Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,870,260 C→G 7.6% intergenic (+22/‑125) yeaH → / → yeaI UPF0229 family protein/putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,870,2600CG 7.6% 473.9 / 24.4 145intergenic (+22/‑125)yeaH/yeaIUPF0229 family protein/putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (76/58);  new base G (9/2);  total (85/60)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

Only 100 of 147 total aligned reads displayed.

GCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTC  >  NC_000913/1870112‑1870407
                                                                                                                                                    |                                                                                                                                                     
gCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAATCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATc                                                                                                                                                       >  1:482555/1‑149 (MQ=255)
gCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATc                                                                                                                                                       >  1:1051065/1‑149 (MQ=255)
 cGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCg                                                                                                                                                      <  1:201107/149‑1 (MQ=255)
  gagaATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGc                                                                                                                                                     >  1:816164/1‑149 (MQ=255)
    gaATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCt                                                                                                                                                   <  2:1522352/149‑1 (MQ=255)
     aaTATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTg                                                                                                                                                  <  1:485900/149‑1 (MQ=255)
       tatGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGCGATAATGACCTGGc                                                                                                                                                <  2:173885/149‑1 (MQ=255)
         tGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCATGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTg                                                                                                                                              >  1:1149178/1‑149 (MQ=255)
            gCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAt‑‑t                                                                                                                                         >  2:574276/1‑149 (MQ=255)
              aTCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAt‑‑ttt                                                                                                                                       <  2:658654/149‑1 (MQ=255)
              aTCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAt‑‑ttt                                                                                                                                       <  1:359835/149‑1 (MQ=255)
                   cAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCt                                                                                                                                  >  2:959353/1‑149 (MQ=255)
                   cAATCTACTTTCGACAAATTAGCGATGCACGACATCCGCGACCAGTATGATATTTATCCGGTGTTCCGTGAACTGATTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGCTCATTATCGCCTGGCTGAT‑‑TTTTAGCt                                                                                                                                  <  2:1582203/149‑1 (MQ=255)
                     aTCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTa                                                                                                                                >  1:1099501/1‑149 (MQ=255)
                      tCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTAc                                                                                                                               >  1:1364059/1‑149 (MQ=255)
                           tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTaa                                                                                                                          >  1:1207514/1‑149 (MQ=255)
                           tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTaa                                                                                                                          >  1:1495907/1‑149 (MQ=255)
                             tCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAAt                                                                                                                        <  2:210273/149‑1 (MQ=255)
                                 cAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTAtc                                                                                                                    <  1:204109/149‑1 (MQ=255)
                                    cTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCa                                                                                                                 >  1:406957/1‑149 (MQ=255)
                                          gATGCAGCACATCCGCTACCATGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                           <  1:584328/149‑1 (MQ=255)
                                          gATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                           <  1:301412/149‑1 (MQ=255)
                                          gATGCAGCACATCCGCGACAAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACAATCAGCCAGGTCATTTTCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                           >  2:1264017/1‑149 (MQ=255)
                                           aTGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatta                                                                                                          <  2:1349054/149‑1 (MQ=255)
                                              cagcaCATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtacat                                                                                                       >  2:1412969/1‑149 (MQ=255)
                                                gcaCATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtacatac                                                                                                     <  2:701301/149‑1 (MQ=255)
                                                 cacaTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTTTCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtacataca                                                                                                    >  2:163053/1‑149 (MQ=255)
                                                   caTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACAtt                                                                                                  <  2:1397079/149‑1 (MQ=255)
                                                    aTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCGAGGTCATTATCGCCTGGATGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTa                                                                                                 >  1:26829/1‑149 (MQ=255)
                                                    aTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTa                                                                                                 >  1:51389/1‑149 (MQ=255)
                                                    aTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGATAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGATTACTGTAAATTATCTCATTTATTacatacatga                                                                                                 >  1:26832/1‑147 (MQ=255)
                                                     tCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAg                                                                                                >  1:923310/1‑149 (MQ=255)
                                                     tCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAg                                                                                                <  1:1044792/149‑1 (MQ=255)
                                                       cgcgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCt                                                                                              <  1:1490745/149‑1 (MQ=255)
                                                       cgcgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCt                                                                                              >  2:166898/1‑149 (MQ=255)
                                                         cgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTa                                                                                            <  1:1590179/149‑1 (MQ=255)
                                                           aCCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTAAATACATTAGCTTACa                                                                                          >  1:566165/1‑149 (MQ=255)
                                                                gatgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCga                                                                                     >  2:351583/1‑148 (MQ=255)
                                                                gatgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGc                                                                                     >  1:94717/1‑149 (MQ=255)
                                                                gatgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGc                                                                                     <  2:621399/149‑1 (MQ=255)
                                                                 atgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCt                                                                                    <  2:27247/149‑1 (MQ=255)
                                                                  tgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCtt                                                                                   >  1:1253895/1‑149 (MQ=255)
                                                                     tatTTATCCGGTGTTCCGTGAACTGTTTAATATACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTAATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaa                                                                                <  1:764935/149‑1 (MQ=255)
                                                                         tATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAatat                                                                            <  2:835459/149‑1 (MQ=255)
                                                                           tCCGGTGTTCCGTGATCTGTTTCATATACATAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGGCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATCTATAACATACATTAGCTTACTATCGCTTAAAATATGa                                                                          <  2:195903/149‑1 (MQ=255)
                                                                           tCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGa                                                                          <  1:1488876/149‑1 (MQ=255)
                                                                            ccGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGAc                                                                         <  2:512956/149‑1 (MQ=255)
                                                                             cGGTGTTCCATGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACa                                                                        <  2:1149178/149‑1 (MQ=255)
                                                                                  ttCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATa                                                                   >  2:359024/1‑149 (MQ=255)
                                                                                     cGTTAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGTCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATTACAGCATAAcc                                                                <  1:1594321/149‑1 (MQ=255)
                                                                                       tGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCtt                                                              <  1:814825/149‑1 (MQ=255)
                                                                                       tGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCtt                                                              >  2:1548010/1‑149 (MQ=255)
                                                                                        gAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCttt                                                             >  1:858494/1‑149 (MQ=255)
                                                                                         aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                            <  1:1229275/149‑1 (MQ=255)
                                                                                         aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                            <  2:1099501/149‑1 (MQ=255)
                                                                                              tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                       >  1:1050806/1‑149 (MQ=255)
                                                                                              tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                       >  2:1582503/1‑149 (MQ=255)
                                                                                                  aTAAACAAAATGCAACAGCTAAAGGCTAAAACTATTAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTAAAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTa                                                   >  1:1279933/1‑149 (MQ=255)
                                                                                                  aTAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCACATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATACCCTTTACATAATTTa                                                   >  1:116440/1‑149 (MQ=255)
                                                                                                    aaaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGt                                                 >  1:1376965/1‑149 (MQ=255)
                                                                                                     aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGGCAGCATAACCTTTACATAATTTAGtt                                                >  2:787703/1‑149 (MQ=255)
                                                                                                     aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt                                                >  2:898274/1‑149 (MQ=255)
                                                                                                     aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt                                                >  2:1579797/1‑149 (MQ=255)
                                                                                                             gCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGaaaa                                        <  1:567118/149‑1 (MQ=255)
                                                                                                                aCAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACaa                                     <  2:419073/149‑1 (MQ=255)
                                                                                                                  aGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATc                                   >  2:729709/1‑149 (MQ=255)
                                                                                                                    cTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAt                                 >  2:1098994/1‑149 (MQ=255)
                                                                                                                    cTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAt                                 >  1:636865/1‑149 (MQ=255)
                                                                                                                     tAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAtt                                <  1:959353/149‑1 (MQ=255)
                                                                                                                      aaaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTc                               >  2:211553/1‑149 (MQ=255)
                                                                                                                      aaaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTc                               <  1:574276/149‑1 (MQ=255)
                                                                                                                      aaaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTc                               <  1:685289/149‑1 (MQ=255)
                                                                                                                       aatgCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg                              <  2:967177/146‑1 (MQ=255)
                                                                                                                         ggCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGa                            >  2:1261450/1‑149 (MQ=255)
                                                                                                                          gCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTTCAGACATTAGCTTACAATCGCTTGAAATATGACAGCATCACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaa                           >  2:104066/1‑149 (MQ=255)
                                                                                                                          gCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaa                           >  1:1518833/1‑149 (MQ=255)
                                                                                                                           cTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaa                          >  2:1153639/1‑149 (MQ=255)
                                                                                                                             ctaaaaaTCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaaa                        >  1:1126701/7‑149 (MQ=39)
                                                                                                                              aaaCTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAAt                       >  2:1293118/1‑149 (MQ=255)
                                                                                                                               aaaaaTCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATAGCTTTAAATATTACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATAATTCGGAAAAATg                      >  2:1030066/5‑149 (MQ=255)
                                                                                                                                  tATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTATACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt                   >  2:848308/1‑149 (MQ=255)
                                                                                                                                  tATCAGCCAGGTAATTATCGCCTGGCTGAT‑‑TTTTAGCTTAATGTAAATTATATCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGTAAAATGAtt                   <  2:260880/149‑1 (MQ=255)
                                                                                                                                   aTCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtta                  >  1:1473284/1‑148 (MQ=255)
                                                                                                                                    ttaGCCAGGCGATAATGACCTGGCTGATAGTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt                   <  2:896339/147‑1 (MQ=38)
                                                                                                                                    tCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  1:1343909/1‑149 (MQ=255)
                                                                                                                                    tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  2:545868/1‑149 (MQ=255)
                                                                                                                                    tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  1:994537/1‑149 (MQ=255)
                                                                                                                                    tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  1:630228/1‑149 (MQ=255)
                                                                                                                                    tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  1:869564/1‑149 (MQ=255)
                                                                                                                                     cAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAg                <  2:12933/149‑1 (MQ=255)
                                                                                                                                      aGCCAGGACATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGt               >  1:406840/1‑149 (MQ=255)
                                                                                                                                       gCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc              <  2:636865/149‑1 (MQ=255)
                                                                                                                                       gCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc              >  2:547960/1‑149 (MQ=255)
                                                                                                                                        ccAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCa             >  2:429776/1‑149 (MQ=255)
                                                                                                                                            gTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAacac         <  1:1298993/149‑1 (MQ=255)
                                                                                                                                               aTTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTa      >  2:601474/1‑149 (MQ=255)
                                                                                                                                               aTTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTa      >  2:1308371/1‑149 (MQ=255)
                                                                                                                                                ttATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAt     >  2:710228/1‑149 (MQ=255)
                                                                                                                                                ttATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAt     >  2:505445/1‑149 (MQ=255)
                                                                                                                                                ttATCGCCTGGATGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCATTAAATATGACAGCATAAACTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAt     >  1:922092/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTC  >  NC_000913/1870112‑1870407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: