Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,392,498 C→G 5.0% G182R (GGC→CGC)  nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,392,4980CG 5.0% 384.4 / 9.8 119G182R (GGC→CGC) nuoMNADH:ubiquinone oxidoreductase, membrane subunit M
Reads supporting (aligned to +/- strand):  ref base C (60/52);  new base G (2/4);  total (63/56)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

Only 100 of 120 total aligned reads displayed.

AGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCA  >  NC_000913/2392351‑2392646
                                                                                                                                                   |                                                                                                                                                    
aGGCGATGAAGAAGCCCAGCATTAACAGGTATTACACACAACTGGACATTGGCGGATTCAGCAGCTCTTCATAGTTGAACGTCCAGACGCCGGTCGCATAGTAGTGAACCAAAACCACCCCCAGGAACGCAAACAACAACACCAGGGCa                                                                                                                                                     >  2:158356/1‑149 (MQ=255)
    gATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCg                                                                                                                                                 >  1:1348526/1‑149 (MQ=255)
    gATGAAGAAGCCCACCATTAACAGGTATACCACACCACTGGCCATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTACTGAACAAAAACCAGCGCCAGGATGGCAATCAAAATCACCAGCCCACTcc                                                                                                                                                 >  1:1312046/1‑148 (MQ=255)
     aTGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGc                                                                                                                                                <  2:1352333/149‑1 (MQ=255)
     aTGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCATATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGc                                                                                                                                                >  2:346199/1‑149 (MQ=255)
        aagaagCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCGAGTGGCCTg                                                                                                                                             <  2:743905/149‑1 (MQ=255)
         agaagCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTgg                                                                                                                                            >  2:920531/1‑149 (MQ=255)
         agaagCCCAGCATTAACAGGTATTCCAAACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTgg                                                                                                                                            <  1:845833/149‑1 (MQ=255)
          gaagCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTggg                                                                                                                                           >  1:1565534/1‑149 (MQ=255)
             gCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGgtgt                                                                                                                                        >  2:1206500/1‑149 (MQ=255)
              cccAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCGCTTCATAGTTGACGGTCCAGACGCCGGGCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCCGGCGAGTGGCCTggtgatg                                                                                                                                       <  1:17164/149‑6 (MQ=255)
               ccAGCTTTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGAGGCCGGGCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCGAGTGGCCTggtgatgt                                                                                                                                      <  1:86912/149‑7 (MQ=255)
               ccAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTaa                                                                                                                                      >  1:546077/1‑149 (MQ=255)
                cAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTaaa                                                                                                                                     >  2:19876/1‑149 (MQ=255)
                cAGCATTAACAGGTATTCCACACCACTCGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTaaa                                                                                                                                     <  1:1288526/149‑1 (MQ=255)
                 aGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTTAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACGCGCCTGGGTGTAAAt                                                                                                                                    >  1:973042/1‑149 (MQ=255)
                        aCAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaagaaa                                                                                                                             >  2:686501/1‑148 (MQ=255)
                            gTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTgg                                                                                                                         >  2:727387/1‑149 (MQ=255)
                              aTTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGtt                                                                                                                       <  1:156183/149‑1 (MQ=255)
                                     accacTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGccgccg                                                                                                                >  1:523361/1‑149 (MQ=255)
                                       cacTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTg                                                                                                              >  1:575891/1‑149 (MQ=255)
                                          tGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATa                                                                                                           <  1:1053770/149‑1 (MQ=255)
                                           ggACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAc                                                                                                          >  1:494230/1‑149 (MQ=255)
                                           ggACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAc                                                                                                          >  2:202764/1‑149 (MQ=255)
                                               aTTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt                                                                                                      <  2:1596783/149‑1 (MQ=255)
                                               aTTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt                                                                                                      >  1:1189099/1‑149 (MQ=255)
                                                ttGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAATACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGtt                                                                                                     <  1:1189327/149‑1 (MQ=255)
                                                 tGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGttt                                                                                                    <  2:531136/149‑1 (MQ=255)
                                                   gCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTa                                                                                                  >  1:725597/1‑149 (MQ=255)
                                                     ttATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc                                                                                                <  1:346199/148‑1 (MQ=255)
                                                        ttCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTc                                                                                             >  1:113797/1‑149 (MQ=255)
                                                         tCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCa                                                                                            <  1:202764/149‑1 (MQ=255)
                                                           agcagcTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCaga                                                                                          >  2:1541350/1‑149 (MQ=255)
                                                           agcagcTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACAAGCGCCAGGATGGCAATCACCATCACCAGGCCACTCGCCTGGGTCTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagc                                                                                          >  1:1216910/1‑148 (MQ=255)
                                                           agcagcTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGGGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTGTTACCGTCagc                                                                                          >  1:1048399/1‑148 (MQ=255)
                                                             cagcTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGCTACGCGTTTTACCGTCAGAgg                                                                                        >  2:6403/1‑149 (MQ=255)
                                                                ctctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt                                                                                     <  1:1372007/149‑1 (MQ=255)
                                                                 tctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCttt                                                                                    >  2:278923/1‑149 (MQ=255)
                                                                  ctTCATCGTGGCAGTGCCAGACGCCGGTCGCATTGTTTGGAACACAAACCAGCGCCAGGATGGCAATCAACCTCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGGCAGAGGCTTTa                                                                                   <  2:811356/149‑1 (MQ=255)
                                                                   ttCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATTAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt                                                                                  >  2:840578/1‑149 (MQ=255)
                                                                   ttCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt                                                                                  <  1:1336856/149‑1 (MQ=255)
                                                                   ttCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt                                                                                  >  1:794005/1‑149 (MQ=255)
                                                                    tCATAGTTGAAGGTCCTGACGCCGGTCGCATTGTAGTGAACAAAATCAAGCGCCAGGATGGCAATGAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGGTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATg                                                                                 <  1:1525603/149‑1 (MQ=255)
                                                                    tCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATg                                                                                 >  1:1368973/1‑149 (MQ=255)
                                                                      aTAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATTcc                                                                               >  1:1501969/1‑149 (MQ=255)
                                                                          tttaaGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCTATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCca                                                                           <  1:581871/146‑1 (MQ=255)
                                                                             aaGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAg                                                                        >  1:971677/1‑149 (MQ=255)
                                                                                gTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGc                                                                     <  1:1206500/149‑1 (MQ=255)
                                                                                gTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGc                                                                     >  1:972324/1‑149 (MQ=255)
                                                                                 tCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCg                                                                    >  1:375714/1‑149 (MQ=255)
                                                                                   cAGACGCCGGTCGCATTGTAGTGAACAAAAACCCGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGAt                                                                  >  2:1067102/1‑149 (MQ=255)
                                                                                   aagtcGCCGGTCGCATTGTAGTGTAAATAATCCAGCGCCTTGATGGCAATCAACATCACCAGGCCACTCGCCTGGTTGTAAATGAAGAACTTTGTTGCCGCCTTGATACGCGTTTTACCGTCAGATGCTTTATGCCCCCACAGTGCGAt                                                                  <  2:739299/145‑1 (MQ=255)
                                                                                     gACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTTATTCGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCa                                                                <  1:1276351/149‑1 (MQ=255)
                                                                                     gACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCa                                                                >  2:947374/1‑149 (MQ=255)
                                                                                      actccGGTATCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCTCTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGACGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCTATCAg                                                               <  2:726945/146‑1 (MQ=255)
                                                                                       cGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAgg                                                              <  1:920531/149‑1 (MQ=255)
                                                                                       cGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAgg                                                              <  2:1348526/149‑1 (MQ=255)
                                                                                        gCCGGTCGGTTTTTAGTGAACAAAAACCTGCGCCAGGATGGCAATCATCATCACCAGGCCACTCGCGTGGGTGTATATGAAGAACTTGGTTGCCGCCGTGATTCGCGTTTTAGCGTCAGAGGCTTTTTGCCCCCTCAGTGCGATCAGGa                                                             <  2:1312046/149‑1 (MQ=255)
                                                                                        gCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGCACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa                                                             >  1:928915/1‑149 (MQ=255)
                                                                                         ccGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGaa                                                            <  1:33260/149‑1 (MQ=255)
                                                                                          cGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAg                                                           <  2:178215/149‑1 (MQ=255)
                                                                                            gtggCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGAGTAAATGAAGAACTTGGTTGCCGCCGTGAGACGCGTTTTACCGTCAGCGGCTTTATGCCCCCACAGTGCGATCAGGAAGTa                                                         >  1:234213/4‑149 (MQ=255)
                                                                                               gCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACAt                                                      <  2:761486/149‑1 (MQ=255)
                                                                                               gCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACAt                                                      <  2:766875/149‑1 (MQ=255)
                                                                                               gCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAAACAACATCACCAGGACACTCGCCTGGGTGTAAATGAAGACCTTCGTTGCCGCCGTGAAACGCGTTTTCCCCTCAGAGGCTTTATTCCCCCACAGTGCGATCAGGAAGTActt                                                      >  1:267360/1‑147 (MQ=255)
                                                                                                 aTTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCg                                                    >  1:694480/1‑149 (MQ=255)
                                                                                                   tGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGc                                                  <  2:971677/149‑1 (MQ=255)
                                                                                                     tAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCAc                                                <  2:1208632/149‑1 (MQ=255)
                                                                                                       gTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACCTCGGCACCa                                              >  2:1475329/1‑149 (MQ=255)
                                                                                                       gTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCa                                              >  2:1481243/1‑149 (MQ=255)
                                                                                                       gTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCa                                              >  2:518783/1‑149 (MQ=255)
                                                                                                       gTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTGTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCa                                              >  1:801695/1‑149 (MQ=255)
                                                                                                       gTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACAAGGCAACTCGCCTGGGTGTAAATGAAGAACTTGGATGCAGCCTTGATACGCGTTTTACCGTCAGAGGATTTTAGCCCACAACGTGCGATCAGGAAGTACATCGGCACCa                                              >  1:421782/1‑149 (MQ=255)
                                                                                                        tGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAg                                             >  1:400549/1‑149 (MQ=255)
                                                                                                             aaaaaCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCTCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCCCCAGcttca                                        >  1:1000144/1‑149 (MQ=255)
                                                                                                             aaaaaCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGcatca                                        >  2:1333914/1‑149 (MQ=255)
                                                                                                                  ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTcc                                   <  2:1498122/149‑1 (MQ=255)
                                                                                                                  ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTcc                                   >  1:510007/1‑149 (MQ=255)
                                                                                                                  ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTcc                                   <  2:1331598/149‑1 (MQ=255)
                                                                                                                   cAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTccc                                  <  2:383732/149‑1 (MQ=255)
                                                                                                                    aGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTATATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCa                                 <  1:1293703/149‑1 (MQ=255)
                                                                                                                          aGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaa                           <  2:1368973/149‑1 (MQ=255)
                                                                                                                           ggATGGCAATCAAAATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGATGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATTCCCCCACAGTGCGATCAGGAAGTACATCGGCACAAGCATCATTTCCCagaagaag                          <  2:974448/149‑1 (MQ=255)
                                                                                                                            gagggCAATCAACATCACCATGCCACTCGCCTGTGTGTAAATGACGCACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaaga                         <  2:1048399/146‑1 (MQ=255)
                                                                                                                                 cAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAAc                    <  1:1581661/149‑1 (MQ=255)
                                                                                                                                    tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAgg                 <  1:959961/149‑1 (MQ=255)
                                                                                                                                    tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAgg                 >  1:1484912/1‑149 (MQ=255)
                                                                                                                                      aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGaa               <  2:113797/149‑1 (MQ=255)
                                                                                                                                          tCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACa                   <  1:53723/141‑1 (MQ=255)
                                                                                                                                          tCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACa                   >  2:53723/1‑141 (MQ=255)
                                                                                                                                           cACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGt          <  2:546077/149‑1 (MQ=255)
                                                                                                                                             ccAGGCGAGTGGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCg        >  1:1102572/1‑149 (MQ=255)
                                                                                                                                              cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGa       <  1:683791/149‑1 (MQ=255)
                                                                                                                                              cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGa       <  2:598614/149‑1 (MQ=255)
                                                                                                                                              cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGAGATACGCGTTTTACCTTCAGAGGCTTTATGCCCCCACAGTGCTATCAGGAAGTACATCGGCACCAGCATCATATCCCAGAAGAAGAAGAACAGGAACATgacca       >  2:309987/1‑145 (MQ=255)
                                                                                                                                                ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTAACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATg     >  1:1108075/1‑149 (MQ=255)
                                                                                                                                                 gCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTTATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCCCCAGCATCATTTCCCAGCAGAAGAAGAACAGGAACATGTCGATgg    >  2:277703/1‑149 (MQ=255)
                                                                                                                                                 gCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATgg    <  2:725597/149‑1 (MQ=255)
                                                                                                                                                 gCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATgg    >  1:1258417/1‑149 (MQ=255)
                                                                                                                                                   cACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCa  >  1:1557146/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCA  >  NC_000913/2392351‑2392646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: