| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,667,359 | A→T | 6.9% | L163* (TTG→TAG) | hcaT ← | putative 3‑phenylpropionic transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,667,359 | 0 | A | T | 6.9% | 157.8 / 3.5 | 58 | L163* (TTG→TAG) | hcaT | putative 3‑phenylpropionic transporter |
| Reads supporting (aligned to +/- strand): ref base A (24/30); new base T (2/2); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01 | |||||||||||
ACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCAT > NC_000913/2667211‑2667503 | acaaacGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCa > 2:1001208/1‑149 (MQ=255) aaacGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAAc > 2:31281/1‑149 (MQ=255) aacGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGAGGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACa < 1:1016003/149‑1 (MQ=255) cAGAAAGCGCCAGTTCTGGCGAACCAGCGCCCACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGa > 1:238649/1‑149 (MQ=255) aaGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCAc < 1:26479/149‑1 (MQ=255) aaGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCAc < 1:1506297/149‑1 (MQ=255) cgcgcCACGTCTGGCTAACCAGCGCCTACCAGGCAGACCACCCGGTGCTCTCCTTCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCAcc < 2:999783/148‑1 (MQ=255) aGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCATCGTCAACAGCGCGAGGATCAcc < 1:61182/149‑1 (MQ=255) cAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATa > 2:1066325/1‑149 (MQ=255) cAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATa < 2:515596/149‑1 (MQ=255) cGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACAt > 1:694291/1‑149 (MQ=255) cAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGa > 1:931255/1‑149 (MQ=255) cAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCTACAGCGCGAGGATCAccc < 2:1047/125‑1 (MQ=255) cAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCTACAGCGCGAGGATCAccc > 1:846912/1‑125 (MQ=255) cAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCTACAGCGCGAGGATCAccc > 1:1047/1‑125 (MQ=255) cAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCTACAGCGCGAGGATCAccc < 2:846912/125‑1 (MQ=255) cAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGtt < 1:55217/149‑1 (MQ=255) ccACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCCCCCGATAATCAAACATAGTGACCAGTTTGc > 1:1385771/1‑149 (MQ=255) ccACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGc < 1:1001208/149‑1 (MQ=255) ccACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGc < 1:1066325/149‑1 (MQ=255) gACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAg > 1:851551/1‑149 (MQ=255) gTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGcc > 1:1570375/1‑149 (MQ=255) ctctCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAAt < 2:238649/149‑1 (MQ=255) ctcCTGCTGGCGACTTGCCCCTTGTGGCGGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGa < 2:371067/149‑1 (MQ=255) tgctgGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACaaa > 2:875870/1‑149 (MQ=255) cGACTTGCCCCTTGTGGCTGATTCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCACGTCAGCGCCGAGCCAATGACAAACGCCAc < 2:1116438/149‑1 (MQ=255) tGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGc < 1:1263731/149‑1 (MQ=255) cccTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGcccc > 2:953562/1‑149 (MQ=255) cTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCcac > 1:512161/1‑149 (MQ=255) ggCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGCGCCAATGACAAACGCCACCGAGCCCCACAGTCg > 1:795814/1‑149 (MQ=255) gCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCCCCGAGCCCCACAGTCGc > 1:1499711/1‑149 (MQ=255) cTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCa > 2:1278274/1‑149 (MQ=255) tGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCAc < 2:1528096/149‑1 (MQ=255) gAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACt < 2:339290/149‑1 (MQ=255) aaTCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCCATAATCAAACAAAGTGACCACTTTCCCCGTCAGCTCCGACCCAATGACAAACGCCACCGAGCCCCACAGTCGCCCtt > 1:1430230/1‑149 (MQ=255) aTCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCa > 2:1056953/1‑91 (MQ=255) aTCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCa < 1:1056953/91‑1 (MQ=255) tcgtcgGACGGATGAGACAGCCGAGCAGCATGGATGCCACGCACAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTg < 2:1018386/149‑1 (MQ=255) gaTGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGTATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCaa < 2:927170/149‑1 (MQ=255) aTGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATTATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCATTGACAAACGCCACCGAGCCCCACAGTCGCACTATGCCGTAATCAAg > 2:281298/1‑149 (MQ=255) gaAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCggg > 1:664021/1‑149 (MQ=255) aaaGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGa > 2:1467775/1‑149 (MQ=255) cagcaTGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCtttt < 2:1499007/149‑1 (MQ=255) tGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCAc < 1:1403056/149‑1 (MQ=255) tGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCAc < 1:875870/149‑1 (MQ=255) tGGATGCCACGCCCAACGTCAAAAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCAc < 1:1063345/149‑1 (MQ=255) aTGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTa > 1:525826/1‑149 (MQ=255) aTGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTa > 1:419638/1‑149 (MQ=255) ccACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTc > 2:439454/1‑149 (MQ=255) ccACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTc < 2:694291/149‑1 (MQ=255) aCGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTCGc > 1:809047/1‑149 (MQ=255) gCCCAACGTGAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACATACGCCACCGAGCCACTCAGTCGCTCTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTTTTCGCCa < 1:578615/149‑1 (MQ=255) cccAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAg < 2:525826/149‑1 (MQ=255) cccAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAg < 2:664021/149‑1 (MQ=255) ccATCGGCAACATCGCGAGGATCACACGATAATCAAACATAGTGACCAGTTTGCCCGTAATCTCTGAGACAATGACAAACGCCACCGAGCCGCACAGTCGCACTTTGCCGTAATCAAGCGGGAATTGCTTTTGCCACGTATTCGCCAGt < 2:259602/149‑1 (MQ=255) ccAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATATTGACCAGTTTGCCCGTCAGCGCCTAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTTATCAAGCGGGAATTGCTTTTGCCACGTATTCGCCAGt < 2:358364/149‑1 (MQ=255) ccAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACACACGCCCCCGAGCCCCACAGTCGCACTTTGCGGTAATCAAGCGGGAACGGCTTTTGCCACTTTGTCGCCAGt > 2:314896/1‑149 (MQ=255) cAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGGCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAGTg < 2:851551/149‑1 (MQ=255) cGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCAt < 1:31281/149‑1 (MQ=255) | ACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGGTGCTCTCCTGCTGGCGACTTGCCCCTTGTGGCTGAATCGTCGGACGGATGAGAAAGCCGAGCAGCATGGATGCCACGCCCAACGTCAACAGCGCGAGGATCACCCGATAATCAAACATAGTGACCAGTTTGCCCGTCAGCGCCGAGCCAATGACAAACGCCACCGAGCCCCACAGTCGCACTTTGCCGTAATCAAGCGGGAACTGCTTTTGCCACGTATTCGCCAGTGCAT > NC_000913/2667211‑2667503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |