Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,821,244 A→T 5.8% intergenic (‑30/+55) chbC ← / ← chbB N,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS/N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,821,2440AT 5.8% 464.2 / 13.0 155intergenic (‑30/+55)chbC/chbBN,N'‑diacetylchitobiose‑specific enzyme IIC component of PTS/N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base A (74/72);  new base T (6/3);  total (80/75)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

Only 100 of 159 total aligned reads displayed.

GGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACTTC  >  NC_000913/1821097‑1821391
                                                                                                                                                    |                                                                                                                                                   
gggTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTaaa                                                                                                                                                    <  1:2372/149‑1 (MQ=255)
  gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAata                                                                                                                                                  >  1:102605/1‑149 (MQ=255)
  gTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAata                                                                                                                                                  >  2:828023/1‑149 (MQ=255)
     aaCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTg                                                                                                                                               <  1:575748/149‑1 (MQ=255)
      aCGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGAACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGc                                                                                                                                              >  2:388933/1‑149 (MQ=255)
       cGGCATTAAGCGAATAAAGCCATTTTTGATTGCAATAACGTGTGGCTGCTTTCCTATTTTAACTTCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCg                                                                                                                                             <  2:148135/149‑1 (MQ=255)
       cGGCATTAAGCGAATAAAGCCATTTTTGATCGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCg                                                                                                                                             <  1:1195079/149‑1 (MQ=255)
         gCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTAAAATATTGCGGt                                                                                                                                           >  2:745738/1‑149 (MQ=255)
         gCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATATTTAAATTGCGGt                                                                                                                                           <  2:1058579/149‑1 (MQ=255)
            ttAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTAtt                                                                                                                                        >  2:1257880/1‑149 (MQ=255)
              aaGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAACGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGa                                                                                                                                      <  2:157942/149‑1 (MQ=255)
               aGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGAt                                                                                                                                     >  2:1248191/1‑149 (MQ=255)
               aGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGAt                                                                                                                                     <  2:505201/149‑1 (MQ=255)
                 cGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATATTTAAATTGCGGtttttaagg                                                                                                                                   <  1:809463/149‑6 (MQ=255)
                   aaTAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTAt                                                                                                                                 <  2:614346/149‑1 (MQ=255)
                    aTAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGAAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGAGGTATTGATTTATg                                                                                                                                >  1:1045024/1‑149 (MQ=255)
                       aaGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGaa                                                                                                                              >  2:768081/1‑149 (MQ=255)
                       aaGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGaaa                                                                                                                             <  2:102605/149‑1 (MQ=255)
                        aGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAAt                                                                                                                            >  1:858467/1‑149 (MQ=255)
                         gCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATa                                                                                                                           >  2:545380/1‑149 (MQ=255)
                         gCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATa                                                                                                                           >  2:2442/1‑149 (MQ=255)
                              ttttGATTGCATTAACTTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGATGAGTACATTTTCTAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAActct                                                                                                                      <  1:306561/149‑1 (MQ=255)
                                ttGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCttt                                                                                                                    <  2:582925/149‑1 (MQ=255)
                                  gATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGa                                                                                                                  <  1:1223679/149‑1 (MQ=255)
                                   aTTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGAc                                                                                                                 >  1:504457/1‑149 (MQ=255)
                                    ttGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACg                                                                                                                <  1:496697/149‑1 (MQ=255)
                                     tGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACgg                                                                                                               >  1:46139/1‑149 (MQ=255)
                                       cATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGa                                                                                                             >  1:468223/1‑149 (MQ=255)
                                        aTTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGaa                                                                                                            >  1:981918/1‑149 (MQ=255)
                                            aCGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAAtt                                                                                                        >  2:68172/1‑149 (MQ=255)
                                            aCGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAAtt                                                                                                        >  2:9308/1‑149 (MQ=255)
                                            aCGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTATCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAAtt                                                                                                        <  1:277337/149‑1 (MQ=255)
                                                 tgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTaaaa                                                                                                   <  1:545380/149‑1 (MQ=255)
                                                 tgGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACACTTAAAAACCGCAATTTAAATATTGCGGTATCGATTTATGAAATAACTCTTTGACGGGAAAATTTaaaa                                                                                                   >  1:1122249/1‑149 (MQ=255)
                                                  ggCTGCTTTCCTATTTTAACTGTAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaat                                                                                                  >  1:403777/1‑149 (MQ=255)
                                                   gctgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaata                                                                                                 >  2:565736/1‑149 (MQ=255)
                                                    ctgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataa                                                                                                >  1:1022422/1‑149 (MQ=255)
                                                    ctgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataa                                                                                                <  1:1426680/149‑1 (MQ=255)
                                                     tgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaa                                                                                               <  2:1101870/149‑1 (MQ=255)
                                                     tgctTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaa                                                                                               <  2:127049/149‑1 (MQ=255)
                                                     tgctTTACTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAaaataaa                                                                                               <  1:1084807/149‑1 (MQ=255)
                                                        tttCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaatta                                                                                            >  1:1240121/1‑149 (MQ=255)
                                                          tCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAaattaat                                                                                          <  2:630248/149‑1 (MQ=255)
                                                             tATTTTAACTTCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtg                                                                                       <  1:1600258/149‑1 (MQ=255)
                                                               ttttAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTtgct                                                                                     <  1:68172/149‑1 (MQ=255)
                                                                   aaCTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCgg                                                                                 <  2:292757/149‑1 (MQ=255)
                                                                      tGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtt                                                                              <  1:69050/149‑1 (MQ=255)
                                                                        cAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttt                                                                            <  2:1072110/149‑1 (MQ=255)
                                                                        cAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCtttt                                                                            <  2:1524108/149‑1 (MQ=255)
                                                                         aaaaGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCttttt                                                                           >  1:1092694/1‑149 (MQ=255)
                                                                             gggAGGATTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAAt                                                                       <  1:909826/149‑1 (MQ=255)
                                                                             gggAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTaa                                                                        <  1:768081/149‑1 (MQ=255)
                                                                               taggagTACCTTTTCAATAGATGCAATAACATTACTCATAGAAAAATACACTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACTGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCg                                                                     <  1:43639/148‑1 (MQ=255)
                                                                                aggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGc                                                                    >  2:717396/1‑149 (MQ=255)
                                                                                 ggagTACCTTTTCAAGCGATGCAATAACATTACTCTTAGAAAAATACCCTTAAAAACCGCATTTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCt                                                                   <  2:1424542/149‑1 (MQ=255)
                                                                                 ggagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCt                                                                   <  1:1135134/149‑1 (MQ=255)
                                                                                  gagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTg                                                                  <  2:313241/149‑1 (MQ=255)
                                                                                  gagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTg                                                                  >  2:927007/1‑149 (MQ=255)
                                                                                  gagTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTg                                                                  >  2:97823/1‑149 (MQ=255)
                                                                                    gTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTaa                                                                        >  1:64479/1‑141 (MQ=255)
                                                                                    gTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTaa                                                                        <  2:64479/141‑1 (MQ=255)
                                                                                     tACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                               >  2:475143/1‑149 (MQ=255)
                                                                                     tACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                               >  2:1316432/1‑149 (MQ=255)
                                                                                     tACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                               >  2:1577747/1‑149 (MQ=255)
                                                                                      aCCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAAc                                                              <  2:1085400/149‑1 (MQ=255)
                                                                                        cTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAAATTAAATATTGCGGTATTGATTTATGAAATAACTATTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGTCTTTTTTAATCGCTGCAACCg                                                            >  1:309756/1‑149 (MQ=255)
                                                                                              aaGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTTCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGcat                                                      >  2:1488805/1‑147 (MQ=255)
                                                                                                 cGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTaa                                                   >  1:77748/1‑149 (MQ=255)
                                                                                                  gATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAg                                                  <  2:507066/149‑1 (MQ=255)
                                                                                                  gATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAg                                                  <  1:828023/149‑1 (MQ=255)
                                                                                                     gCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCAc                                               <  1:401520/149‑1 (MQ=255)
                                                                                                       aaTAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGc                                             >  1:674389/1‑149 (MQ=255)
                                                                                                            cATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTaaa                                        <  1:1257880/149‑1 (MQ=255)
                                                                                                            aaTTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTaaa                                        <  1:1173428/148‑1 (MQ=255)
                                                                                                             aTTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAAc                                       <  2:338467/149‑1 (MQ=255)
                                                                                                              ttACTCATAGATAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAAcc                                      >  2:404811/1‑149 (MQ=255)
                                                                                                                aCTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCAt                                    <  1:647041/149‑1 (MQ=255)
                                                                                                                   cATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGa                                 >  2:837087/1‑149 (MQ=255)
                                                                                                                    aTAGAAAGATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGAc                                >  2:1363328/1‑149 (MQ=255)
                                                                                                                    aTAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                               >  2:394202/1‑118 (MQ=255)
                                                                                                                    aTAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCaa                                                               <  1:394202/118‑1 (MQ=255)
                                                                                                                    aTAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTATGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTTAACCATCGAc                                >  2:761913/1‑149 (MQ=255)
                                                                                                                     tAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACt                               >  1:1047968/1‑149 (MQ=255)
                                                                                                                     tAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACt                               >  1:1506187/1‑149 (MQ=255)
                                                                                                                     tAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACt                               >  2:1164049/1‑149 (MQ=255)
                                                                                                                            aTACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCAt                        <  2:1045024/149‑1 (MQ=255)
                                                                                                                                   cttaaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAagca                  >  1:549486/4‑149 (MQ=255)
                                                                                                                                   atcaataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTg                            <  1:445511/133‑1 (MQ=255)
                                                                                                                                      aaaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCg               >  2:186915/1‑149 (MQ=255)
                                                                                                                                      aaaaACGCAATATAAATATTGCGGTATTGATTTATGAAATAACTCTATGACGGGAAAATTTAAAATAAATTAATTTGCTTCGTCTTTTTTAATCTATGCAACCGCAGCCTTAAGCACGCATAAACCATCGACTTTTACATAAagaagct               >  1:1349396/1‑148 (MQ=255)
                                                                                                                                       ataCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGa              >  2:1149149/3‑149 (MQ=255)
                                                                                                                                       aaaCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGa              <  2:1240121/149‑1 (MQ=255)
                                                                                                                                        taCCGCAATATTTAAATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATAAATTAGCTGCGGCTTTTATAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTAGCCATAAAGCAGCGat             >  2:1270443/2‑148 (MQ=255)
                                                                                                                                        taCCGCAATATTTAAATTGCGGTATTGATTCATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAg             >  2:1211155/2‑149 (MQ=255)
                                                                                                                                         aCCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGt            <  2:46139/149‑1 (MQ=255)
                                                                                                                                           cGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGC‑‑TTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAAt        <  2:68901/149‑1 (MQ=255)
                                                                                                                                             cAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAAt        <  2:1264485/149‑1 (MQ=255)
                                                                                                                                                  tAAATATTTCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATATGCTGCGTCTTTTTTAATCTCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACtt   <  1:456314/149‑1 (MQ=255)
                                                                                                                                                  tAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACtt   <  2:1128520/149‑1 (MQ=255)
                                                                                                                                                   aaaTATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACTTc  <  2:779332/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAAGGGAGGAGTACCTTTTCAAGCGATGCAATAACATTACTCATAGAAAAATACCCTTAAAAACCGCAATTTAAATATTGCGGTATTGATTTATGAAATAACTCTTTGACGGGAAAATTTAAAATAAATTAATTTGCTGCGGCTTTTTTAATCGCTGCAACCGCAGCCTTAAGCACGCCTAAACCATCGACTTTGCCATAAAGCAGCGAGTCAATTACTTC  >  NC_000913/1821097‑1821391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: