Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 314,573 G→C 6.5% pseudogene (217/872 nt) eaeH → pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913314,5730GC 6.5% 303.6 / 10.3 93pseudogene (217/872 nt)eaeHpseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base G (34/52);  new base C (3/3);  total (38/55)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

GTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACT  >  NC_000913/314425‑314721
                                                                                                                                                    |                                                                                                                                                    
gtggGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTcg                                                                                                                                                      <  2:476970/149‑1 (MQ=255)
gtggGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTcg                                                                                                                                                      <  2:1322711/149‑1 (MQ=255)
gtggGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTcg                                                                                                                                                      <  2:1162845/149‑1 (MQ=255)
  gggCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTcgcg                                                                                                                                                    >  2:670290/1‑149 (MQ=255)
    gCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGcgtc                                                                                                                                                  >  1:681544/1‑149 (MQ=255)
    gCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGcgtc                                                                                                                                                  >  2:178404/1‑149 (MQ=255)
         tatCTCTGTTCAGGGTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTg                                                                                                                                             <  2:233081/149‑1 (MQ=255)
           tctcTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGcc                                                                                                                                           <  2:1342435/149‑1 (MQ=255)
            ctctGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCg                                                                                                                                          <  2:843660/149‑1 (MQ=255)
            ctctGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCg                                                                                                                                          >  2:653597/1‑149 (MQ=255)
             tctGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGc                                                                                                                                         >  1:1383739/1‑149 (MQ=255)
               tGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACAGCTGATAATAACTTGGAGAAAAAAGTCTCGTCCTTTGCCGCaa                                                                                                                                       >  2:1517373/1‑149 (MQ=255)
                gTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCaaa                                                                                                                                      <  1:159964/149‑1 (MQ=255)
                  tCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCaaagg                                                                                                                                    >  1:1535897/1‑147 (MQ=255)
                    aGGTTCTTTTTCCACTCGCTGTCCCCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCAGGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGcc                                                                                                                                  >  2:1474158/1‑149 (MQ=255)
                     ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGTACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  2:418972/149‑1 (MQ=255)
                     ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  2:492040/149‑1 (MQ=255)
                     ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  1:1388257/149‑1 (MQ=255)
                     ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  1:1266803/149‑1 (MQ=255)
                     ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  1:872171/149‑1 (MQ=255)
                     ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  1:25567/149‑1 (MQ=255)
                      gTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACTGTAACTGCTGATAATAACGTGGAGAAAAATGACGCGTCGTTTGCCGCAAATGCCgg                                                                                                                                >  2:978269/1‑149 (MQ=255)
                       ttCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATTACGTGGAGATAAATGTCGCGTCGTTTGCCGCTTATGCCggg                                                                                                                               >  1:629261/1‑149 (MQ=255)
                       ttCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCggg                                                                                                                               >  2:1586033/1‑149 (MQ=255)
                        tCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGa                                                                                                                              <  2:1528702/149‑1 (MQ=255)
                        tCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGa                                                                                                                              <  1:598328/149‑1 (MQ=255)
                         cTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACTTCGAGAAAAATGTCGCGTCGTATGCCGCAAATGCCGGGAc                                                                                                                             >  2:248610/1‑149 (MQ=255)
                            tttCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTGCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTGTGCCGCAAATGCCGGGACAtt                                                                                                                          <  2:641643/149‑1 (MQ=255)
                             ttCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACAttt                                                                                                                         <  2:598084/149‑1 (MQ=255)
                               ccACTCGCTGTCCCCTTTACCCCAGTAATGGCGGCACTTGCGCAGCATGCGGGTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAttttt                                                                                                                       <  2:1149062/149‑1 (MQ=37)
                               ccACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAttttt                                                                                                                       <  2:1494512/149‑1 (MQ=38)
                                cACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAtttttc                                                                                                                      <  2:527536/149‑2 (MQ=38)
                                 aCTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTaa                                                                                                                     <  2:1444087/149‑1 (MQ=255)
                                  cTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                    <  2:1535897/149‑1 (MQ=255)
                                  cTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                    >  2:1227641/1‑149 (MQ=255)
                                  cTCGCTGGCACCTTTACCCCAGAAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                    <  1:365269/149‑1 (MQ=255)
                                    cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCa                                                                                                                  <  1:670290/149‑1 (MQ=255)
                                    cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCa                                                                                                                  <  2:653514/149‑1 (MQ=255)
                                    cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCa                                                                                                                  <  1:1517673/149‑1 (MQ=255)
                                           aCCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGcc                                                                                                           <  2:681544/149‑1 (MQ=255)
                                           aCCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGcc                                                                                                           >  2:1282721/1‑149 (MQ=255)
                                            ccTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGCGCAGCCACGGGTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCa                                                                                                          <  1:930139/149‑1 (MQ=255)
                                                tACCCCAGTAATGGCGGCACGGGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATa                                                                                                      <  2:169092/149‑1 (MQ=255)
                                                   cccAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCg                                                                                                   >  1:1463218/1‑149 (MQ=255)
                                                   cccAGTAATGGCGGCACGTGCGCAGCATGCGGTTAAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCg                                                                                                   >  2:958529/1‑149 (MQ=255)
                                                     cAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                 <  1:667921/149‑1 (MQ=255)
                                                     cAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                 <  1:1224959/149‑1 (MQ=255)
                                                     cAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                 >  1:1472424/1‑149 (MQ=255)
                                                      aGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATg                                                                                                <  1:972749/149‑1 (MQ=255)
                                                       gTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGc                                                                                               <  1:1257966/149‑1 (MQ=255)
                                                         aaTGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGa                                                                                             <  2:665147/149‑1 (MQ=255)
                                                                gcACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCCTGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTaa                                                                                      <  1:1267052/149‑1 (MQ=255)
                                                                   agTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGTGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACtt                                                                                   <  2:868142/148‑1 (MQ=255)
                                                                     tGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATTACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGGCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                 <  2:229981/149‑1 (MQ=255)
                                                                     tGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTACTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                 >  1:1273781/1‑149 (MQ=255)
                                                                          agcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTAcc                                                                            >  2:815561/1‑149 (MQ=255)
                                                                          agcaTGCGGCTCAGCCACGGTGGAGCATGGGAAATACTACTGTAACTGCTGATAATAACGTGGAGAAATCTGTCGCGTCGTTTGCCGCAAATGCCGGGACATGTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTAcc                                                                            <  1:982015/149‑1 (MQ=255)
                                                                           gcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCg                                                                           >  2:106277/1‑149 (MQ=255)
                                                                           gcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCg                                                                           <  1:1297517/149‑1 (MQ=255)
                                                                                 ggTGCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATgg                                                                     <  2:569508/149‑1 (MQ=255)
                                                                                  gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGc                                                                    >  2:400137/1‑149 (MQ=255)
                                                                                  gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGc                                                                    >  2:795635/1‑149 (MQ=255)
                                                                                  gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGc                                                                    >  1:1230415/1‑149 (MQ=255)
                                                                                    tctgCCACGGTTGAGCATTGGAAATAATACTGTAAATGCTTATTATAACGTGGAGAAAAATGTAGAGTCGGTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCca                                                                  <  2:1430818/146‑1 (MQ=255)
                                                                                             gTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACagctaaag                                                         <  2:187128/149‑1 (MQ=255)
                                                                                                  gCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAAc                                                    <  2:1382062/149‑1 (MQ=255)
                                                                                                   cATGGGAATTACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCAACAGCTAAAGCTAAcc                                                   >  2:1451210/1‑149 (MQ=255)
                                                                                                    aTGGGAAATACTACGGTAACTGCTGATAATTACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTTCCGGAATGGCCACAGCTAAAGCTAACCa                                                  <  2:1570621/149‑1 (MQ=255)
                                                                                                    aTGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCa                                                  <  2:923534/149‑1 (MQ=255)
                                                                                                     tGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAg                                                 <  1:1576907/149‑1 (MQ=255)
                                                                                                         aaaTACTACGGTAACTGATGATAATAACGTCGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACATCTAAAGCTAACCAGGaaa                                             <  1:1517373/149‑1 (MQ=255)
                                                                                                            tactacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATAc                                          >  1:1496565/1‑149 (MQ=255)
                                                                                                             actacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCCTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACa                                         <  2:322192/149‑1 (MQ=255)
                                                                                                              ctacTGTAACTGCTGATAATAAAGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAg                                        <  2:491561/149‑1 (MQ=255)
                                                                                                               tacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                 <  1:1052437/107‑1 (MQ=255)
                                                                                                               tacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                 >  2:1052437/1‑107 (MQ=255)
                                                                                                                acGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGa                                      <  1:653597/149‑1 (MQ=255)
                                                                                                                   gTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTg                                   >  2:1343454/1‑149 (MQ=255)
                                                                                                                     aaCTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGAGATGAGACACGTAACTGTATTACCGGAATGGCCACAGCTAAAGCTATCGAGGAATTACAGGAGTgga                                 >  2:207436/1‑148 (MQ=255)
                                                                                                                       ctgctgATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTc                               <  2:1545100/149‑1 (MQ=255)
                                                                                                                        tgctgATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCg                              >  1:605513/1‑149 (MQ=255)
                                                                                                                             ataataACGTGGAGAAAAATGTCGCGTCGTTTGACGCAAATGACGGGACATTTTTAAGAAGACAGCCAGATAGCGATGCGACTCGTAACTTTATTACCTGAATGTAAACAGCTAAATCTAAACAGTAAATACATTAGTGGATCGGTaaa                         >  1:988973/1‑149 (MQ=255)
                                                                                                                               aataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGAGATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTAATTACCGGAATGGCCACAGCTAAAGCTAACAAGGAAATACAGGAGAGGCTCGGGAAata                       >  2:609302/1‑149 (MQ=255)
                                                                                                                               aataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAata                       <  1:178404/149‑1 (MQ=255)
                                                                                                                                ataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAatat                      >  1:1051050/1‑149 (MQ=255)
                                                                                                                                 taaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATg                     <  1:10704/149‑1 (MQ=255)
                                                                                                                                    cGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTTGGGAAATATGGTa                  <  1:106277/149‑1 (MQ=255)
                                                                                                                                    cGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTa                  <  2:1155907/149‑1 (MQ=255)
                                                                                                                                     gTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTAc                 >  2:609088/1‑149 (MQ=255)
                                                                                                                                      tgcttaaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACt                >  1:19371/6‑149 (MQ=37)
                                                                                                                                         ttaaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcg             >  2:484492/3‑149 (MQ=38)
                                                                                                                                           aaaaaTGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAAGTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCACGGGAAATATGGTACTgcgcg           >  2:875986/1‑149 (MQ=255)
                                                                                                                                           aaaaaTGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcgcg           >  1:553622/1‑149 (MQ=255)
                                                                                                                                           aaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcgcg           >  2:721573/1‑149 (MQ=38)
                                                                                                                                                 gTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCaa     >  2:1376189/1‑149 (MQ=255)
                                                                                                                                                  tCGCGTCGTTTGCCGCAAATGCCGGTACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCaaa    <  1:978269/149‑1 (MQ=255)
                                                                                                                                                  tCGCGTCGTTTGCAGCAAATTCCGGGACATTATTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCaaa    <  2:817048/149‑1 (MQ=255)
                                                                                                                                                    gcgTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACt  >  1:594956/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACT  >  NC_000913/314425‑314721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: