Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,747,153	G→T	7.2%	G8V (GGT→GTT)	ydhS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,747,153	0	G	T	7.2%	226.7 / 2.2	76	G8V (GGT→GTT)	ydhS	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (38/32);  new base T (2/4);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATT  >  NC_000913/1747005‑1747301                                                                                                                                                     |                                                                                                                                                     aCAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTtgg                                                                                                                                                      >  2:135251/1‑149 (MQ=255)         tAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGc                                                                                                                                              <  2:150000/149‑1 (MQ=255)          aaTCACGGGCGGTTAGCGCCCTTTTCATCCCTGGCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGcc                                                                                                                                             <  2:1115041/149‑1 (MQ=255)           aTCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCt                                                                                                                                            >  2:950617/1‑149 (MQ=255)            tCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTa                                                                                                                                           >  1:170209/1‑149 (MQ=255)            tCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTa                                                                                                                                           >  2:518028/1‑149 (MQ=255)            tCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTa                                                                                                                                           >  2:188673/1‑149 (MQ=255)             cAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGTTTCCGGGCCTAc                                                                                                                                          >  2:139464/1‑149 (MQ=255)              aaGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACg                                                                                                                                         <  1:1556279/149‑1 (MQ=255)               aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                        >  2:1293099/1‑149 (MQ=255)                 ggCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgggg                                                                                                                                      >  2:947650/1‑149 (MQ=255)                  gCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGa                                                                                                                                     <  1:44613/149‑1 (MQ=255)                   cGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt                                                                                                                                    <  2:1191270/149‑1 (MQ=255)                   cGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt                                                                                                                                    <  1:1099135/149‑1 (MQ=255)                    ggTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATc                                                                                                                                   <  1:1281700/149‑1 (MQ=255)                     gTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGGGCCGGGCCTACGGGGATCt                                                                                                                                  <  2:851844/149‑1 (MQ=255)                      ttAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTa                                                                                                                                 <  1:103659/149‑1 (MQ=255)                      ttAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAAACAAACTTATCTGATAACAATAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTa                                                                                                                                 <  2:35378/149‑1 (MQ=255)                         gcgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGAGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTacac                                                                                                                              >  2:780596/1‑149 (MQ=255)                           gcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCt                                                                                                                            >  2:1569459/1‑149 (MQ=255)                            cccTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCtt                                                                                                                           >  2:1235637/1‑149 (MQ=255)                            cccTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCtt                                                                                                                           >  2:1117824/1‑149 (MQ=255)                             ccTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTa                                                                                                                          <  1:1569459/149‑1 (MQ=255)                             ccTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTa                                                                                                                          <  1:197324/149‑1 (MQ=255)                                         tGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATTTTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTa                                                                                                              <  2:432251/149‑1 (MQ=255)                                         tGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTa                                                                                                              <  1:448274/149‑1 (MQ=255)                                            cTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAg                                                                                                           >  2:1077882/1‑149 (MQ=255)                                             tGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTATCGCTTCTACAGc                                                                                                          >  1:1107828/1‑149 (MQ=255)                                              gAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGca                                                                                                         >  2:1581757/1‑149 (MQ=255)                                                 aTTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACATTATTCTCGCTTCTACAGcaaca                                                                                                      >  1:172379/1‑149 (MQ=255)                                                   ttCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACaaa                                                                                                    <  1:659861/149‑1 (MQ=255)                                                      tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                                 >  2:1297685/1‑149 (MQ=255)                                                         aaTTCTAAAAATCTCAACCTAACTTATCTGATAACACTAAATTCGAAATAATGCGTACAGGTAAGTAACAATGAAAATAATTGCTATTGTGGGGGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCAc                                                                                              <  1:1533353/149‑1 (MQ=255)                                                          tttCTAAAAATCTCAACCAAACTTATCTGTTAACACTAAATTCGAAAGAATGGGTACAGGTAAGTAACAATGAAATAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAGCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACt                                                                                             <  1:1117458/148‑1 (MQ=255)                                                            tCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAATAATTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACttt                                                                                           <  2:1389927/149‑1 (MQ=255)                                                              tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttct                                                                                         <  1:1235637/149‑1 (MQ=255)                                                              tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGCGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttct                                                                                         >  1:479880/1‑149 (MQ=255)                                                               aaaaaTCTCAACCAAACTTATCTTATAACACAAAATTCGAAAGAATGCGTACAGGTAAGTAACATTGAAAATTATTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttcta                                                                                        <  2:355124/149‑1 (MQ=255)                                                               aaaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttcta                                                                                        >  1:1478171/1‑149 (MQ=255)                                                                aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctat                                                                                       >  1:1151518/1‑149 (MQ=255)                                                                aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctat                                                                                       >  1:1052362/1‑149 (MQ=255)                                                                  aaTCTCACCCATACTTATCTGATAACACTAAATTCGAAAGAATTCGTTCAGGTATGTAACTATGATAATTTTTTTTTTTTTTGTTTCGGTGCCTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctattt                                                                                     <  2:762858/149‑1 (MQ=255)                                                                       cAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATc                                                                                <  1:551591/149‑1 (MQ=255)                                                                        aaCCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCt                                                                               >  2:281942/1‑149 (MQ=255)                                                                             aaCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAg                                                                          <  1:950617/149‑1 (MQ=255)                                                                             aaCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAg                                                                          <  1:518028/149‑1 (MQ=255)                                                                                       aTAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTggg                                                                                                                                                      <  2:1513201/63‑1 (MQ=255)                                                                                       aTAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTggg                                                                                                                                                      >  1:1513201/1‑63 (MQ=255)                                                                                           cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                            <  1:717188/149‑1 (MQ=255)                                                                                            acTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGcc                                                           >  1:669194/1‑149 (MQ=255)                                                                                            acTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGcc                                                           >  1:804173/1‑149 (MQ=255)                                                                                             cTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGATATTGTGTTTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTAAAGCAACAAACTCAACTATCTAATTCTATCTTCGAGCAGGCTGACGACGCCg                                                          >  1:219083/1‑149 (MQ=255)                                                                                             atAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCg                                                          <  1:1117824/148‑1 (MQ=255)                                                                                                aaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgtg                                                       >  2:1442548/1‑149 (MQ=255)                                                                                                aaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgtg                                                       >  2:1366840/1‑149 (MQ=255)                                                                                                    cGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGGGTCgg                                                   <  1:1208671/149‑1 (MQ=255)                                                                                                     gAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCggg                                                  >  2:944210/1‑149 (MQ=255)                                                                                                     gAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCggg                                                  >  2:1152524/1‑149 (MQ=255)                                                                                                     gAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCggg                                                  >  2:1240091/1‑149 (MQ=255)                                                                                                      aaaGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGa                                                 >  2:1087142/1‑149 (MQ=255)                                                                                                      aaaGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTAGGTGCAGGTCCTACGGGTATCTACACCTTATTCTAGATTCTACAGCAACAAACTCCACTTTCTATTTCTATCATCGAGCAGGCTGACCAGGCAGGAATCggaa                                                 >  1:52485/1‑147 (MQ=255)                                                                                                       aaGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGAt                                                <  1:1343958/149‑1 (MQ=255)                                                                                                          aaTGCGTACAGGTAAGTAACAATGAAAAATATTGATATTTTGGGTTCCGGGACTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGcc                                             <  2:601660/149‑1 (MQ=255)                                                                                                          aaTGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGcc                                             >  2:1260863/1‑149 (MQ=255)                                                                                                             gcctACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCAATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATa                                          <  1:780596/146‑1 (MQ=255)                                                                                                                   aGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtga                                    >  2:612893/1‑149 (MQ=255)                                                                                                                   aGGTAAGTAACAATGAAAAAAATTACTATTGTGGGAGCCGGGCCTACGGGGATCTACACCTTAATCACCCTACTACAGCAACAAACTCCACTTTCTATTTCTATCTGCGAGCAGGCTCACGAGGCCGGTTTCGGGATGCCATACAGtga                                    >  2:126926/1‑149 (MQ=255)                                                                                                                    ggTAAGTAACAATGAAAAAAATTGCTGTTGTGGGTGCCGGGCCTACGGGGACCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgat                                   <  1:87396/149‑1 (MQ=255)                                                                                                                       aagtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAg                                >  2:659252/1‑149 (MQ=255)                                                                                                                          taaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaa                             >  2:750163/1‑149 (MQ=255)                                                                                                                          taaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaa                             >  2:883617/1‑149 (MQ=255)                                                                                                                            aCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaaaa                           <  1:716126/149‑1 (MQ=255)                                                                                                                             cAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTACTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCGGGTGTCGGGATGCCATAAAGTTATGAGGAAAAc                          >  1:359763/1‑149 (MQ=255)                                                                                                                                  aaaaaaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaa                     >  2:596497/1‑149 (MQ=255)                                                                                                                                       aaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatga                <  2:1478171/149‑1 (MQ=255)                                                                                                                                       aaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatga                <  1:1442548/149‑1 (MQ=255)                                                                                                                                             tATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTgg          <  1:1260863/149‑1 (MQ=255)                                                                                                                                                  tgGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAat     >  2:1041430/1‑149 (MQ=255)                                                                                                                                                  tgGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAat     <  1:1152524/149‑1 (MQ=255)                                                                                                                                                    ggTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATATAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAatat   >  2:870166/1‑149 (MQ=255)                                                                                                                                                    ggTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAatat   >  1:1556546/1‑149 (MQ=255)                                                                                                                                                     gTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATAtt  >  2:1206256/1‑149 (MQ=255)                                                                                                                                                     gTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATAtt  <  1:1041430/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     ACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATT  >  NC_000913/1747005‑1747301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑