Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,996,373 T→A 6.9% M154L (ATG→TTG)  sdiA ← quorum‑sensing transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,996,3730TA 6.9% 269.4 / 5.9 91M154L (ATG→TTG) sdiAquorum‑sensing transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (41/43);  new base A (3/4);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGC  >  NC_000913/1996226‑1996521
                                                                                                                                                   |                                                                                                                                                    
tcatcGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAtt                                                                                                                                                     >  1:558609/1‑149 (MQ=255)
     gCTATCTCTGCTGATGTTTTCCCTTCCGCCGGCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTaa                                                                                                                                                <  2:1518927/149‑1 (MQ=255)
        aTCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTg                                                                                                                                             <  1:1021474/149‑1 (MQ=255)
        aTCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTg                                                                                                                                             <  2:643686/149‑1 (MQ=255)
          ctctGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCa                                                                                                                                           <  1:1043625/149‑1 (MQ=255)
          ctctGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCa                                                                                                                                           <  2:996670/149‑1 (MQ=255)
           tctGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCGTGCTGAAATTCATCTCTGGCGTCATCACTAGTTCATCATTAAACCGCATCAGAGCCATCAGACTTTCGCGCACCAGTATCTCCATTTTTAATTGCAg                                                                                                                                          >  2:195838/1‑149 (MQ=255)
            ctgctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGt                                                                                                                                         <  1:123490/149‑1 (MQ=255)
            ctgctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGt                                                                                                                                         <  2:340907/149‑1 (MQ=255)
              gctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAATTAAAAATGCAGtta                                                                                                                                       <  1:1493150/149‑2 (MQ=255)
               ctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtca                                                                                                                                      >  1:1240979/1‑149 (MQ=255)
               ctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAATTAAAAATGCAGttac                                                                                                                                      <  2:227223/149‑3 (MQ=255)
               ctgATGTTTTCCCTTACGCCTTACACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGTCTTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAATTAAAAATGCAGttac                                                                                                                                      <  2:667421/149‑3 (MQ=255)
                  aTGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtcatca                                                                                                                                   >  2:373476/1‑149 (MQ=255)
                  aTGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAATTAAAAATGCAGttactgg                                                                                                                                   <  1:396484/149‑6 (MQ=255)
                     ttttCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTa                                                                                                                                >  1:576034/1‑149 (MQ=255)
                         cccTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGaa                                                                                                                            <  1:545946/149‑1 (MQ=255)
                           cTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATg                                                                                                                          >  1:1362105/1‑149 (MQ=255)
                           cTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATg                                                                                                                          <  2:621340/149‑1 (MQ=255)
                            tttctgcgTGCAACTCAGAATTTCTTTTTCGCTCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCATAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTTCAGTTCATCACTAAGAATgg                                                                                                                         <  2:351746/143‑1 (MQ=255)
                            ttCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATgg                                                                                                                         >  1:1044015/1‑149 (MQ=255)
                            ttCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATgg                                                                                                                         <  2:249379/149‑1 (MQ=255)
                              ccgccgTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCCTCAGACTTTCGCGAACCAGTAACTGCATTTATAATTGCAGTTCATCACTAAGAATGGGt                                                                                                                       >  1:1082549/1‑149 (MQ=255)
                              ccgccgTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGt                                                                                                                       >  2:528722/1‑149 (MQ=255)
                              ccgccgTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGt                                                                                                                       >  1:1416421/1‑149 (MQ=255)
                                   gTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTc                                                                                                                  <  2:1386835/149‑1 (MQ=255)
                                          tCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcgcgcg                                                                                                           >  2:869674/1‑149 (MQ=255)
                                          tCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcgcgcg                                                                                                           >  2:1584483/1‑149 (MQ=255)
                                            aGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCt                                                                                                         <  2:364197/149‑1 (MQ=255)
                                             gAATTTCTTTTTCGCGCTGGCTGAAATTCATCTCTGGCGGCATCACTATATCATCATTTAAACGCATCAGCGCCATAAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTg                                                                                                        <  2:320325/149‑1 (MQ=255)
                                              aaTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGc                                                                                                       >  1:1197014/1‑149 (MQ=255)
                                              aaTTTCTTTTTCGCGCTTGCTGAAAATCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCCTCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGc                                                                                                       <  1:119606/149‑1 (MQ=255)
                                               aTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCa                                                                                                      >  1:1312839/1‑149 (MQ=255)
                                                  tCTTTTTCGAGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCGGCAACg                                                                                                   <  1:818594/149‑1 (MQ=255)
                                                   cTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACgg                                                                                                  <  1:1055301/149‑1 (MQ=255)
                                                            gcTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGAc                                                                                         >  1:418236/1‑149 (MQ=255)
                                                                 cTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACaaaaa                                                                                    <  1:1551039/149‑1 (MQ=255)
                                                                  tGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAg                                                                                   >  2:719421/1‑149 (MQ=255)
                                                                    aaaTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAAATGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCACCGGGAAAAGGACAAAACGcc                                                                                 >  2:991846/1‑149 (MQ=255)
                                                                      aTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGTGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCa                                                                               <  2:901383/149‑1 (MQ=255)
                                                                            ctctGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGccc                                                                         >  1:7722/1‑149 (MQ=255)
                                                                              ctGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCgg                                                                       <  1:551982/149‑1 (MQ=255)
                                                                               tGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGCCAAAAAGCCCAGCGCCCGGt                                                                      >  2:6436/1‑149 (MQ=255)
                                                                               tGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGt                                                                      >  2:540392/1‑149 (MQ=255)
                                                                               tGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTCCATTTTTAATTGCAGTTCATCACAAAGAATAGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCgga                                                                      >  2:1083857/1‑148 (MQ=255)
                                                                                ggCGTCATCACTATTTCATCATTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGttt                                                                    <  1:490940/149‑1 (MQ=255)
                                                                                ggCGTCATAACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTAATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGtt                                                                     <  2:1001169/149‑1 (MQ=255)
                                                                                 gCGTCATCAATATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTAAATTGCAGATCATCACTAAGACTGCGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGttt                                                                    <  1:259201/149‑1 (MQ=255)
                                                                                  cGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTg                                                                   <  2:1416421/149‑1 (MQ=255)
                                                                                   gTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTgg                                                                  <  2:750180/149‑1 (MQ=255)
                                                                                   gTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGATCATCACTAAGAATGCGGATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCgggtggg                                                                  <  2:1095465/149‑6 (MQ=255)
                                                                                     catcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGgca                                                                <  2:1312839/149‑1 (MQ=255)
                                                                                      atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGgcag                                                               <  2:891116/149‑1 (MQ=255)
                                                                                      atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGgcag                                                               >  1:304987/1‑149 (MQ=255)
                                                                                       tcaCTATTTCATAATTTAAACTCCTCAGCGCCATCAGACATTAGCGCGCCACTAACTGCATTTTTAATTGCAGTTCAACACTAAGAATGGGTATTTCTCCCTCGCACCAACGGGAAAAGCCCAAAAAGCCCAGCGACCGGATTCgcagc                                                              >  2:1289672/1‑149 (MQ=255)
                                                                                         aCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGTAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCAt                                                            >  2:733630/1‑149 (MQ=255)
                                                                                          cTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCAtt                                                           >  2:1269489/1‑149 (MQ=255)
                                                                                             tttCATCATTTCTACGCATCAGAGCCATCAGACTTTCGCGAACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCGGCAACGGGAAAAGGACAAAACGCCCAGCGCCCGGTTTGGCAGCATTaaa                                                        <  1:927448/149‑1 (MQ=255)
                                                                                             tttCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTaaa                                                        <  1:686130/149‑1 (MQ=255)
                                                                                             tttCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTaaa                                                        <  1:1058236/149‑1 (MQ=255)
                                                                                             tttCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTaaa                                                        <  1:373476/149‑1 (MQ=255)
                                                                                                 atcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACt                                                    >  1:1434976/1‑149 (MQ=255)
                                                                                                  tcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTg                                                   >  1:1439658/1‑149 (MQ=255)
                                                                                                   catTTAAACGCATCAGAGCCATCAGACTTTCGCGCAGCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGa                                                  <  2:233470/149‑1 (MQ=255)
                                                                                                    atTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAATGCCACGCGCCCGGTTTGGCAGCATTAAATACTGAg                                                 >  2:143013/1‑149 (MQ=255)
                                                                                                    atTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACAGCAATTTTAATTGCAGTTCAACACTAAGAATGGGAATTTCGCGCGCGCTGCAACGGGAAAACCACAAAAAGCCAAGCGCCCGCTTTGGCAGCATTAAAAACTGAg                                                 >  1:486846/1‑149 (MQ=255)
                                                                                                     tttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGt                                                >  2:1035170/1‑149 (MQ=255)
                                                                                                     tttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGt                                                <  1:698772/149‑1 (MQ=255)
                                                                                                     tttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGt                                                <  1:829425/149‑1 (MQ=255)
                                                                                                     tttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGt                                                <  2:1362105/149‑1 (MQ=255)
                                                                                                     tttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCAATAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGt                                                >  2:305881/1‑149 (MQ=255)
                                                                                                        aaaaGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGAAATTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGac                                             <  1:1462651/149‑1 (MQ=255)
                                                                                                          aCGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGacac                                           >  2:826559/1‑149 (MQ=255)
                                                                                                              aTCAGAGCCATCAGACTTTCGAGCACCAGTAACTGCATTTTTAATTGCAGTTAATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACcgcg                                       >  2:1518722/1‑149 (MQ=255)
                                                                                                                     ccATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTaaa                                >  1:1094040/1‑149 (MQ=255)
                                                                                                                      cctCAGACTTTCGCGCACCAGTAACTGCATTTATAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAAc                               <  1:775512/147‑1 (MQ=255)
                                                                                                                      cATGAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAAc                               <  1:419064/149‑1 (MQ=255)
                                                                                                                       aTCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAAcc                              >  2:664564/1‑149 (MQ=255)
                                                                                                                        tCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCa                             >  2:589382/1‑149 (MQ=255)
                                                                                                                           gACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAtgt                          <  2:1240979/149‑1 (MQ=255)
                                                                                                                            aCTTTCGCTCACCAGTAACTGGATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGGGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAtgtg                         <  2:1082549/149‑1 (MQ=255)
                                                                                                                              tttCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTgcg                       >  1:1420913/1‑149 (MQ=255)
                                                                                                                                      aCCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGc               >  1:1440773/1‑149 (MQ=255)
                                                                                                                                         atgaaCTGCAATTAAAAATGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAACGCCCAGCGCCCGGTTTGGCAGCAGTAAATACTGAGTGACACCGCGGCGTCACCCCTGTGCGCGCGCGGCTTc            >  2:137839/4‑149 (MQ=255)
                                                                                                                                         atgaaCTGCAATTAAAAATGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTc            >  2:1353680/4‑149 (MQ=255)
                                                                                                                                         aGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTc            <  1:869674/149‑1 (MQ=255)
                                                                                                                                           tAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTccc          <  2:1044015/149‑1 (MQ=255)
                                                                                                                                             aCTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCAt        >  2:490521/1‑149 (MQ=255)
                                                                                                                                              cTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATa       >  2:1260829/1‑149 (MQ=255)
                                                                                                                                               tGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGACAAGCGCCAGGTTTGGCAGCATTAAATACTGAGTGACAACGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATaa      >  1:58431/1‑149 (MQ=255)
                                                                                                                                                gCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAAc     >  1:690770/1‑149 (MQ=255)
                                                                                                                                                 cATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCACCGGGAAAAGGCCAAAAAGCCCAGCGCCCGGTTTGGCAGCAGTAAAGACTGCGTGACACCGCGGCGTAGACCATGTGCGCGCGCGGCCTccaaacgcg    >  2:223664/1‑142 (MQ=255)
                                                                                                                                                   tttttAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACTAAAAGCCCAGCGCCGGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGGACACCAGGTGCGCGCGCGGCTTCCAATAACggg  >  2:1055357/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGC  >  NC_000913/1996226‑1996521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: