Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,714,415 C→T 7.1% intergenic (+185/+24) srmB → / ← yfiE ATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,714,4150CT 7.1% 308.0 / 7.9 98intergenic (+185/+24)srmB/yfiEATP‑dependent RNA helicase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (49/42);  new base T (4/3);  total (53/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

CAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATT  >  NC_000913/2714270‑2714563
                                                                                                                                                 |                                                                                                                                                    
cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCgg                                                                                                                                                   <  1:632698/149‑1 (MQ=255)
   aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGtt                                                                                                                                                <  1:1228587/149‑1 (MQ=255)
    cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGttt                                                                                                                                               <  2:181836/149‑1 (MQ=255)
      cAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTaa                                                                                                                                             >  1:671967/1‑149 (MQ=255)
              ccTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACa                                                                                                                                     <  1:577495/149‑1 (MQ=255)
               cTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCTTCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACat                                                                                                                                    >  1:122521/1‑149 (MQ=255)
               cTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGTCGGGATTAAACCCGGCat                                                                                                                                    <  1:901128/149‑1 (MQ=255)
                 gATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGCTTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTACTCCCGACatta                                                                                                                                  >  1:661908/1‑149 (MQ=255)
                 gATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACatta                                                                                                                                  >  2:230600/1‑149 (MQ=255)
                   tGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGTCGGGATTAAACCCGGCatttaa                                                                                                                                <  1:110200/149‑4 (MQ=18)
                   tGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTAGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGTCGGGATTAAACCCGGCatttaa                                                                                                                                <  1:557190/149‑4 (MQ=18)
                    gcgcTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTAttt                                                                                                                               <  1:54025/149‑1 (MQ=255)
                      gcTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTAttttt                                                                                                                             >  2:377279/1‑149 (MQ=255)
                      gcTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTAttttt                                                                                                                             <  1:243444/149‑1 (MQ=255)
                      gcTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACACTAttttt                                                                                                                             >  2:64747/1‑149 (MQ=255)
                       cTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTa                                                                                                                            <  1:1504619/149‑1 (MQ=255)
                       cTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTa                                                                                                                            <  2:441855/149‑1 (MQ=255)
                       cTACGCTTATCAGGCCGACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTa                                                                                                                            <  1:132261/149‑1 (MQ=255)
                        tACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTAt                                                                                                                           >  2:543851/1‑149 (MQ=255)
                         aCGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATc                                                                                                                          <  2:631636/149‑1 (MQ=255)
                          cGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATcc                                                                                                                         <  2:1032287/149‑1 (MQ=255)
                           gCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCa                                                                                                                        >  2:763287/1‑149 (MQ=255)
                            cTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAg                                                                                                                       >  1:210168/1‑149 (MQ=255)
                               aTCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCca                                                                                                                    >  2:1374458/1‑149 (MQ=255)
                                    gCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACaaaa                                                                                                               <  2:1513387/149‑1 (MQ=255)
                                    gCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACaaaa                                                                                                               >  1:1576020/1‑149 (MQ=255)
                                      cTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAAct                                                                                                             >  2:1579947/1‑149 (MQ=255)
                                          gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAActctct                                                                                                         >  1:320545/1‑149 (MQ=255)
                                           tGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAAGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCtt                                                                                                        <  1:1531759/149‑1 (MQ=255)
                                           gggCTCATGCAATATATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTAAACTGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCtt                                                                                                        <  1:200802/148‑1 (MQ=255)
                                            ggCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTc                                                                                                       <  2:1313853/149‑1 (MQ=255)
                                            ggCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTc                                                                                                       <  1:430407/149‑1 (MQ=255)
                                            ggCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTc                                                                                                       <  2:682063/149‑1 (MQ=255)
                                             gCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCa                                                                                                      >  2:995232/1‑149 (MQ=255)
                                             gCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCa                                                                                                      >  2:536273/1‑149 (MQ=255)
                                             gCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCAACAAAACTCTCTTCa                                                                                                      >  2:992266/1‑149 (MQ=255)
                                                   gCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAacacac                                                                                                >  2:1565173/1‑149 (MQ=255)
                                                     aaTATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTg                                                                                              >  2:289841/1‑149 (MQ=255)
                                                      atatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGa                                                                                             >  2:774539/1‑149 (MQ=255)
                                                      atatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGa                                                                                             >  1:1528154/1‑149 (MQ=255)
                                                          atTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATaa                                                                                         >  2:1185132/1‑149 (MQ=255)
                                                              aaTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAgt                                                                                     <  2:1576201/149‑1 (MQ=255)
                                                                tttGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAgtgt                                                                                   <  1:264180/149‑1 (MQ=255)
                                                                    cACGATCTTGTAGGCCTGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCAt                                                                               >  1:1364548/1‑149 (MQ=255)
                                                                    cACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCAt                                                                               >  2:322665/1‑149 (MQ=255)
                                                                    cACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCAt                                                                               >  2:545804/1‑149 (MQ=255)
                                                                     aCGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAACCTCTCTTCAACACACTGAATAAAAGTGTGCGTc                                                                              >  2:132481/1‑149 (MQ=255)
                                                                     aCGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATc                                                                              >  2:771995/1‑149 (MQ=255)
                                                                     aCGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATc                                                                              >  1:58109/1‑149 (MQ=255)
                                                                         tCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCg                                                                          >  1:687540/1‑149 (MQ=255)
                                                                         tCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCg                                                                          >  2:606279/1‑149 (MQ=255)
                                                                               atgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTaa                                                                    >  2:342258/3‑149 (MQ=39)
                                                                               atgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTaa                                                                    >  2:498528/3‑149 (MQ=39)
                                                                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAAc                                                                   >  2:786673/1‑149 (MQ=255)
                                                                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATaaa                                                                                        <  1:537152/127‑1 (MQ=255)
                                                                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATaaa                                                                                        >  2:537152/1‑127 (MQ=255)
                                                                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAAcc                                                                  >  1:1229778/1‑149 (MQ=255)
                                                                                        aaGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTc                                                           <  1:289841/149‑1 (MQ=255)
                                                                                          ggCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCa                                                         >  2:742496/1‑149 (MQ=255)
                                                                                          ggCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCa                                                         >  2:604635/1‑149 (MQ=255)
                                                                                          ggCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCa                                                         <  1:964225/149‑1 (MQ=255)
                                                                                          ggCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCa                                                         <  1:16390/149‑1 (MQ=255)
                                                                                            tattCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGc                                                       >  2:249854/3‑149 (MQ=255)
                                                                                             gTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCa                                                      >  2:1309559/1‑149 (MQ=255)
                                                                                              ttCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCat                                                     <  1:377279/149‑1 (MQ=255)
                                                                                              ttCACGACGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACACTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCat                                                     <  1:64747/149‑1 (MQ=255)
                                                                                                 aCGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCatgat                                                  >  1:1328333/1‑149 (MQ=255)
                                                                                                  cgccgcATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCatgatg                                                 >  2:64695/1‑149 (MQ=255)
                                                                                                    ccgcATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGAtgtg                                               <  1:1565173/149‑1 (MQ=255)
                                                                                                     cgcATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTgc                                              <  2:1528154/149‑1 (MQ=255)
                                                                                                     cgcATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTgc                                              <  1:771995/149‑1 (MQ=255)
                                                                                                      gcATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTgcg                                             <  1:786673/149‑1 (MQ=255)
                                                                                                        aTCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGca                                           >  1:33391/1‑149 (MQ=255)
                                                                                                         tCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGcac                                          >  1:1529923/1‑149 (MQ=255)
                                                                                                         tCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGcac                                          <  2:305133/149‑1 (MQ=255)
                                                                                                           cGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCCCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACAt                                        <  1:1166491/149‑1 (MQ=255)
                                                                                                            ggCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATg                                       <  1:1374458/149‑1 (MQ=255)
                                                                                                               aTGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGcc                                    <  1:995232/149‑1 (MQ=255)
                                                                                                                 gAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGt                                  <  2:393112/149‑1 (MQ=255)
                                                                                                                  aaTAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCAAGCATGATGTGCGCACATGGCCGTa                                 >  1:397211/1‑149 (MQ=255)
                                                                                                                    tAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAAt                               <  2:122521/149‑1 (MQ=255)
                                                                                                                         cgcACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCt                          >  1:1296269/1‑149 (MQ=255)
                                                                                                                                 gTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGt                  <  2:468214/149‑1 (MQ=255)
                                                                                                                                   cGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTc                <  1:763287/149‑1 (MQ=255)
                                                                                                                                    gACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCGTCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCg               >  1:1256870/1‑149 (MQ=255)
                                                                                                                                    gACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCg               >  1:391121/1‑149 (MQ=255)
                                                                                                                                     aCAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGc              >  2:503528/1‑149 (MQ=255)
                                                                                                                                     aCAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGc              <  1:774539/149‑1 (MQ=255)
                                                                                                                                     aCAAGTTAAATGCCGGGTTTAATCCCGACAGTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCAACGCCGGCCTAACCGCGTTTCCAGCATGATGTGCGCCCAGGGCCGTAATGGTCTGCGCCAGTTCGc              >  2:57355/1‑149 (MQ=255)
                                                                                                                                      taaaaataaTGTCGGGATTAAACCCGGCATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGcc             >  2:11847/8‑149 (MQ=255)
                                                                                                                                      taaaaataaTGTCGGGATTAAACCCGGCATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGcc             >  2:1199047/8‑149 (MQ=255)
                                                                                                                                      taaaaataaTGTCGGGATTAAACCCGGCATTATTTTTATCCAGCCACAAAACTCTCTTCAACACAATGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGcc             >  2:6200/8‑149 (MQ=255)
                                                                                                                                        aGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCaa           >  1:1102655/1‑149 (MQ=255)
                                                                                                                                         aaataaTGTCGGGATTAAACCCGGCATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCaaa          >  1:1335358/5‑149 (MQ=255)
                                                                                                                                          ttAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCaaaa         <  2:210168/149‑1 (MQ=255)
                                                                                                                                           tAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAg        <  1:64695/149‑1 (MQ=255)
                                                                                                                                                gCCGGGTTTATTCCCGCCATTTTTTTTATCCAGCCTCATAACTCTCTGCAACACACTGAATACACGTGTGCTTCGCCGGGCTAACCGCGTTTCCAGCATGATGTGCGCACATGGCCGTAAGGGGCTGCGACTGTTCGCCAAAAGGcaat   <  1:57355/149‑1 (MQ=255)
                                                                                                                                                gCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaat   <  1:606279/149‑1 (MQ=255)
                                                                                                                                                 ccGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGcaatt  >  2:200767/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAATGCCGGGTTTAATCCCGACATTATTTTTATCCAGCCACAAAACTCTCTTCAACACACTGAATAAAAGTGTGCATCGCCGGGCTAACCGCTTTTCCAGCATGATGTGCGCACATGGCCGTAATGGTCTGCGACTGTTCGCCAAAAGGCAATT  >  NC_000913/2714270‑2714563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑