Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,687,214 T→G 7.0% V65V (GTA→GTC glnB ← regulatory protein P‑II for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,687,2140TG 7.0% 235.6 / 7.7 82V65V (GTA→GTCglnBregulatory protein P‑II for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (41/35);  new base G (2/4);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

CGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGCTTCGCGGA  >  NC_000913/2687066‑2687362
                                                                                                                                                    |                                                                                                                                                    
cgGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGt                                                                                                                                                      <  2:74115/149‑1 (MQ=255)
  gTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTAc                                                                                                                                                    <  2:1024300/149‑1 (MQ=255)
   tttAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACg                                                                                                                                                   >  2:1228053/1‑149 (MQ=255)
      aaaTTGCCGCGTCGTCCTCGTAACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGGGAAGAATGGGAGCGACACAGGTATCGACAATGTCGTCGCGTAAGACa                                                                                                                                                >  2:242576/1‑149 (MQ=255)
          tGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAAtct                                                                                                                                            <  1:179496/149‑1 (MQ=255)
          tGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAAtct                                                                                                                                            <  2:1587340/149‑1 (MQ=255)
          tGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTACCGGACGACattgt                                                                                                                                            <  1:1166806/149‑5 (MQ=255)
          tGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTACCGGACGACattgt                                                                                                                                            <  2:1527102/149‑5 (MQ=255)
          tGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTACCGGACGACattgt                                                                                                                                            <  1:1532341/149‑5 (MQ=255)
           gCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTACCGGACGACattgtc                                                                                                                                           <  2:1554421/149‑6 (MQ=255)
            ccGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCa                                                                                                                                          >  1:669593/1‑149 (MQ=255)
             cgcgTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCaa                                                                                                                                         <  2:1447788/149‑1 (MQ=255)
             cgcgTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCaa                                                                                                                                         <  2:1454245/149‑1 (MQ=255)
               cgtcgtCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAAtt                                                                                                                                       <  1:1536819/149‑1 (MQ=255)
                 gcgtcCTCTTCACCGGTACGGATGCGAATGACCCGAGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAAtttt                                                                                                                                     <  2:1091110/148‑1 (MQ=255)
                   gtcCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCa                                                                                                                                   >  1:1354752/1‑149 (MQ=255)
                   gtcCTCATCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCa                                                                                                                                   <  1:851385/149‑1 (MQ=255)
                    tcCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCAc                                                                                                                                  >  1:487814/1‑149 (MQ=255)
                      ctctTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACtt                                                                                                                                >  1:1202160/1‑149 (MQ=255)
                      ctctTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACtt                                                                                                                                >  1:368045/1‑149 (MQ=255)
                       tctTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACttt                                                                                                                               <  2:1202160/149‑1 (MQ=255)
                            aCCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCa                                                                                                                          >  1:152922/1‑149 (MQ=255)
                               ggTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGaa                                                                                                                       >  2:341194/1‑149 (MQ=255)
                                     gATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCGCTTTGGGCAGAAAATccc                                                                                                                 >  2:595404/1‑148 (MQ=255)
                                     gATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATcca                                                                                                                 <  2:206972/149‑1 (MQ=255)
                                     gATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATcca                                                                                                                 >  1:471958/1‑149 (MQ=255)
                                      ttGCGAATGACCCGTGCCACGTCAAAGACGAGGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATccac                                                                                                                <  2:1222359/148‑1 (MQ=255)
                                      aTGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATccac                                                                                                                <  2:471958/149‑1 (MQ=255)
                                         cGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCat                                                                                                             <  2:1354752/149‑1 (MQ=255)
                                          gAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAACTCCACCata                                                                                                            >  1:626661/1‑149 (MQ=255)
                                            aTGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCAtata                                                                                                          >  2:1097970/1‑149 (MQ=255)
                                             tGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATAc                                                                                                         <  2:231824/149‑1 (MQ=255)
                                                 ccGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGGACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTccg                                                                                                     >  1:777688/1‑149 (MQ=255)
                                                       cACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTccgcgccgc                                                                                               <  2:596528/149‑1 (MQ=255)
                                                         cGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGTGCCGCgg                                                                                             >  2:246150/1‑149 (MQ=255)
                                                         cGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCgg                                                                                             <  1:897247/149‑1 (MQ=255)
                                                              aaGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAg                                                                                        >  2:690239/1‑149 (MQ=255)
                                                                 aagAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGCCAATCTCAATTTTCACTTTAGGCAGAAAATCCACCAAATACACCGCGCCGCGGTACAGCTc                                                                                     >  2:164094/3‑149 (MQ=255)
                                                                 aCGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTc                                                                                     >  1:537586/1‑149 (MQ=255)
                                                                   gAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCgg                                                                                   >  2:1164656/1‑149 (MQ=255)
                                                                   gAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCgg                                                                                   >  1:1419744/1‑149 (MQ=255)
                                                                   gAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCgg                                                                                   >  1:606344/1‑149 (MQ=255)
                                                                        ttttACCGTCACCGATTTTGCCGGTTTGCGCCGGGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATgg                                                                              <  2:967360/149‑1 (MQ=255)
                                                                         tttACCGTCACCGATTTTGCCGGTTTGCGCCGTGTGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGc                                                                             >  1:637427/1‑149 (MQ=255)
                                                                            aCCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCtt                                                                          <  2:1334911/149‑1 (MQ=255)
                                                                              cGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTc                                                                        <  1:58667/149‑1 (MQ=255)
                                                                                  aCCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAGATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGc                                                                    >  2:275379/1‑149 (MQ=255)
                                                                                  aCCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGc                                                                    >  2:795641/1‑149 (MQ=255)
                                                                                  aCCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGc                                                                    <  2:1236485/149‑1 (MQ=255)
                                                                                    cGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGa                                                                  >  1:1320810/1‑149 (MQ=255)
                                                                                             cGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCt                                                         >  1:1262995/1‑149 (MQ=255)
                                                                                              ggTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCtt                                                        <  1:981226/149‑1 (MQ=255)
                                                                                               gTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACATTCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACCGCTCGGTATGGCCTATCTGGCGACCAAAGCCttt                                                       >  1:646382/1‑149 (MQ=255)
                                                                                                tttGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTc                                                      <  2:181358/149‑1 (MQ=255)
                                                                                                 ttGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCa                                                     <  2:617149/149‑1 (MQ=255)
                                                                                                    cgcCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACtt                                                  >  2:1532114/1‑149 (MQ=255)
                                                                                                    cgcCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACtt                                                  <  2:609191/149‑1 (MQ=255)
                                                                                                     gcCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTc                                                 >  1:1461775/1‑149 (MQ=255)
                                                                                                       cGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCTGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCgg                                               <  2:669593/149‑1 (MQ=255)
                                                                                                        gTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGt                                              >  1:420290/1‑149 (MQ=255)
                                                                                                        gTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATAAACTCCGCTCCGCGGTACAGCTCGTTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTAGGt                                              >  1:147939/1‑149 (MQ=255)
                                                                                                         tGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCggca                                             >  2:534108/1‑147 (MQ=255)
                                                                                                            gAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCGTTCACTTCGGTCAcc                                          >  1:275285/1‑149 (MQ=255)
                                                                                                             aataatGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCg                                         <  2:802623/149‑1 (MQ=255)
                                                                                                             aataatGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGGCACCg                                         <  2:1170570/149‑1 (MQ=255)
                                                                                                                aatGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCa                                      >  1:135173/1‑149 (MQ=255)
                                                                                                                   ggTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGc                                   >  1:903636/1‑149 (MQ=255)
                                                                                                                      aTCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCgg                                <  2:1419744/149‑1 (MQ=255)
                                                                                                                      aTCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCgg                                <  2:375422/149‑1 (MQ=255)
                                                                                                                         gACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTaa                             >  1:601585/1‑149 (MQ=255)
                                                                                                                          acacAGGTATCGACAATGTCGTCCGGTACGACAATCACAATTTTCACTATCGGCAGAAAAACCACCATATACTCCGCGCCGCTGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTATCACTTCGGTCACCGTCATGCCGGAAAt                            >  1:280485/1‑149 (MQ=255)
                                                                                                                           cacaGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATa                           <  2:942043/149‑1 (MQ=255)
                                                                                                                             caGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATAcc                         <  1:307271/149‑1 (MQ=255)
                                                                                                                                gTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGAc                      <  1:690239/149‑1 (MQ=255)
                                                                                                                                  aTCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACtt                    <  2:152922/149‑1 (MQ=255)
                                                                                                                                   tCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGCCCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTTATACAGACTTc                   >  2:122948/1‑149 (MQ=255)
                                                                                                                                   tCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTc                   >  2:1310328/1‑149 (MQ=255)
                                                                                                                                   tCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTc                   >  2:1144660/1‑149 (MQ=255)
                                                                                                                                      aCAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAACGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGc                >  1:631212/1‑149 (MQ=255)
                                                                                                                                       gattGTCGTACCGGACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGcc               >  1:20972/4‑149 (MQ=255)
                                                                                                                                            tcgtcCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGc          >  1:467868/1‑149 (MQ=255)
                                                                                                                                               tcCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGCTTc       <  1:189843/149‑1 (MQ=255)
                                                                                                                                                 cGGTACGACAATCTCAAATTTCACTTTCCGCAGAAAATCCACCATATACTCCGCGCCGCGGTCCAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGCTTcgc     <  2:247427/149‑1 (MQ=255)
                                                                                                                                                    tACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGCTTCGCGGa  <  2:1230687/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGGTATCGACAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGCTTCGCGGA  >  NC_000913/2687066‑2687362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: