Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,012,159 A→T 5.6% pseudogene (193/312 nt) yedN ← pseudogene, IpaH/YopM family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,012,1590AT 5.6% 667.6 / 17.1 210pseudogene (193/312 nt)yedNpseudogene, IpaH/YopM family
Reads supporting (aligned to +/- strand):  ref base A (89/109);  new base T (7/5);  total (96/114)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 222 total aligned reads displayed.

CCGAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTAAT  >  NC_000913/2012011‑2012307
                                                                                                                                                    |                                                                                                                                                    
  gAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAaata                                                                                                                                                    <  2:1262048/149‑1 (MQ=255)
  gAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAaata                                                                                                                                                    >  1:1530773/1‑149 (MQ=255)
     tgtgtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAtt                                                                                                                                                 <  2:37753/146‑1 (MQ=255)
     tgggtgggTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAtt                                                                                                                                                 <  2:740781/149‑1 (MQ=255)
      ttgtgggtTACCAGTTCCCTGATTTTTTCTATCATTATAAAACCTATCTTATTATAAATCGATAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                                <  1:1249956/147‑1 (MQ=255)
      gggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                                <  2:1074644/149‑1 (MQ=255)
      gggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                                >  1:131701/1‑149 (MQ=255)
         tgggtTACTAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATATTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCt                                                                                                                                             <  1:807063/149‑1 (MQ=255)
         tgggtTACCAGTTCGCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTATATAATTTGCt                                                                                                                                             <  2:1241710/149‑1 (MQ=255)
             ttACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCt                                                                                                                                         <  2:1300332/149‑1 (MQ=255)
             ttACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGTca                                                                                                                                         <  1:1281018/149‑2 (MQ=255)
              tACCAGTTCCCTTATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTTTAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTTACAGCTTATTAAATAATTTGCTGTCtt                                                                                                                                        <  2:1217880/149‑1 (MQ=255)
              tACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCtt                                                                                                                                        <  2:601004/149‑1 (MQ=255)
              tACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCtt                                                                                                                                        <  2:1050576/149‑1 (MQ=255)
               aCCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTc                                                                                                                                       <  1:363731/149‑1 (MQ=255)
               aCCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGTcaga                                                                                                                                       <  2:463747/149‑4 (MQ=255)
                ccAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCa                                                                                                                                      >  2:1501458/1‑149 (MQ=255)
                 cAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCAc                                                                                                                                     <  2:1146757/149‑1 (MQ=255)
                   gTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTa                                                                                                                                   >  1:678823/1‑149 (MQ=255)
                    ttCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGTGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTaa                                                                                                                                  <  2:1399754/149‑1 (MQ=255)
                      cccTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAAtt                                                                                                                                <  2:1387929/149‑1 (MQ=255)
                       cattaTTTTTTCTATAATGATAAAACCTATCTTATTATAAATCGAGAAATATTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTg                                                                                                                               <  1:1494439/145‑1 (MQ=255)
                        cTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACAAGAGACTATAATCACACCATTCGAAATATTATGAGTTCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTATTTgg                                                                                                                              >  1:62228/1‑149 (MQ=255)
                         tGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGgt                                                                                                                             >  2:826498/1‑149 (MQ=255)
                         tGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGgt                                                                                                                             <  1:571247/149‑1 (MQ=255)
                            ttttttCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTa                                                                                                                          >  2:722882/1‑149 (MQ=255)
                                 tCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGATACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACtt                                                                                                                     <  1:75843/149‑1 (MQ=255)
                                  cTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTa                                                                                                                    <  1:694229/149‑1 (MQ=255)
                                  atATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTa                                                                                                                    <  1:1368705/148‑1 (MQ=255)
                                    aTCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGAAAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTAcc                                                                                                                  >  2:240055/1‑149 (MQ=255)
                                        tGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCAttt                                                                                                              >  2:575339/1‑149 (MQ=255)
                                        tGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCAttt                                                                                                              >  2:1026318/1‑149 (MQ=255)
                                        tGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCAttt                                                                                                              >  1:1470043/1‑149 (MQ=255)
                                         gATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTc                                                                                                             <  2:944097/149‑1 (MQ=255)
                                         gATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACAAGAGACTATAATCACCACATTCGAAATATTCTGAGTGCTATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTTTTGTAAAATTACCATTTc                                                                                                             >  1:769865/1‑149 (MQ=255)
                                          aTAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTcc                                                                                                            <  1:3719/149‑1 (MQ=255)
                                          aTAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTcc                                                                                                            >  1:1415156/1‑149 (MQ=255)
                                           tAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACACCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCa                                                                                                           >  2:1288978/1‑149 (MQ=255)
                                            aaaaCCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCaa                                                                                                          <  1:1136503/149‑1 (MQ=255)
                                            aaaaCCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCaa                                                                                                          <  1:413681/149‑1 (MQ=255)
                                                            taAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTc                                                                                          >  2:953748/1‑149 (MQ=255)
                                                             aaaTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCa                                                                                         >  2:294388/1‑149 (MQ=255)
                                                               aTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACt                                                                                       <  1:1077089/149‑1 (MQ=255)
                                                                   agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                   <  2:861484/149‑1 (MQ=255)
                                                                   agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                   >  2:281191/1‑149 (MQ=255)
                                                                   agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                   <  2:1530773/149‑1 (MQ=255)
                                                                   agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAAAATAAATTCTGACAGCATATTAAATAATTTGCTTTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                   >  1:160319/1‑149 (MQ=255)
                                                                    gaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATACTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTACAAGACAACTGACGAACTCACTTGATa                                                                                  >  1:288959/1‑149 (MQ=255)
                                                                     aaaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATaa                                                                                 <  1:1269501/149‑1 (MQ=255)
                                                                      aaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTATTTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAAt                                                                                <  1:1013927/149‑1 (MQ=255)
                                                                         cTTGAAACCAGAGACTATAATTACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCa                                                                             >  1:1541618/1‑149 (MQ=255)
                                                                          ttGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGATGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCAt                                                                            <  2:212859/149‑1 (MQ=255)
                                                                           tGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCAtt                                                                           <  1:1224118/149‑1 (MQ=255)
                                                                             aaaaCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTa                                                                         <  2:1002096/149‑1 (MQ=255)
                                                                             aaaCCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTa                                                                         <  2:693567/149‑1 (MQ=255)
                                                                             aaaCCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTa                                                                         <  2:845972/149‑1 (MQ=255)
                                                                                 cAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTTGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGAt                                                                     <  1:465975/149‑1 (MQ=255)
                                                                                  agagACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGAtt                                                                    <  1:503046/149‑1 (MQ=255)
                                                                                        tataATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcatca                                                              >  1:984886/1‑149 (MQ=255)
                                                                                         ataATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcatcat                                                             <  2:789428/149‑1 (MQ=255)
                                                                                             tCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAAAAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGa                                                         <  1:633536/149‑1 (MQ=255)
                                                                                                   aaTTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACTGCATATTAAATATTTTGCTGTCTTCACTAATTGTAGTAAACTTACCATTTCCAAGACAAGTGACTATCTGACTTGATAATTCATTTATGATTGCATCATCAGATACTaa                                                   >  2:191675/2‑149 (MQ=255)
                                                                                                    aTTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAAc                                                  <  2:979604/149‑1 (MQ=255)
                                                                                                      tCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTc                                                <  1:281191/149‑1 (MQ=255)
                                                                                                      tCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTc                                                >  1:723013/1‑149 (MQ=255)
                                                                                                        taaaTATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCAt                                              <  2:552227/148‑1 (MQ=255)
                                                                                                          aaTATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCAtgt                                            <  1:575339/149‑1 (MQ=255)
                                                                                                              ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                        <  1:492300/149‑1 (MQ=255)
                                                                                                              ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                        <  2:683206/149‑1 (MQ=255)
                                                                                                              ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                        <  2:206213/149‑1 (MQ=255)
                                                                                                              ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                        >  2:248736/1‑149 (MQ=255)
                                                                                                                 tGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTa                                     <  1:826498/149‑1 (MQ=255)
                                                                                                                  gAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTAt                                    <  2:515105/149‑1 (MQ=255)
                                                                                                                      gCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTa                                <  1:670569/149‑1 (MQ=255)
                                                                                                                        aaTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGa                              >  2:206945/1‑149 (MQ=255)
                                                                                                                              ttAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTTTTTTATCGTAGATTCAGt                        >  2:656270/1‑149 (MQ=255)
                                                                                                                              ttAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGt                        >  2:532015/1‑149 (MQ=255)
                                                                                                                              ttAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTAATGTTGTTTTTTAGTAGATTCAGt                        >  2:129797/1‑149 (MQ=255)
                                                                                                                                aaaTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTAt                      <  1:867243/149‑1 (MQ=255)
                                                                                                                                aaaTTCTGAAAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTTTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTAt                      <  1:268400/149‑1 (MQ=255)
                                                                                                                                 aaTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTAtt                     >  2:950408/1‑149 (MQ=255)
                                                                                                                                  aTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTa                    <  2:404754/149‑1 (MQ=255)
                                                                                                                                   tgaagACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAg                   >  2:1182221/5‑149 (MQ=255)
                                                                                                                                    tCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTATACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGa                  >  1:58219/1‑149 (MQ=255)
                                                                                                                                     cTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAt                 <  2:774059/149‑1 (MQ=255)
                                                                                                                                     aagACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAt                 >  1:262128/3‑149 (MQ=255)
                                                                                                                                      tGACAGCATATTAAATAATTTGCTGTTTTCACAATTTGGTGTAAACTTACCATTTCCAAGACAACTTACTAACTCACTTGATAATTCATTTATGATTGCATCATCATATACTAACTCATTTTGTTTTATCGTAGATTCAGTTTTCGAtg                <  2:1415156/149‑1 (MQ=255)
                                                                                                                                      agACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAtg                >  1:1354374/2‑149 (MQ=255)
                                                                                                                                       gACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGCCAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTGGTTGTATCGTAGATTCAGTATTAGAtgg               >  2:1523627/1‑148 (MQ=255)
                                                                                                                                       gACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACGGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGTtgt               >  2:1517549/1‑149 (MQ=255)
                                                                                                                                          aGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGAc            <  1:245664/149‑1 (MQ=255)
                                                                                                                                           tcATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACt           <  1:218034/148‑1 (MQ=255)
                                                                                                                                            cATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTATATGTGACtt          >  1:347361/1‑149 (MQ=255)
                                                                                                                                              tatTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACttta        <  1:1031108/149‑1 (MQ=255)
                                                                                                                                               atTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttat       <  1:1398103/149‑1 (MQ=255)
                                                                                                                                               atTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttat       >  1:955619/1‑149 (MQ=255)
                                                                                                                                                ttAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttatt      >  1:1147433/1‑149 (MQ=255)
                                                                                                                                                 tAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttattt     <  2:1328376/149‑1 (MQ=255)
                                                                                                                                                   aataatTTGCTGTCTTCACTAATTGGTGTAATCTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTaa   <  2:307195/149‑1 (MQ=255)
                                                                                                                                                    ataatTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTAAt  <  2:1470043/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGAATGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTAAT  >  NC_000913/2012011‑2012307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: