Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,028,806 C→T 6.8% V187V (GTG→GTA yedI ← DUF808 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,8060CT 6.8% 317.5 / 8.9 102V187V (GTG→GTAyedIDUF808 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (53/42);  new base T (4/3);  total (57/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

Only 100 of 103 total aligned reads displayed.

TGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCTTT  >  NC_000913/2028658‑2028947
                                                                                                                                                    |                                                                                                                                             
tGCCGAAAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGccc                                                                                                                                               <  2:1406424/149‑1 (MQ=255)
     aCAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGt                                                                                                                                          >  2:1298682/1‑149 (MQ=255)
     aCAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGt                                                                                                                                          <  2:54408/149‑1 (MQ=255)
      cAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTc                                                                                                                                         >  1:569579/1‑149 (MQ=255)
        aTCGATAACGCTTTCATCAGCCCGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTGGGCGTCTACGGTctg                                                                                                                                       <  1:117100/149‑3 (MQ=255)
        aTCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTGGGCGTCTACGGTctg                                                                                                                                       <  1:896558/149‑3 (MQ=255)
           gATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGAGTTTTCCGCCAGCCAATACACCTGGTCATCAATCTTACCGATACCCCCTACCAGACCGTAGACGCCCCCGGTCACTAc                                                                                                                                    >  1:60931/1‑149 (MQ=255)
            aTAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTAcc                                                                                                                                   >  1:1207983/1‑149 (MQ=255)
            aTAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTAcc                                                                                                                                   >  2:894747/1‑149 (MQ=255)
             tatcGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCATCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCa                                                                                                                                  <  2:697612/146‑1 (MQ=255)
             tAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCa                                                                                                                                  >  2:355689/1‑149 (MQ=255)
                 gCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAgcgc                                                                                                                              <  2:1592416/149‑1 (MQ=255)
                 gCTTTCATAAGCCAGGGCGCGATATTCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCTTCAATCTTAAAGATAACACCTACCAGACCGTAGACGCCCACGGTCACTAGCAgcgc                                                                                                                              <  2:1313388/149‑1 (MQ=255)
                    ttCATCAGCCAGGGCGCGATAATCAATCACCCTTAACCTAATGCCTGCATCAGCGCGCTGGATGTTTCCGCCAGCCAATACCCCAGGTCATCAAGCTTACCGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGAt                                                                                                                           <  1:837641/149‑1 (MQ=255)
                    ttCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGAt                                                                                                                           <  1:853801/149‑1 (MQ=255)
                    agtcgtggCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGAt                                                                                                                           <  2:718482/142‑1 (MQ=255)
                     tcatcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATg                                                                                                                          >  2:531512/1‑149 (MQ=255)
                       atcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCCCGGTCACTACCAGCGCGATGcc                                                                                                                        >  2:1546610/1‑149 (MQ=255)
                       atcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCACTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGcc                                                                                                                        >  2:920889/1‑149 (MQ=255)
                        tcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCt                                                                                                                       >  1:1222443/1‑149 (MQ=255)
                        tcaGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTACGCCAGCCAATACCCCCGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGCCGCCCACGGTCACTACCAGCGCGATGCCt                                                                                                                       >  2:1012289/1‑149 (MQ=255)
                         caGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATGTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTg                                                                                                                      >  2:163967/1‑149 (MQ=255)
                         caGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTg                                                                                                                      >  1:1550238/1‑149 (MQ=255)
                          aGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGa                                                                                                                     >  1:625662/1‑149 (MQ=255)
                            ccAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGaaa                                                                                                                   <  2:1414774/149‑1 (MQ=255)
                             cAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAag                                                                                                                  <  2:569579/149‑1 (MQ=255)
                                ggCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcac                                                                                                               <  2:384878/149‑1 (MQ=255)
                                 gcgcGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcacc                                                                                                              >  2:375394/1‑149 (MQ=255)
                                   gcgATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcaccag                                                                                                            <  1:765102/149‑1 (MQ=255)
                                     gATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcaccagca                                                                                                          >  2:384842/1‑149 (MQ=255)
                                          tCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGa                                                                                                     >  2:345462/1‑149 (MQ=255)
                                          tCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGa                                                                                                     <  2:559698/149‑1 (MQ=255)
                                            aaTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTGAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACGACCAGCGCGATGCCTGAAAGCACCAGCACCTGAtt                                                                                                   >  2:305718/1‑149 (MQ=255)
                                             aTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTa                                                                                                  >  2:435563/1‑149 (MQ=255)
                                             aTAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTa                                                                                                  >  2:558080/1‑149 (MQ=255)
                                              tAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTaa                                                                                                 <  2:418708/149‑1 (MQ=255)
                                              tAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTaa                                                                                                 <  1:1063344/149‑1 (MQ=255)
                                              tAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTaa                                                                                                 <  1:345462/149‑1 (MQ=255)
                                                aCCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGc                                                                                               <  1:1563966/149‑1 (MQ=255)
                                                   cTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAAc                                                                                            >  2:1428913/1‑149 (MQ=255)
                                                   cTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAAc                                                                                            >  1:1124261/1‑149 (MQ=255)
                                                    tttACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACAAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACg                                                                                           <  1:1212933/149‑1 (MQ=255)
                                                     ttACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACgg                                                                                          <  1:1298682/149‑1 (MQ=255)
                                                      tACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGgc                                                                                         >  2:784428/1‑149 (MQ=255)
                                                       aCCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAGCGATAACCCCTACCAGACCGTCGACGCCCACGGTCACTACCAGCGCGATTCCTGAAAGCACCGGCACCTGAGTAAGCCACGgcc                                                                                        >  1:265393/1‑148 (MQ=255)
                                                       aCCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcacc                                                                                                              <  1:743984/127‑1 (MQ=255)
                                                       aCCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAagcacc                                                                                                              >  2:743984/1‑127 (MQ=255)
                                                       aCCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGgcg                                                                                        >  1:177356/1‑149 (MQ=255)
                                                         cTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCACCGGCGCt                                                                                      >  2:1327216/1‑149 (MQ=255)
                                                           aaTGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTc                                                                                    >  2:1204567/1‑149 (MQ=255)
                                                           aaTGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTc                                                                                    >  2:1043983/1‑149 (MQ=255)
                                                             tGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCgg                                                                                  <  1:1511761/149‑1 (MQ=255)
                                                              gCCTGCATCAGCGCGCTGGATTTTTCCGCGAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGc                                                                                 <  1:223114/149‑1 (MQ=255)
                                                              gCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATAACCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGc                                                                                 <  1:105802/149‑1 (MQ=255)
                                                                  gCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCcaca                                                                             >  2:1527038/1‑149 (MQ=255)
                                                                     tCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATc                                                                          <  2:1222443/149‑1 (MQ=255)
                                                                       aGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATccc                                                                        >  2:204214/1‑149 (MQ=255)
                                                                       aGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATccc                                                                        <  1:355689/149‑1 (MQ=255)
                                                                            gcTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCg                                                                   >  2:531720/1‑149 (MQ=255)
                                                                             cTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGt                                                                  <  2:1207983/149‑1 (MQ=255)
                                                                              tGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATACCCCCTACCAGACCGTAGACGCCCACGGTCCCTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCACCGGCGCTTCGGCCACAATCCACcgcggg                                                                 >  2:37912/1‑147 (MQ=255)
                                                                                 aTTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATg                                                              >  1:476391/1‑149 (MQ=255)
                                                                                 aTTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATg                                                              >  1:746738/1‑149 (MQ=255)
                                                                                  tttttCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATgg                                                             >  1:377474/1‑149 (MQ=255)
                                                                                  tttttCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATgg                                                             <  1:784428/149‑1 (MQ=255)
                                                                                         gccagccaATTCCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATCCCTGAAAGCCCCAGCACCTGAGTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGGGACCAt                                                      >  1:329002/1‑149 (MQ=255)
                                                                                          ccagccaATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGAtt                                                     <  1:1546610/149‑1 (MQ=255)
                                                                                                aaTACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCa                                               <  1:531512/149‑1 (MQ=255)
                                                                                                 aTACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCATCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAg                                              <  2:505043/149‑1 (MQ=255)
                                                                                                     cccAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAACGCCCCAGCACCTGATTAAGCAACGGCGCTGCGGCCACAATCCCCAGCGTGCTGGCGCCGAGTTCCGCAGACaa                                          >  1:49534/1‑149 (MQ=255)
                                                                                                     cccAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACaa                                          >  2:1328867/1‑149 (MQ=255)
                                                                                                     cccAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACaa                                          <  1:894747/149‑1 (MQ=255)
                                                                                                             atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAAt                                  >  1:169197/1‑149 (MQ=255)
                                                                                                             atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAAt                                  >  1:744975/1‑149 (MQ=255)
                                                                                                             atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAAt                                  <  1:753830/149‑1 (MQ=255)
                                                                                                             atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTAAATACCCGCGCGATGACAGCAAGAACCAGAACCTGATTACGACACGGCGCTTCTGACACAATACACAGCGAGATGTCGACGATTTCCGAAGAAAAAATaaaaa                                  >  2:213522/1‑148 (MQ=255)
                                                                                                             atcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACCGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGGAGACAATATAAAAt                                  <  1:236327/149‑1 (MQ=255)
                                                                                                              tcaATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATc                                 <  1:435563/149‑1 (MQ=255)
                                                                                                               caATCTTAACGATAACCCCTACCAGACCGTAGACGCACACGGTCACTACCAGCGCGATGCCTGAAAGCACCCGCGCCAGATTAAGCAACGGCGCTTCGGCCACAATCCCCCGCCTGATGGCGACGATTTCCGCAGACAATATAAAAACg                                >  1:641359/1‑149 (MQ=255)
                                                                                                                 aTCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGt                              >  1:1092689/1‑149 (MQ=255)
                                                                                                                   cTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTAc                            <  1:375394/149‑1 (MQ=255)
                                                                                                                   cTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTAc                            <  1:1428913/149‑1 (MQ=255)
                                                                                                                       aCGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAAt                        >  1:691006/1‑149 (MQ=255)
                                                                                                                       aCGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAAt                        >  1:1243643/1‑149 (MQ=255)
                                                                                                                          aTAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGAGATGGCGACGATTTCCGCAGACAATATATAATCGGTACGAATCGc                     >  1:31547/1‑149 (MQ=255)
                                                                                                                               cccTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCtt                >  1:1507707/1‑149 (MQ=255)
                                                                                                                                 cTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCtttt              >  1:611371/1‑149 (MQ=255)
                                                                                                                                   tagtgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGTCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAt            <  2:244301/145‑1 (MQ=255)
                                                                                                                                   tagtgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAt            >  1:1247690/5‑149 (MQ=255)
                                                                                                                                    ccAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCATTTTAtt           >  1:691103/1‑149 (MQ=255)
                                                                                                                                      tgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAtttt         >  1:865497/2‑149 (MQ=255)
                                                                                                                                      tgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAtttt         >  2:654281/2‑149 (MQ=255)
                                                                                                                                      tgACCGTGGGCGTCTACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTAtttt         >  1:578528/2‑149 (MQ=255)
                                                                                                                                       gACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCTGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTg        >  2:675319/1‑149 (MQ=255)
                                                                                                                                       gACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTg        >  2:213960/1‑149 (MQ=255)
                                                                                                                                         ccGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTc      <  2:625662/149‑1 (MQ=255)
                                                                                                                                         ccGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTc      <  2:746738/149‑1 (MQ=255)
                                                                                                                                         ccGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTc      <  2:476391/149‑1 (MQ=255)
                                                                                                                                            tAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCtt   <  1:746772/149‑1 (MQ=255)
                                                                                                                                             aGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCttt  <  1:384842/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
TGCCGACAATCGATAACGCTTTCATCAGCCAGGGCGCGATAATCAATAACCCTTTACCTAATGCCTGCATCAGCGCGCTGGATTTTTCCGCCAGCCAATACCCCAGGTCATCAATCTTAACGATAACCCCTACCAGACCGTAGACGCCCACGGTCACTACCAGCGCGATGCCTGAAAGCACCAGCACCTGATTAAGCAACGGCGCTTCGGCCACAATCCCCAGCGTGATGGCGACGATTTCCGCAGACAATATAAAATCGGTACGAATCGCCCCTTTTATTTTGTCCTTT  >  NC_000913/2028658‑2028947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: