Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,028,799 A→G 6.9% Y190H (TAC→CAC)  yedI ← DUF808 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,7990AG 6.9% 307.0 / 9.8 102Y190H (TAC→CAC) yedIDUF808 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (52/42);  new base G (4/3);  total (57/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

Only 100 of 104 total aligned reads displayed.

GCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCTTT  >  NC_000913/2028651‑2028947
                                                                                                                                                    |                                                                                                                                                    
gCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTa                                                                                                                                                      <  1:1208314/149‑1 (MQ=255)
       tGCCGAAAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGccc                                                                                                                                               <  2:1406424/149‑1 (MQ=255)
            aCAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGt                                                                                                                                          <  2:54408/149‑1 (MQ=255)
            aCAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGt                                                                                                                                          >  2:1298682/1‑149 (MQ=255)
             cAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTc                                                                                                                                         >  1:569579/1‑149 (MQ=255)
               aTCGATAACGCTTTCATCAGCCCGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTGGGCGTCTACGGTctg                                                                                                                                       <  1:117100/149‑3 (MQ=255)
               aTCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTGGGCGTCTACGGTctg                                                                                                                                       <  1:896558/149‑3 (MQ=255)
                  gATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAGTTTTCCGCCAGCCAATACACCTGGTCATCAATCTTACCGATACCCCCTACCAGACCGTAGACGCCCCCGGTCACTAc                                                                                                                                    >  1:60931/1‑149 (MQ=255)
                   aTAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTAcc                                                                                                                                   >  2:894747/1‑149 (MQ=255)
                   aTAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTAcc                                                                                                                                   >  1:1207983/1‑149 (MQ=255)
                    tatcGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCATCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCa                                                                                                                                  <  2:697612/146‑1 (MQ=255)
                    tAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCa                                                                                                                                  >  2:355689/1‑149 (MQ=255)
                        gCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAgcgc                                                                                                                              <  2:1592416/149‑1 (MQ=255)
                        gCTTTCATAAGCCAGGGCGCGATATTCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCTTCAATCTTAAAGATAACACCTACCAGACCGTAGACGCCCACGGTCACTAGCAgcgc                                                                                                                              <  2:1313388/149‑1 (MQ=255)
                           ttCATCAGCCAGGGCGCGATAATCAATCACCCTTAACCTAATGCCTGCATCAGCGCGCTGGATGTTTCCGCCAGCCAATACCCCAGGTCATCAAGCTTACCGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGAt                                                                                                                           <  1:837641/149‑1 (MQ=255)
                           agtcgtggCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGAt                                                                                                                           <  2:718482/142‑1 (MQ=255)
                            tcatcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATg                                                                                                                          >  2:531512/1‑149 (MQ=255)
                              atcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCCCGGTCACTACCAGCGCGATGcc                                                                                                                        >  2:1546610/1‑149 (MQ=255)
                              atcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCACTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGcc                                                                                                                        >  2:920889/1‑149 (MQ=255)
                               tcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCt                                                                                                                       >  1:1222443/1‑149 (MQ=255)
                               tcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTACGCCAGCCAATACCCCCGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGCCGCCCACGGTCACTACCAGCGCGATGCCt                                                                                                                       >  2:1012289/1‑149 (MQ=255)
                                caGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATGTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTg                                                                                                                      >  2:163967/1‑149 (MQ=255)
                                caGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTg                                                                                                                      >  1:1550238/1‑149 (MQ=255)
                                 aGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGa                                                                                                                     >  1:625662/1‑149 (MQ=255)
                                   ccAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGaaa                                                                                                                   <  2:1414774/149‑1 (MQ=255)
                                   ccAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGaaa                                                                                                                   <  1:573327/149‑1 (MQ=255)
                                    cAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAag                                                                                                                  <  2:569579/149‑1 (MQ=255)
                                       ggCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcac                                                                                                               <  2:384878/149‑1 (MQ=255)
                                        gcgcGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcacc                                                                                                              >  2:375394/1‑149 (MQ=255)
                                          gcgATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcaccag                                                                                                            <  1:765102/149‑1 (MQ=255)
                                            gATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcaccagca                                                                                                          >  2:384842/1‑149 (MQ=255)
                                                 tCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGa                                                                                                     <  2:559698/149‑1 (MQ=255)
                                                   aaTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTGAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACGACCAGCGCGATGCCTGAAAGCACCAGCACCTGAtt                                                                                                   >  2:305718/1‑149 (MQ=255)
                                                    aTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTa                                                                                                  >  2:558080/1‑149 (MQ=255)
                                                    aTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTa                                                                                                  >  2:435563/1‑149 (MQ=255)
                                                     tAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTaa                                                                                                 <  2:418708/149‑1 (MQ=255)
                                                     tAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTaa                                                                                                 <  1:1063344/149‑1 (MQ=255)
                                                       aCCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGc                                                                                               <  1:1563966/149‑1 (MQ=255)
                                                          cTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAAc                                                                                            >  1:1124261/1‑149 (MQ=255)
                                                          cTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAAc                                                                                            >  2:1428913/1‑149 (MQ=255)
                                                           tttACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACAAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACg                                                                                           <  1:1212933/149‑1 (MQ=255)
                                                            ttACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACgg                                                                                          <  1:1298682/149‑1 (MQ=255)
                                                             tACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGgc                                                                                         >  2:784428/1‑149 (MQ=255)
                                                              aCCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAGCGATAACCCCTACCAGACCGTCGACGCCCACGGTCACTACCAGCGCGATTCCTGAAAGCACCGGCACCTGAGTAAGCCACGgcc                                                                                        >  1:265393/1‑148 (MQ=255)
                                                              aCCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcacc                                                                                                              <  1:743984/127‑1 (MQ=255)
                                                              aCCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcacc                                                                                                              >  2:743984/1‑127 (MQ=255)
                                                              aCCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGgcg                                                                                        >  1:177356/1‑149 (MQ=255)
                                                                cTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCACCGGCGCt                                                                                      >  2:1327216/1‑149 (MQ=255)
                                                                  aaTGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTc                                                                                    >  2:1043983/1‑149 (MQ=255)
                                                                  aaTGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTc                                                                                    >  2:1204567/1‑149 (MQ=255)
                                                                    tGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCgg                                                                                  <  1:1511761/149‑1 (MQ=255)
                                                                     gCCTGCATCAGCGCGCTGGATTTTTCCGCGAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGc                                                                                 <  1:223114/149‑1 (MQ=255)
                                                                     gCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATAACCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGc                                                                                 <  1:105802/149‑1 (MQ=255)
                                                                         gCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCcaca                                                                             >  2:1575429/1‑149 (MQ=255)
                                                                         gCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCcaca                                                                             >  2:1527038/1‑149 (MQ=255)
                                                                            tCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATc                                                                          <  2:1222443/149‑1 (MQ=255)
                                                                              aGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATccc                                                                        >  2:204214/1‑149 (MQ=255)
                                                                              aGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATccc                                                                        <  1:355689/149‑1 (MQ=255)
                                                                                   gcTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCg                                                                   >  2:531720/1‑149 (MQ=255)
                                                                                    cTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGt                                                                  <  2:1207983/149‑1 (MQ=255)
                                                                                     tGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATACCCCCTACCAGACCGTAGACGCCCACGGTCCCTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCACCGGCGCTTCGGCCACAATCCACcgcggg                                                                 >  2:37912/1‑147 (MQ=255)
                                                                                        aTTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATg                                                              >  1:476391/1‑149 (MQ=255)
                                                                                        aTTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATg                                                              >  1:746738/1‑149 (MQ=255)
                                                                                         tttttCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATgg                                                             >  1:377474/1‑149 (MQ=255)
                                                                                         tttttCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATgg                                                             <  1:784428/149‑1 (MQ=255)
                                                                                                gccagccaATTCCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATCCCTGAAAGCCCCAGCACCTGAGTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGGGACCAt                                                      >  1:329002/1‑149 (MQ=255)
                                                                                                 ccagccaATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGAtt                                                     <  1:1546610/149‑1 (MQ=255)
                                                                                                       aaTACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCa                                               <  1:531512/149‑1 (MQ=255)
                                                                                                        aTACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCATCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAg                                              <  2:505043/149‑1 (MQ=255)
                                                                                                            cccAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAACGCCCCAGCACCTGATTAAGCAACGGCGCTGCGGCCACAATCCCCAGCGTGCTGGCGCCGAGTTCCGCAGACaa                                          >  1:49534/1‑149 (MQ=255)
                                                                                                            cccAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACaa                                          <  1:894747/149‑1 (MQ=255)
                                                                                                            cccAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACaa                                          >  2:1328867/1‑149 (MQ=255)
                                                                                                                    atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAAt                                  <  1:753830/149‑1 (MQ=255)
                                                                                                                    atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAAt                                  >  1:169197/1‑149 (MQ=255)
                                                                                                                    atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAAt                                  >  1:744975/1‑149 (MQ=255)
                                                                                                                    atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTAAATACCCGCGCGATGACAGCAAGAACCAGAACCTGATTACGACACGGCGCTTCTGACACAATACACAGCGAGATGTCGACGATTTCCGAAGAAAAAATaaaaa                                  >  2:213522/1‑148 (MQ=255)
                                                                                                                    atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACCGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGGAGACAATATAAAAt                                  <  1:236327/149‑1 (MQ=255)
                                                                                                                     tcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATc                                 <  1:435563/149‑1 (MQ=255)
                                                                                                                      caATCTTAACGATAACCCCTACCAGACCGTAGACGCACACGGTCACTACCAGCGCGATGCCTGAAAGCACCCGCGCCAGATTAAGCAACGGCGCTTCGGCCACAATCCCCCGCCTGATGGCGACGATTTCCGCAGACAATATAAAAACg                                >  1:641359/1‑149 (MQ=255)
                                                                                                                        aTCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGt                              >  1:1092689/1‑149 (MQ=255)
                                                                                                                          cTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTAc                            <  1:375394/149‑1 (MQ=255)
                                                                                                                          cTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTAc                            <  1:1428913/149‑1 (MQ=255)
                                                                                                                              aCGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAAt                        >  1:1243643/1‑149 (MQ=255)
                                                                                                                              aCGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAAt                        >  1:691006/1‑149 (MQ=255)
                                                                                                                                 aTAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGAGATGGCGACGATTTCCGCAGACAATATATAATCGGTACGAATCGc                     >  1:31547/1‑149 (MQ=255)
                                                                                                                                      cccTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCtt                >  1:1507707/1‑149 (MQ=255)
                                                                                                                                        cTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCtttt              >  1:611371/1‑149 (MQ=255)
                                                                                                                                          tagtgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGTCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAt            <  2:244301/145‑1 (MQ=255)
                                                                                                                                          tagtgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAt            >  1:1247690/5‑149 (MQ=255)
                                                                                                                                           ccAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCATTTTAtt           >  1:691103/1‑149 (MQ=255)
                                                                                                                                             tgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAtttt         >  2:654281/2‑149 (MQ=255)
                                                                                                                                             tgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAtttt         >  1:865497/2‑149 (MQ=255)
                                                                                                                                             tgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAtttt         >  1:578528/2‑149 (MQ=255)
                                                                                                                                              gACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCTGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTg        >  2:675319/1‑149 (MQ=255)
                                                                                                                                              gACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTg        >  2:213960/1‑149 (MQ=255)
                                                                                                                                                ccGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTc      <  2:476391/149‑1 (MQ=255)
                                                                                                                                                ccGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTc      <  2:625662/149‑1 (MQ=255)
                                                                                                                                                ccGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTc      <  2:746738/149‑1 (MQ=255)
                                                                                                                                                   tAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCtt   <  1:746772/149‑1 (MQ=255)
                                                                                                                                                    aGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCttt  <  1:384842/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCAGCGTGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCTTT  >  NC_000913/2028651‑2028947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: