Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,791,528 C→T 6.4% D165N (GAT→AAT)  ydiV ← anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,791,5280CT 6.4% 339.7 / 7.2 110D165N (GAT→AAT) ydiVanti‑FlhD4C2 factor, inactive EAL family phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (44/57);  new base T (3/4);  total (48/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 112 total aligned reads displayed.

TCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCA  >  NC_000913/1791380‑1791676
                                                                                                                                                    |                                                                                                                                                    
tCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATc                                                                                                                                                      <  1:1201352/149‑1 (MQ=255)
  gAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGTTGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCa                                                                                                                                                    <  2:16416/149‑1 (MQ=255)
  gAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCa                                                                                                                                                    <  2:476744/149‑1 (MQ=255)
   aaGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAg                                                                                                                                                   <  2:144858/149‑1 (MQ=255)
          tAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGGATAAAAGTTTTATTCAGCaacaaa                                                                                                                                            <  1:563587/149‑6 (MQ=255)
           aaTCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGAcgc                                                                                                                                           <  1:1071414/149‑1 (MQ=255)
              atgtgCAAATATTTCTGCCGTATCAATGCCTCCCTCAATGATGCAATTGCAACAATGTGAAATTTGCGCCTGGATCGAACGAATAAACGGTTCGAAGGAGCTGTGCGTGATTTGTTTCTGAATAAAACTTTTATCCAGCATGACGCggg                                                                                                                                        <  1:859651/148‑1 (MQ=255)
                  gCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATGAAACCTTTATCCAGCATGACGCGGGTGaa                                                                                                                                    >  1:790819/1‑149 (MQ=255)
                  gCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGaa                                                                                                                                    >  2:1538940/1‑149 (MQ=255)
                  gCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGaa                                                                                                                                    <  1:687244/149‑1 (MQ=255)
                    cAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACTAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGaaaa                                                                                                                                  >  2:169703/1‑149 (MQ=255)
                    cAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGaaaa                                                                                                                                  <  1:725595/149‑1 (MQ=255)
                     aaTATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCTCACGAATAAACGGATCGAATGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATACAGCATGACGCGGGTGAAAAg                                                                                                                                 >  1:315672/1‑149 (MQ=255)
                      atatTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGAGCCTGGATCGCACGAATAAACGCTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGa                                                                                                                                >  1:231734/1‑149 (MQ=255)
                        atTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAcc                                                                                                                              <  1:1513681/149‑1 (MQ=255)
                        atTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGAcc                                                                                                                              <  1:1517533/149‑1 (MQ=255)
                          ttCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCAt                                                                                                                            <  2:1466100/149‑1 (MQ=255)
                               ccGTATCAATGCCTCCCGCAATGCTGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACGATCtaaa                                                                                                                       >  1:1509906/1‑149 (MQ=255)
                                cGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCaaaaa                                                                                                                      <  1:628142/149‑1 (MQ=255)
                                 gTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAAc                                                                                                                     <  2:49693/149‑1 (MQ=255)
                                  tATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAAcc                                                                                                                    <  2:1048763/149‑1 (MQ=255)
                                  tATCAATGCCTCCCGCAATGATACAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAAcc                                                                                                                    >  1:1566874/1‑149 (MQ=255)
                                         gCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTTGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCa                                                                                                             <  1:579410/149‑1 (MQ=255)
                                          ccTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCAt                                                                                                            >  1:1573417/1‑149 (MQ=255)
                                           cTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCAtt                                                                                                           <  2:1123459/149‑1 (MQ=255)
                                           cTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCAtt                                                                                                           >  1:1123459/1‑149 (MQ=255)
                                            tCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATtg                                                                                                          >  1:83518/1‑149 (MQ=255)
                                              ccGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAACCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATtgtg                                                                                                        >  2:333735/1‑149 (MQ=255)
                                                gCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGCCCATCAAAAACCGCTTTCATTGTGCt                                                                                                      >  1:1194472/1‑149 (MQ=255)
                                                  aaTGATGCAATTGCAACAAGCGGAAATTTGCGCCTGGATCGCACGAATAAACGGTGCGAAGGACCGGTGCGTGTTTTGTTGCTGAATAAAACTTTTATCCAGCATGAAGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTAt                                                                                                    <  2:1160822/149‑1 (MQ=255)
                                                   atgatgCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTAtt                                                                                                   <  2:403288/149‑1 (MQ=255)
                                                      atgCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTACGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGAGCTATTGcc                                                                                                >  1:1510324/1‑149 (MQ=255)
                                                        gCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTg                                                                                              <  2:425038/149‑1 (MQ=255)
                                                            ttGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTcc                                                                                          <  2:888012/149‑1 (MQ=255)
                                                                aacaaGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCaaa                                                                                      >  1:361935/1‑149 (MQ=255)
                                                                  caaGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAAtt                                                                                    <  2:1253402/149‑1 (MQ=255)
                                                                  caaGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCGATTGCCTGCTCCCAAAtt                                                                                    >  2:1407640/1‑149 (MQ=255)
                                                                     ggggAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTAcc                                                                                 <  1:458141/149‑1 (MQ=255)
                                                                     ggggAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTAcc                                                                                 >  2:1564159/1‑149 (MQ=255)
                                                                       ggAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTa                                                                               >  2:1246831/1‑149 (MQ=255)
                                                                       ggAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTa                                                                               >  2:644198/1‑149 (MQ=255)
                                                                               gcgcCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAgg                                                                       <  1:20619/149‑1 (MQ=255)
                                                                                       ctCGCACGAATAAACGGTTCGAAGGAGCGGTGCGCGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATacac                                                               <  2:678505/148‑1 (MQ=255)
                                                                                          gCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTg                                                            >  1:825786/1‑149 (MQ=255)
                                                                                            aCGTATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCg                                                          <  2:850823/149‑1 (MQ=255)
                                                                                            aCGAATAAACGGTTCGAAGGAGCGGTGCGTGAGTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCg                                                          <  2:155929/149‑1 (MQ=255)
                                                                                             cGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGa                                                         <  2:1215069/149‑1 (MQ=255)
                                                                                              gAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGAt                                                        >  2:429571/1‑149 (MQ=255)
                                                                                              gAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGAt                                                        >  2:460210/1‑149 (MQ=255)
                                                                                              gAATAAACAGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAACCCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGTGTATCCCCTTgtgtt                                                        <  1:68151/149‑5 (MQ=255)
                                                                                                aTAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATaa                                                      <  2:910851/149‑1 (MQ=255)
                                                                                                 tAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAg                                                     <  2:1573417/149‑1 (MQ=255)
                                                                                                 tAAACGGTGCGAAGGAGCGGTGCGTGATTTGTTGCTGAATTAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCTTCAAAAACCTCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAg                                                     <  1:96027/149‑1 (MQ=255)
                                                                                                   aaCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGa                                                   >  1:1495348/1‑149 (MQ=255)
                                                                                                   aaCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGa                                                   <  1:1579340/149‑1 (MQ=255)
                                                                                                    aCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGCCCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGAt                                                  >  1:804994/1‑149 (MQ=255)
                                                                                                    aCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGAt                                                  <  1:1569057/149‑1 (MQ=255)
                                                                                                    aCGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACGTGCGATAAGGAt                                                  >  2:22060/1‑149 (MQ=255)
                                                                                                     tggTTCGAAGGAGCTGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGGGGGTTAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTTCGATTAGtata                                                 <  2:729520/148‑1 (MQ=255)
                                                                                                      ggTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATaa                                                >  1:1221206/1‑149 (MQ=255)
                                                                                                      ggTTAGAAGGAGCGGGGCGTGATGTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGTAAAGACCATCAAAAACCGCTTTGATTGGGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATaa                                                <  2:265789/149‑1 (MQ=255)
                                                                                                       gTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATaaa                                               >  1:1467059/1‑149 (MQ=255)
                                                                                                        ttCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATaaaa                                              <  2:1566874/149‑1 (MQ=255)
                                                                                                                 gCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGTGGATACACTTGCGATAAGGATAAAAGACCGCGAt                                     >  2:578380/1‑149 (MQ=255)
                                                                                                                 gCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGAt                                     >  2:546862/1‑149 (MQ=255)
                                                                                                                 gCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATACAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGAt                                     <  2:315672/149‑1 (MQ=255)
                                                                                                                      gCGTGATTTGTTGCTGAATGAAACCTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCt                                <  2:790819/149‑1 (MQ=255)
                                                                                                                       cGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCtt                               <  1:169703/149‑1 (MQ=255)
                                                                                                                        gTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCttt                              <  2:718733/149‑1 (MQ=255)
                                                                                                                          gATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTc                            <  1:428691/149‑1 (MQ=255)
                                                                                                                             gtcatgCTGGATAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCg                                                          <  2:606427/112‑1 (MQ=39)
                                                                                                                             gtcatgCTGGATAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCg                                                          >  1:606427/5‑116 (MQ=39)
                                                                                                                               gttgCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCa                       <  2:430190/149‑1 (MQ=255)
                                                                                                                                 tgCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCAtt                     <  1:217273/149‑1 (MQ=255)
                                                                                                                                   cTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTga                   >  2:1156677/1‑149 (MQ=255)
                                                                                                                                   cTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTga                   <  2:83518/149‑1 (MQ=255)
                                                                                                                                    tGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTgag                  >  1:1238137/1‑149 (MQ=255)
                                                                                                                                    tGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTgag                  <  2:1467059/149‑1 (MQ=255)
                                                                                                                                    tGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTgag                  >  1:504807/1‑149 (MQ=255)
                                                                                                                                      aaTAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGAt                >  1:1495240/1‑149 (MQ=255)
                                                                                                                                      aaTAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCACAAATTACCTAAAACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGAt                >  2:1432561/1‑149 (MQ=255)
                                                                                                                                       aTAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCt                                                                                >  1:669820/1‑84 (MQ=255)
                                                                                                                                       aTAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCt                                                                                >  1:675665/1‑84 (MQ=255)
                                                                                                                                       aTAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCt                                                                                <  2:675665/84‑1 (MQ=255)
                                                                                                                                       aTAAAAGTTTTATTCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCt                                                                                <  2:669820/84‑1 (MQ=255)
                                                                                                                                        tAAAACTTTTATCCAGCATGACGCGGGTGAAAAGCCCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAAGTACCTAACACAAGGAGATACACTTGCGATAAGGATAAAAGACCGCTATTATCTTTTCCTTCATTGAGATGc              >  2:938661/1‑149 (MQ=255)
                                                                                                                                        tAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCTTCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGc              <  2:13279/149‑1 (MQ=255)
                                                                                                                                        tAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGc              <  1:386270/149‑1 (MQ=255)
                                                                                                                                          aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAGGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg            <  1:770158/149‑1 (MQ=255)
                                                                                                                                          aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg            <  1:1353716/149‑1 (MQ=255)
                                                                                                                                          aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg            >  2:301110/1‑149 (MQ=255)
                                                                                                                                          aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg            <  1:1538940/149‑1 (MQ=255)
                                                                                                                                          aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg            <  2:466686/149‑1 (MQ=255)
                                                                                                                                          aaaCTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCgg            >  2:396609/1‑149 (MQ=255)
                                                                                                                                              ttttATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTaa        <  2:361935/149‑1 (MQ=255)
                                                                                                                                              ttttATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTaa        >  1:393073/1‑149 (MQ=255)
                                                                                                                                                  aTCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCCTTTCCTTCATTGAGATGCGGGTAATtct    >  1:896669/1‑149 (MQ=255)
                                                                                                                                                   tCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATtctc   >  1:896050/1‑149 (MQ=255)
                                                                                                                                                   tCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATtctc   >  1:710079/1‑149 (MQ=255)
                                                                                                                                                    ccAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCATTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCa  <  1:1012787/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCGAAGGGGGTAATCTGTGCCAATATTTCTGCCGTATCAATGCCTCCCGCAATGATGCAATTGCAACAAGGGGAAATTTGCGCCTGGATCGCACGAATAAACGGTTCGAAGGAGCGGTGCGTGATTTGTTGCTGAATAAAACTTTTATCCAGCATGACGCGGGTGAAAAGACCATCAAAAACCGCTTTCATTGTGCTATTGCCTGCTCCCAAATTACCTAACACAAGGGGATACACTTGCGATAAGGATAAAAGACCGCGATTATCTTTTCCTTCATTGAGATGCGGGTAATTCTCA  >  NC_000913/1791380‑1791676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: