Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,012,158 A→T 6.0% pseudogene (194/312 nt) yedN ← pseudogene, IpaH/YopM family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,012,1580AT 6.0% 655.8 / 18.3 210pseudogene (194/312 nt)yedNpseudogene, IpaH/YopM family
Reads supporting (aligned to +/- strand):  ref base A (89/108);  new base T (7/6);  total (96/114)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

CCGAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTAA  >  NC_000913/2012011‑2012306
                                                                                                                                                   |                                                                                                                                                    
ccGAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTaaa                                                                                                                                                     <  2:690335/149‑1 (MQ=255)
      gggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                               >  1:131701/1‑149 (MQ=255)
      gggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                               <  2:1074644/149‑1 (MQ=255)
       ggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTg                                                                                                                                              >  1:225448/1‑149 (MQ=255)
       ggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTg                                                                                                                                              <  2:560674/149‑1 (MQ=255)
        gtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGc                                                                                                                                             <  2:63388/149‑1 (MQ=255)
        gtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTATTACAGCATATTAAATAATTTGc                                                                                                                                             >  1:123083/1‑149 (MQ=255)
         tgggtTACTAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATATTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCt                                                                                                                                            <  1:807063/149‑1 (MQ=255)
         tgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCt                                                                                                                                            <  1:574289/149‑1 (MQ=255)
           ggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGt                                                                                                                                          <  1:19022/149‑1 (MQ=255)
             ttACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCt                                                                                                                                        <  2:1300332/149‑1 (MQ=255)
             ttACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGTca                                                                                                                                        <  1:1281018/149‑2 (MQ=255)
              tACCAGTTCCCTTATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTTTAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTTACAGCTTATTAAATAATTTGCTGTCtt                                                                                                                                       <  2:1217880/149‑1 (MQ=255)
              tACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCtt                                                                                                                                       <  2:1050576/149‑1 (MQ=255)
                ccAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCa                                                                                                                                     >  2:1501458/1‑149 (MQ=255)
                ccAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAAACAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACATAAATTCTGACAGCAAAATATTTAATATGCTGTcagaa                                                                                                                                     <  1:57745/149‑5 (MQ=255)
                 cAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCAc                                                                                                                                    <  2:580972/149‑1 (MQ=255)
                 cAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCAc                                                                                                                                    <  2:1146757/149‑1 (MQ=255)
                      cccTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGATACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAAtt                                                                                                                               <  2:849786/149‑1 (MQ=255)
                      cccTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAAtt                                                                                                                               <  1:1493342/149‑1 (MQ=255)
                         tGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGgt                                                                                                                            <  2:543415/149‑1 (MQ=255)
                         tGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGgt                                                                                                                            >  2:826498/1‑149 (MQ=255)
                         tGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGgt                                                                                                                            <  1:1153757/149‑1 (MQ=255)
                             tttttCTATCATGATAAAACCTATCTTATTATAAAACGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTaa                                                                                                                        >  1:1463070/1‑149 (MQ=255)
                                 tCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGATACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACtt                                                                                                                    <  1:75843/149‑1 (MQ=255)
                                  cTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTa                                                                                                                   >  1:625357/1‑149 (MQ=255)
                                     tCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCa                                                                                                                >  1:519915/1‑149 (MQ=255)
                                       aTGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCAtt                                                                                                              >  1:487846/1‑149 (MQ=255)
                                        tGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCAttt                                                                                                             >  2:1026318/1‑149 (MQ=255)
                                         gATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTc                                                                                                            <  2:944097/149‑1 (MQ=255)
                                         gATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACAAGAGACTATAATCACCACATTCGAAATATTCTGAGTGCTATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTTTTGTAAAATTACCATTTc                                                                                                            >  1:769865/1‑149 (MQ=255)
                                          aTAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTcc                                                                                                           >  1:1415156/1‑149 (MQ=255)
                                           tAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACACCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCa                                                                                                          >  2:1288978/1‑149 (MQ=255)
                                            aaaaCCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCaa                                                                                                         <  1:413681/149‑1 (MQ=255)
                                            aaaaCCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCaa                                                                                                         <  1:1136503/149‑1 (MQ=255)
                                              aaCCTATCTTATTATAAAACGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGa                                                                                                       <  2:1463070/149‑1 (MQ=255)
                                                 atATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACaa                                                                                                    <  1:68537/148‑1 (MQ=255)
                                                  tATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAAc                                                                                                   >  1:936241/1‑149 (MQ=255)
                                                      ttattaTAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGAc                                                                                               >  2:348148/1‑149 (MQ=255)
                                                          tataAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAAc                                                                                           <  2:895074/149‑1 (MQ=255)
                                                           ataAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACt                                                                                          <  1:722882/149‑1 (MQ=255)
                                                            taAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTc                                                                                         >  2:953748/1‑149 (MQ=255)
                                                            taAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTc                                                                                         >  1:725802/1‑149 (MQ=255)
                                                             aaaTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCa                                                                                        >  2:294388/1‑149 (MQ=255)
                                                              aaTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGCCTAACTCAc                                                                                       >  2:732027/1‑149 (MQ=255)
                                                              aaTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCAc                                                                                       >  2:1398103/1‑149 (MQ=255)
                                                               aTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACt                                                                                      <  1:1077089/149‑1 (MQ=255)
                                                                  gagaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGa                                                                                   >  2:245664/1‑149 (MQ=255)
                                                                   agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                  >  2:281191/1‑149 (MQ=255)
                                                                   agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                  <  2:1530773/149‑1 (MQ=255)
                                                                   agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                  >  1:1533211/1‑149 (MQ=255)
                                                                    gaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATACTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTACAAGACAACTGACGAACTCACTTGATa                                                                                 >  1:288959/1‑149 (MQ=255)
                                                                     aaaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATaa                                                                                <  1:1269501/149‑1 (MQ=255)
                                                                      aaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTATTTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAAt                                                                               <  1:1013927/149‑1 (MQ=255)
                                                                       aTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAGCTTTAATTCTGACAGCATATTAAATAATTTGGTGTCTTCACGAATTGGTGTAAAGTTACCATTTCCAAGACAACGGACTAACTCACTTGAGAAtt                                                                              >  2:135123/1‑149 (MQ=255)
                                                                       aTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAAtt                                                                              <  1:1392873/149‑1 (MQ=255)
                                                                          ttGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGATGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCAt                                                                           <  2:212859/149‑1 (MQ=255)
                                                                             aaaaCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTa                                                                        <  2:1002096/149‑1 (MQ=255)
                                                                                ccAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGa                                                                     <  1:647299/149‑1 (MQ=255)
                                                                                     taCTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTATGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGca                                                                <  1:156985/148‑1 (MQ=255)
                                                                                        tataATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCATGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcatca                                                             >  2:454425/1‑149 (MQ=255)
                                                                                        tataATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcatca                                                             >  1:984886/1‑149 (MQ=255)
                                                                                         ataATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcatcat                                                            <  2:789428/149‑1 (MQ=255)
                                                                                          taATCACCCCATTCGAAATATTCTGAGTGCATTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGTTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCatcatc                                                           <  2:697596/149‑1 (MQ=255)
                                                                                                 cccATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACt                                                    >  2:492300/1‑149 (MQ=255)
                                                                                                 cccATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACt                                                    >  2:384817/1‑149 (MQ=255)
                                                                                                 cccATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACt                                                    <  1:135900/149‑1 (MQ=255)
                                                                                                  ccATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTa                                                   <  2:668400/149‑1 (MQ=255)
                                                                                                   cATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTaa                                                  >  2:1252197/1‑149 (MQ=255)
                                                                                                   cATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTaa                                                  <  1:348148/149‑1 (MQ=255)
                                                                                                   aaTTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACTGCATATTAAATATTTTGCTGTCTTCACTAATTGTAGTAAACTTACCATTTCCAAGACAAGTGACTATCTGACTTGATAATTCATTTATGATTGCATCATCAGATACTaa                                                  >  2:191675/2‑149 (MQ=255)
                                                                                                    aTTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAAc                                                 <  2:979604/149‑1 (MQ=255)
                                                                                                      tCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTc                                               <  1:281191/149‑1 (MQ=255)
                                                                                                              ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                       <  2:206213/149‑1 (MQ=255)
                                                                                                               tCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGttt                                      >  1:496906/1‑149 (MQ=255)
                                                                                                                      gCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTa                               <  1:670569/149‑1 (MQ=255)
                                                                                                                        aaTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGa                             >  2:206945/1‑149 (MQ=255)
                                                                                                                         aTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGAt                            >  2:522822/1‑149 (MQ=255)
                                                                                                                          tAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAGCTCATGTTGTTTTATCGTAGAtt                           <  1:1350708/149‑1 (MQ=255)
                                                                                                                              ttAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTAATGTTGTTTTTTAGTAGATTCAGt                       >  2:129797/1‑149 (MQ=255)
                                                                                                                                aaaTTCTGAAAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTTTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTAt                     <  1:268400/149‑1 (MQ=255)
                                                                                                                                 aaTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTAtt                    >  2:950408/1‑149 (MQ=255)
                                                                                                                                 aaTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTAtt                    <  1:369589/149‑1 (MQ=255)
                                                                                                                                  gtgaagACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTa                   >  1:833480/6‑149 (MQ=255)
                                                                                                                                   tgaagACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAg                  >  2:1182221/5‑149 (MQ=255)
                                                                                                                                     cTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAt                <  1:1501458/149‑1 (MQ=255)
                                                                                                                                     cTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAt                <  2:774059/149‑1 (MQ=255)
                                                                                                                                     aagACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAt                >  1:262128/3‑149 (MQ=255)
                                                                                                                                      tGACAGCATATTAAATAATTTGCTGTTTTCACAATTTGGTGTAAACTTACCATTTCCAAGACAACTTACTAACTCACTTGATAATTCATTTATGATTGCATCATCATATACTAACTCATTTTGTTTTATCGTAGATTCAGTTTTCGAtg               <  2:1415156/149‑1 (MQ=255)
                                                                                                                                       gACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAtgt              <  2:225448/149‑1 (MQ=255)
                                                                                                                                       gACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGCCAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTGGTTGTATCGTAGATTCAGTATTAGAtgg              >  2:1523627/1‑148 (MQ=255)
                                                                                                                                       gACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACGGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGTtgt              >  2:1517549/1‑149 (MQ=255)
                                                                                                                                        aCAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAtgtg             >  1:372917/1‑149 (MQ=255)
                                                                                                                                        aCAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAtgtg             >  1:605874/1‑149 (MQ=255)
                                                                                                                                            cATATTAAATAATTTGCTGTCTTCAATAATTGGTTTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCCTCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtt         <  2:123083/149‑1 (MQ=255)
                                                                                                                                              tatTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACttta       <  1:1031108/149‑1 (MQ=255)
                                                                                                                                               atTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttat      >  1:955619/1‑149 (MQ=255)
                                                                                                                                                ttAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttatt     >  1:1147433/1‑149 (MQ=255)
                                                                                                                                                ttAAATAAATTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttatt     <  2:131701/149‑1 (MQ=255)
                                                                                                                                                   aataatTTGCTGTCTTCACTAATTGGTGTAATCTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTaa  <  2:307195/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTAA  >  NC_000913/2012011‑2012306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: