Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,012,153 T→A 5.8% pseudogene (199/312 nt) yedN ← pseudogene, IpaH/YopM family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,012,1530TA 5.8% 634.9 / 20.5 206pseudogene (199/312 nt)yedNpseudogene, IpaH/YopM family
Reads supporting (aligned to +/- strand):  ref base T (88/106);  new base A (7/5);  total (95/111)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 208 total aligned reads displayed.

TTATGCCGAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTA  >  NC_000913/2012006‑2012301
                                                                                                                                                   |                                                                                                                                                    
     ccGAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTaaa                                                                                                                                                <  2:690335/149‑1 (MQ=255)
       gAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAaata                                                                                                                                              <  2:1262048/149‑1 (MQ=255)
       gAATGGGAGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAaata                                                                                                                                              >  1:1148014/1‑149 (MQ=255)
          tgtgtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAtt                                                                                                                                           <  2:37753/146‑1 (MQ=255)
           ttgtgggtTACCAGTTCCCTGATTTTTTCTATCATTATAAAACCTATCTTATTATAAATCGATAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                          <  1:1249956/147‑1 (MQ=255)
           gggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                          >  1:131701/1‑149 (MQ=255)
           gggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                          <  2:1543613/149‑1 (MQ=255)
            ggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTg                                                                                                                                         <  2:560674/149‑1 (MQ=255)
             gtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGc                                                                                                                                        <  2:63388/149‑1 (MQ=255)
              tgggtTACTAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATATTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCt                                                                                                                                       <  1:807063/149‑1 (MQ=255)
              tgggtTACCAGTTCGCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTATATAATTTGCt                                                                                                                                       <  2:1241710/149‑1 (MQ=255)
              tgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCt                                                                                                                                       <  1:574289/149‑1 (MQ=255)
                  ttACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCt                                                                                                                                   <  2:1300332/149‑1 (MQ=255)
                  ttACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGTca                                                                                                                                   <  1:1281018/149‑2 (MQ=255)
                  ttACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACACCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGTca                                                                                                                                   <  2:327761/149‑2 (MQ=255)
                    aCCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTc                                                                                                                                 <  1:363731/149‑1 (MQ=255)
                    aCCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGTcaga                                                                                                                                 <  2:463747/149‑4 (MQ=255)
                     ccAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCa                                                                                                                                >  2:1501458/1‑149 (MQ=255)
                     ccAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAAACAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACATAAATTCTGACAGCAAAATATTTAATATGCTGTcagaa                                                                                                                                <  1:57745/149‑5 (MQ=255)
                      cAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCAc                                                                                                                               <  2:580972/149‑1 (MQ=255)
                           cccTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGATACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAAtt                                                                                                                          <  2:849786/149‑1 (MQ=255)
                           cccTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAAtt                                                                                                                          <  1:1493342/149‑1 (MQ=255)
                           cccTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAAtt                                                                                                                          <  2:1019070/149‑1 (MQ=255)
                              tGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGgt                                                                                                                       <  2:543415/149‑1 (MQ=255)
                                      tCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGATACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACtt                                                                                                               <  1:75843/149‑1 (MQ=255)
                                       cTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTa                                                                                                              >  1:625357/1‑149 (MQ=255)
                                          tCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCAATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCa                                                                                                           >  1:404754/1‑149 (MQ=255)
                                             tGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCAttt                                                                                                        >  1:1470043/1‑149 (MQ=255)
                                              gATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTc                                                                                                       <  2:944097/149‑1 (MQ=255)
                                              gATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACAAGAGACTATAATCACCACATTCGAAATATTCTGAGTGCTATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTTTTGTAAAATTACCATTTc                                                                                                       >  1:769865/1‑149 (MQ=255)
                                               aTAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTcc                                                                                                      <  1:3719/149‑1 (MQ=255)
                                                tAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACACCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCa                                                                                                     >  2:1288978/1‑149 (MQ=255)
                                                 aaaaCCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCaa                                                                                                    <  1:1136503/149‑1 (MQ=255)
                                                 aaaaCCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCaa                                                                                                    <  1:676618/149‑1 (MQ=255)
                                                   aaCCTATCTTATTATAAAACGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGa                                                                                                  <  2:1463070/149‑1 (MQ=255)
                                                      atATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACaa                                                                                               <  1:68537/148‑1 (MQ=255)
                                                              ttatAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTaa                                                                                       >  2:1061627/1‑149 (MQ=255)
                                                              ttatAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAAATTACCATTTCCAAGACAACTGACTaa                                                                                       >  1:181066/1‑149 (MQ=255)
                                                               tataAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAAc                                                                                      <  2:1147504/149‑1 (MQ=255)
                                                               tataAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAAc                                                                                      <  2:895074/149‑1 (MQ=255)
                                                                ataAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACt                                                                                     <  1:722882/149‑1 (MQ=255)
                                                                 taAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTc                                                                                    >  1:725802/1‑149 (MQ=255)
                                                                 taAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTc                                                                                    >  2:953748/1‑149 (MQ=255)
                                                                  aaaTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCa                                                                                   >  2:294388/1‑149 (MQ=255)
                                                                   aaTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGCCTAACTCAc                                                                                  >  2:732027/1‑149 (MQ=255)
                                                                   aaTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCAc                                                                                  >  2:1398103/1‑149 (MQ=255)
                                                                    aTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACt                                                                                 <  1:1077089/149‑1 (MQ=255)
                                                                       gagaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGa                                                                              >  2:120074/1‑149 (MQ=255)
                                                                       gagaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGa                                                                              >  2:245664/1‑149 (MQ=255)
                                                                        agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                             >  1:1533211/1‑149 (MQ=255)
                                                                        agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                             >  2:281191/1‑149 (MQ=255)
                                                                          aaaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAACTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGGGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATaa                                                                           <  2:1143697/149‑1 (MQ=255)
                                                                          aaaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATaa                                                                           <  1:1269501/149‑1 (MQ=255)
                                                                           aaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTATTTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAAt                                                                          <  1:1013927/149‑1 (MQ=255)
                                                                            aTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAGCTTTAATTCTGACAGCATATTAAATAATTTGGTGTCTTCACGAATTGGTGTAAAGTTACCATTTCCAAGACAACGGACTAACTCACTTGAGAAtt                                                                         >  2:135123/1‑149 (MQ=255)
                                                                            aTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAAtt                                                                         <  1:1392873/149‑1 (MQ=255)
                                                                             tCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAAtta                                                                        >  1:279328/1‑148 (MQ=255)
                                                                               ttGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGATGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCAt                                                                      <  2:212859/149‑1 (MQ=255)
                                                                                tGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCAtt                                                                     <  1:1224118/149‑1 (MQ=255)
                                                                                    aCCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATg                                                                 >  2:837953/1‑149 (MQ=255)
                                                                                     ccAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGa                                                                <  1:647299/149‑1 (MQ=255)
                                                                                     ccAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAAATTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTAATTTATGa                                                                >  1:266160/1‑149 (MQ=255)
                                                                                      cAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTTGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGAt                                                               <  1:465975/149‑1 (MQ=255)
                                                                                       agagACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGAtt                                                              <  1:503046/149‑1 (MQ=255)
                                                                                          taCTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTATGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGca                                                           <  1:156985/148‑1 (MQ=255)
                                                                                             tataATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcatca                                                        >  1:984886/1‑149 (MQ=255)
                                                                                              ataATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcatcat                                                       <  2:103513/149‑1 (MQ=255)
                                                                                               taATCACCCCATTCGAAATATTCTGAGTGCATTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGTTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCatcatc                                                      <  2:697596/149‑1 (MQ=255)
                                                                                               taATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCatcatc                                                      <  1:953748/149‑1 (MQ=255)
                                                                                                      cccATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACt                                               <  1:135900/149‑1 (MQ=255)
                                                                                                       ccATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTa                                              <  2:668400/149‑1 (MQ=255)
                                                                                                        aaTTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACTGCATATTAAATATTTTGCTGTCTTCACTAATTGTAGTAAACTTACCATTTCCAAGACAAGTGACTATCTGACTTGATAATTCATTTATGATTGCATCATCAGATACTaa                                             >  2:191675/2‑149 (MQ=255)
                                                                                                        aaTTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTGCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTaa                                             <  2:1525207/148‑1 (MQ=255)
                                                                                                         aTTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAAc                                            <  2:979604/149‑1 (MQ=255)
                                                                                                                  atTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCAtgttgt                                   <  2:821299/149‑1 (MQ=255)
                                                                                                                   ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                  <  2:206213/149‑1 (MQ=255)
                                                                                                                   ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                  <  1:492300/149‑1 (MQ=255)
                                                                                                                      tGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTa                               >  1:1458616/1‑149 (MQ=255)
                                                                                                                      tGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTa                               <  1:826498/149‑1 (MQ=255)
                                                                                                                       gAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTAt                              <  2:515105/149‑1 (MQ=255)
                                                                                                                             aaTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGa                        >  2:206945/1‑149 (MQ=255)
                                                                                                                             aaTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGa                        >  1:538103/1‑149 (MQ=255)
                                                                                                                              aTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGAt                       >  2:522822/1‑149 (MQ=255)
                                                                                                                               tAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAGCTCATGTTGTTTTATCGTAGAtt                      <  1:1350708/149‑1 (MQ=255)
                                                                                                                                  cTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAg                   >  2:1228081/1‑149 (MQ=255)
                                                                                                                                   ttAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGt                  >  1:281619/1‑149 (MQ=255)
                                                                                                                                   ttAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTAATGTTGTTTTTTAGTAGATTCAGt                  >  2:129797/1‑149 (MQ=255)
                                                                                                                                     aaaTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTAt                <  1:867243/149‑1 (MQ=255)
                                                                                                                                      aaTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTAtt               >  2:950408/1‑149 (MQ=255)
                                                                                                                                       aTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTa              <  2:404754/149‑1 (MQ=255)
                                                                                                                                          cTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAt           <  2:774059/149‑1 (MQ=255)
                                                                                                                                          cTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAt           <  1:1501458/149‑1 (MQ=255)
                                                                                                                                           tGACAGCATATTAAATAATTTGCTGTTTTCACAATTTGGTGTAAACTTACCATTTCCAAGACAACTTACTAACTCACTTGATAATTCATTTATGATTGCATCATCATATACTAACTCATTTTGTTTTATCGTAGATTCAGTTTTCGAtg          <  2:1415156/149‑1 (MQ=255)
                                                                                                                                            gACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAtgt         <  2:225448/149‑1 (MQ=255)
                                                                                                                                            gACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACGGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGTtgt         >  2:1517549/1‑149 (MQ=255)
                                                                                                                                             aCAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAtgtg        >  1:605874/1‑149 (MQ=255)
                                                                                                                                               aGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGAc      <  1:245664/149‑1 (MQ=255)
                                                                                                                                                tcATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACt     <  1:218034/148‑1 (MQ=255)
                                                                                                                                                 cATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTATATGTGACtt    >  1:347361/1‑149 (MQ=255)
                                                                                                                                                   tatTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACttta  <  1:1031108/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTATGCCGAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTA  >  NC_000913/2012006‑2012301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: