaCGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTc                                                                                                                                     <  1:626205/149‑1 (MQ=255)
                  aCGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCg                                                                                                                                    <  1:435373/149‑1 (MQ=255)
                   cGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACATTAGTGAACTAAAGTTcag                                                                                                                                   <  1:1313997/149‑3 (MQ=255)
                   cGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACATTAGTGAACTAAAGTTcag                                                                                                                                   <  1:198567/149‑3 (MQ=255)
                    gACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGATCGGTATTGTTGATGTCTCATGACACCAGATTGACCTTAACTTTAGTTCAATAATGTTAGTg                                                                                                                                  >  2:169128/1‑149 (MQ=255)
                     aCTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGc                                                                                                                                 <  1:1501515/149‑1 (MQ=255)
                     aCTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGc                                                                                                                                 <  2:347810/149‑1 (MQ=255)
                      cTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCt                                                                                                                                <  1:604273/149‑1 (MQ=255)
                       tGATTTAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGGCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCtt                                                                                                                               <  2:884361/149‑1 (MQ=255)
                         aTTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTcc                                                                                                                             >  1:389681/1‑149 (MQ=255)
                          ttCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCa                                                                                                                            >  1:1594130/1‑149 (MQ=255)
                            aaaCTCGGTTTGCCTATTGCGCTGTAACTGTTCTTTGAAGTGACACTGTTGGCCGTCGTGGCTCTGGTATTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAAt                                                                                                                          <  2:907375/148‑1 (MQ=255)
                             aaCTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATg                                                                                                                         >  1:1241038/1‑149 (MQ=255)
                             aaCTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATg                                                                                                                         <  2:440255/149‑1 (MQ=255)
                               cTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTc                                                                                                                       >  2:359341/1‑149 (MQ=255)
                                    tttGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCTCTgg                                                                                                                  <  1:145192/149‑1 (MQ=255)
                                          gATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAg                                                                                                            <  2:1265654/149‑1 (MQ=255)
                                           aTTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCTGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGc                                                                                                           >  1:921356/1‑149 (MQ=255)
                                           aTTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGc                                                                                                           >  2:232418/1‑149 (MQ=255)
                                             tGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCgg                                                                                                         <  2:389681/149‑1 (MQ=255)
                                                gcTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTaa                                                                                                      <  1:1268109/149‑1 (MQ=255)
                                                  tGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACt                                                                                                    >  2:1400727/1‑149 (MQ=255)
                                                  gggCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTTTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCTCTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACt                                                                                                    <  2:275929/148‑1 (MQ=255)
                                                    gCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTAt                                                                                                  <  1:846806/149‑1 (MQ=255)
                                                      aCTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATcc                                                                                                >  1:862393/1‑149 (MQ=255)
                                                      aCTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATcc                                                                                                >  1:427504/1‑149 (MQ=255)
                                                        tGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCcgc                                                                                              >  2:199064/1‑149 (MQ=255)
                                                          ttCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGt                                                                                            >  1:1316894/1‑149 (MQ=255)
                                                             tttGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAgg                                                                                         <  1:1576401/149‑1 (MQ=255)
                                                             tttGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAgg                                                                                         >  2:840945/1‑149 (MQ=255)
                                                                gAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCAACCAATGACGCTGGCGGCAGCGGTAACTATCCGCGTACGTTa                                                                                      >  2:1555415/1‑149 (MQ=255)
                                                                  aGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATc                                                                                    >  2:1509623/1‑149 (MQ=255)
                                                                    tGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGt                                                                                  >  2:403151/1‑149 (MQ=255)
                                                                    agccacTGATTGCCGTCCTGGCTCTGTTAGTGTCACCGCTCGGTATAGTTGATGTAGCAGGACACCAGATTGCCCAGAACATTATTTCACTAATGTTCGTGCTTCCAATCTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTaaagt                                                                                  >  2:862269/4‑145 (MQ=255)
                                                                     gACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTc                                                                                 >  1:187058/1‑149 (MQ=255)
                                                                      acacTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCt                                                                                >  2:9797/1‑149 (MQ=255)
                                                                        acTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTGCCAATGTCGCTGGCGGCAGCGGTATCTATCCGCGTAGGTTTTCGGCTgg                                                                              >  2:784666/1‑149 (MQ=255)
                                                                         cTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTggg                                                                             <  2:938444/149‑1 (MQ=255)
                                                                                  gtcgtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTGCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTc                                                                    <  2:1203300/149‑1 (MQ=255)
                                                                                   tcgtGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCa                                                                   >  1:423376/1‑149 (MQ=255)
                                                                                       ggCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGc                                                               <  1:1400727/149‑1 (MQ=255)
                                                                                         ctctGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTg                                                             <  1:814749/149‑1 (MQ=255)
                                                                                          tctGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTgg                                                            >  1:379176/1‑149 (MQ=255)
                                                                                             gTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATg                                                         >  1:1506402/1‑149 (MQ=255)
                                                                                              ttAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATgc                                                        >  1:133181/1‑149 (MQ=255)
                                                                                              ttAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATgc                                                        >  1:1218317/1‑149 (MQ=255)
                                                                                              ttAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATgc                                                        >  2:526825/1‑149 (MQ=255)
                                                                                              ttAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATgc                                                        <  1:1491173/149‑1 (MQ=255)
                                                                                               tAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATgcg                                                       <  2:133181/149‑1 (MQ=255)
                                                                                               tAGTGTCTCCGCTCGGTATTGGTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTGACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATgcg                                                       <  2:416589/149‑1 (MQ=255)
                                                                                                    tcgccGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAAc                                                  <  1:1142590/146‑1 (MQ=255)
                                                                                                                 ttgttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCt                                                              <  2:195282/124‑1 (MQ=255)
                                                                                                                 ttgttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCt                                                              >  1:195282/1‑124 (MQ=255)
                                                                                                                 ttgttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGAcc                                     >  2:1107368/1‑149 (MQ=255)
                                                                                                                  tgttgATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCg                                    <  1:9797/149‑1 (MQ=255)
                                                                                                                     tcctGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGCTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGc                                 <  2:1480376/146‑1 (MQ=255)
                                                                                                                      gATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCt                                >  1:1350660/1‑149 (MQ=255)
                                                                                                                       aTGTCGCAGGACACCAGATTGCCCTGCACTTTAGTTCACTAATGTTCGAGCTTCCAATGTCGATGGCGGCATCGGTAACTATCCGCGTAAGTTATCGTCATGGTACGGGCTACCAGCTGCCTGCGCAAACCGCTTCGAGTACCGGGCtt                               >  2:983266/1‑149 (MQ=255)
                                                                                                                            gCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggt                          <  1:687965/149‑1 (MQ=255)
                                                                                                                            gCAGGACACCAGATTGCCCTGAACTATACTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGTCAGGGCATAtggt                          >  1:1188807/1‑149 (MQ=255)
                                                                                                                             cAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggtg                         <  1:172924/149‑1 (MQ=255)
                                                                                                                             cAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggtg                         <  1:23462/149‑1 (MQ=255)
                                                                                                                             cAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTAAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggtg                         <  2:1594130/149‑1 (MQ=255)
                                                                                                                              cggACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGGCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggtgg                        <  2:196814/148‑1 (MQ=255)
                                                                                                                              agtacacCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggtgg                        <  2:456753/146‑1 (MQ=255)
                                                                                                                              aGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTAtggtgg                        >  2:427024/1‑149 (MQ=255)
                                                                                                                               ggACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATggtggg                       <  1:1555415/149‑1 (MQ=255)
                                                                                                                               ggACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCGGGATGCGCAAACCGCTGCGCGGACCGGGCTTATggtggg                       >  2:286468/1‑149 (MQ=255)
                                                                                                                                gACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGCCCGGGCTTATggtgggt                      >  2:1341114/1‑149 (MQ=255)
                                                                                                                                  cacCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGgtgt                    <  2:490636/149‑1 (MQ=255)
                                                                                                                                  cacCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGgtgt                    <  1:1494694/149‑1 (MQ=255)
                                                                                                                                       gATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCTCAAACCGCTGCTCGGACCGGGCTTATGGTGGGTGTCTGTa               <  2:473649/149‑1 (MQ=255)
                                                                                                                                          tGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATgg            <  1:840945/149‑1 (MQ=255)
                                                                                                                                            cccTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTATCTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAACCCGCTGCGCGGACCGGGCTTATGGTGGGTGTCGGTATTGCa          >  1:659224/1‑149 (MQ=255)
                                                                                                                                              acgAACATTAGTGAACTAAAGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAAc        >  1:437134/3‑149 (MQ=255)
                                                                                                                                               tGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCTCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAAcc       >  2:288433/1‑149 (MQ=255)
                                                                                                                                                 aaCTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGGCTGTATGGCAACCCt     <  2:427504/149‑1 (MQ=255)
                                                                                                                                                    tttAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGTAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGAc  >  1:943614/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGGTATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTAATGTTCGTGCTTCCAATGTCGCTGGCGGCAGCGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATGGTGGGTGTCTGTATGGCAACCCTGAC  >  NC_000913/1744146‑1744442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: