Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,870,253 T→C 7.6% intergenic (+15/‑132) yeaH → / → yeaI UPF0229 family protein/putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,870,2530TC 7.6% 423.3 / 18.9 145intergenic (+15/‑132)yeaH/yeaIUPF0229 family protein/putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (75/57);  new base C (9/2);  total (85/60)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 145 total aligned reads displayed.

ATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACAC  >  NC_000913/1870106‑1870400
                                                                                                                                                   |                                                                                                                                                     
aTTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTc                                                                                                                                                      >  1:766395/1‑149 (MQ=255)
aTTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTACGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTc                                                                                                                                                      >  2:1069282/1‑149 (MQ=255)
    tgGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGATCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTa                                                                                                                                                  >  1:210273/1‑149 (MQ=255)
      gCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATc                                                                                                                                                >  1:1051065/1‑149 (MQ=255)
        gagaATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGc                                                                                                                                              >  1:816164/1‑149 (MQ=255)
          gaATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCt                                                                                                                                            <  2:1522352/149‑1 (MQ=255)
          gaATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCt                                                                                                                                            >  2:170352/1‑149 (MQ=255)
           aaTATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTg                                                                                                                                           <  1:485900/149‑1 (MQ=255)
             tatGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGCGATAATGACCTGGc                                                                                                                                         <  2:173885/149‑1 (MQ=255)
               tGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCATGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTg                                                                                                                                       >  1:1149178/1‑149 (MQ=255)
                  gCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAt‑‑t                                                                                                                                  >  2:574276/1‑149 (MQ=255)
                    aTCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAt‑‑ttt                                                                                                                                <  1:359835/149‑1 (MQ=255)
                    aTCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAt‑‑ttt                                                                                                                                <  2:658654/149‑1 (MQ=255)
                         cAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCt                                                                                                                           >  2:959353/1‑149 (MQ=255)
                           aTCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTa                                                                                                                         >  1:1099501/1‑149 (MQ=255)
                           aTCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACACCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTa                                                                                                                         <  1:847430/149‑1 (MQ=255)
                                cTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTa                                                                                                                    <  2:522183/149‑1 (MQ=255)
                                 tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTaa                                                                                                                   >  1:908030/1‑149 (MQ=255)
                                 tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTaa                                                                                                                   >  1:1495907/1‑149 (MQ=255)
                                 tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTaa                                                                                                                   >  1:1207514/1‑149 (MQ=255)
                                   tCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAAt                                                                                                                 <  2:210273/149‑1 (MQ=255)
                                     gACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTa                                                                                                               >  2:204109/1‑149 (MQ=255)
                                       cAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTAtc                                                                                                             <  1:204109/149‑1 (MQ=255)
                                        aaCTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTAtct                                                                                                            >  2:685289/1‑149 (MQ=255)
                                          cTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCa                                                                                                          >  1:406957/1‑149 (MQ=255)
                                           tttGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCAt                                                                                                         >  1:12933/1‑149 (MQ=255)
                                           tttGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCAt                                                                                                         >  1:1349054/1‑149 (MQ=255)
                                               cGATTCAGCACATCCGCTACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttat                                                                                                     <  2:248138/149‑1 (MQ=255)
                                                gATGCAGCACATCCGCTACCATGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                    <  1:584328/149‑1 (MQ=255)
                                                gATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                    <  1:301412/149‑1 (MQ=255)
                                                gATGCAGCACATCCGCGACAAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACAATCAGCCAGGTCATTTTCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                    >  2:1264017/1‑149 (MQ=255)
                                                      gcaCATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtacatac                                                                                              <  2:701301/149‑1 (MQ=255)
                                                       cacaTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTTTCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtacataca                                                                                             >  2:163053/1‑149 (MQ=255)
                                                         caTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACAtt                                                                                           <  2:1397079/149‑1 (MQ=255)
                                                          aTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCGAGGTCATTATCGCCTGGATGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTa                                                                                          >  1:26829/1‑149 (MQ=255)
                                                          aTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGATAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGATTACTGTAAATTATCTCATTTATTacatacatga                                                                                          >  1:26832/1‑147 (MQ=255)
                                                           tCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAg                                                                                         <  1:1044792/149‑1 (MQ=255)
                                                             cgcgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCt                                                                                       <  1:1490745/149‑1 (MQ=255)
                                                               cgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTa                                                                                     <  1:1590179/149‑1 (MQ=255)
                                                               cgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTa                                                                                     <  2:1213953/149‑1 (MQ=255)
                                                                 aCCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTAAATACATTAGCTTACa                                                                                   >  1:566165/1‑149 (MQ=255)
                                                                      gatgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCga                                                                              >  2:351583/1‑148 (MQ=255)
                                                                      gatgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGc                                                                              <  2:621399/149‑1 (MQ=255)
                                                                      gatgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGc                                                                              >  1:94717/1‑149 (MQ=255)
                                                                        tgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCtt                                                                            >  1:1253895/1‑149 (MQ=255)
                                                                           tatTTATCCGGTGTTCCGTGAACTGTTTAATATACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTAATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaa                                                                         <  1:764935/149‑1 (MQ=255)
                                                                            atTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaaa                                                                        <  2:1388693/149‑1 (MQ=255)
                                                                            atTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaaa                                                                        <  2:332563/149‑1 (MQ=255)
                                                                             tttATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGTCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAat                                                                       <  2:631568/149‑1 (MQ=255)
                                                                               tATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAatat                                                                     <  2:835459/149‑1 (MQ=255)
                                                                                 tCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGa                                                                   >  1:1432798/1‑149 (MQ=255)
                                                                                 tCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGa                                                                   <  1:1488876/149‑1 (MQ=255)
                                                                                  ccGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGAc                                                                  <  2:512956/149‑1 (MQ=255)
                                                                                        ttCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATa                                                            >  1:491810/1‑149 (MQ=255)
                                                                                           cGTTAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGTCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATTACAGCATAAcc                                                         <  1:1594321/149‑1 (MQ=255)
                                                                                               aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                     >  2:1298993/1‑149 (MQ=255)
                                                                                               aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                     <  2:1099501/149‑1 (MQ=255)
                                                                                               aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                     <  1:1229275/149‑1 (MQ=255)
                                                                                                    tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                >  2:1582503/1‑149 (MQ=255)
                                                                                                    tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                >  1:1050806/1‑149 (MQ=255)
                                                                                                    tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                >  2:1498517/1‑149 (MQ=255)
                                                                                                        aTAAACAAAATGCAACAGCTAAAGGCTAAAACTATTAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTAAAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTa                                            >  1:1279933/1‑149 (MQ=255)
                                                                                                        aTAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCACATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATACCCTTTACATAATTTa                                            >  1:116440/1‑149 (MQ=255)
                                                                                                         tAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAg                                           <  1:170352/149‑1 (MQ=255)
                                                                                                          aaaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGt                                          >  1:1376965/1‑149 (MQ=255)
                                                                                                           aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGGCAGCATAACCTTTACATAATTTAGtt                                         >  2:787703/1‑149 (MQ=255)
                                                                                                           aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt                                         >  2:1579797/1‑149 (MQ=255)
                                                                                                           aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt                                         >  2:898274/1‑149 (MQ=255)
                                                                                                                   gCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGaaaa                                 <  1:567118/149‑1 (MQ=255)
                                                                                                                      aCAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACaa                              <  2:419073/149‑1 (MQ=255)
                                                                                                                        aGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATc                            >  2:729709/1‑149 (MQ=255)
                                                                                                                          cTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAt                          >  1:636865/1‑149 (MQ=255)
                                                                                                                           tAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCAAGAAAACAATCAtt                         >  1:442413/1‑149 (MQ=255)
                                                                                                                            aaaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTc                        <  1:685289/149‑1 (MQ=255)
                                                                                                                            aaaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTc                        <  1:574276/149‑1 (MQ=255)
                                                                                                                             aatgCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg                       <  2:967177/146‑1 (MQ=255)
                                                                                                                             aaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg                       >  2:404165/1‑149 (MQ=255)
                                                                                                                             aaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACATTTACATAATTTAGTTCCAGAAAACAATCATTCg                       >  1:987265/1‑149 (MQ=255)
                                                                                                                               ggCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGa                     >  2:1261450/1‑149 (MQ=255)
                                                                                                                                gCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaa                    >  1:1518833/1‑149 (MQ=255)
                                                                                                                                 cTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaa                   >  2:1153639/1‑149 (MQ=255)
                                                                                                                                   ctaaaaaTCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaaa                 >  1:1126701/7‑149 (MQ=39)
                                                                                                                                    taaaaaTCAGCCAGGCGATAATGACCTGGCTGATAGTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaa                  >  1:936654/6‑149 (MQ=37)
                                                                                                                                    aaaCTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAAt                >  2:1293118/1‑149 (MQ=255)
                                                                                                                                    aaaCTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAAt                >  2:1045142/1‑149 (MQ=255)
                                                                                                                                     aaaaaTCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATAGCTTTAAATATTACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATAATTCGGAAAAATg               >  2:1030066/5‑149 (MQ=255)
                                                                                                                                        tATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt            <  2:1555455/149‑1 (MQ=255)
                                                                                                                                        tATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTATACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt            >  2:848308/1‑149 (MQ=255)
                                                                                                                                        tATCAGCCAGGTAATTATCGCCTGGCTGAT‑‑TTTTAGCTTAATGTAAATTATATCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGTAAAATGAtt            <  2:260880/149‑1 (MQ=255)
                                                                                                                                          ttaGCCAGGCGATAATGACCTGGCTGATAGTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt            <  2:896339/147‑1 (MQ=38)
                                                                                                                                          tCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa          >  1:1343909/1‑149 (MQ=255)
                                                                                                                                          tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa          >  2:1120205/1‑149 (MQ=255)
                                                                                                                                          tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa          >  1:994537/1‑149 (MQ=255)
                                                                                                                                          tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa          >  1:869564/1‑149 (MQ=255)
                                                                                                                                          tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa          >  1:630228/1‑149 (MQ=255)
                                                                                                                                           cAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAg         <  2:12933/149‑1 (MQ=255)
                                                                                                                                            aGCCAGGACATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGt        >  1:406840/1‑149 (MQ=255)
                                                                                                                                             gCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc       >  2:547960/1‑149 (MQ=255)
                                                                                                                                              ccAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCa      >  2:429776/1‑149 (MQ=255)
                                                                                                                                                aGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAac    <  2:1582659/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACAC  >  NC_000913/1870106‑1870400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: