Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,870,257 T→A 7.4% intergenic (+19/‑128) yeaH → / → yeaI UPF0229 family protein/putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,870,2570TA 7.4% 414.3 / 17.3 145intergenic (+19/‑128)yeaH/yeaIUPF0229 family protein/putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (76/58);  new base A (9/2);  total (85/60)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.18e-01

Only 100 of 148 total aligned reads displayed.

TGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAT  >  NC_000913/1870110‑1870404
                                                                                                                                                   |                                                                                                                                                     
tgGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGATCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTa                                                                                                                                                      >  1:210273/1‑149 (MQ=255)
tgGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTa                                                                                                                                                      <  2:1235996/149‑1 (MQ=255)
  gCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAATCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATc                                                                                                                                                    >  1:482555/1‑149 (MQ=255)
  gCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATc                                                                                                                                                    >  1:1051065/1‑149 (MQ=255)
   cGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCg                                                                                                                                                   <  1:201107/149‑1 (MQ=255)
         tatGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGCGATAATGACCTGGc                                                                                                                                             <  2:173885/149‑1 (MQ=255)
         tatGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCAGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGACTAAAACTATCAGCCAGGTCATTATCGCCTggg                                                                                                                                             >  1:248138/1‑148 (MQ=255)
           tGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCATGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTg                                                                                                                                           >  1:1149178/1‑149 (MQ=255)
              gCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAt‑‑t                                                                                                                                      >  2:574276/1‑149 (MQ=255)
                aTCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAt‑‑ttt                                                                                                                                    <  2:658654/149‑1 (MQ=255)
                     cAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCt                                                                                                                               >  2:959353/1‑149 (MQ=255)
                     cAATCTACTTTCGACAAATTAGCGATGCACGACATCCGCGACCAGTATGATATTTATCCGGTGTTCCGTGAACTGATTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGCTCATTATCGCCTGGCTGAT‑‑TTTTAGCt                                                                                                                               <  2:1582203/149‑1 (MQ=255)
                       aTCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTa                                                                                                                             >  1:1099501/1‑149 (MQ=255)
                       aTCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTa                                                                                                                             >  1:593740/1‑149 (MQ=255)
                       aTCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACACCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTa                                                                                                                             <  1:847430/149‑1 (MQ=255)
                        tCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTAc                                                                                                                            >  1:1364059/1‑149 (MQ=255)
                         cTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGTATGATATTTATCCGGGGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACt                                                                                                                           <  1:1495862/149‑1 (MQ=255)
                            cTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTa                                                                                                                        <  2:522183/149‑1 (MQ=255)
                             tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCTCCTGGCTTAT‑‑TTTTAGCTTACAGTaa                                                                                                                       >  2:127961/1‑149 (MQ=255)
                             tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTaa                                                                                                                       >  1:1207514/1‑149 (MQ=255)
                             tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTaa                                                                                                                       >  1:1495907/1‑149 (MQ=255)
                             tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTaa                                                                                                                       >  1:908030/1‑149 (MQ=255)
                               tCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAAt                                                                                                                     <  2:210273/149‑1 (MQ=255)
                                      cTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCa                                                                                                              >  1:1582659/1‑149 (MQ=255)
                                            gATGCAGCACATCCGCTACCATGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                        <  1:584328/149‑1 (MQ=255)
                                            gATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                        <  1:301412/149‑1 (MQ=255)
                                            gATGCAGCACATCCGCGACAAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACAATCAGCCAGGTCATTTTCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                        >  2:1264017/1‑149 (MQ=255)
                                             aTGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatta                                                                                                       <  2:1349054/149‑1 (MQ=255)
                                              tGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtac                                                                                                      <  1:572692/149‑1 (MQ=255)
                                                cagcaCATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtacat                                                                                                    >  2:1412969/1‑149 (MQ=255)
                                                  gcaCATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtacatac                                                                                                  <  2:701301/149‑1 (MQ=255)
                                                   cacaTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTTTCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtacataca                                                                                                 >  2:163053/1‑149 (MQ=255)
                                                      aTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTa                                                                                              >  1:51389/1‑149 (MQ=255)
                                                      aTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGATAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGATTACTGTAAATTATCTCATTTATTacatacatga                                                                                              >  1:26832/1‑147 (MQ=255)
                                                       tCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAg                                                                                             <  1:1044792/149‑1 (MQ=255)
                                                         cgcgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCt                                                                                           >  2:166898/1‑149 (MQ=255)
                                                           cgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTa                                                                                         <  1:1590179/149‑1 (MQ=255)
                                                           cgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTa                                                                                         <  2:1213953/149‑1 (MQ=255)
                                                                  gatgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGc                                                                                  <  2:621399/149‑1 (MQ=255)
                                                                  gatgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGc                                                                                  >  1:94717/1‑149 (MQ=255)
                                                                   atgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCt                                                                                 <  2:27247/149‑1 (MQ=255)
                                                                    tgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCtt                                                                                >  1:1253895/1‑149 (MQ=255)
                                                                       tttttATCTGGTTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaaa                                                                            <  1:649824/147‑1 (MQ=255)
                                                                       tatTTATCCGGTGTTCCGTGAACTGTTTAATATACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTAATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaa                                                                             <  1:764935/149‑1 (MQ=255)
                                                                        atTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaaa                                                                            <  2:1388693/149‑1 (MQ=255)
                                                                        atTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaaa                                                                            <  2:332563/149‑1 (MQ=255)
                                                                           tATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAatat                                                                         <  2:835459/149‑1 (MQ=255)
                                                                             tCCGGTGTTCCGTGATCTGTTTCATATACATAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGGCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATCTATAACATACATTAGCTTACTATCGCTTAAAATATGa                                                                       <  2:195903/149‑1 (MQ=255)
                                                                             tCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGa                                                                       <  1:1488876/149‑1 (MQ=255)
                                                                              ccGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGAc                                                                      <  2:512956/149‑1 (MQ=255)
                                                                               cGGTGTTCCATGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACa                                                                     <  2:1149178/149‑1 (MQ=255)
                                                                                    ttCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATa                                                                >  2:359024/1‑149 (MQ=255)
                                                                                    ttCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATa                                                                >  1:491810/1‑149 (MQ=255)
                                                                                         tGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCtt                                                           >  2:1548010/1‑149 (MQ=255)
                                                                                         tGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCtt                                                           <  1:814825/149‑1 (MQ=255)
                                                                                          gAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCttt                                                          >  1:858494/1‑149 (MQ=255)
                                                                                           aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                         <  1:1229275/149‑1 (MQ=255)
                                                                                           aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                         >  2:1298993/1‑149 (MQ=255)
                                                                                                tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                    >  2:1582503/1‑149 (MQ=255)
                                                                                                tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                    >  2:1498517/1‑149 (MQ=255)
                                                                                                tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                    >  1:1050806/1‑149 (MQ=255)
                                                                                                    aTAAACAAAATGCAACAGCTAAAGGCTAAAACTATTAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTAAAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTa                                                >  1:1279933/1‑149 (MQ=255)
                                                                                                    aTAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCACATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATACCCTTTACATAATTTa                                                >  1:116440/1‑149 (MQ=255)
                                                                                                     tAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAg                                               <  1:170352/149‑1 (MQ=255)
                                                                                                      aaaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGt                                              >  1:1376965/1‑149 (MQ=255)
                                                                                                       aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGGCAGCATAACCTTTACATAATTTAGtt                                             >  2:787703/1‑149 (MQ=255)
                                                                                                       aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt                                             >  2:898274/1‑149 (MQ=255)
                                                                                                               gCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGaaaa                                     <  1:567118/149‑1 (MQ=255)
                                                                                                                    aGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATc                                >  2:729709/1‑149 (MQ=255)
                                                                                                                      cTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAt                              >  2:1098994/1‑149 (MQ=255)
                                                                                                                      cTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAt                              >  1:636865/1‑149 (MQ=255)
                                                                                                                       tAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCAAGAAAACAATCAtt                             >  1:442413/1‑149 (MQ=255)
                                                                                                                        aaaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTc                            >  2:211553/1‑149 (MQ=255)
                                                                                                                         aatgCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg                           <  2:967177/146‑1 (MQ=255)
                                                                                                                         aaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg                           >  2:404165/1‑149 (MQ=255)
                                                                                                                         aaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACATTTACATAATTTAGTTCCAGAAAACAATCATTCg                           >  1:987265/1‑149 (MQ=255)
                                                                                                                            gCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTTCAGACATTAGCTTACAATCGCTTGAAATATGACAGCATCACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaa                        >  2:104066/1‑149 (MQ=255)
                                                                                                                              tAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaa                      >  2:469305/1‑149 (MQ=255)
                                                                                                                               ctaaaaaTCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaaa                     >  1:1126701/7‑149 (MQ=39)
                                                                                                                                taaaaaTCAGCCAGGCGATAATGACCTGGCTGATAGTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaa                      >  1:936654/6‑149 (MQ=37)
                                                                                                                                aaaCTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAAt                    >  2:1045142/1‑149 (MQ=255)
                                                                                                                                    tATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt                <  2:1555455/149‑1 (MQ=255)
                                                                                                                                    tATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTATACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt                >  2:848308/1‑149 (MQ=255)
                                                                                                                                    tATCAGCCAGGTAATTATCGCCTGGCTGAT‑‑TTTTAGCTTAATGTAAATTATATCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGTAAAATGAtt                <  2:260880/149‑1 (MQ=255)
                                                                                                                                     aTCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtta               >  1:1473284/1‑148 (MQ=255)
                                                                                                                                      ttaGCCAGGCGATAATGACCTGGCTGATAGTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt                <  2:896339/147‑1 (MQ=38)
                                                                                                                                      tCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa              >  1:1343909/1‑149 (MQ=255)
                                                                                                                                      tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa              >  1:994537/1‑149 (MQ=255)
                                                                                                                                      tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa              >  1:301196/1‑149 (MQ=255)
                                                                                                                                      tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa              >  2:1120205/1‑149 (MQ=255)
                                                                                                                                       cAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAg             <  2:12933/149‑1 (MQ=255)
                                                                                                                                        aGCCAGGACATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGt            >  1:406840/1‑149 (MQ=255)
                                                                                                                                         gCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc           >  2:547960/1‑149 (MQ=255)
                                                                                                                                         gCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc           <  2:636865/149‑1 (MQ=255)
                                                                                                                                         gCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc           >  1:361244/1‑149 (MQ=255)
                                                                                                                                          ccAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCa          >  2:429776/1‑149 (MQ=255)
                                                                                                                                              gTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAacac      <  1:1298993/149‑1 (MQ=255)
                                                                                                                                                 aTTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTa   >  2:601474/1‑149 (MQ=255)
                                                                                                                                                  ttATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAt  >  2:710228/1‑149 (MQ=255)
                                                                                                                                                  ttATCGCCTGGATGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCATTAAATATGACAGCATAAACTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAt  >  1:922092/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAT  >  NC_000913/1870110‑1870404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: