Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,885,805 T→A 7.5% intergenic (+16/‑40) yeaW → / → yeaX putative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster/putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,885,8050TA 7.5% 255.5 / 8.4 89intergenic (+16/‑40)yeaW/yeaXputative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster/putative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand):  ref base T (35/46);  new base A (4/3);  total (39/50)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

GTACAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGT  >  NC_000913/1885658‑1885953
                                                                                                                                                   |                                                                                                                                                    
gTACAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACTTGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTa                                                                                                                                                     >  1:63065/1‑149 (MQ=255)
gTACAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGTGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTa                                                                                                                                                     <  2:1224014/149‑1 (MQ=255)
gTACAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTa                                                                                                                                                     >  2:1387391/1‑149 (MQ=255)
   cAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTAttt                                                                                                                                                  >  2:293896/1‑149 (MQ=255)
    aGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTAtttt                                                                                                                                                 >  2:492561/1‑149 (MQ=255)
      aaaGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTcc                                                                                                                                               >  1:1065638/1‑149 (MQ=255)
        aGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTccgc                                                                                                                                             >  1:265940/1‑149 (MQ=255)
            cTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGAAAATACcgccgcc                                                                                                                                         <  2:458810/149‑2 (MQ=255)
                 aTCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCGATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCt                                                                                                                                    <  1:91463/149‑1 (MQ=255)
                      gTGGCTATCGTGGGCAGGGGCGCAGCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGAttt                                                                                                                               <  2:1419007/149‑1 (MQ=255)
                          cTATCTTGGGCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGTCATTTACGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGa                                                                                                                           <  1:530852/149‑1 (MQ=255)
                          cTATCGTGGTCAGGGGCGCATCATGGCCGACACTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCAATAATCTGCTCTCGCATGTGTTTAAGGACTAATGACATCGGCGTCGGTATTTTCCGCCGCTGGGCTGATTTTTGa                                                                                                                           <  2:677842/149‑1 (MQ=255)
                           tATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAt                                                                                                                          <  2:829194/149‑1 (MQ=255)
                             tCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCAGAATCTGCTCGCGCAGGTGGTTAAGGACAAATGACCTCGGCGGCGGAGTTTTCCGCCGCTGGGCTGATTATTTATgg                                                                                                                        >  1:316902/1‑149 (MQ=255)
                               gTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGAGGGAg                                                                                                                      <  1:86014/149‑1 (MQ=255)
                                 ggTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTa                                                                                                                    <  2:908171/149‑1 (MQ=255)
                                    cAGGGGCGCATCATGGCCGAGAGTAGCGGTTGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAg                                                                                                                 <  2:344060/149‑1 (MQ=255)
                                    cAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAg                                                                                                                 <  1:1307539/149‑1 (MQ=255)
                                            catcatGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCa                                                                                                         <  2:1440713/149‑1 (MQ=255)
                                              tcatGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGa                                                                                                       <  2:886893/149‑1 (MQ=255)
                                              tcatGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGa                                                                                                       <  1:749491/149‑1 (MQ=255)
                                              gcatGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCTTTTCCATAATCTGCTGTCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGa                                                                                                       <  1:862021/148‑1 (MQ=255)
                                                   gCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCTGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATc                                                                                                  <  2:1046416/149‑1 (MQ=255)
                                                      gACAGTAGCGGTAGTGGCATTTACGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCaaa                                                                                               <  2:903199/149‑1 (MQ=255)
                                                      gACAGTAGCGGGAGTGGCATTTCCGAACTAGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTACTGACATCGGCGGCGGTTTTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCaaa                                                                                               <  2:672986/149‑1 (MQ=255)
                                                          gTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGtt                                                                                           <  1:1324292/149‑1 (MQ=255)
                                                           tAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGttt                                                                                          >  2:1398171/1‑149 (MQ=255)
                                                           tAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGttt                                                                                          >  1:1407490/1‑149 (MQ=255)
                                                             gCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGtt                                                                                           >  1:989830/1‑146 (MQ=255)
                                                             gCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGtt                                                                                           <  2:989830/146‑1 (MQ=255)
                                                             gCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGa                                                                                        >  2:1232612/1‑149 (MQ=255)
                                                             gCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGa                                                                                        >  2:294047/1‑149 (MQ=255)
                                                              cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACCTCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                       <  2:1075649/149‑1 (MQ=255)
                                                              cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCTGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                       <  1:1421731/149‑1 (MQ=255)
                                                              cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                       <  2:354850/149‑1 (MQ=255)
                                                              cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                       <  2:521918/149‑1 (MQ=255)
                                                              cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                       <  2:63065/149‑1 (MQ=255)
                                                              cGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGAGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGaa                                                                                       >  2:96269/1‑149 (MQ=255)
                                                               ggTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAg                                                                                      <  1:492561/149‑1 (MQ=255)
                                                                gTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGt                                                                                     >  2:512081/1‑149 (MQ=255)
                                                                gTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGt                                                                                     >  1:587971/1‑149 (MQ=255)
                                                                gTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGt                                                                                     >  2:926664/1‑149 (MQ=255)
                                                                    tGGCATTTCCGAACATGGGATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCAGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAg                                                                                 >  2:104241/1‑149 (MQ=255)
                                                                      gCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGt                                                                               <  2:162686/149‑1 (MQ=255)
                                                                      gCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGt                                                                               >  2:1556955/1‑149 (MQ=255)
                                                                        aTTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGa                                                                             >  2:1391651/1‑149 (MQ=255)
                                                                             agtaCATGGTATCGCACATTTCCATAATCTGCTTGCGCAGGTTTTTAATGACTAATGACATCTGCGGCGGTATTTTCCGCCGCTGGTCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACATGTGAGCCAg                                                                        <  1:462450/146‑1 (MQ=255)
                                                                               aaCATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGt                                                                      <  2:265940/149‑1 (MQ=255)
                                                                                aCATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGtt                                                                     <  2:1509397/149‑1 (MQ=255)
                                                                                   tGGTATCGCCCATTTCCATAATCTGCTGGCTCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGaa                                                                  <  2:1122735/149‑1 (MQ=255)
                                                                                    ggTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAAc                                                                 <  1:293896/149‑1 (MQ=255)
                                                                                      tATCGCCCATTTCCATCATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAAccc                                                               <  2:530384/149‑1 (MQ=255)
                                                                                       aTCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGTCTGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAAcccc                                                              <  2:909205/149‑1 (MQ=255)
                                                                                       aTCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAAcccc                                                              <  2:1531878/149‑1 (MQ=255)
                                                                                        tCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCt                                                             <  1:1556955/149‑1 (MQ=255)
                                                                                          gCCCATTTCCATAAGCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTa                                                           <  2:378968/149‑1 (MQ=255)
                                                                                           cccATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTAc                                                          >  2:1178903/1‑149 (MQ=255)
                                                                                           cccATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTAc                                                          <  1:1398171/149‑1 (MQ=255)
                                                                                           cccATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTAc                                                          >  2:666263/1‑149 (MQ=255)
                                                                                           cccATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTAc                                                          >  2:736120/1‑149 (MQ=255)
                                                                                              aTTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTATCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGa                                                       >  1:399679/1‑149 (MQ=255)
                                                                                               tttCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGaa                                                      <  1:1309006/149‑1 (MQ=255)
                                                                                                ttCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTAAACGAAc                                                     >  2:1069463/1‑149 (MQ=255)
                                                                                                ttCCATAAGCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAAc                                                     <  2:98991/149‑1 (MQ=255)
                                                                                                   cATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAAACCCTTACCGAACAgg                                                  >  2:1040534/1‑149 (MQ=255)
                                                                                                    aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGt                                                 <  1:1178903/149‑1 (MQ=255)
                                                                                                    aTAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGt                                                 >  1:239055/1‑149 (MQ=255)
                                                                                                        tCTGCTGGCGCAGGTGCTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGaaa                                             <  1:1523102/149‑1 (MQ=255)
                                                                                                        tCTGCTGGCCCAGGTGTTTAAGGACTAATGACCTCGGCGGCGGGATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCATTGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGaaa                                             <  2:1018076/149‑1 (MQ=255)
                                                                                                                   aGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCt                                  >  1:658965/1‑149 (MQ=255)
                                                                                                                       gtTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtggtg                              <  1:104241/149‑1 (MQ=255)
                                                                                                                       gtTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCtggtg                              <  2:1065638/149‑1 (MQ=255)
                                                                                                                           aaGTACTAATGACATCGGCGGCGTTATTTTCAGCCGCAGGCCTGATTTATGATCGAGTACAGCAATGTCAGACTATCAAATGTAAGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACACGTGAAACGCATCACGCAGGTGGaaa                          >  2:371025/1‑149 (MQ=255)
                                                                                                                           aaGGACTAATGACATTGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCaa                          >  1:1450306/1‑149 (MQ=255)
                                                                                                                             ggACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGt                                                 <  2:193544/124‑1 (MQ=255)
                                                                                                                             ggACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGt                                                 >  1:193544/1‑124 (MQ=255)
                                                                                                                                  aaTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATgg                   <  1:736120/149‑1 (MQ=255)
                                                                                                                                  aaTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATgg                   >  1:237193/1‑149 (MQ=255)
                                                                                                                                   aTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGc                  >  2:140805/1‑149 (MQ=255)
                                                                                                                                   aTGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGc                  >  1:1359170/1‑149 (MQ=255)
                                                                                                                                     gACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCaa                >  2:1500535/1‑149 (MQ=255)
                                                                                                                                       cATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTCATGGAGTACAGCAATGTCAGAATATCAAAGGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGATCAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAAc              <  2:82104/149‑1 (MQ=255)
                                                                                                                                        cccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAg                                                                            >  2:1478312/5‑86 (MQ=38)
                                                                                                                                        cccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAg                                                                            <  1:1478312/82‑1 (MQ=38)
                                                                                                                                        cccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAg                                                                            <  1:1216150/82‑1 (MQ=38)
                                                                                                                                        cccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAg                                                                            >  2:1216150/5‑86 (MQ=38)
                                                                                                                                         ccagcggcggAAAATACCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCa            >  1:1574983/4‑149 (MQ=255)
                                                                                                                                                cggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTg     >  2:1100373/1‑149 (MQ=255)
                                                                                                                                                 ggTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGc    >  2:447505/1‑149 (MQ=255)
                                                                                                                                                   tATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGt  <  2:1450306/149‑1 (MQ=255)
                                                                                                                                                   tATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGt  <  1:666263/149‑1 (MQ=255)
                                                                                                                                                   tATTTTCCGCCGCGGGGCTGATTTTTGATGAAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGt  <  2:676231/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTACAGAAAGGGCTGAAATCGCGTGGCTATCGTGGTCAGGGGCGCATCATGGCCGACAGTAGCGGTAGTGGCATTTCCGAACATGGTATCGCCCATTTCCATAATCTGCTGGCGCAGGTGTTTAAGGACTAATGACATCGGCGGCGGTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGT  >  NC_000913/1885658‑1885953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: