Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,748,848 G→T 6.1% G25G (GGG→GGT)  yfjD → UPF0053 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,748,8480GT 6.1% 359.8 / 7.1 114G25G (GGG→GGT) yfjDUPF0053 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (53/54);  new base T (4/3);  total (57/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 116 total aligned reads displayed.

TCTGACACTGGCCTACTTCGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATA  >  NC_000913/2748702‑2748996
                                                                                                                                                  |                                                                                                                                                    
tCTGACACTGGCCTACTTCGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCAGGGTGGTCATTTCAGCCTATTTTTCCGGGTc                                                                                                                                                    >  2:1364907/1‑149 (MQ=255)
        tGGCCTACTTCGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCg                                                                                                                                            <  2:126124/149‑1 (MQ=255)
        tGGCCTACTTCGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCg                                                                                                                                            <  2:762470/149‑1 (MQ=255)
           ccTACTTCGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACAACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGaa                                                                                                                                         >  2:687456/1‑149 (MQ=255)
            cTACTTCGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAat                                                                                                                                        <  2:709741/149‑1 (MQ=255)
                ttCGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGTTTCGGACCCGGaaaaata                                                                                                                                    <  1:204075/149‑6 (MQ=255)
                 tCGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGTTTCGGACCCGGaaaaatag                                                                                                                                   <  1:349703/149‑7 (MQ=255)
                  cGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGAc                                                                                                                                  <  2:280478/149‑1 (MQ=255)
                      aGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTc                                                                                                                              <  1:322797/149‑1 (MQ=255)
                        ccGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCaa                                                                                                                            >  1:1171279/1‑149 (MQ=255)
                        ccGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCaa                                                                                                                            >  1:1007386/1‑149 (MQ=255)
                            aTTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGc                                                                                                                        <  2:424020/149‑1 (MQ=255)
                            aTTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGc                                                                                                                        >  2:634320/1‑149 (MQ=255)
                             ttGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCt                                                                                                                       <  1:645177/149‑1 (MQ=255)
                               gTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTAt                                                                                                                     >  1:1466668/1‑149 (MQ=255)
                                tCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATc                                                                                                                    >  1:1421767/1‑149 (MQ=255)
                                  cagcagTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGCAACCGGAATGATGACCCTCAACCGCTATCGt                                                                                                                  >  1:1403954/1‑149 (MQ=255)
                                     cagTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTg                                                                                                               <  2:1313371/149‑1 (MQ=255)
                                      agTTACTCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGc                                                                                                              <  2:1512041/149‑1 (MQ=255)
                                        ttAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGa                                                                                                            <  2:1283425/149‑1 (MQ=255)
                                        ttAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGa                                                                                                            <  2:396510/149‑1 (MQ=255)
                                           aTCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACat                                                                                                         <  1:404608/149‑1 (MQ=255)
                                              ttcTAAACCCAGAAAAGGAGTTTTCCCTGGAACACATTTCTACTACTTCGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCATTTTTTTCCGGGTCGGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATg                                                                                                      <  1:45720/147‑1 (MQ=255)
                                              atcTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATg                                                                                                      <  2:813272/147‑1 (MQ=255)
                                              aGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATg                                                                                                      >  2:193585/1‑149 (MQ=255)
                                              aGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATg                                                                                                      <  1:1048549/149‑1 (MQ=255)
                                               gCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATgg                                                                                                     <  1:1366595/149‑1 (MQ=255)
                                               gCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATgg                                                                                                     >  1:1234155/1‑149 (MQ=255)
                                               gCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATgg                                                                                                     >  1:615666/1‑149 (MQ=255)
                                                cTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGc                                                                                                    <  2:354670/149‑1 (MQ=255)
                                                        aGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAgg                                                                                            <  2:144859/149‑1 (MQ=255)
                                                        aGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAgg                                                                                            <  1:416718/149‑1 (MQ=255)
                                                         gAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAggg                                                                                           <  1:1569521/149‑1 (MQ=255)
                                                            aaGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTaa                                                                                        <  1:1399918/149‑1 (MQ=255)
                                                                aGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCCGCGACATATGGCGAAACAGGGTAATCGc                                                                                    >  1:82045/1‑149 (MQ=255)
                                                                 gTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCt                                                                                   >  1:932876/1‑149 (MQ=255)
                                                                  tttCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTc                                                                                  <  1:1525033/149‑1 (MQ=255)
                                                                       ccTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCa                                                                             <  2:932876/149‑1 (MQ=255)
                                                                       ccTGGAACACATTTCTACTACTACGTTGATAATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTCCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGCCATATGGCGACACAGGGTAAACGCTCGGCCa                                                                             >  2:616852/1‑149 (MQ=255)
                                                                        cTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCaa                                                                            >  2:1274625/1‑149 (MQ=255)
                                                                           gAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAAcg                                                                         >  1:385219/1‑149 (MQ=255)
                                                                            aaCACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAAcgc                                                                        >  2:335213/1‑149 (MQ=255)
                                                                              cacaTTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAAcgcgg                                                                      >  2:679898/1‑148 (MQ=255)
                                                                              cacaTTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGt                                                                      <  1:1148885/149‑1 (MQ=255)
                                                                               acaTTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTc                                                                     >  2:414791/1‑149 (MQ=255)
                                                                               acaTTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGC‑CGTACAAACGCATcc                                                                    >  2:133921/1‑148 (MQ=255)
                                                                                       actactacGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAAtt                                                             <  1:1588652/149‑1 (MQ=255)
                                                                                         tactacGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAAc                                                                          >  1:1434770/1‑134 (MQ=255)
                                                                                         tactacGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAAc                                                                          <  2:1434770/134‑1 (MQ=255)
                                                                                         tactacGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgc                                                           >  1:1066564/1‑149 (MQ=255)
                                                                                          actacGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgct                                                          <  1:1362976/149‑1 (MQ=255)
                                                                                            tacGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgctgc                                                        >  1:1153951/1‑149 (MQ=255)
                                                                                                ttGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTaa                                                    <  2:731278/149‑1 (MQ=255)
                                                                                                 tGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTTTCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAg                                                   >  2:1031630/1‑149 (MQ=255)
                                                                                                 tGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTTTCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAg                                                   >  2:1097915/1‑149 (MQ=255)
                                                                                                  gATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGc                                                  <  2:853559/149‑1 (MQ=255)
                                                                                                    tCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCa                                                <  1:591774/149‑1 (MQ=255)
                                                                                                       ttattCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAgcc                                             >  2:634286/1‑147 (MQ=255)
                                                                                                       ttattCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGAc                                             >  1:1569501/1‑149 (MQ=255)
                                                                                                       ttattCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGAc                                             >  1:10442/1‑149 (MQ=255)
                                                                                                       ttattCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCCGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGAc                                             <  2:82045/149‑1 (MQ=255)
                                                                                                          ttCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGc                                          >  2:1274591/1‑149 (MQ=255)
                                                                                                             tGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCTCTATCGTCTGCGACATATTGAGAAACAGGGTAATCGCTAGGCCAAACGCGTAGAAAAATTGCTGCGTAAGCCAGCCCGACTg                                       >  1:1024626/1‑149 (MQ=255)
                                                                                                             tGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTg                                       >  1:1018837/1‑149 (MQ=255)
                                                                                                             tGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACCACTCGAAAAATTGCTGCGTAAGCCAGACCGCCtt                                       >  1:986036/1‑148 (MQ=255)
                                                                                                               atcatcATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGAt                                     >  1:106696/1‑149 (MQ=255)
                                                                                                                   tgatGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGc                                 <  2:1383608/147‑1 (MQ=255)
                                                                                                                   tcatGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGTAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGc                                 <  1:687456/149‑1 (MQ=255)
                                                                                                                   tcatGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGc                                 >  2:1232872/1‑149 (MQ=255)
                                                                                                                   tcatGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGc                                 <  2:1466668/149‑1 (MQ=255)
                                                                                                                     ttggtggTGATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCt                               <  2:862041/148‑1 (MQ=255)
                                                                                                                      tggtggTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTg                              >  2:929898/1‑149 (MQ=255)
                                                                                                                      tggtggTCATTTCAGCCTATATTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTg                              <  2:1223723/149‑1 (MQ=255)
                                                                                                                        gtggtCATTTCAGCCTATTTTTCCGCGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGgt                            <  1:26562/149‑1 (MQ=255)
                                                                                                                           gtCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGa                                              >  1:598755/1‑128 (MQ=255)
                                                                                                                            tCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTa                        >  2:338166/1‑149 (MQ=255)
                                                                                                                              aattCAGCCTATTTTTCCGGGTCCGAAACCGGAATCATCACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAAAAGCTAGGACAAACGCGACGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGATAAt                      >  2:1389056/3‑149 (MQ=255)
                                                                                                                              aTTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAAt                      <  1:1213264/149‑1 (MQ=255)
                                                                                                                              aTTTCAGCCTATTTTTACGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGTGTAATCGCTCGGCCAAACGCGTCGAAATATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAAt                      <  2:262521/149‑1 (MQ=255)
                                                                                                                                ttCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCACCCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCg                    >  1:992355/1‑149 (MQ=255)
                                                                                                                                   aGCCTATTTTTCCGGGTCCGAAACCGGATTGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGATACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTACTCGGCa                 >  1:1167300/1‑149 (MQ=255)
                                                                                                                                    gCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCACCTGATAAGCCTGGTGTTAATCGGCaa                >  2:201095/1‑149 (MQ=255)
                                                                                                                                    gCCTATTTTTCCGCGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCaa                <  2:903680/149‑1 (MQ=255)
                                                                                                                                     ccTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGTCAAt               >  2:1509640/1‑149 (MQ=255)
                                                                                                                                      cTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATa              <  1:634320/149‑1 (MQ=255)
                                                                                                                                       tATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATaa             >  1:1456599/1‑149 (MQ=255)
                                                                                                                                       tATTTTTCCGGGTCCGAAACCGGAATGAAGACACTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAACCAGACCCCCTGATAAGCCTGGAGTTAATAGGCAATaa             >  2:698964/1‑149 (MQ=255)
                                                                                                                                       catcattCCGGTTTCGGACCCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGCCCGCCTGATAAGCCAGCTGTTAATCGGCAAGaa             >  1:1191624/6‑149 (MQ=255)
                                                                                                                                       catcattCCGGTTTCGGACCCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATaa             >  2:1107710/6‑149 (MQ=255)
                                                                                                                                       catcattCCGGTTTCGGACCCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTGAATCGGCAATaa             >  1:1185462/6‑149 (MQ=255)
                                                                                                                                        aTTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAAc            <  1:1445084/149‑1 (MQ=255)
                                                                                                                                        aTTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAAc            <  2:106696/149‑1 (MQ=255)
                                                                                                                                           tttCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGTTAATCGCTCGGCCAATCGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTg         <  2:872175/149‑1 (MQ=255)
                                                                                                                                           tttCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATacctca         >  1:735152/1‑144 (MQ=255)
                                                                                                                                           tttCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTg         <  1:1483981/149‑1 (MQ=255)
                                                                                                                                            ttCCGGTTTCGGACCCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTgg        >  2:538841/1‑149 (MQ=255)
                                                                                                                                             tCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGt       <  1:193585/149‑1 (MQ=255)
                                                                                                                                             tCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGt       <  2:1193945/149‑1 (MQ=255)
                                                                                                                                              ccGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTc      <  2:1182561/149‑1 (MQ=255)
                                                                                                                                                gggTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAACCGGCAATAACCTGGTCaa    <  2:686912/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCTGACACTGGCCTACTTCGGCAGCCGAATTGTCCAGCAGTTAATCAGCTAAACCCAGAAAAGGAGTTTCCCCTGGAACACATTTCTACTACTACGTTGATCATTATTCTGATCATCATGGTGGTCATTTCAGCCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATA  >  NC_000913/2748702‑2748996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑