Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,763,451 C→T 7.0% V187M (GTG→ATG) ‡ sufB ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,763,4510CT 7.0% 310.9 / 7.3 99V187M (GTG→ATG) ‡sufBcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base C (49/43);  new base T (2/5);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTG  >  NC_000913/1763304‑1763599
                                                                                                                                                   |                                                                                                                                                    
cAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTGCCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACg                                                                                                                                                     <  1:1238273/149‑1 (MQ=255)
   aCTGCCCGGGTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTg                                                                                                                                                  <  1:210653/149‑1 (MQ=255)
    cTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGc                                                                                                                                                 <  2:1547265/149‑1 (MQ=255)
     tGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGcc                                                                                                                                                <  2:422670/149‑1 (MQ=255)
      gCCCGGTTGTTTCTGCGGTAATGCGAAAATAGGTGGAAAGTTCGATCGGGCAGCGCACGCCTTTAGGCACATAATTAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCg                                                                                                                                               <  1:92454/149‑1 (MQ=255)
       cccGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCga                                                                                                                                              <  2:1597107/149‑1 (MQ=255)
         cGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCCCCCCGGTGCCgaga                                                                                                                                            >  2:1365127/1‑149 (MQ=255)
         cGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCGTGGTGCCgggg                                                                                                                                            <  2:613719/149‑4 (MQ=255)
         cGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCgaga                                                                                                                                            >  2:528308/1‑149 (MQ=255)
          ggTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCGTGGTGCCgggga                                                                                                                                           <  1:400421/149‑5 (MQ=255)
          ggTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGat                                                                                                                                           >  2:1309869/1‑149 (MQ=255)
          ggTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGat                                                                                                                                           >  2:48775/1‑149 (MQ=255)
           gTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCTCGGCACCGTGGTGCCggggaa                                                                                                                                          <  2:180582/149‑6 (MQ=255)
           gTTTTTTCTGAGTTAATGCGAAAATATGTGTAAAGTTCCATAGTTAATAGATATATTTTAGGCATATTATTTTTCGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCTCGGCACCGTGGTGCCggggaa                                                                                                                                          <  2:127417/149‑6 (MQ=255)
                ttCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTAc                                                                                                                                     >  1:1265809/1‑149 (MQ=255)
                   tGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCa                                                                                                                                  <  1:550157/149‑1 (MQ=255)
                   tGCGTTAATGCGAAAAAAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCa                                                                                                                                  >  2:886160/1‑149 (MQ=255)
                      gTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCa                                                                                                                               >  1:899603/1‑149 (MQ=255)
                      gTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCa                                                                                                                               >  1:1446574/1‑149 (MQ=255)
                       ttAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACAATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAg                                                                                                                              >  1:980435/1‑149 (MQ=255)
                          aTGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTc                                                                                                                           >  2:318406/1‑149 (MQ=255)
                           tGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTcc                                                                                                                          >  1:375758/1‑149 (MQ=255)
                             cGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCgg                                                                                                                        <  1:980217/149‑1 (MQ=255)
                             cGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCgg                                                                                                                        <  2:644901/149‑1 (MQ=255)
                              gAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCggg                                                                                                                       >  2:863876/1‑149 (MQ=255)
                                aaaTAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTg                                                                                                                     >  2:1431750/1‑149 (MQ=255)
                                   tAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATc                                                                                                                  <  2:1396664/149‑1 (MQ=255)
                                      gTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTg                                                                                                               <  1:220326/149‑1 (MQ=255)
                                      gTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGAGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTg                                                                                                               >  2:1561810/1‑149 (MQ=255)
                                       tGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTgg                                                                                                              >  2:877722/1‑149 (MQ=255)
                                       tGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTgg                                                                                                              <  2:1517517/149‑1 (MQ=255)
                                         gAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGAt                                                                                                            >  2:576553/1‑149 (MQ=255)
                                          aaaGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATc                                                                                                           <  2:211450/149‑1 (MQ=255)
                                            aGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGc                                                                                                         <  2:1093883/149‑1 (MQ=255)
                                             gTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGcc                                                                                                        <  1:1270367/149‑1 (MQ=255)
                                                    cGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCaa                                                                                                 <  2:694447/149‑1 (MQ=255)
                                                      ggCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAg                                                                                               <  2:462178/149‑1 (MQ=255)
                                                        cAGCGCACGCCTTTAGGCTCATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAggc                                                                                             >  2:631064/1‑148 (MQ=255)
                                                          gcgcACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc                                                                                           <  1:7885/149‑1 (MQ=255)
                                                          gcgcACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc                                                                                           <  1:366664/149‑1 (MQ=255)
                                                          gcgcACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGGGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAAc                                                                                           <  2:864725/149‑1 (MQ=255)
                                                            gcACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCCCCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAg                                                                                         >  1:1113669/1‑149 (MQ=255)
                                                             cACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGa                                                                                        >  2:527766/1‑149 (MQ=255)
                                                                  cTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaata                                                                                   >  2:808401/1‑149 (MQ=255)
                                                                  cTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaata                                                                                   >  2:907992/1‑149 (MQ=255)
                                                                  cTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAaata                                                                                   >  1:575086/1‑149 (MQ=255)
                                                                     tAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAAtt                                                                                <  2:592431/149‑1 (MQ=255)
                                                                     tAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAAtt                                                                                <  2:778571/149‑1 (MQ=255)
                                                                      aGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTc                                                                               >  1:224784/1‑149 (MQ=255)
                                                                       ggCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTACGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTCCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTcc                                                                              <  1:760095/149‑1 (MQ=255)
                                                                          acaTAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTg                                                                           <  2:1411121/149‑1 (MQ=255)
                                                                          acaTAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTg                                                                           >  1:145853/1‑149 (MQ=255)
                                                                           caTAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGc                                                                          >  2:899898/1‑149 (MQ=255)
                                                                            ataaataaACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCCCCAAAGGCACAGAAAATAATTCCCTGCt                                                                         >  2:807917/1‑149 (MQ=255)
                                                                            ataaataaACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCt                                                                         >  2:515822/1‑149 (MQ=255)
                                                                               aataaaCGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCCGCACCACGGTGCCCAGATATTTACGCACCAGTTCCGGGTGCTCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCg                                                                      >  2:1216693/1‑149 (MQ=255)
                                                                                ataaaCGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGc                                                                     >  2:586747/1‑149 (MQ=255)
                                                                                ataaaCGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGc                                                                     <  2:169368/149‑1 (MQ=255)
                                                                                      gTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCCCCAGTTCCGGGTGATCGTGGATCGCCTCCCCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGttt                                                               >  1:290438/1‑149 (MQ=255)
                                                                                      gTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGttt                                                               >  1:1555171/1‑149 (MQ=255)
                                                                                      gTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGttt                                                               >  2:417702/1‑149 (MQ=255)
                                                                                         ccATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTc                                                            <  1:1309869/149‑1 (MQ=255)
                                                                                         ccATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTc                                                            <  1:935822/149‑1 (MQ=255)
                                                                                          cATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcg                                                           >  2:268372/1‑149 (MQ=255)
                                                                                           ctCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcgc                                                          <  1:325642/148‑1 (MQ=255)
                                                                                           aTCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcgc                                                          >  1:1514939/1‑149 (MQ=255)
                                                                                              agagGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGAt                                                       <  1:715725/149‑1 (MQ=255)
                                                                                               gagGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGAGCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATa                                                      >  1:325243/1‑149 (MQ=255)
                                                                                                agGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATaa                                                     <  1:576553/149‑1 (MQ=255)
                                                                                                agGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATaa                                                     <  2:899603/149‑1 (MQ=255)
                                                                                                agGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATaa                                                     <  1:48775/149‑1 (MQ=255)
                                                                                                  gCTACCGCCGCATTAAGCGCGGCAAAGGAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCCCCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTGCGCGATAagt                                                   >  1:890535/1‑149 (MQ=255)
                                                                                                   cTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAagta                                                  >  2:1242934/1‑149 (MQ=255)
                                                                                                     aCCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAagtagt                                                <  2:1446574/149‑1 (MQ=255)
                                                                                                      ccgccgCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAGCAGAATATAATTCACTGCTCCGCCAGTTTTTCTCGATAAGTAGTg                                               >  2:419727/1‑149 (MQ=255)
                                                                                                       cgccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTgg                                              >  2:774430/1‑149 (MQ=255)
                                                                                                       cgccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTgg                                              <  1:301850/149‑1 (MQ=255)
                                                                                                       cgccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTACCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTgg                                              >  2:706924/1‑149 (MQ=255)
                                                                                                        gccgcATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGc                                             <  2:18613/149‑1 (MQ=255)
                                                                                                            cATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAAATCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAAcc                                         >  1:484365/1‑149 (MQ=255)
                                                                                                              ttAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGa                                       >  1:1304826/1‑149 (MQ=255)
                                                                                                                aaGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGaaa                                     <  1:318406/149‑1 (MQ=255)
                                                                                                                aaGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGaaa                                     <  1:886160/149‑1 (MQ=255)
                                                                                                                 aGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAAc                                    <  1:1561810/149‑1 (MQ=255)
                                                                                                                      ggCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGt                               >  2:17070/1‑149 (MQ=255)
                                                                                                                      ggCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGt                               >  2:1543272/1‑149 (MQ=255)
                                                                                                                            gaagTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGaaaa                         >  1:1049854/1‑149 (MQ=255)
                                                                                                                              agTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATg                       <  1:528308/149‑1 (MQ=255)
                                                                                                                                 tGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCa                    <  1:863876/149‑1 (MQ=255)
                                                                                                                                    cATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATcc                 >  2:725617/1‑149 (MQ=255)
                                                                                                                                     aTTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGCACAGAAAATAATTCCCTTCTGCGCCAGTTTTTAGCGATAAGTATTGGCAACGGCAACTGAGTCGAAAATGGCATcaa                >  2:303692/1‑147 (MQ=255)
                                                                                                                                     aTTCCCCGGCACCACGGTGACGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAAGTCCCTGCTCCGCCAGTTTTTCGCTATAAGTAGTGGAAACAGAAACTGAGTCGTAAATGGCATCCa                >  2:299253/1‑149 (MQ=255)
                                                                                                                                      tatctcGGCACCGTGGTGCCGGGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCAc               >  1:726273/6‑149 (MQ=255)
                                                                                                                                         cccGGCACCACGTTGCCTAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGc            <  2:64984/149‑1 (MQ=255)
                                                                                                                                          tcGGCACCGTGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcg                                                           <  1:1081940/100‑1 (MQ=255)
                                                                                                                                          tcGGCACCGTGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTcg                                                           >  2:1081940/2‑101 (MQ=255)
                                                                                                                                            ggCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGAGCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCAc         <  2:836080/149‑1 (MQ=255)
                                                                                                                                                ccacGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACctct     <  1:527766/149‑1 (MQ=255)
                                                                                                                                                 cacGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGGGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCGCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCtt    <  2:1265809/149‑1 (MQ=255)
                                                                                                                                                   cGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTg  <  2:145853/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAAACTGCCCGGTTTTTTCTGCGTTAATGCGAAAATAGGTGGAAAGTTCCATCGGGCAGCGCACGCCTTTAGGCACATAAATAAACGTACCATCAGAGGCTACCGCCGCATTAAGCGCGGCAAAGAAGTTGTCATTCCCCGGCACCACGGTGCCGAGATATTTACGCACCAGTTCCGGGTGATCGTGGATCGCCTCACCAAAGGAACAGAAAATAATTCCCTGCTCCGCCAGTTTTTCGCGATAAGTAGTGGCAACCGAAACTGAGTCGAAAATGGCATCCACCGCCACCTCTTTG  >  NC_000913/1763304‑1763599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: