Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,791,521 C→G 6.2% S167T (AGT→ACT)  ydiV ← anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,791,5210CG 6.2% 364.3 / 10.6 109S167T (AGT→ACT) ydiVanti‑FlhD4C2 factor, inactive EAL family phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (42/60);  new base G (3/4);  total (45/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 110 total aligned reads displayed.

ATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGG  >  NC_000913/1791373‑1791666
                                                                                                                                                    |                                                                                                                                                 
aTGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAAc                                                                                                                                                   >  1:1253402/1‑149 (MQ=255)
   gAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCTCACGAATAAACTGTTCGAATGAGCGGTGCGTGATTTGTTGCTGAAAAAAACttt                                                                                                                                                >  2:1012787/1‑149 (MQ=255)
     aaTCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTa                                                                                                                                              <  2:398185/149‑1 (MQ=255)
       tCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATc                                                                                                                                            <  1:1201352/149‑1 (MQ=255)
         gAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGTTGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCa                                                                                                                                          <  2:16416/149‑1 (MQ=255)
         gAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCa                                                                                                                                          <  2:476744/149‑1 (MQ=255)
               ggTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTAAATAAAACTTTTATCCAGCATGa                                                                                                                                    <  2:345454/149‑1 (MQ=255)
                 tAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGGATAAAAGTTTTATTCAGCaacaaa                                                                                                                                  <  1:563587/149‑6 (MQ=255)
                  aaTCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGAcgc                                                                                                                                 <  1:1071414/149‑1 (MQ=255)
                    tCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCgg                                                                                                                               <  1:776343/149‑1 (MQ=255)
                     atgtgCAAATATTTCTGCCGTATCAATGCCTCCCTCAATGATGCAATTGCAACAATGTGAAATTTGCGCCTGGATCGAACGAATAAACGGTTCGAAGGAGCTGTGCGTGATTTGTTTCTGAATAAAACTTTTATCCAGCATGACGCggg                                                                                                                              <  1:859651/148‑1 (MQ=255)
                         gCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATGAAACCTTTATCCAGCATGACGCGGGTGaa                                                                                                                          >  1:790819/1‑149 (MQ=255)
                         gCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGaa                                                                                                                          <  1:687244/149‑1 (MQ=255)
                         gCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGaa                                                                                                                          >  2:1538940/1‑149 (MQ=255)
                           cAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACTAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGaaaa                                                                                                                        >  2:169703/1‑149 (MQ=255)
                            aaTATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCTCACGAATAAACGGATCGAATGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATACAGCATGACGCGGGTGAAAAg                                                                                                                       >  1:315672/1‑149 (MQ=255)
                            aaTATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAg                                                                                                                       >  2:68151/1‑149 (MQ=255)
                             atatTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGAGCCTGGATCGCACGAATAAACGCTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGa                                                                                                                      >  1:231734/1‑149 (MQ=255)
                               atTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAcc                                                                                                                    <  1:1517533/149‑1 (MQ=255)
                               atTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAcc                                                                                                                    <  1:1513681/149‑1 (MQ=255)
                                 ttCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCAt                                                                                                                  <  2:1466100/149‑1 (MQ=255)
                                      ccGTATCAATGCCTCCCGCAATGCTGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACGATCtaaa                                                                                                             >  1:1509906/1‑149 (MQ=255)
                                       cGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCaaaaa                                                                                                            <  1:628142/149‑1 (MQ=255)
                                        gTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAAc                                                                                                           <  2:49693/149‑1 (MQ=255)
                                         tATCAATGCCTCCCGCAATGATACAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAAcc                                                                                                          >  1:1566874/1‑149 (MQ=255)
                                                gCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTTGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCa                                                                                                   <  1:579410/149‑1 (MQ=255)
                                                gCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCa                                                                                                   <  1:1487529/149‑1 (MQ=255)
                                                 ccTCCCGCAATGATGCAATTGCAACAAGGGGAATTTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGGGATTTGTTGATGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCAt                                                                                                  <  1:621203/149‑1 (MQ=255)
                                                 ccTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCAt                                                                                                  >  1:1573417/1‑149 (MQ=255)
                                                 ccTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCAt                                                                                                  >  1:1567395/1‑149 (MQ=255)
                                                  cTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCAtt                                                                                                 >  1:1123459/1‑149 (MQ=255)
                                                  cTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCAtt                                                                                                 <  2:1123459/149‑1 (MQ=255)
                                                   tCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATtg                                                                                                >  1:83518/1‑149 (MQ=255)
                                                     ccGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAACCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATtgtg                                                                                              >  2:333735/1‑149 (MQ=255)
                                                       gCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGCCCATCAAAAACCGCTTTCATTGTGCt                                                                                            >  1:1194472/1‑149 (MQ=255)
                                                         aaTGATGCAATTGCAACAAGCGGAAATTTGCGCCTGGATCGCACGAATAAACGGTGCGAAGGACCGGTGCGTGTTTTGTTGCTGAATAAAACTTTTATCCAGCATGAAGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTAt                                                                                          <  2:1160822/149‑1 (MQ=255)
                                                          atgatgCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTAtt                                                                                         <  2:403288/149‑1 (MQ=255)
                                                             atgCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTACGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGAGCTATTGcc                                                                                      >  1:1510324/1‑149 (MQ=255)
                                                               gCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTg                                                                                    <  2:425038/149‑1 (MQ=255)
                                                                   ttGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTcc                                                                                <  2:888012/149‑1 (MQ=255)
                                                                       aacaaGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCaaa                                                                            >  1:361935/1‑149 (MQ=255)
                                                                         caaGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAAtt                                                                          <  2:1253402/149‑1 (MQ=255)
                                                                         caaGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCGATTGCCTGCTCCCAAAtt                                                                          >  2:1407640/1‑149 (MQ=255)
                                                                            ggggAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTAcc                                                                       >  2:1564159/1‑149 (MQ=255)
                                                                              ggAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTa                                                                     >  2:1246831/1‑149 (MQ=255)
                                                                              ggAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTa                                                                     >  2:644198/1‑149 (MQ=255)
                                                                                    ttGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACaa                                                               >  1:345326/1‑149 (MQ=255)
                                                                                      gcgcCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAgg                                                             <  1:20619/149‑1 (MQ=255)
                                                                                          cTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCTTGACGCGGGTGAAAAGACCATCAAACACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGAt                                                         >  2:1414187/1‑149 (MQ=255)
                                                                                              ctCGCACGAATAAACGGTTCGAAGGAGCGGTGCGCGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATacac                                                     <  2:678505/148‑1 (MQ=255)
                                                                                                 gCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTg                                                  >  1:825786/1‑149 (MQ=255)
                                                                                                   aCGTATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCg                                                <  2:850823/149‑1 (MQ=255)
                                                                                                   aCGAATAAACGGTTCGAAGGAGCGGTGCGTGAGTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCg                                                <  2:155929/149‑1 (MQ=255)
                                                                                                    cGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGa                                               <  2:1215069/149‑1 (MQ=255)
                                                                                                     gAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGAt                                              >  2:460210/1‑149 (MQ=255)
                                                                                                     gAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGAt                                              >  2:429571/1‑149 (MQ=255)
                                                                                                     gAATAAACAGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAACCCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGTGTATCCCCTTgtgtt                                              <  1:68151/149‑5 (MQ=255)
                                                                                                       aTAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATaa                                            <  2:910851/149‑1 (MQ=255)
                                                                                                        tAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAg                                           <  2:1573417/149‑1 (MQ=255)
                                                                                                        tAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAg                                           <  2:1567395/149‑1 (MQ=255)
                                                                                                        tAAACGGTGCGAAGGAGCGGTGCGTGATTTGTTGCTGAATTAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCTTCAAAAACCTCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAg                                           <  1:96027/149‑1 (MQ=255)
                                                                                                          aaCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGa                                         <  1:1579340/149‑1 (MQ=255)
                                                                                                          aaCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGa                                         >  1:1495348/1‑149 (MQ=255)
                                                                                                           aCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGCCCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGAt                                        >  1:804994/1‑149 (MQ=255)
                                                                                                           aCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGAt                                        <  1:1569057/149‑1 (MQ=255)
                                                                                                            tggTTCGAAGGAGCTGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGGGGGTTAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTTCGATTAGtata                                       <  2:729520/148‑1 (MQ=255)
                                                                                                             ggTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATaa                                      >  1:1221206/1‑149 (MQ=255)
                                                                                                             ggTTAGAAGGAGCGGGGCGTGATGTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGTAAAGACCATCAAAAACCGCTTTGATTGGGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATaa                                      <  2:265789/149‑1 (MQ=255)
                                                                                                              gTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATaaa                                     >  1:1467059/1‑149 (MQ=255)
                                                                                                               ttCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATaaaa                                    <  2:1566874/149‑1 (MQ=255)
                                                                                                                  gAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGATTTCATTGTACTATTGCCTGCTCCCAAATTACATAACACAAGGGGATACACTTGCGATAAGGATAAAAGAc                                 >  1:998669/1‑149 (MQ=255)
                                                                                                                        gCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGTGGATACACTTGCGATAAGGATAAAAGACCGCGAt                           >  2:578380/1‑149 (MQ=255)
                                                                                                                        gCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGAt                           >  2:546862/1‑149 (MQ=255)
                                                                                                                        gCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATACAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGAt                           <  2:315672/149‑1 (MQ=255)
                                                                                                                             gCGTGATTTGTTGCTGAATGAAACCTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCt                      <  2:790819/149‑1 (MQ=255)
                                                                                                                              cGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCtt                     <  1:169703/149‑1 (MQ=255)
                                                                                                                               ttGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCttt                    <  2:487275/148‑1 (MQ=255)
                                                                                                                               gTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCttt                    <  2:718733/149‑1 (MQ=255)
                                                                                                                                 gATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTc                  <  1:428691/149‑1 (MQ=255)
                                                                                                                                    gtcatgCTGGATAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCg                                                <  2:606427/112‑1 (MQ=39)
                                                                                                                                    gtcatgCTGGATAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCg                                                >  1:606427/5‑116 (MQ=39)
                                                                                                                                        tgCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCAtt           <  1:217273/149‑1 (MQ=255)
                                                                                                                                          cTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTga         <  2:83518/149‑1 (MQ=255)
                                                                                                                                          cTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTga         >  2:1156677/1‑149 (MQ=255)
                                                                                                                                           tGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTgag        >  1:1238137/1‑149 (MQ=255)
                                                                                                                                           tGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTgag        >  1:504807/1‑149 (MQ=255)
                                                                                                                                           tGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTgag        <  2:1467059/149‑1 (MQ=255)
                                                                                                                                             aaTAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCACAAATTACCTAAAACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGAt      >  2:1432561/1‑149 (MQ=255)
                                                                                                                                              aTAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCt                                                                      >  1:669820/1‑84 (MQ=255)
                                                                                                                                              aTAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCt                                                                      >  1:675665/1‑84 (MQ=255)
                                                                                                                                              aTAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCt                                                                      <  2:669820/84‑1 (MQ=255)
                                                                                                                                               tAAAACTTTTATCCAGCATGACGCGGGTGAAAAGCCCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAAGTACCTAACACAAGGAGATACACTTGCGATAAGGATAAAAGACCGCTATTATCTTTTCCTTCATTGAGATGc    >  2:938661/1‑149 (MQ=255)
                                                                                                                                               tAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCTTCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGc    <  2:13279/149‑1 (MQ=255)
                                                                                                                                               tAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGc    <  1:386270/149‑1 (MQ=255)
                                                                                                                                                 aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAGGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg  <  1:770158/149‑1 (MQ=255)
                                                                                                                                                 aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg  <  1:1538940/149‑1 (MQ=255)
                                                                                                                                                 aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg  >  2:301110/1‑149 (MQ=255)
                                                                                                                                                 aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg  >  2:396609/1‑149 (MQ=255)
                                                                                                                                                 aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg  <  1:1353716/149‑1 (MQ=255)
                                                                                                                                                 aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg  <  2:466686/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ATGGAAATCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGG  >  NC_000913/1791373‑1791666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: