Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,259,550 A→T 6.2% intergenic (‑254/+169) psuK ← / ← fruA pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,259,5500AT 6.2% 480.7 / 14.5 155intergenic (‑254/+169)psuK/fruApseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base A (71/74);  new base T (4/6);  total (75/80)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 171 total aligned reads displayed.

TGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCT  >  NC_000913/2259403‑2259698
                                                                                                                                                   |                                                                                                                                                    
tGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGaaaa                                                                                                                                                     >  2:108890/1‑149 (MQ=255)
 gCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAaat                                                                                                                                                    >  2:1485147/1‑149 (MQ=255)
 gCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAaat                                                                                                                                                    >  1:661142/1‑149 (MQ=255)
 gCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAaat                                                                                                                                                    >  1:1499594/1‑149 (MQ=255)
 gCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATAGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAAGGCAGAAaat                                                                                                                                                    >  2:1552269/1‑149 (MQ=255)
   aTGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAaataa                                                                                                                                                  <  2:277812/149‑1 (MQ=255)
      ttttACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCTGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCAAAGAACAGAAAAATAAAAAAGGGCAGAAAATAATcg                                                                                                                                               >  1:258126/1‑148 (MQ=255)
       tttACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAATGGCAGAAAATAATCTg                                                                                                                                              >  2:376020/1‑149 (MQ=255)
          atgtaaagttaaTTACAGCCAGATAGTCAAAATGGGCAGATTTGAGAAGGCTCACAATATGCGTTTATATGCCTGAATTACAAAAGCGAGGAATTTGTGTCGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTGccc                                                                                                                                           <  2:1277989/147‑1 (MQ=255)
          aCGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTGccc                                                                                                                                           <  2:1512862/149‑1 (MQ=255)
              aaagtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAACGGGCAGATTATTTTCTGCCCtttt                                                                                                                                       <  2:879218/149‑3 (MQ=255)
               aagtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAg                                                                                                                                      <  2:406275/149‑1 (MQ=255)
                agtaaaTTACCGCCAGAGAGGCAAATTGGGCAGATTTGAACAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAgg                                                                                                                                     >  1:1562771/1‑149 (MQ=255)
                  taaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGaa                                                                                                                                   >  2:1342798/1‑149 (MQ=255)
                    aaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAAt                                                                                                                                 <  1:48335/149‑1 (MQ=255)
                      ttACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATaa                                                                                                                               >  2:737921/1‑149 (MQ=255)
                           gCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGa                                                                                                                          <  1:739264/149‑1 (MQ=255)
                              agagagGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAgtg                                                                                                                       >  2:851510/1‑149 (MQ=255)
                                   ggCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtga                                                                                                                  <  1:84170/149‑1 (MQ=255)
                                    gCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCAAAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGTAAATAACCTGAGTGTTtgat                                                                                                                 >  2:143064/1‑149 (MQ=255)
                                            ggCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGttt                                                                                                         <  2:625107/149‑1 (MQ=255)
                                              cAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGa                                                                                                       >  2:1174164/1‑149 (MQ=255)
                                               agttttGAGCATGCTCACAAAATGCGATTTAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGaa                                                                                                      <  2:229408/146‑1 (MQ=255)
                                               aGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGaa                                                                                                      <  2:423869/149‑1 (MQ=255)
                                               aGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGaa                                                                                                      <  2:683052/149‑1 (MQ=255)
                                                gATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAGAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAg                                                                                                     <  1:38254/149‑1 (MQ=255)
                                                gATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATTATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAg                                                                                                     <  1:349875/149‑1 (MQ=255)
                                                 ttttGATCAGGCTCACAAAATGCGATTAAATGCCTTAATTAAAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGt                                                                                                    <  1:790730/148‑1 (MQ=255)
                                                 aTTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGCACAGAACAATAAAAAAGGGCAGCAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTATGAAGt                                                                                                    >  1:250821/1‑149 (MQ=255)
                                                   ttGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGttt                                                                                                  <  1:155434/149‑1 (MQ=255)
                                                     gAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCt                                                                                                >  2:30059/1‑149 (MQ=255)
                                                      aGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTa                                                                                               <  2:649689/149‑1 (MQ=255)
                                                        cAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAACGGGCAGAAAATAATATGCCCTTGAGGAAATAACCTGATTGTTTGATGAACGTTTGAAGTTTCTaat                                                                                             >  2:112482/1‑147 (MQ=255)
                                                           gCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGaa                                                                                          >  2:1017325/1‑149 (MQ=255)
                                                           gCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGaa                                                                                          >  1:970936/1‑149 (MQ=255)
                                                            cTCACAAAATGCGATTAAATGCCTTAATTACAATAGCGAGGAATTTGTGTCGGGATGCACAGAAAAGAACAATAATAAAGGGGAGAAAATAATCTTCCCTTGAGGAAATATCCTGAGTGTGTGATGAACGTTTGAAGTTTCTACTGaaa                                                                                         <  1:207066/149‑1 (MQ=255)
                                                               tctaaaTGCGATTAAATGCCTGATTTACAAAAGCGAGGAATTTGTGACGGGTTGCACAGCACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCGTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGAt                                                                                      >  2:1233212/4‑149 (MQ=255)
                                                               acaAAATGCGATTAAATGCCTGAATAACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGAt                                                                                      <  1:1090843/149‑1 (MQ=255)
                                                                  aaaTGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATaaa                                                                                   >  2:1596660/1‑149 (MQ=255)
                                                                    aTGCGATTAAATGCCTGAATTACTAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATATAAAACGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGTAAGATTAAAc                                                                                 <  2:641499/149‑1 (MQ=255)
                                                                    aTGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAAc                                                                                 <  1:1290308/149‑1 (MQ=255)
                                                                     tGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAAcc                                                                                <  2:152426/149‑1 (MQ=255)
                                                                       cGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCAt                                                                              >  2:139036/1‑149 (MQ=255)
                                                                         aTTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCAttt                                                                            <  2:952760/149‑1 (MQ=255)
                                                                                 ccTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCAt                                                                    >  2:851972/1‑149 (MQ=255)
                                                                                  cTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATa                                                                   >  2:1465829/1‑149 (MQ=255)
                                                                                  cTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATa                                                                   >  1:1113585/1‑149 (MQ=255)
                                                                                   tGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGAAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATaa                                                                  <  1:1441613/149‑1 (MQ=255)
                                                                                     aaTTACAAAAGCGAGTAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATaaaa                                                                <  1:1164706/149‑1 (MQ=255)
                                                                                     aaTTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATaaaa                                                                <  1:737921/149‑1 (MQ=255)
                                                                                     aaTTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATaaaa                                                                >  2:687346/1‑149 (MQ=255)
                                                                                     aaTTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATaaaa                                                                >  1:170709/1‑149 (MQ=255)
                                                                                       ttACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAAtt                                                              >  1:1358838/1‑149 (MQ=255)
                                                                                         aCAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTAc                                                            <  1:851510/149‑1 (MQ=255)
                                                                                         aCAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTAc                                                            >  1:1531856/1‑149 (MQ=255)
                                                                                          cAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACa                                                           <  1:952530/149‑1 (MQ=255)
                                                                                          cAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACa                                                           <  2:1292827/149‑1 (MQ=255)
                                                                                           aaaaGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACAt                                                          <  1:1247512/149‑1 (MQ=255)
                                                                                             aaGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAATGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTc                                                        <  1:376020/149‑1 (MQ=255)
                                                                                              aGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGTCAGAAAATAATCTGCACTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTca                                                       <  1:198/149‑1 (MQ=255)
                                                                                              aGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTca                                                       >  1:940876/1‑149 (MQ=255)
                                                                                                ctaGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTcaca                                                     >  2:404936/3‑149 (MQ=255)
                                                                                                   ggAATTTGTGACGGGATTCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTCGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAc                                                  <  2:816362/149‑1 (MQ=255)
                                                                                                   ggAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCCGAAAATAATCTGCACTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAc                                                  <  2:250821/149‑1 (MQ=255)
                                                                                                   ggAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAc                                                  <  1:447182/149‑1 (MQ=255)
                                                                                                          gtgACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCAt                                           >  1:488825/1‑149 (MQ=255)
                                                                                                           tgACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCAtt                                          <  1:858469/149‑1 (MQ=255)
                                                                                                            gACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACaaa                                                   <  2:965225/139‑1 (MQ=255)
                                                                                                             aCGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATtct                                        <  2:383656/149‑1 (MQ=255)
                                                                                                             aCGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAATTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATtct                                        >  2:442522/1‑149 (MQ=255)
                                                                                                              cGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATtctc                                       >  1:691366/1‑149 (MQ=255)
                                                                                                                  aTGCACAGAACAGAACAATAAAAAAGGGCAGAATATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTg                                   <  1:681768/149‑1 (MQ=255)
                                                                                                                    gCAGAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTgcg                                 <  1:923501/149‑1 (MQ=255)
                                                                                                                      acagaacagaACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCt                               >  1:1439779/1‑149 (MQ=255)
                                                                                                                       cagaacagaaCAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACGAACCGGTCATTCTCTTTGCGCtc                              >  2:121092/1‑149 (MQ=255)
                                                                                                                        agaacagaacAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCtca                             <  1:933535/149‑1 (MQ=255)
                                                                                                                          aacagaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCtcatc                           >  2:1083044/1‑149 (MQ=255)
                                                                                                                             agaacaATAAAAAAGGGCAGAAAATAATCTGCACTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCaaa                        <  1:159563/149‑1 (MQ=255)
                                                                                                                              taacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAAt                       <  1:569378/148‑1 (MQ=255)
                                                                                                                               aacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATg                      >  2:1550759/1‑149 (MQ=255)
                                                                                                                                 caATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGtt                    >  1:512540/1‑149 (MQ=255)
                                                                                                                                  aaTAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTa                   >  2:979679/1‑149 (MQ=255)
                                                                                                                                  aaTAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTa                   >  1:1244602/1‑149 (MQ=255)
                                                                                                                                  aaTAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTa                   <  2:1084397/149‑1 (MQ=255)
                                                                                                                                   aTAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTac                  >  1:1208881/1‑149 (MQ=255)
                                                                                                                                       tcaaGGGCAGATTATTTTCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacagga              >  1:180041/3‑149 (MQ=255)
                                                                                                                                       aaaaGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacagga              >  2:404615/1‑149 (MQ=255)
                                                                                                                                         aaGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCa                                            >  2:550864/1‑117 (MQ=255)
                                                                                                                                         aaGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacaggaca            <  2:1562771/149‑1 (MQ=255)
                                                                                                                                          aGGGCAGATTATTTTCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacaggacag           >  1:980325/1‑149 (MQ=255)
                                                                                                                                           gggCAGATTATTTTCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacaggacagg          >  1:1357937/1‑149 (MQ=255)
                                                                                                                                            ggCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacagtcgggtt         >  1:1430074/1‑142 (MQ=255)
                                                                                                                                            ggCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTAcaggacagga         <  1:1017325/149‑1 (MQ=255)
                                                                                                                                             gCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGaa        >  2:1314211/1‑149 (MQ=255)
                                                                                                                                               aGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGGATTAAGTTTCTACTTAAAGATAAAACCATAAAAATTCATAAAATTACATACACAAACCGGTCATTCTCTTTGCCGTCAACAAATCTTACAGGAAAGGACAt      >  2:938619/1‑149 (MQ=255)
                                                                                                                                                 aaaaTAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacag                >  2:185670/1‑137 (MQ=255)
                                                                                                                                                 aaaaTAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAAttt    <  1:1083840/149‑1 (MQ=255)
                                                                                                                                                  aaaTAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTc   <  2:1531856/149‑1 (MQ=255)
                                                                                                                                                   aataatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCt  >  1:1324712/1‑149 (MQ=255)
                                                                                                                                                   aataatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCt  <  2:609773/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCT  >  NC_000913/2259403‑2259698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: