Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,493,562 T→A 5.6% intergenic (‑308/+205) frc ← / ← yfdX formyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,493,5620TA 5.6% 518.9 / 14.0 162intergenic (‑308/+205)frc/yfdXformyl‑CoA transferase, NAD(P)‑binding/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (62/91);  new base A (3/6);  total (65/97)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

Only 100 of 170 total aligned reads displayed.

TTTCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATA  >  NC_000913/2493415‑2493709
                                                                                                                                                   |                                                                                                                                                   
tttCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGCAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCttttt                                                                                                                                                    >  1:837759/1‑149 (MQ=255)
tttCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACTCttttt                                                                                                                                                    >  1:52982/1‑149 (MQ=255)
tttCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCttttt                                                                                                                                                    >  1:1312847/1‑149 (MQ=255)
tttATGCATGTTAAGTAGTTAGGACGAAAAGAAGAATATGATAAATATTGATTAGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCttttt                                                                                                                                                    <  2:242538/149‑1 (MQ=255)
   ttGCATGGTAAGTATTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                                 <  1:1474409/148‑1 (MQ=255)
   cTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTaaa                                                                                                                                                 <  1:511244/149‑1 (MQ=255)
    tGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAg                                                                                                                                                <  2:1379835/149‑1 (MQ=255)
     gCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGc                                                                                                                                               >  1:1105440/1‑149 (MQ=255)
      cATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCg                                                                                                                                              >  1:174377/1‑149 (MQ=255)
        tGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTa                                                                                                                                            <  2:1299071/149‑1 (MQ=255)
        tGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTa                                                                                                                                            >  1:359673/1‑149 (MQ=255)
        tGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTa                                                                                                                                            <  1:452558/149‑1 (MQ=255)
         ggTAATTAGTGATGACGAAAATAAGAATATTATAAATATTGATTCGTGGGTATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCTCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaa                                                                                                                                           <  1:800901/149‑2 (MQ=255)
         ggTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaa                                                                                                                                           <  1:736499/149‑2 (MQ=255)
          gTAAGTAGTGAGGACGAAAAGATGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTAAAAAGCGTaat                                                                                                                                          <  2:1128577/149‑3 (MQ=255)
          gTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTAcc                                                                                                                                          <  2:321882/149‑1 (MQ=255)
            aaGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCAt                                                                                                                                        >  1:323177/1‑149 (MQ=255)
              gtagtGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATaa                                                                                                                                      <  2:1403158/149‑1 (MQ=255)
                agtGAGGACGAAAAGAATAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATTATTACGCTTTTTAAAGCGTACCATaaaa                                                                                                                                    <  1:1109425/149‑1 (MQ=255)
                    aGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATTATTACGCTTTTTAAAGCGTACCATAAaacata                                                                                                                                >  1:1338943/1‑149 (MQ=255)
                     tgACGAAAATAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGATTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataa                                                                                                                               <  1:950849/148‑1 (MQ=255)
                     ggACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAaacataa                                                                                                                               <  1:743539/149‑1 (MQ=255)
                         gAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAATAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAacataacata                                                                                                                           >  2:841868/1‑149 (MQ=255)
                         gAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTTAAGCGTACCATAAAacataacata                                                                                                                           >  2:903426/1‑149 (MQ=255)
                         gAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAacataacata                                                                                                                           >  2:876218/1‑149 (MQ=255)
                          aaaaGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAAcataacataa                                                                                                                          <  1:1581562/149‑1 (MQ=255)
                            aagaagAATATGATAAATATTGATTAGTGGTAATCAGAGAGAGTCGCTACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACataata                                                                                                                        <  2:805393/149‑1 (MQ=255)
                                  aaTATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGAt                                                                                                                  >  2:653303/1‑149 (MQ=255)
                                    tatGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGAttt                                                                                                                >  1:514574/1‑149 (MQ=255)
                                    tatGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGAttt                                                                                                                <  2:63713/149‑1 (MQ=255)
                                     atGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtg                                                                                                               >  1:1377035/1‑149 (MQ=255)
                                      tGATAAATATTGATTCTTTGAAATCTGAGAGATTCGAGAAATTTGTGATGTTATTATTACAACTTTAATAAGCGCCGGTGATTTACAAGCAATGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                              <  1:96927/149‑1 (MQ=255)
                                      tGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgt                                                                                                              <  2:31576/149‑1 (MQ=255)
                                       taTAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgtg                                                                                                             <  1:1242467/148‑1 (MQ=255)
                                       gATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgtg                                                                                                             <  2:920875/149‑1 (MQ=255)
                                       gATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTtgtg                                                                                                             <  2:92252/149‑1 (MQ=255)
                                        aTAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGTTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtga                                                                                                            <  1:322319/149‑1 (MQ=255)
                                        aTAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCATTTTAAAGCGTACCATAAAACATAACAAAATAAATGATTTGtga                                                                                                            >  1:116331/1‑149 (MQ=255)
                                         ttaaTATTTATTCGTGGTAATCATAGAGAGTCGCGAGATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtgat                                                                                                           <  2:424743/147‑1 (MQ=255)
                                         tAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGtgat                                                                                                           <  1:1357980/149‑1 (MQ=255)
                                         tAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGAATTGtgat                                                                                                           >  1:1071909/1‑149 (MQ=255)
                                             tatTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTTTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGaca                                                                                                       >  1:15402/1‑149 (MQ=255)
                                             tatTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAATAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATTAAACATAACATAATAAATGATTTGTGATGaca                                                                                                       >  1:1231044/1‑149 (MQ=255)
                                                  aTTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGa                                                                                                  <  2:474987/149‑1 (MQ=255)
                                                       aggTAATCAGAGAGAGTCGCGAAATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGattatt                                                                                             <  2:1522611/148‑1 (MQ=255)
                                                         gTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGc                                                                                           <  2:682720/149‑1 (MQ=255)
                                                               agagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTa                                                                                     >  1:922498/1‑149 (MQ=255)
                                                                gagagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTAc                                                                                    <  1:694931/149‑1 (MQ=255)
                                                                 agagagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATTAATGATTTGTGATGACACGGGATTATTGCGGTTTACt                                                                                   >  1:276148/1‑149 (MQ=255)
                                                                   agagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACttt                                                                                 <  2:1312847/149‑1 (MQ=255)
                                                                   agagTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACttt                                                                                 <  2:174377/149‑1 (MQ=255)
                                                                      gTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAg                                                                              <  1:98245/149‑1 (MQ=255)
                                                                         gcgACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGaa                                                                           <  2:600647/149‑1 (MQ=255)
                                                                              aTTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATaa                                                                      >  1:673177/1‑149 (MQ=255)
                                                                                 tgtgATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAAt                                                                   <  2:717720/149‑1 (MQ=255)
                                                                                  gtgATGTTATTCTTACAACTGTAAGAAGCGCCGGTGAATTACAAGCCAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATg                                                                  <  2:1007356/149‑1 (MQ=255)
                                                                                   tgATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACAAGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGc                                                                 >  2:1177721/1‑149 (MQ=255)
                                                                                    gATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCg                                                                >  1:1433658/1‑149 (MQ=255)
                                                                                     aTGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGa                                                               <  1:257454/149‑1 (MQ=255)
                                                                                       gTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGt                                                             >  2:1246195/1‑149 (MQ=255)
                                                                                        ttATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACCCGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGtt                                                            >  2:1052079/1‑149 (MQ=255)
                                                                                           ttCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtat                                                         <  1:1024286/149‑1 (MQ=255)
                                                                                           ttCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtat                                                         <  1:391756/149‑1 (MQ=255)
                                                                                            tCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTtata                                                        <  1:876218/149‑1 (MQ=255)
                                                                                             cTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGTTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAg                                                       <  2:1370251/149‑1 (MQ=255)
                                                                                             attACAACTGTAAGAAGCGCCGGTGATTTACACGGAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAg                                                       <  2:119188/148‑1 (MQ=255)
                                                                                              ttACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGc                                                      >  1:1056239/1‑149 (MQ=255)
                                                                                                  aaCTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATATGTGATGACACGGGATTATTGCGTTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCAcag                                                  >  1:297468/1‑147 (MQ=255)
                                                                                                   aCTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACcgc                                                 <  2:1473104/149‑1 (MQ=255)
                                                                                                     tGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGa                                               >  1:174873/1‑149 (MQ=255)
                                                                                                     tGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGa                                               >  1:902028/1‑149 (MQ=255)
                                                                                                       tAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAgc                                             >  2:1094514/1‑149 (MQ=255)
                                                                                                            agCGCCGGTGATTAACAAGCAAGGGTATGATTACGCTTTTTAAAGAGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCaa                                        <  1:463100/149‑1 (MQ=255)
                                                                                                            agCGCAGGTGATTTACAAGCAAGGGTTTTATTACGCTTTTTAAAGCGTACGATAAAAGATAACATAATAAATGATTTGTGATTACACGGGATTATTGCGGTTTACTTTGAGGAATCTAAAATGCGAGTTTATAGCACCGCGAGCGCCaa                                        <  2:875926/149‑1 (MQ=255)
                                                                                                                 cGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTTTAGCACCGCGAGCGCCAaataat                                   <  2:61835/149‑1 (MQ=255)
                                                                                                                 cGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAaataat                                   >  2:653627/1‑149 (MQ=255)
                                                                                                                 cGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAaataat                                   <  2:52982/149‑1 (MQ=255)
                                                                                                                  ggTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAAtt                                  >  2:808289/1‑149 (MQ=255)
                                                                                                                  ggTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAACGCGAGTTTATAGCACCGCGAGCGCCAAATAAtt                                  >  1:590397/1‑149 (MQ=255)
                                                                                                                    tGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAAtttt                                <  2:1104319/149‑1 (MQ=255)
                                                                                                                     gATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTa                               <  2:1338943/149‑1 (MQ=255)
                                                                                                                     gATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTa                               >  1:617263/1‑149 (MQ=255)
                                                                                                                        ttACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATTATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGa                            <  2:973218/149‑1 (MQ=255)
                                                                                                                           caagcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACca                         >  2:502032/1‑149 (MQ=255)
                                                                                                                           caagcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACca                         <  1:486617/149‑1 (MQ=255)
                                                                                                                              gcaagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACaa                      >  1:1485321/1‑149 (MQ=255)
                                                                                                                               caagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAAc                     <  2:94787/149‑1 (MQ=255)
                                                                                                                               caagGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAAc                     <  2:1231044/149‑1 (MQ=255)
                                                                                                                                    gTATGATTACGCTTTTTATAGCGTACCATAAAACATAACATAATAACTGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTc                <  2:300834/149‑1 (MQ=255)
                                                                                                                                    gTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTc                >  1:756153/1‑149 (MQ=255)
                                                                                                                                    gTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTc                >  2:406696/1‑149 (MQ=255)
                                                                                                                                       tGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATTCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTa             >  2:1548807/1‑149 (MQ=255)
                                                                                                                                        tggtACGCTTTAAAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTAt            >  1:1128260/4‑149 (MQ=255)
                                                                                                                                          ttACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATtg          <  2:1306169/149‑1 (MQ=255)
                                                                                                                                             cGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTgg       >  2:1210681/1‑149 (MQ=255)
                                                                                                                                             cGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTgg       <  1:1373590/149‑1 (MQ=255)
                                                                                                                                              gCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACAACACCATGTCGTATTGTggg      >  2:246794/1‑148 (MQ=255)
                                                                                                                                              gCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACAACAAACTTTCGTATTGTGGt      >  2:1419066/1‑149 (MQ=255)
                                                                                                                                               cTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTc     <  1:212619/149‑1 (MQ=255)
                                                                                                                                                tttttAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCa    >  1:1144088/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTTCTGCATGGTAAGTAGTGAGGACGAAAAGAAGAATATGATAAATATTGATTCGTGGTAATCAGAGAGAGTCGCGACATTTGTGATGTTATTCTTACAACTGTAAGAAGCGCCGGTGATTTACAAGCAAGGGTATGATTACGCTTTTTAAAGCGTACCATAAAACATAACATAATAAATGATTTGTGATGACACGGGATTATTGCGGTTTACTTTGAGGAATATAAAATGCGAGTTTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATA  >  NC_000913/2493415‑2493709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: