Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,787,558 C→G 5.7% I38M (ATC→ATG ppsR → PEP synthase kinase and PEP synthase pyrophosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,787,5580CG 5.7% 404.1 / 10.5 119I38M (ATC→ATGppsRPEP synthase kinase and PEP synthase pyrophosphorylase
Reads supporting (aligned to +/- strand):  ref base C (46/66);  new base G (2/5);  total (48/71)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGC  >  NC_000913/1787410‑1787705
                                                                                                                                                    |                                                                                                                                                   
tCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATc                                                                                                                                                     <  1:1354355/149‑1 (MQ=255)
    aGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGc                                                                                                                                                 <  2:146055/149‑1 (MQ=255)
     taaTTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCt                                                                                                                                                <  2:400309/148‑1 (MQ=255)
      aaTTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTg                                                                                                                                               >  2:432429/1‑149 (MQ=255)
       aTTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGc                                                                                                                                              <  1:653810/149‑1 (MQ=255)
       aTTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATAATTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGc                                                                                                                                              <  2:925193/149‑1 (MQ=255)
          aTGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCGTGAtgctgctga                                                                                                                                           <  1:297515/149‑4 (MQ=255)
          aTGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTACCCGTCACTATCAGCAGCGTGAtgctgctga                                                                                                                                           <  1:54742/149‑4 (MQ=255)
            gTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGATGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCGTGAtgctgctgata                                                                                                                                         <  2:1000632/149‑6 (MQ=255)
            gTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGttt                                                                                                                                         <  1:1097433/149‑1 (MQ=255)
              cTGCATCACGGGAAGAACAAAATGGATAATGATGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACAGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCGCTATCAGCAGCATCACGCTGCCGTTTGt                                                                                                                                       <  2:834026/149‑1 (MQ=255)
               tGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTc                                                                                                                                      <  1:51177/149‑1 (MQ=255)
               tGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTc                                                                                                                                      <  1:525267/149‑1 (MQ=255)
               tGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGAAGCATCACGCTGCCGTTTGTc                                                                                                                                      >  2:310602/1‑149 (MQ=255)
                  aTCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGaa                                                                                                                                   >  2:1565280/1‑149 (MQ=255)
                  aTCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGaa                                                                                                                                   <  1:253476/149‑1 (MQ=255)
                     aCGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAAt                                                                                                                                <  2:1472296/149‑1 (MQ=255)
                         gaagaaCAAAATGTATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATgaga                                                                                                                            <  1:1120900/149‑1 (MQ=255)
                         gaagaaCAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATgaga                                                                                                                            <  2:827864/149‑1 (MQ=255)
                           agaaCAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGc                                                                                                                          <  1:1213766/149‑1 (MQ=255)
                            gaaCAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGcc                                                                                                                         >  1:1184660/1‑149 (MQ=255)
                               cAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTg                                                                                                                      >  2:1396259/1‑149 (MQ=255)
                               cAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAAAGTCACAATTTCCCGTCACTATCAGAATCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTg                                                                                                                      >  2:243537/1‑149 (MQ=255)
                                 aaaTGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCa                                                                                                                    >  2:333831/1‑149 (MQ=255)
                                     ggATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACggg                                                                                                                <  2:540403/149‑1 (MQ=255)
                                      gATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCTGTTTGTCGAAAATGAGAGCCGTGCACGGGc                                                                                                               >  2:59829/1‑149 (MQ=255)
                                      gATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGc                                                                                                               >  1:1449596/1‑149 (MQ=255)
                                         aaTGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGt                                                                                                            >  1:862478/1‑149 (MQ=255)
                                         aaTGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCAAGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGt                                                                                                            >  1:1442895/1‑149 (MQ=255)
                                          aTGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTg                                                                                                           <  2:1369103/149‑1 (MQ=255)
                                           tGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGa                                                                                                          >  2:597335/1‑149 (MQ=255)
                                            gCTGTTGATCGCCTCGTTTTTTATATTTCTGATGGTACGGCACAAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAAATTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGaa                                                                                                         <  1:1486261/149‑1 (MQ=255)
                                             cTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAg                                                                                                        <  1:1312277/149‑1 (MQ=255)
                                              tGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAgg                                                                                                       <  1:117372/149‑1 (MQ=255)
                                              tGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAgg                                                                                                       >  1:7516/1‑149 (MQ=255)
                                               gTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGa                                                                                                      >  1:76304/1‑149 (MQ=255)
                                               gTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGa                                                                                                      <  1:118768/149‑1 (MQ=255)
                                                ttGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGAt                                                                                                     <  2:503095/149‑1 (MQ=255)
                                                 ttaTCGGCAGGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCATTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATc                                                                                                    <  1:553407/147‑1 (MQ=255)
                                                  gATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCTGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCa                                                                                                   <  2:1074260/149‑1 (MQ=255)
                                                   aTCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAg                                                                                                  >  1:302800/1‑149 (MQ=255)
                                                     cGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGAt                                                                                                <  1:150174/149‑1 (MQ=255)
                                                       ccACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTg                                                                                              >  1:434318/1‑149 (MQ=255)
                                                        cACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGa                                                                                             >  1:1084306/1‑149 (MQ=255)
                                                        cACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGa                                                                                             <  2:700161/149‑1 (MQ=255)
                                                         aCGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGAc                                                                                            <  1:1595355/149‑1 (MQ=255)
                                                         aCGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGAc                                                                                            <  2:1338555/149‑1 (MQ=255)
                                                         aCGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGAc                                                                                            <  2:484936/149‑1 (MQ=255)
                                                                    atTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCa                                                                                 <  2:179400/149‑1 (MQ=255)
                                                                    atTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCa                                                                                 <  1:1111698/149‑1 (MQ=255)
                                                                       tCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCa                                                                              >  1:937809/1‑149 (MQ=255)
                                                                       tCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCa                                                                              >  2:736262/1‑149 (MQ=255)
                                                                       tCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAACGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCa                                                                              <  2:671732/149‑1 (MQ=255)
                                                                       tCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTGTGTCGAAAAGGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCa                                                                              <  1:768346/149‑1 (MQ=255)
                                                                              ggaCGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACACTTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTTTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGAGCAGATTGACGCAATTTATCACCAGACAGGc                                                                       <  2:309685/147‑1 (MQ=255)
                                                                              gTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGc                                                                       <  2:135185/149‑1 (MQ=255)
                                                                               tACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCg                                                                      >  1:1297794/1‑149 (MQ=255)
                                                                               tACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCg                                                                      >  2:1198601/1‑149 (MQ=255)
                                                                                aCGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGt                                                                     <  2:577099/149‑1 (MQ=255)
                                                                                    cAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTgcgc                                                                 >  1:1028826/1‑149 (MQ=255)
                                                                                        aaCTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGc                                                             <  1:432429/149‑1 (MQ=255)
                                                                                         aCTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCt                                                            <  2:779080/149‑1 (MQ=255)
                                                                                          cTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTg                                                           >  2:1186648/1‑149 (MQ=255)
                                                                                          cTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTg                                                           <  2:1297794/149‑1 (MQ=255)
                                                                                          cTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTg                                                           <  1:507031/149‑1 (MQ=255)
                                                                                          cTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTg                                                           <  1:401639/149‑1 (MQ=255)
                                                                                          cTGCGGAGGTATTAGGACACGCAGTAATGTCACAATATCCCGGCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTg                                                           <  1:287592/149‑1 (MQ=255)
                                                                                           tGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTCCAAGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTgg                                                          >  2:1290386/1‑149 (MQ=255)
                                                                                            gCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGt                                                         <  2:1330202/149‑1 (MQ=255)
                                                                                                  gTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTa                                                   >  1:156043/1‑149 (MQ=255)
                                                                                                   tATTAGGACACGCAGTAATGTCACAATTTCCCGACACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTAc                                                  <  2:150579/149‑1 (MQ=255)
                                                                                                         gACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATc                                            <  1:538143/149‑1 (MQ=255)
                                                                                                          acacGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCg                                           <  2:626286/149‑1 (MQ=255)
                                                                                                              gCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGtt                                       >  2:772148/1‑149 (MQ=255)
                                                                                                              gCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGtt                                       <  1:597335/149‑1 (MQ=255)
                                                                                                              gCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGtt                                       >  2:62925/1‑149 (MQ=255)
                                                                                                              gCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGtt                                       >  1:652288/1‑149 (MQ=255)
                                                                                                               cAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCTGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTg                                      <  1:59829/149‑1 (MQ=255)
                                                                                                               cAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGGGCGCCCGCTGGTCTTCTACTCCATCGTGTTg                                      <  2:77086/149‑1 (MQ=255)
                                                                                                                agaaaTGTCACAATTTCCCGTCCCTATCAGCCGCAGCACGCTGCCGTTGGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGAGCAGATTGACGCAAGTTCTCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGc                                     <  1:298295/146‑1 (MQ=255)
                                                                                                                  tAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCg                                   >  2:560496/1‑149 (MQ=255)
                                                                                                                  tAATGTCACAATTTCCCGGCACTATCAGCAGCATCACGCTGCCGGTTGTCGAAAATGCGAGCCGTGCACGTGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCg                                   <  2:1393332/149‑1 (MQ=255)
                                                                                                                       tCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGAt                              >  1:439753/1‑149 (MQ=255)
                                                                                                                        cacaATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGAtt                             >  2:443430/1‑149 (MQ=255)
                                                                                                                         acaATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTc                            >  1:1183414/1‑149 (MQ=255)
                                                                                                                          caATTTCCCGTCACTATCATCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTcg                           <  2:287821/149‑1 (MQ=255)
                                                                                                                                cccGTCACTATCAGCCGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCCCGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTTCTCCATCGTGTTGCCGGAGATTCGCGCcat                     <  1:218200/149‑1 (MQ=255)
                                                                                                                                 ccGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCTCGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTACATCGTGTTGCCGGAGATTCGCGCcagc                    >  1:79142/1‑147 (MQ=255)
                                                                                                                                 ccGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCcatc                    >  1:1346995/1‑149 (MQ=255)
                                                                                                                                  cGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCcatca                   <  2:872677/149‑1 (MQ=255)
                                                                                                                                    tCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCatcatc                 <  1:1295264/149‑1 (MQ=255)
                                                                                                                                     cACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCt                <  1:772148/149‑1 (MQ=255)
                                                                                                                                      aCTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCtt               <  2:862478/149‑1 (MQ=255)
                                                                                                                                        aaacggcagcgTGATGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATc                                            >  2:305614/6‑118 (MQ=255)
                                                                                                                                        aaacggcagcgTGATGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATc                                            <  1:305614/113‑1 (MQ=255)
                                                                                                                                          tCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCaa           >  1:1493209/1‑149 (MQ=255)
                                                                                                                                             gcagcaTCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGt        >  2:531108/1‑149 (MQ=255)
                                                                                                                                             gcagcaTCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGt        >  1:334563/1‑149 (MQ=255)
                                                                                                                                                gcaTCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGaa     <  2:1028826/149‑1 (MQ=255)
                                                                                                                                                   tCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGc  >  1:283997/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAATGTCACAATTTCCCGTCACTATCAGCAGCATCACGCTGCCGTTTGTCGAAAATGAGAGCCGTGCACGGGCAGTGAAGGATCAGATTGACGCAATTTATCACCAGACAGGCGTGCGCCCGCTGGTCTTCTACTCCATCGTGTTGCCGGAGATTCGCGCCATCATCTTGCAAAGTGAAGGC  >  NC_000913/1787410‑1787705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: