Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,691,173 A→T 8.1% noncoding (168/184 nt) glmY ← sRNA activator of glmS mRNA, glmZ processing antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,691,1730AT 8.1% 312.9 / 14.5 111noncoding (168/184 nt)glmYsRNA activator of glmS mRNA, glmZ processing antagonist
Reads supporting (aligned to +/- strand):  ref base A (58/43);  new base T (3/6);  total (61/50)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

Only 100 of 112 total aligned reads displayed.

GCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATA  >  NC_000913/2691026‑2691321
                                                                                                                                                   |                                                                                                                                                    
gcagcaGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTAc                                                                                                                                                     >  2:1581370/1‑149 (MQ=255)
gaagaagAATCAGAAAAAATGCCAGCATAACCAGTTGTAGTAATGAGCGGGGAAAAACGTGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTAc                                                                                                                                                     <  1:1276757/144‑1 (MQ=255)
  agcagAATCAGCAAAAATGCCAGCATTACCAGTTGACGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGATTAGCCTGATCCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTATTAcac                                                                                                                                                   >  1:68441/1‑147 (MQ=255)
    cagAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCgg                                                                                                                                                 <  1:1034591/149‑1 (MQ=255)
     agAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGc                                                                                                                                                <  1:1506995/149‑1 (MQ=255)
      gAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCt                                                                                                                                               <  2:161491/149‑1 (MQ=255)
         tCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGCTTTg                                                                                                                                            <  1:1468194/149‑1 (MQ=255)
          cAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGCTTTGt                                                                                                                                           <  2:1135544/149‑1 (MQ=255)
           aGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGCTTTGtt                                                                                                                                          <  2:1254171/149‑1 (MQ=255)
              aaaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAACCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATg                                                                                                                                       <  1:192813/149‑1 (MQ=255)
              aaaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATTCCCGGCTTTGttgta                                                                                                                                       <  1:1278323/149‑4 (MQ=255)
              aaaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATg                                                                                                                                       <  1:187700/149‑1 (MQ=255)
                aaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGCTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATATAACAAAGCCGGGTAATTCCCGGCTTTGttgtatc                                                                                                                                     <  1:1163623/149‑6 (MQ=39)
                    gCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGGTACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATaa                                                                                                                                 >  1:1103454/1‑149 (MQ=255)
                     ccAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAg                                                                                                                                <  2:384928/149‑1 (MQ=255)
                      cAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACTACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAgg                                                                                                                               >  1:1004907/1‑149 (MQ=255)
                      cAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATaatg                                                                                                                               >  1:607411/1‑147 (MQ=255)
                       aGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATAGAATAAGGc                                                                                                                              >  1:4044/1‑149 (MQ=255)
                           ttACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACTCGAATAGACTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTg                                                                                                                          >  1:266878/1‑149 (MQ=255)
                           ttACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTg                                                                                                                          <  2:354980/149‑1 (MQ=255)
                            tACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGc                                                                                                                         <  2:1094326/149‑1 (MQ=255)
                            tACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGc                                                                                                                         <  2:469196/149‑1 (MQ=255)
                              ccAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCt                                                                                                                       >  1:1246153/1‑149 (MQ=255)
                               cAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTa                                                                                                                      >  1:767356/1‑149 (MQ=255)
                                  ttGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACt                                                                                                                   <  2:1077674/149‑1 (MQ=255)
                                  ttGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACt                                                                                                                   >  2:7250/1‑149 (MQ=255)
                                         aaTGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGtt                                                                                                            <  2:1275326/149‑1 (MQ=255)
                                          ctGAGCGGGGAAACACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGttt                                                                                                           <  1:500175/148‑1 (MQ=255)
                                             cgccgggCAAAACGGGCCAGCGGTTCAACGGGGTACCCTCGTCATTCCCGAAAAGCCGGCTGGCAACCGCCGGGAAGGTCAGCTACAACAAACCCGGGAAATACCCGGGGTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGc                                                                                                        <  1:832697/145‑1 (MQ=255)
                                             aGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGc                                                                                                        <  2:778041/149‑1 (MQ=255)
                                               cGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACGCTCGTCTGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTGCAGATACAACATAGCCGGGAATTACCCGGCTTTGTTATGTAATATGGGGGTGCCTAACTCGACGTTTCGccc                                                                                                      <  1:1539058/149‑1 (MQ=255)
                                                ggggAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCg                                                                                                     <  1:393072/149‑1 (MQ=255)
                                                   gAAAAACGGTCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATg                                                                                                  <  2:1077338/149‑1 (MQ=255)
                                                      aaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGtt                                                                                               <  2:1447751/149‑1 (MQ=255)
                                                             ccAGCGTTACAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAg                                                                                        >  1:837070/1‑149 (MQ=255)
                                                               aGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCt                                                                                      >  2:175290/1‑149 (MQ=255)
                                                                  gTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACg                                                                                   >  2:790254/1‑149 (MQ=255)
                                                                   tttCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGc                                                                                  >  1:1318568/1‑149 (MQ=255)
                                                                         ggTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGatat                                                                            >  2:733822/1‑149 (MQ=255)
                                                                           ggtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGGGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCa                                                                          <  1:518321/148‑1 (MQ=255)
                                                                            gtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCTCCCGATGGTTGATATAGCTACGCTGATATCAg                                                                         >  2:405738/1‑149 (MQ=255)
                                                                            gtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAg                                                                         >  1:1427151/1‑149 (MQ=255)
                                                                             ttACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGa                                                                        >  1:1337415/1‑149 (MQ=255)
                                                                             ttACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGa                                                                        >  2:1279429/1‑149 (MQ=255)
                                                                             ttACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGa                                                                        >  2:392683/1‑149 (MQ=255)
                                                                                 tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                                    >  2:1545123/1‑149 (MQ=255)
                                                                                 tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                                    >  2:1408673/1‑149 (MQ=255)
                                                                                 tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                                    >  1:687671/1‑149 (MQ=255)
                                                                                 tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                                    >  1:629817/1‑149 (MQ=255)
                                                                                  ctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTg                                                                   <  1:1044486/149‑1 (MQ=255)
                                                                                  ctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTg                                                                   <  1:1573738/149‑1 (MQ=255)
                                                                                  ctcGTCAGACGCGAATAGCCTGATGATAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACACGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTg                                                                   <  2:1588815/149‑1 (MQ=255)
                                                                                   tcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAACCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCATAACTCGACGTTTCTCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTgg                                                                  >  2:74947/1‑149 (MQ=255)
                                                                                     gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAc                                                                >  1:850922/1‑149 (MQ=255)
                                                                                     gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAc                                                                >  1:516301/1‑149 (MQ=255)
                                                                                     gTCAGACGCGAATAGCCTGATGCTAACCGACGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGAGGGTTGATATAGCTACGCTGATATCAGAAGTTGGAc                                                                <  1:829707/149‑1 (MQ=255)
                                                                                      tCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACg                                                               >  2:1284495/1‑149 (MQ=255)
                                                                                      tCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACg                                                               >  1:374635/1‑149 (MQ=255)
                                                                                            gcgAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACTACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggcaggc                                                         <  2:1004907/149‑1 (MQ=255)
                                                                                            gcgAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggcaggc                                                         >  1:970790/1‑149 (MQ=255)
                                                                                              gAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCAc                                                       <  1:1581370/149‑1 (MQ=255)
                                                                                              gAATAGACTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGTAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTACCTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGCCGGCAGGCAc                                                       >  2:1299434/1‑149 (MQ=255)
                                                                                               aaTAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCAcc                                                      <  1:790254/149‑1 (MQ=255)
                                                                                                   gCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgt                                                  >  1:965048/1‑149 (MQ=255)
                                                                                                   gCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGATATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgt                                                  >  1:733344/1‑149 (MQ=255)
                                                                                                    ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                                 >  2:399768/1‑149 (MQ=255)
                                                                                                    ccTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                                 >  2:541406/1‑149 (MQ=255)
                                                                                                      tGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTtgttgt                                               <  2:1251718/149‑1 (MQ=255)
                                                                                                       gATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTtgtg                                              <  2:266878/149‑1 (MQ=255)
                                                                                                        aTGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCTGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATTTAGCTACGCTGATATCAGAAGTTGGACTGCAGGCACCTTGTTGTGc                                             <  1:786159/149‑1 (MQ=255)
                                                                                                         tGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCg                                            >  2:803889/1‑149 (MQ=255)
                                                                                                           cTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTc                                          >  1:1006665/1‑149 (MQ=255)
                                                                                                            tAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCa                                         >  1:1151011/1‑149 (MQ=255)
                                                                                                              aacGAGGGGAAGTTCAGATACAACAAAGCCGCGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGGTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAAGGCAGGCACCTTGTTGTGCGTCAtt                                       <  1:392971/147‑1 (MQ=255)
                                                                                                               ccGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTc                                      <  1:1065948/149‑1 (MQ=255)
                                                                                                                    gggAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTAtt                                 >  1:1487534/1‑149 (MQ=255)
                                                                                                                      gAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTAtttt                               <  1:175290/149‑1 (MQ=255)
                                                                                                                      gAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTAtttt                               >  1:560084/1‑149 (MQ=255)
                                                                                                                      gAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTAtttt                               >  1:76679/1‑149 (MQ=255)
                                                                                                                       aaGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTa                              <  2:516301/149‑1 (MQ=255)
                                                                                                                         gTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCCCCTTGTAGTGCGTCATTCGTATTTTATg                            >  2:649266/1‑149 (MQ=255)
                                                                                                                         gTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATg                            >  1:1432212/1‑149 (MQ=255)
                                                                                                                         gTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATg                            >  1:1583345/1‑149 (MQ=255)
                                                                                                                          ttCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCTTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGt                           <  2:619883/149‑1 (MQ=255)
                                                                                                                          ttCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGt                           >  1:450208/1‑149 (MQ=255)
                                                                                                                          ttCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGt                           <  1:360690/149‑1 (MQ=255)
                                                                                                                                tACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACg                     >  2:1281903/1‑149 (MQ=255)
                                                                                                                                 ataacaaAGCCGGGTAATTCCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGt                    >  2:841826/3‑149 (MQ=255)
                                                                                                                                   aacaaAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTcc                  >  2:8041/1‑149 (MQ=255)
                                                                                                                                    acaaAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTccc                 >  2:1382895/1‑149 (MQ=255)
                                                                                                                                     caaAGCCGGGTAATTCCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCg                >  1:1292921/1‑149 (MQ=255)
                                                                                                                                     caaAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCg                >  1:1584984/1‑149 (MQ=255)
                                                                                                                                     caaAGCCGGGAATTACCCGGCGTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTccag                >  1:113446/1‑147 (MQ=255)
                                                                                                                                         gCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGTCGGTTCGCCCGATGCTTGATATAGCTACGCTGATATCAGAAGTTGGCCGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAgg            <  1:971678/149‑1 (MQ=255)
                                                                                                                                            gggAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGc         <  2:1246153/149‑1 (MQ=255)
                                                                                                                                            gggAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGc         >  1:99734/1‑149 (MQ=255)
                                                                                                                                             ggAATTCCCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTCGCCGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCt        <  1:288179/149‑1 (MQ=255)
                                                                                                                                               aaTTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGa      <  2:1309932/149‑1 (MQ=255)
                                                                                                                                                  tACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACAt   <  1:392683/149‑1 (MQ=255)
                                                                                                                                                   aCCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATa  >  2:429906/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAGCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGAATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGGCTGACATA  >  NC_000913/2691026‑2691321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: