Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,870,261 G→A 7.6% intergenic (+23/‑124) yeaH → / → yeaI UPF0229 family protein/putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,870,2610GA 7.6% 441.0 / 15.4 144intergenic (+23/‑124)yeaH/yeaIUPF0229 family protein/putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (75/57);  new base A (9/2);  total (85/59)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 145 total aligned reads displayed.

CGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTC  >  NC_000913/1870113‑1870407
                                                                                                                                                    |                                                                                                                                                    
cGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCg                                                                                                                                                      <  1:201107/149‑1 (MQ=255)
 gagaATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGc                                                                                                                                                     >  1:816164/1‑149 (MQ=255)
   gaATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCt                                                                                                                                                   >  2:170352/1‑149 (MQ=255)
   gaATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCt                                                                                                                                                   <  2:1522352/149‑1 (MQ=255)
    aaTATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTg                                                                                                                                                  <  1:485900/149‑1 (MQ=255)
      tatGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGCGATAATGACCTGGc                                                                                                                                                <  2:173885/149‑1 (MQ=255)
      tatGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCAGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGACTAAAACTATCAGCCAGGTCATTATCGCCTggg                                                                                                                                                >  1:248138/1‑148 (MQ=255)
                  cAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCt                                                                                                                                  >  2:959353/1‑149 (MQ=255)
                    aTCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACACCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTa                                                                                                                                <  1:847430/149‑1 (MQ=255)
                     tCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTAc                                                                                                                               >  1:1364059/1‑149 (MQ=255)
                      cTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGTATGATATTTATCCGGGGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACt                                                                                                                              <  1:1495862/149‑1 (MQ=255)
                          tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCTCCTGGCTTAT‑‑TTTTAGCTTACAGTaa                                                                                                                          >  2:127961/1‑149 (MQ=255)
                          tttCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTaa                                                                                                                          >  1:908030/1‑149 (MQ=255)
                            tCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAAt                                                                                                                        <  2:210273/149‑1 (MQ=255)
                              gACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTa                                                                                                                      >  2:204109/1‑149 (MQ=255)
                                 aaCTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTAtct                                                                                                                   >  2:685289/1‑149 (MQ=255)
                                   cTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCa                                                                                                                 >  1:1582659/1‑149 (MQ=255)
                                    tttGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCAt                                                                                                                >  1:1349054/1‑149 (MQ=255)
                                    tttGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCAt                                                                                                                >  1:12933/1‑149 (MQ=255)
                                        cGATTCAGCACATCCGCTACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttat                                                                                                            <  2:248138/149‑1 (MQ=255)
                                         gATGCAGCACATCCGCTACCATGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                           <  1:584328/149‑1 (MQ=255)
                                         gATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                           <  1:301412/149‑1 (MQ=255)
                                         gATGCAGCACATCCGCGACAAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACAATCAGCCAGGTCATTTTCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatt                                                                                                           >  2:1264017/1‑149 (MQ=255)
                                          aTGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATttatta                                                                                                          <  2:1349054/149‑1 (MQ=255)
                                               gcaCATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATtacatac                                                                                                     <  2:701301/149‑1 (MQ=255)
                                                  caTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACAtt                                                                                                  <  2:1397079/149‑1 (MQ=255)
                                                   aTCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCGAGGTCATTATCGCCTGGATGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTa                                                                                                 >  1:26829/1‑149 (MQ=255)
                                                   aTCCGCGACCAGGATGATATTTATCCGGTGTTCCGGGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTCTCAGCCAGGGCATTATCGCCTGTCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTa                                                                                                 <  1:1203488/149‑1 (MQ=255)
                                                    tCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAg                                                                                                >  1:923310/1‑149 (MQ=255)
                                                    tCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAg                                                                                                <  1:1044792/149‑1 (MQ=255)
                                                      cgcgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCt                                                                                              >  2:166898/1‑149 (MQ=255)
                                                      cgcgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCt                                                                                              <  1:1490745/149‑1 (MQ=255)
                                                        cgACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTa                                                                                            <  2:1213953/149‑1 (MQ=255)
                                                          aCCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTAAATACATTAGCTTACa                                                                                          >  1:566165/1‑149 (MQ=255)
                                                               gatgatATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCga                                                                                     >  2:351583/1‑148 (MQ=255)
                                                                    tatTTATCCGGTGTTCCGTGAACTGTTTAATATACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTAATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaa                                                                                <  1:764935/149‑1 (MQ=255)
                                                                     atTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaaa                                                                               <  2:1388693/149‑1 (MQ=255)
                                                                     atTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTaaa                                                                               <  2:332563/149‑1 (MQ=255)
                                                                      tttATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGTCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAat                                                                              <  2:631568/149‑1 (MQ=255)
                                                                        tATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAatat                                                                            <  2:835459/149‑1 (MQ=255)
                                                                          tCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGa                                                                          >  1:1432798/1‑149 (MQ=255)
                                                                           ccGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGAc                                                                         <  2:512956/149‑1 (MQ=255)
                                                                                 ttCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATa                                                                   >  1:491810/1‑149 (MQ=255)
                                                                                    cGTTAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGTCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATTACAGCATAAcc                                                                <  1:1594321/149‑1 (MQ=255)
                                                                                      tGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCtt                                                              <  1:814825/149‑1 (MQ=255)
                                                                                        aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                            <  1:1229275/149‑1 (MQ=255)
                                                                                        aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                            <  2:593740/149‑1 (MQ=255)
                                                                                        aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                            >  2:1298993/1‑149 (MQ=255)
                                                                                        aaCTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTa                                                            <  2:1099501/149‑1 (MQ=255)
                                                                                             tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                       >  2:1498517/1‑149 (MQ=255)
                                                                                             tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                       >  1:1050806/1‑149 (MQ=255)
                                                                                             tttCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATaa                                                       >  2:1582503/1‑149 (MQ=255)
                                                                                                 aTAAACAAAATGCAACAGCTAAAGGCTAAAACTATTAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTAAAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTa                                                   >  1:1279933/1‑149 (MQ=255)
                                                                                                 aTAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCACATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATACCCTTTACATAATTTa                                                   >  1:116440/1‑149 (MQ=255)
                                                                                                  tAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAg                                                  <  1:170352/149‑1 (MQ=255)
                                                                                                    aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGGCAGCATAACCTTTACATAATTTAGtt                                                >  2:787703/1‑149 (MQ=255)
                                                                                                    aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt                                                >  2:1579797/1‑149 (MQ=255)
                                                                                                    aaCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGtt                                                >  2:898274/1‑149 (MQ=255)
                                                                                                     aCAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTc                                               >  1:1057133/1‑149 (MQ=255)
                                                                                                            gCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGaaaa                                        <  1:567118/149‑1 (MQ=255)
                                                                                                               aCAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACaa                                     <  2:419073/149‑1 (MQ=255)
                                                                                                                 aGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATc                                   >  2:729709/1‑149 (MQ=255)
                                                                                                                   cTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAt                                 >  1:636865/1‑149 (MQ=255)
                                                                                                                    tAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCAtt                                <  1:959353/149‑1 (MQ=255)
                                                                                                                    tAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCAAGAAAACAATCAtt                                >  1:442413/1‑149 (MQ=255)
                                                                                                                     aaaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAAGAATCATTc                               <  1:1129050/149‑1 (MQ=255)
                                                                                                                     aaaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTc                               <  1:574276/149‑1 (MQ=255)
                                                                                                                     aaaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTc                               <  1:685289/149‑1 (MQ=255)
                                                                                                                      aatgCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg                              <  2:967177/146‑1 (MQ=255)
                                                                                                                      aaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg                              >  1:1233286/1‑149 (MQ=255)
                                                                                                                      aaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCg                              >  2:404165/1‑149 (MQ=255)
                                                                                                                      aaGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACATTTACATAATTTAGTTCCAGAAAACAATCATTCg                              >  1:987265/1‑149 (MQ=255)
                                                                                                                         gCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTTCAGACATTAGCTTACAATCGCTTGAAATATGACAGCATCACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaa                           >  2:104066/1‑149 (MQ=255)
                                                                                                                         gCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaa                           >  1:1518833/1‑149 (MQ=255)
                                                                                                                          cTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaa                          >  2:1153639/1‑149 (MQ=255)
                                                                                                                           tAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaa                         >  2:469305/1‑149 (MQ=255)
                                                                                                                            ctaaaaaTCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaaa                        >  1:1126701/7‑149 (MQ=39)
                                                                                                                             taaaaaTCAGCCAGGCGATAATGACCTGGCTGATAGTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGaaaa                         >  1:936654/6‑149 (MQ=37)
                                                                                                                             aaaCTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAAt                       >  2:1045142/1‑149 (MQ=255)
                                                                                                                              aaaaaTCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATAGCTTTAAATATTACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATAATTCGGAAAAATg                      >  2:1030066/5‑149 (MQ=255)
                                                                                                                                 tATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt                   <  2:1555455/149‑1 (MQ=255)
                                                                                                                                 tATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTATACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt                   >  2:848308/1‑149 (MQ=255)
                                                                                                                                 tATCAGCCAGGTAATTATCGCCTGGCTGAT‑‑TTTTAGCTTAATGTAAATTATATCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGTAAAATGAtt                   <  2:260880/149‑1 (MQ=255)
                                                                                                                                  aTCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtta                  >  1:1473284/1‑148 (MQ=255)
                                                                                                                                   ttaGCCAGGCGATAATGACCTGGCTGATAGTTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGAtt                   <  2:896339/147‑1 (MQ=38)
                                                                                                                                   tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  2:545868/1‑149 (MQ=255)
                                                                                                                                   tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  1:869564/1‑149 (MQ=255)
                                                                                                                                   tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  1:630228/1‑149 (MQ=255)
                                                                                                                                   tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  2:1120205/1‑149 (MQ=255)
                                                                                                                                   tCAGCCAGGCGATAATGACCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCa                 >  1:301196/1‑149 (MQ=255)
                                                                                                                                    cAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAg                <  2:12933/149‑1 (MQ=255)
                                                                                                                                     aGCCAGGACATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGt               >  1:406840/1‑149 (MQ=255)
                                                                                                                                      gCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc              >  1:361244/1‑149 (MQ=255)
                                                                                                                                      gCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc              <  2:636865/149‑1 (MQ=255)
                                                                                                                                      gCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTc              >  2:547960/1‑149 (MQ=255)
                                                                                                                                         aGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAac           <  2:1582659/149‑1 (MQ=255)
                                                                                                                                           gTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAacac         <  1:1298993/149‑1 (MQ=255)
                                                                                                                                              aTTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTa      >  2:601474/1‑149 (MQ=255)
                                                                                                                                               ttATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAt     >  2:710228/1‑149 (MQ=255)
                                                                                                                                               ttATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTAt     >  2:505445/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCGCCTGGCTGAT‑‑TTTTAGCTTACTGTAAATTATCTCATTTATTACATACATTAGCTTACAATCGCTTTAAATATGACAGCATAACCTTTACATAATTTAGTTCCAGAAAACAATCATTCGGAAAAATGATTCAGTCAACACGTATTTC  >  NC_000913/1870113‑1870407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: