Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 314,578 G→A 6.8% pseudogene (222/872 nt) eaeH → pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913314,5780GA 6.8% 281.9 / 6.8 92pseudogene (222/872 nt)eaeHpseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base G (33/51);  new base A (3/3);  total (37/55)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.64e-01

TATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACT  >  NC_000913/314434‑314721
                                                                                                                                                |                                                                                                                                               
tatCTCTGTTCAGGGTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTg                                                                                                                                             <  2:233081/149‑1 (MQ=255)
  tctcTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGcc                                                                                                                                           <  2:1342435/149‑1 (MQ=255)
   ctctGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCg                                                                                                                                          >  2:653597/1‑149 (MQ=255)
   ctctGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCg                                                                                                                                          <  2:843660/149‑1 (MQ=255)
    tctGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGc                                                                                                                                         >  1:1383739/1‑149 (MQ=255)
      tGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACAGCTGATAATAACTTGGAGAAAAAAGTCTCGTCCTTTGCCGCaa                                                                                                                                       >  2:1517373/1‑149 (MQ=255)
       gTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCaaa                                                                                                                                      <  1:159964/149‑1 (MQ=255)
         tCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCaaagg                                                                                                                                    >  1:1535897/1‑147 (MQ=255)
           aGGTTCTTTTTCCACTCGCTGTCCCCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCAGGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGcc                                                                                                                                  >  2:1474158/1‑149 (MQ=255)
            ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGTACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  2:418972/149‑1 (MQ=255)
            ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  2:492040/149‑1 (MQ=255)
            ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  1:1266803/149‑1 (MQ=255)
            ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  1:872171/149‑1 (MQ=255)
            ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  1:25567/149‑1 (MQ=255)
            ggTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCg                                                                                                                                 <  1:1388257/149‑1 (MQ=255)
             gTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACTGTAACTGCTGATAATAACGTGGAGAAAAATGACGCGTCGTTTGCCGCAAATGCCgg                                                                                                                                >  2:978269/1‑149 (MQ=255)
              ttCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATTACGTGGAGATAAATGTCGCGTCGTTTGCCGCTTATGCCggg                                                                                                                               >  1:629261/1‑149 (MQ=255)
              ttCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCggg                                                                                                                               >  2:1586033/1‑149 (MQ=255)
               tCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGa                                                                                                                              <  2:1528702/149‑1 (MQ=255)
               tCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGa                                                                                                                              <  1:598328/149‑1 (MQ=255)
                cTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACTTCGAGAAAAATGTCGCGTCGTATGCCGCAAATGCCGGGAc                                                                                                                             >  2:248610/1‑149 (MQ=255)
                   tttCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTGCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTGTGCCGCAAATGCCGGGACAtt                                                                                                                          <  2:641643/149‑1 (MQ=255)
                    ttCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACAttt                                                                                                                         <  2:598084/149‑1 (MQ=255)
                      ccACTCGCTGTCCCCTTTACCCCAGTAATGGCGGCACTTGCGCAGCATGCGGGTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAttttt                                                                                                                       <  2:1149062/149‑1 (MQ=37)
                      ccACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAttttt                                                                                                                       <  2:1494512/149‑1 (MQ=38)
                       cACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAtttttc                                                                                                                      <  2:527536/149‑2 (MQ=38)
                        aCTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTaa                                                                                                                     <  2:1444087/149‑1 (MQ=255)
                         cTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                    <  2:1535897/149‑1 (MQ=255)
                         cTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                    >  2:1227641/1‑149 (MQ=255)
                         cTCGCTGGCACCTTTACCCCAGAAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                    <  1:365269/149‑1 (MQ=255)
                           cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCa                                                                                                                  <  1:1517673/149‑1 (MQ=255)
                           cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCa                                                                                                                  <  2:653514/149‑1 (MQ=255)
                           cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCa                                                                                                                  <  1:670290/149‑1 (MQ=255)
                                  aCCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGcc                                                                                                           >  2:1282721/1‑149 (MQ=255)
                                  aCCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGcc                                                                                                           <  2:681544/149‑1 (MQ=255)
                                   ccTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGCGCAGCCACGGGTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCa                                                                                                          <  1:930139/149‑1 (MQ=255)
                                       tACCCCAGTAATGGCGGCACGGGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATa                                                                                                      <  2:169092/149‑1 (MQ=255)
                                          cccAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCg                                                                                                   >  1:1463218/1‑149 (MQ=255)
                                          cccAGTAATGGCGGCACGTGCGCAGCATGCGGTTAAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCg                                                                                                   >  2:958529/1‑149 (MQ=255)
                                            cAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                 <  1:667921/149‑1 (MQ=255)
                                            cAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                 >  1:1472424/1‑149 (MQ=255)
                                            cAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                 <  1:1224959/149‑1 (MQ=255)
                                             aGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATg                                                                                                <  1:972749/149‑1 (MQ=255)
                                              gTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGc                                                                                               <  1:1257966/149‑1 (MQ=255)
                                                aaTGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGa                                                                                             <  2:665147/149‑1 (MQ=255)
                                                       gcACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCCTGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTaa                                                                                      <  1:1267052/149‑1 (MQ=255)
                                                          agTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGTGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACtt                                                                                   <  2:868142/148‑1 (MQ=255)
                                                            tGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATTACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGGCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                 <  2:229981/149‑1 (MQ=255)
                                                            tGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTACTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                 >  1:1273781/1‑149 (MQ=255)
                                                                 agcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTAcc                                                                            >  2:815561/1‑149 (MQ=255)
                                                                 agcaTGCGGCTCAGCCACGGTGGAGCATGGGAAATACTACTGTAACTGCTGATAATAACGTGGAGAAATCTGTCGCGTCGTTTGCCGCAAATGCCGGGACATGTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTAcc                                                                            <  1:982015/149‑1 (MQ=255)
                                                                  gcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCg                                                                           <  1:1297517/149‑1 (MQ=255)
                                                                  gcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCg                                                                           >  2:106277/1‑149 (MQ=255)
                                                                        ggTGCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATgg                                                                     <  2:569508/149‑1 (MQ=255)
                                                                         gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGc                                                                    >  1:1230415/1‑149 (MQ=255)
                                                                         gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGc                                                                    >  2:795635/1‑149 (MQ=255)
                                                                         gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGc                                                                    >  2:400137/1‑149 (MQ=255)
                                                                           tctgCCACGGTTGAGCATTGGAAATAATACTGTAAATGCTTATTATAACGTGGAGAAAAATGTAGAGTCGGTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCca                                                                  <  2:1430818/146‑1 (MQ=255)
                                                                                    gTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACagctaaag                                                         <  2:187128/149‑1 (MQ=255)
                                                                                         gCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAAc                                                    <  2:1382062/149‑1 (MQ=255)
                                                                                          cATGGGAATTACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCAACAGCTAAAGCTAAcc                                                   >  2:1451210/1‑149 (MQ=255)
                                                                                           aTGGGAAATACTACGGTAACTGCTGATAATTACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTTCCGGAATGGCCACAGCTAAAGCTAACCa                                                  <  2:1570621/149‑1 (MQ=255)
                                                                                           aTGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCa                                                  <  2:923534/149‑1 (MQ=255)
                                                                                            tGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAg                                                 <  1:1576907/149‑1 (MQ=255)
                                                                                                aaaTACTACGGTAACTGATGATAATAACGTCGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACATCTAAAGCTAACCAGGaaa                                             <  1:1517373/149‑1 (MQ=255)
                                                                                                   tactacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATAc                                          >  1:1496565/1‑149 (MQ=255)
                                                                                                    actacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCCTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACa                                         <  2:322192/149‑1 (MQ=255)
                                                                                                     ctacTGTAACTGCTGATAATAAAGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAg                                        <  2:491561/149‑1 (MQ=255)
                                                                                                      tacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                 <  1:1052437/107‑1 (MQ=255)
                                                                                                      tacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                 >  2:1052437/1‑107 (MQ=255)
                                                                                                       acGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGa                                      <  1:653597/149‑1 (MQ=255)
                                                                                                          gTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTg                                   >  2:1343454/1‑149 (MQ=255)
                                                                                                            aaCTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGAGATGAGACACGTAACTGTATTACCGGAATGGCCACAGCTAAAGCTATCGAGGAATTACAGGAGTgga                                 >  2:207436/1‑148 (MQ=255)
                                                                                                              ctgctgATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTc                               <  2:1545100/149‑1 (MQ=255)
                                                                                                               tgctgATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCg                              >  1:605513/1‑149 (MQ=255)
                                                                                                                    ataataACGTGGAGAAAAATGTCGCGTCGTTTGACGCAAATGACGGGACATTTTTAAGAAGACAGCCAGATAGCGATGCGACTCGTAACTTTATTACCTGAATGTAAACAGCTAAATCTAAACAGTAAATACATTAGTGGATCGGTaaa                         >  1:988973/1‑149 (MQ=255)
                                                                                                                      aataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGAGATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTAATTACCGGAATGGCCACAGCTAAAGCTAACAAGGAAATACAGGAGAGGCTCGGGAAata                       >  2:609302/1‑149 (MQ=255)
                                                                                                                      aataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAata                       <  1:178404/149‑1 (MQ=255)
                                                                                                                       ataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAatat                      >  1:1051050/1‑149 (MQ=255)
                                                                                                                        taaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATg                     <  1:10704/149‑1 (MQ=255)
                                                                                                                           cGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTTGGGAAATATGGTa                  <  1:106277/149‑1 (MQ=255)
                                                                                                                           cGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTa                  <  2:1155907/149‑1 (MQ=255)
                                                                                                                            gTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTAc                 >  2:609088/1‑149 (MQ=255)
                                                                                                                             tgcttaaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACt                >  1:19371/6‑149 (MQ=37)
                                                                                                                                ttaaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcg             >  2:484492/3‑149 (MQ=38)
                                                                                                                                  aaaaaTGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAAGTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCACGGGAAATATGGTACTgcgcg           >  2:875986/1‑149 (MQ=255)
                                                                                                                                  aaaaaTGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcgcg           >  1:553622/1‑149 (MQ=255)
                                                                                                                                  aaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcgcg           >  2:721573/1‑149 (MQ=38)
                                                                                                                                        gTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCaa     >  2:1376189/1‑149 (MQ=255)
                                                                                                                                         tCGCGTCGTTTGCCGCAAATGCCGGTACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCaaa    <  1:978269/149‑1 (MQ=255)
                                                                                                                                         tCGCGTCGTTTGCAGCAAATTCCGGGACATTATTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCaaa    <  2:817048/149‑1 (MQ=255)
                                                                                                                                           gcgTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACt  >  1:594956/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACT  >  NC_000913/314434‑314721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑