Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	314,595	G→C	7.5%	pseudogene (239/872 nt)	eaeH →	pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	314,595	0	G	C	7.5%	248.1 / 11.0	80	pseudogene (239/872 nt)	eaeH	pseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base G (27/47);  new base C (3/3);  total (30/50)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-01

GTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGAT  >  NC_000913/314447‑314737                                                                                                                                                     |                                                                                                                                               gTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACTGTAACTGCTGATAATAACGTGGAGAAAAATGACGCGTCGTTTGCCGCAAATGCCgg                                                                                                                                                >  2:978269/1‑149 (MQ=255)  ttCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATTACGTGGAGATAAATGTCGCGTCGTTTGCCGCTTATGCCggg                                                                                                                                               >  1:629261/1‑149 (MQ=255)  ttCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCggg                                                                                                                                               >  2:1586033/1‑149 (MQ=255)   tCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGa                                                                                                                                              <  2:1528702/149‑1 (MQ=255)   tCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGa                                                                                                                                              <  1:598328/149‑1 (MQ=255)    cTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACTTCGAGAAAAATGTCGCGTCGTATGCCGCAAATGCCGGGAc                                                                                                                                             >  2:248610/1‑149 (MQ=255)       tttCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTGCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTGTGCCGCAAATGCCGGGACAtt                                                                                                                                          <  2:641643/149‑1 (MQ=255)        ttCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACAttt                                                                                                                                         <  2:598084/149‑1 (MQ=255)          ccACTCGCTGTCCCCTTTACCCCAGTAATGGCGGCACTTGCGCAGCATGCGGGTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAttttt                                                                                                                                       <  2:1149062/149‑1 (MQ=37)          ccACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAttttt                                                                                                                                       <  2:1494512/149‑1 (MQ=38)           cACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCCCGGCATTTGCGGCAAACGACGCGACAtttttc                                                                                                                                      <  2:527536/149‑2 (MQ=38)            aCTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTaa                                                                                                                                     <  2:1444087/149‑1 (MQ=255)             cTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                                    <  2:1535897/149‑1 (MQ=255)             cTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                                    >  2:1227641/1‑149 (MQ=255)             cTCGCTGGCACCTTTACCCCAGAAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAg                                                                                                                                    <  1:365269/149‑1 (MQ=255)               cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCa                                                                                                                                  <  1:670290/149‑1 (MQ=255)               cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCa                                                                                                                                  <  1:1517673/149‑1 (MQ=255)               cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCa                                                                                                                                  <  2:653514/149‑1 (MQ=255)                      aCCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGcc                                                                                                                           >  2:1282721/1‑149 (MQ=255)                      aCCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGcc                                                                                                                           <  2:681544/149‑1 (MQ=255)                       ccTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGCGCAGCCACGGGTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCa                                                                                                                          <  1:930139/149‑1 (MQ=255)                           tACCCCAGTAATGGCGGCACGGGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATa                                                                                                                      <  2:169092/149‑1 (MQ=255)                              cccAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCg                                                                                                                   >  1:1463218/1‑149 (MQ=255)                              cccAGTAATGGCGGCACGTGCGCAGCATGCGGTTAAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCg                                                                                                                   >  2:958529/1‑149 (MQ=255)                                cAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                                 <  1:1224959/149‑1 (MQ=255)                                cAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                                 >  1:1472424/1‑149 (MQ=255)                                cAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGAt                                                                                                                 <  1:667921/149‑1 (MQ=255)                                 aGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATg                                                                                                                <  1:972749/149‑1 (MQ=255)                                  gTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGc                                                                                                               <  1:1257966/149‑1 (MQ=255)                                    aaTGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGa                                                                                                             <  2:665147/149‑1 (MQ=255)                                           gcACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCCTGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTaa                                                                                                      <  1:1267052/149‑1 (MQ=255)                                              agTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGTGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACtt                                                                                                   <  2:868142/148‑1 (MQ=255)                                                tGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATTACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGGCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                                 <  2:229981/149‑1 (MQ=255)                                                tGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTACTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                                 >  1:1273781/1‑149 (MQ=255)                                                     agcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTAcc                                                                                            >  2:815561/1‑149 (MQ=255)                                                     agcaTGCGGCTCAGCCACGGTGGAGCATGGGAAATACTACTGTAACTGCTGATAATAACGTGGAGAAATCTGTCGCGTCGTTTGCCGCAAATGCCGGGACATGTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTAcc                                                                                            <  1:982015/149‑1 (MQ=255)                                                      gcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCg                                                                                           >  2:106277/1‑149 (MQ=255)                                                      gcaTGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCg                                                                                           <  1:1297517/149‑1 (MQ=255)                                                            ggTGCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATgg                                                                                     <  2:569508/149‑1 (MQ=255)                                                             gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGc                                                                                    >  2:400137/1‑149 (MQ=255)                                                             gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGc                                                                                    >  1:1230415/1‑149 (MQ=255)                                                             gTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGc                                                                                    >  2:795635/1‑149 (MQ=255)                                                               tctgCCACGGTTGAGCATTGGAAATAATACTGTAAATGCTTATTATAACGTGGAGAAAAATGTAGAGTCGGTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCca                                                                                  <  2:1430818/146‑1 (MQ=255)                                                                        gTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACagctaaag                                                                         <  2:187128/149‑1 (MQ=255)                                                                             gCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAAc                                                                    <  2:1382062/149‑1 (MQ=255)                                                                              cATGGGAATTACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCAACAGCTAAAGCTAAcc                                                                   >  2:1451210/1‑149 (MQ=255)                                                                               aTGGGAAATACTACGGTAACTGCTGATAATTACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTTCCGGAATGGCCACAGCTAAAGCTAACCa                                                                  <  2:1570621/149‑1 (MQ=255)                                                                               aTGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCa                                                                  <  2:923534/149‑1 (MQ=255)                                                                                tGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAg                                                                 <  1:1576907/149‑1 (MQ=255)                                                                                    aaaTACTACGGTAACTGATGATAATAACGTCGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACATCTAAAGCTAACCAGGaaa                                                             <  1:1517373/149‑1 (MQ=255)                                                                                       tactacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATAc                                                          >  1:1496565/1‑149 (MQ=255)                                                                                        actacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGGCCTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACa                                                         <  2:322192/149‑1 (MQ=255)                                                                                         ctacTGTAACTGCTGATAATAAAGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAg                                                        <  2:491561/149‑1 (MQ=255)                                                                                          tacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                                 <  1:1052437/107‑1 (MQ=255)                                                                                          tacGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTtta                                                                                                 >  2:1052437/1‑107 (MQ=255)                                                                                           acGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGa                                                      <  1:653597/149‑1 (MQ=255)                                                                                              gTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTg                                                   >  2:1343454/1‑149 (MQ=255)                                                                                                aaCTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGAGATGAGACACGTAACTGTATTACCGGAATGGCCACAGCTAAAGCTATCGAGGAATTACAGGAGTgga                                                 >  2:207436/1‑148 (MQ=255)                                                                                                  ctgctgATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTc                                               <  2:1545100/149‑1 (MQ=255)                                                                                                   tgctgATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCg                                              >  1:605513/1‑149 (MQ=255)                                                                                                        ataataACGTGGAGAAAAATGTCGCGTCGTTTGACGCAAATGACGGGACATTTTTAAGAAGACAGCCAGATAGCGATGCGACTCGTAACTTTATTACCTGAATGTAAACAGCTAAATCTAAACAGTAAATACATTAGTGGATCGGTaaa                                         >  1:988973/1‑149 (MQ=255)                                                                                                          aataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGAGATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTAATTACCGGAATGGCCACAGCTAAAGCTAACAAGGAAATACAGGAGAGGCTCGGGAAata                                       >  2:609302/1‑149 (MQ=255)                                                                                                          aataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAata                                       <  1:178404/149‑1 (MQ=255)                                                                                                           ataaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAatat                                      >  1:1051050/1‑149 (MQ=255)                                                                                                            taaCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATg                                     <  1:10704/149‑1 (MQ=255)                                                                                                               cGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTTGGGAAATATGGTa                                  <  1:106277/149‑1 (MQ=255)                                                                                                               cGTGGAGAAAAATGTCGCGGCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTa                                  <  2:1155907/149‑1 (MQ=255)                                                                                                                gTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTAc                                 >  2:609088/1‑149 (MQ=255)                                                                                                                 tgcttaaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACt                                >  1:19371/6‑149 (MQ=37)                                                                                                                    ttaaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcg                             >  2:484492/3‑149 (MQ=38)                                                                                                                      aaaaaTGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAAGTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCACGGGAAATATGGTACTgcgcg                           >  2:875986/1‑149 (MQ=255)                                                                                                                      aaaaaTGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcgcg                           >  1:553622/1‑149 (MQ=255)                                                                                                                      aaaaaTGTCCCGGCATTTGCGGCAAACGACGCGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTgcgcg                           >  2:721573/1‑149 (MQ=38)                                                                                                                            gTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCaa                     >  2:1376189/1‑149 (MQ=255)                                                                                                                             tCGCGTCGTTTGCCGCAAATGCCGGTACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCaaa                    <  1:978269/149‑1 (MQ=255)                                                                                                                             tCGCGTCGTTTGCAGCAAATTCCGGGACATTATTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCaaa                    <  2:817048/149‑1 (MQ=255)                                                                                                                               gcgTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACt                  >  1:594956/1‑149 (MQ=255)                                                                                                                                       tGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCg          <  2:1472424/149‑1 (MQ=255)                                                                                                                                        gCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTCCCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGa         <  1:1282721/149‑1 (MQ=255)                                                                                                                                            cAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATaaa     <  2:454130/149‑1 (MQ=255)                                                                                                                                            cAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATaaa     <  1:1545512/149‑1 (MQ=255)                                                                                                                                             aaaTGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGAGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATAAGGTACTCCGCGCGTCAAACTGAATGTCGATAAAg    >  1:324270/1‑149 (MQ=255)                                                                                                                                               aTGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGAt  <  1:1376189/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               GTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGAT  >  NC_000913/314447‑314737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑