Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,821,241 T→A 6.2% intergenic (‑27/+58) chbC ← / ← chbB N,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS/N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,821,2410TA 6.2% 482.5 / 13.9 156intergenic (‑27/+58)chbC/chbBN,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS/N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base T (73/73);  new base A (7/3);  total (80/76)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 160 total aligned reads displayed.

GCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTA  >  NC_000913/1821093‑1821387
                                                                                                                                                     |                                                                                                                                                  
gCAAGGGTTAACTGCATTAAGCGAATATAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTCCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAAtt                                                                                                                                                    <  2:1087675/149‑1 (MQ=255)
gCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTATTGATTGCCTTAACGAGTGGCTGCGCTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAAtt                                                                                                                                                    <  2:776101/149‑1 (MQ=255)
 cAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCTATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAAttt                                                                                                                                                   >  2:456314/1‑149 (MQ=255)
    gggTTAACGGCATTACGCGAATTAATCCATTTTTGATTGCATTAACGTGTGGCTTCTTTCCTAATTAAACTGCAAAAGGGAGGAGTACCATTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTaaa                                                                                                                                                <  2:1498389/149‑1 (MQ=255)
    gggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTaaa                                                                                                                                                <  1:2372/149‑1 (MQ=255)
    gggTTAACGGCATTAAGCGAATAAAGACATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTGTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTaaa                                                                                                                                                <  2:1222764/149‑1 (MQ=255)
      gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAata                                                                                                                                              >  1:102605/1‑149 (MQ=255)
      gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAata                                                                                                                                              >  2:828023/1‑149 (MQ=255)
      gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAata                                                                                                                                              >  1:68901/1‑149 (MQ=255)
      gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAata                                                                                                                                              <  1:403565/149‑1 (MQ=255)
         aaCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTg                                                                                                                                           <  1:575748/149‑1 (MQ=255)
             gCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTAAAATATTGCGGt                                                                                                                                       >  2:745738/1‑149 (MQ=255)
             gCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATATTTAAATTGCGGt                                                                                                                                       <  2:1058579/149‑1 (MQ=255)
             gCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTa                                                                                                                                      >  2:1091052/1‑149 (MQ=255)
              cATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTa                                                                                                                                      <  2:1046640/149‑1 (MQ=255)
                ttAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTAtt                                                                                                                                    >  2:1426680/1‑149 (MQ=255)
                ttAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTAtt                                                                                                                                    >  2:1257880/1‑149 (MQ=255)
                   aGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGAt                                                                                                                                 <  2:505201/149‑1 (MQ=255)
                   aGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGAt                                                                                                                                 >  1:1128520/1‑149 (MQ=255)
                    gCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGAtt                                                                                                                                >  2:1135134/1‑149 (MQ=255)
                     cGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATATTTAAATTGCGGtttttaagg                                                                                                                               <  1:809463/149‑6 (MQ=255)
                      gAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTa                                                                                                                              >  2:124873/1‑149 (MQ=255)
                       aaTAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTAt                                                                                                                             <  2:614346/149‑1 (MQ=255)
                        aTAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGAAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGAGGTATTGATTTATg                                                                                                                            >  1:1045024/1‑149 (MQ=255)
                           aaGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGaa                                                                                                                          >  2:768081/1‑149 (MQ=255)
                           aaGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGaaa                                                                                                                         <  1:452454/149‑1 (MQ=255)
                           aaGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGaaa                                                                                                                         <  2:102605/149‑1 (MQ=255)
                           aaGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGaaa                                                                                                                         >  2:1013438/1‑149 (MQ=255)
                            aGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGTTATTGATTTATGAAAt                                                                                                                        >  1:292757/1‑149 (MQ=255)
                            aGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGaaaa                                                                                                                        >  1:507066/1‑148 (MQ=255)
                            aGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAAt                                                                                                                        >  1:858467/1‑149 (MQ=255)
                             gCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATa                                                                                                                       >  2:2442/1‑149 (MQ=255)
                             gCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATa                                                                                                                       >  2:545380/1‑149 (MQ=255)
                                    ttGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCttt                                                                                                                <  2:582925/149‑1 (MQ=255)
                                      gATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGa                                                                                                              <  1:1223679/149‑1 (MQ=255)
                                        ttGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACg                                                                                                            <  1:496697/149‑1 (MQ=255)
                                           cATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGa                                                                                                         >  1:468223/1‑149 (MQ=255)
                                                aCGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAAtt                                                                                                    >  2:9308/1‑149 (MQ=255)
                                                aCGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAAtt                                                                                                    >  2:68172/1‑149 (MQ=255)
                                                aCGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAAtt                                                                                                    >  1:672408/1‑149 (MQ=255)
                                                    gtgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAGCTCTTTGACGGGAAAATTTaaa                                                                                                >  1:935533/1‑149 (MQ=255)
                                                     tgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaaa                                                                                               <  1:545380/149‑1 (MQ=255)
                                                       gctgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaata                                                                                             >  2:565736/1‑149 (MQ=255)
                                                        ctgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataa                                                                                            >  1:1022422/1‑149 (MQ=255)
                                                         tgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaa                                                                                           <  2:127049/149‑1 (MQ=255)
                                                         tgctTTACTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaa                                                                                           <  1:1084807/149‑1 (MQ=255)
                                                            tttCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaatta                                                                                        >  2:1177248/1‑149 (MQ=255)
                                                              tCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaat                                                                                      <  2:630248/149‑1 (MQ=255)
                                                                  aTTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgc                                                                                  <  1:380770/149‑1 (MQ=255)
                                                                        aCTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGc                                                                            >  1:830814/1‑149 (MQ=255)
                                                                          tGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTTCGGCtt                                                                          >  2:173581/1‑149 (MQ=255)
                                                                          tGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtt                                                                          <  1:69050/149‑1 (MQ=255)
                                                                             aaaaGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCttttt                                                                       >  1:1092694/1‑149 (MQ=255)
                                                                              aaaGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACTGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttttt                                                                      <  2:1554183/149‑1 (MQ=255)
                                                                              aaaGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttttt                                                                      <  1:1512155/149‑1 (MQ=255)
                                                                                 gggAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTaa                                                                    <  1:768081/149‑1 (MQ=255)
                                                                                    aggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGc                                                                >  2:717396/1‑149 (MQ=255)
                                                                                     ggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCt                                                               <  1:1135134/149‑1 (MQ=255)
                                                                                     ggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCt                                                               <  2:293658/149‑1 (MQ=255)
                                                                                      gagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGTAAAATTTAAAATAAAATAATTTGCTGCGGCTTTTTTAATCGCTg                                                              >  2:107374/1‑149 (MQ=255)
                                                                                      gagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTg                                                              >  2:927007/1‑149 (MQ=255)
                                                                                      gagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTg                                                              <  2:313241/149‑1 (MQ=255)
                                                                                      gagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTg                                                              >  2:97823/1‑149 (MQ=255)
                                                                                        gTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTaa                                                                    <  2:64479/141‑1 (MQ=255)
                                                                                        gTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTaa                                                                    >  1:64479/1‑141 (MQ=255)
                                                                                         tACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                           >  2:475143/1‑149 (MQ=255)
                                                                                         tACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                           >  2:1577747/1‑149 (MQ=255)
                                                                                            cTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAAATTAAATATTGCGGTATTGATTTATGAAATAACTATTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGTCTTTTTTAATCGCTGCAACCg                                                        >  1:309756/1‑149 (MQ=255)
                                                                                                  aaGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTTCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGcat                                                  >  2:1488805/1‑147 (MQ=255)
                                                                                                      gATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAg                                              <  2:507066/149‑1 (MQ=255)
                                                                                                       aTGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGc                                             >  1:419664/1‑149 (MQ=255)
                                                                                                         gCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCAc                                           <  1:401520/149‑1 (MQ=255)
                                                                                                           aaTAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGc                                         >  1:674389/1‑149 (MQ=255)
                                                                                                                cATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTaaa                                    <  1:1257880/149‑1 (MQ=255)
                                                                                                                 aTTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAAc                                   <  2:254662/149‑1 (MQ=255)
                                                                                                                       cATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGa                             >  2:837087/1‑149 (MQ=255)
                                                                                                                        aTAGAAAGATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGAc                            >  2:1363328/1‑149 (MQ=255)
                                                                                                                        aTAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                           >  2:394202/1‑118 (MQ=255)
                                                                                                                        aTAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGAc                            <  1:782212/149‑1 (MQ=255)
                                                                                                                        aTAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTATGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTTAACCATCGAc                            >  2:761913/1‑149 (MQ=255)
                                                                                                                         tAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACt                           >  1:1047968/1‑149 (MQ=255)
                                                                                                                         tAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACt                           >  1:1506187/1‑149 (MQ=255)
                                                                                                                           gAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACttt                         >  2:357872/1‑149 (MQ=255)
                                                                                                                           gAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACttt                         >  1:1098843/1‑149 (MQ=255)
                                                                                                                            aaaaaTACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTg                        >  2:1308429/1‑149 (MQ=255)
                                                                                                                               aaTACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCa                     >  2:1267144/1‑149 (MQ=255)
                                                                                                                                    ccTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAag                >  1:462051/1‑149 (MQ=255)
                                                                                                                                       atcaataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTg                        >  2:445511/7‑139 (MQ=255)
                                                                                                                                       atcaataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTg                        <  1:445511/133‑1 (MQ=255)
                                                                                                                                         caataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGAATTTGCCATAAagaagc            >  1:552562/5‑149 (MQ=255)
                                                                                                                                         aaaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAagcagc            <  2:403777/149‑1 (MQ=255)
                                                                                                                                           ataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGa          >  2:1149149/3‑149 (MQ=255)
                                                                                                                                           aaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGa          <  1:1235267/149‑1 (MQ=255)
                                                                                                                                           aaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGa          <  1:1177248/149‑1 (MQ=255)
                                                                                                                                           aaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGa          <  2:1240121/149‑1 (MQ=255)
                                                                                                                                           aaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCAAAACCATCGACTTTGCCATAAAGCAGCGa          <  1:124873/149‑1 (MQ=255)
                                                                                                                                              ccGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTc       >  2:1324997/1‑149 (MQ=255)
                                                                                                                                               cGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGC‑‑TTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAAt    <  2:68901/149‑1 (MQ=255)
                                                                                                                                                gCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCaa     <  1:1361228/149‑1 (MQ=255)
                                                                                                                                                  aaTTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAAtt   <  1:173581/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTA  >  NC_000913/1821093‑1821387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: