Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,012,164 T→A 6.1% pseudogene (188/312 nt) yedN ← pseudogene, IpaH/YopM family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,012,1640TA 6.1% 641.7 / 22.8 215pseudogene (188/312 nt)yedNpseudogene, IpaH/YopM family
Reads supporting (aligned to +/- strand):  ref base T (91/111);  new base A (7/6);  total (98/117)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-01

Only 100 of 223 total aligned reads displayed.

TGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTAATTGAAC  >  NC_000913/2012016‑2012312
                                                                                                                                                    |                                                                                                                                                    
tgggtgggTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAtt                                                                                                                                                      <  2:740781/149‑1 (MQ=255)
 ttgtgggtTACCAGTTCCCTGATTTTTTCTATCATTATAAAACCTATCTTATTATAAATCGATAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                                     <  1:1249956/147‑1 (MQ=255)
 gggtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAAttt                                                                                                                                                     <  2:1074644/149‑1 (MQ=255)
   gtgggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGc                                                                                                                                                   <  2:63388/149‑1 (MQ=255)
    tgggtTACTAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATATTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCt                                                                                                                                                  <  1:807063/149‑1 (MQ=255)
    tgggtTACCAGTTCGCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTATATAATTTGCt                                                                                                                                                  <  2:1241710/149‑1 (MQ=255)
      ggtTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGt                                                                                                                                                <  1:19022/149‑1 (MQ=255)
        ttACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCt                                                                                                                                              <  2:1300332/149‑1 (MQ=255)
        ttACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGTca                                                                                                                                              <  1:1281018/149‑2 (MQ=255)
         tACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCtt                                                                                                                                             <  2:601004/149‑1 (MQ=255)
         tACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCtt                                                                                                                                             <  2:1050576/149‑1 (MQ=255)
          aCCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTc                                                                                                                                            <  1:363731/149‑1 (MQ=255)
          aCCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCAAATTATTTAATATGCTGTcaga                                                                                                                                            <  2:463747/149‑4 (MQ=255)
              gTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTa                                                                                                                                        >  1:678823/1‑149 (MQ=255)
               ttCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGTGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTaa                                                                                                                                       <  2:1399754/149‑1 (MQ=255)
                 cccTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAAtt                                                                                                                                     <  2:1019070/149‑1 (MQ=255)
                 cccTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAAtt                                                                                                                                     <  2:1387929/149‑1 (MQ=255)
                  cattaTTTTTTCTATAATGATAAAACCTATCTTATTATAAATCGAGAAATATTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTg                                                                                                                                    <  1:1494439/145‑1 (MQ=255)
                    tGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGgt                                                                                                                                  <  1:571247/149‑1 (MQ=255)
                    tGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGgt                                                                                                                                  >  2:826498/1‑149 (MQ=255)
                       ttttttCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTa                                                                                                                               >  2:722882/1‑149 (MQ=255)
                            tCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGATACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACtt                                                                                                                          <  1:75843/149‑1 (MQ=255)
                             cTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTa                                                                                                                         <  1:694229/149‑1 (MQ=255)
                             cTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTa                                                                                                                         >  1:625357/1‑149 (MQ=255)
                             atATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTa                                                                                                                         <  1:1368705/148‑1 (MQ=255)
                              tATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTAc                                                                                                                        >  1:1143046/1‑149 (MQ=255)
                                tCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCa                                                                                                                      >  1:519915/1‑149 (MQ=255)
                                   tGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCAttt                                                                                                                   >  2:575339/1‑149 (MQ=255)
                                   tGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCAttt                                                                                                                   >  1:1470043/1‑149 (MQ=255)
                                    gATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTc                                                                                                                  <  2:944097/149‑1 (MQ=255)
                                    gATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACAAGAGACTATAATCACCACATTCGAAATATTCTGAGTGCTATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTTTTGTAAAATTACCATTTc                                                                                                                  >  1:769865/1‑149 (MQ=255)
                                     aTAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTcc                                                                                                                 >  1:1415156/1‑149 (MQ=255)
                                      tAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACACCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCa                                                                                                                >  2:1288978/1‑149 (MQ=255)
                                       aaaaCCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCaa                                                                                                               <  1:413681/149‑1 (MQ=255)
                                         aaCCTATCTTATTATAAAACGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGa                                                                                                             <  2:1463070/149‑1 (MQ=255)
                                             tATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAAc                                                                                                         >  1:936241/1‑149 (MQ=255)
                                              aTCTTATTATATATCGAGAAATCTTGAAACCAGAGACTATAATCAACCCATTAGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTATTCACTAATTGGTGTAAACTTAACATTTCCAAGACAACt                                                                                                        <  1:1346312/149‑1 (MQ=255)
                                                 ttattaTAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGAc                                                                                                     >  2:348148/1‑149 (MQ=255)
                                                       taAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTc                                                                                               >  1:725802/1‑149 (MQ=255)
                                                       taAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTc                                                                                               >  2:953748/1‑149 (MQ=255)
                                                        aaaTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCa                                                                                              >  2:294388/1‑149 (MQ=255)
                                                          aTCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACt                                                                                            <  1:1077089/149‑1 (MQ=255)
                                                             gagaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGa                                                                                         >  2:120074/1‑149 (MQ=255)
                                                              agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                        >  2:281191/1‑149 (MQ=255)
                                                              agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                        <  2:1530773/149‑1 (MQ=255)
                                                              agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                        >  1:1533211/1‑149 (MQ=255)
                                                              agaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGAt                                                                                        <  2:861484/149‑1 (MQ=255)
                                                               gaAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATACTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTACAAGACAACTGACGAACTCACTTGATa                                                                                       >  1:288959/1‑149 (MQ=255)
                                                                aaaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAACTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGGGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATaa                                                                                      <  2:1143697/149‑1 (MQ=255)
                                                                aaaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATaa                                                                                      <  1:1269501/149‑1 (MQ=255)
                                                                 aaTCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTATTTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAAt                                                                                     <  1:1013927/149‑1 (MQ=255)
                                                                   tCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAAtta                                                                                   >  1:279328/1‑148 (MQ=255)
                                                                     ttGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGATGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCAt                                                                                 <  2:212859/149‑1 (MQ=255)
                                                                      tGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCAtt                                                                                <  1:1224118/149‑1 (MQ=255)
                                                                        aaaCCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTa                                                                              <  2:845972/149‑1 (MQ=255)
                                                                        aaaCCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTa                                                                              <  2:693567/149‑1 (MQ=255)
                                                                         aaCCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTAt                                                                             <  2:183114/149‑1 (MQ=255)
                                                                           ccAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGa                                                                           <  1:647299/149‑1 (MQ=255)
                                                                            cAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTTGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGAt                                                                          <  1:465975/149‑1 (MQ=255)
                                                                                taCTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTATGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGca                                                                      <  1:156985/148‑1 (MQ=255)
                                                                                 aCTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcat                                                                     >  2:670569/1‑149 (MQ=255)
                                                                                   tataATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcatca                                                                   >  1:984886/1‑149 (MQ=255)
                                                                                    ataATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGcatcat                                                                  <  2:789428/149‑1 (MQ=255)
                                                                                            cccATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACt                                                          <  2:1472758/149‑1 (MQ=255)
                                                                                               aTTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAAc                                                       <  2:979604/149‑1 (MQ=255)
                                                                                                 tCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTc                                                     <  1:281191/149‑1 (MQ=255)
                                                                                                   taaaTATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCAt                                                   <  2:552227/148‑1 (MQ=255)
                                                                                                   gAAATATTCTGAGTGCAATAACTTAAATTCTGAAAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTTTAAAATTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATAATCAGATACTAACTCAt                                                   >  2:1027518/1‑149 (MQ=255)
                                                                                                     aaTATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCAtgt                                                 <  1:575339/149‑1 (MQ=255)
                                                                                                         ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                             <  1:492300/149‑1 (MQ=255)
                                                                                                         ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                             <  2:206213/149‑1 (MQ=255)
                                                                                                         ttCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATgttgtt                                             >  2:248736/1‑149 (MQ=255)
                                                                                                             gAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTAt                                         <  2:515105/149‑1 (MQ=255)
                                                                                                                 gCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTa                                     <  1:670569/149‑1 (MQ=255)
                                                                                                                   aaTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGa                                   >  2:206945/1‑149 (MQ=255)
                                                                                                                    aTAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGAt                                  >  2:522822/1‑149 (MQ=255)
                                                                                                                        cTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAg                              >  2:1228081/1‑149 (MQ=255)
                                                                                                                         ttAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTAATGTTGTTTTTTAGTAGATTCAGt                             >  2:129797/1‑149 (MQ=255)
                                                                                                                            aaTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTAtt                          >  2:950408/1‑149 (MQ=255)
                                                                                                                             gtgaagACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTa                         >  1:833480/6‑149 (MQ=255)
                                                                                                                             gtgaagACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTa                         >  1:1125334/6‑149 (MQ=255)
                                                                                                                             aTTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTa                         <  2:404754/149‑1 (MQ=255)
                                                                                                                               tCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTATACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGa                       >  1:58219/1‑149 (MQ=255)
                                                                                                                                aagACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAt                      >  1:262128/3‑149 (MQ=255)
                                                                                                                                 tGACAGCATATTAAATAATTTGCTGTTTTCACAATTTGGTGTAAACTTACCATTTCCAAGACAACTTACTAACTCACTTGATAATTCATTTATGATTGCATCATCATATACTAACTCATTTTGTTTTATCGTAGATTCAGTTTTCGAtg                     <  2:1415156/149‑1 (MQ=255)
                                                                                                                                 agACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAtg                     >  1:1354374/2‑149 (MQ=255)
                                                                                                                                  gACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAtgt                    <  2:225448/149‑1 (MQ=255)
                                                                                                                                  gACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGCCAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTGGTTGTATCGTAGATTCAGTATTAGAtgg                    >  2:1523627/1‑148 (MQ=255)
                                                                                                                                  gACAGCAAATTATTTAATATGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACGGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGTtgt                    >  2:1517549/1‑149 (MQ=255)
                                                                                                                                   aCAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGAtgtg                   >  1:372917/1‑149 (MQ=255)
                                                                                                                                     aGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGAc                 <  1:245664/149‑1 (MQ=255)
                                                                                                                                      tcATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACt                <  1:218034/148‑1 (MQ=255)
                                                                                                                                       cATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTATATGTGACtt               >  1:347361/1‑149 (MQ=255)
                                                                                                                                         tatTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACttta             <  1:1031108/149‑1 (MQ=255)
                                                                                                                                          atTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttat            >  1:955619/1‑149 (MQ=255)
                                                                                                                                          atTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttat            <  1:1398103/149‑1 (MQ=255)
                                                                                                                                           ttAAATAAATTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACtttatt           <  2:131701/149‑1 (MQ=255)
                                                                                                                                              aataatTTGCTGTCTTCACTAATTGGTGTAATCTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTaa        <  2:307195/149‑1 (MQ=255)
                                                                                                                                               ataatTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTAAt       <  2:769865/149‑1 (MQ=255)
                                                                                                                                                taatTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTAAtt      <  2:1148014/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGGTGGGTTACCAGTTCCCTGATTTTTTCTATCATGATAAAACCTATCTTATTATAAATCGAGAAATCTTGAAACCAGAGACTATAATCACCCCATTCGAAATATTCTGAGTGCAATAACTTAAATTCTGACAGCATATTAAATAATTTGCTGTCTTCACTAATTGGTGTAAACTTACCATTTCCAAGACAACTGACTAACTCACTTGATAATTCATTTATGATTGCATCATCAGATACTAACTCATGTTGTTTTATCGTAGATTCAGTATTAGATGTGACTTTATTTAATTGAAC  >  NC_000913/2012016‑2012312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: