Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,758,927 A→G 5.1% intergenic (+71/‑58) alpA → / → yfjI CP4‑57 prophage; DNA‑binding transcriptional activator/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,758,9270AG 5.1% 495.5 / 10.3 157intergenic (+71/‑58)alpA/yfjICP4‑57 prophage; DNA‑binding transcriptional activator/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (69/80);  new base G (5/3);  total (74/83)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 159 total aligned reads displayed.

ATGCTCGGCGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCT  >  NC_000913/2758779‑2759074
                                                                                                                                                    |                                                                                                                                                    
    tCGGCGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCgg                                                                                                                                                  <  2:15805/149‑1 (MQ=255)
      ggCGTGAAATCTGGCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGaa                                                                                                                                               <  1:85555/149‑1 (MQ=255)
       gCGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTCCGCTATTAATGGCGGaaat                                                                                                                                             <  1:990279/149‑3 (MQ=255)
         gTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAg                                                                                                                                            <  1:1017967/149‑1 (MQ=255)
         gTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAg                                                                                                                                            >  1:1348830/1‑149 (MQ=255)
          tGATATATGTCGGATGGATTGAGGCCGACATTGATGAGTGGTTATCACAACGCTGTAAACTTATATGAGGGTGTTACATGAATTAATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTATTAGCGGAATAGc                                                                                                                                           >  2:752664/1‑149 (MQ=255)
          tGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGc                                                                                                                                           <  2:973012/149‑1 (MQ=255)
           gAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTCCGCTATTAATGGCGGaaatagca                                                                                                                                         <  2:403764/149‑7 (MQ=255)
            aaaTCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCa                                                                                                                                         <  2:219750/149‑1 (MQ=255)
             aaTCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCat                                                                                                                                        <  1:1198428/149‑1 (MQ=255)
                      gatggatTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTaa                                                                                                                               >  2:572038/1‑149 (MQ=255)
                       atggatTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTaaa                                                                                                                              >  1:483159/1‑149 (MQ=255)
                          gatTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACaa                                                                                                                           >  1:1219739/1‑149 (MQ=255)
                          gatTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACaa                                                                                                                           >  1:1131204/1‑149 (MQ=255)
                              gAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTc                                                                                                                       >  2:819868/1‑149 (MQ=255)
                               aGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCt                                                                                                                      >  2:1372375/1‑149 (MQ=255)
                               aGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCt                                                                                                                      >  2:141553/1‑149 (MQ=255)
                                 gCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCtta                                                                                                                    >  2:128970/1‑149 (MQ=255)
                                  ccGAGATTGATGATTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaa                                                                                                                   <  1:746522/149‑1 (MQ=255)
                                  ccGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaa                                                                                                                   >  1:648820/1‑149 (MQ=255)
                                  ccGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTCTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaa                                                                                                                   <  2:340110/149‑1 (MQ=255)
                                   cGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaat                                                                                                                  >  1:1326428/1‑149 (MQ=255)
                                   cGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaat                                                                                                                  <  1:407711/149‑1 (MQ=255)
                                    gagaTTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaatt                                                                                                                 <  2:595072/149‑1 (MQ=255)
                                     agaTTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaattt                                                                                                                <  2:886785/149‑1 (MQ=255)
                                       tttGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGTGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaatttaa                                                                                                              <  2:302928/148‑1 (MQ=255)
                                        ttGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaatttaat                                                                                                             <  2:450191/149‑1 (MQ=255)
                                        ttGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCttaatttaat                                                                                                             <  1:540338/149‑1 (MQ=255)
                                          gatgaGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATttaatta                                                                                                           >  2:236438/1‑149 (MQ=255)
                                            tgaGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTaaa                                                                                                         <  1:1131629/149‑1 (MQ=255)
                                             taGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAAt                                                                                                        <  1:1421259/148‑1 (MQ=255)
                                             gaGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCTGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAAt                                                                                                        >  1:1005661/1‑149 (MQ=255)
                                             gaGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAAt                                                                                                        >  1:640298/1‑149 (MQ=255)
                                             gaGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATCTAATTAAAt                                                                                                        <  2:382916/149‑1 (MQ=255)
                                             gaGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCATGCTATTTCCGCCATAAATAGCGGAATAGCAATATTTTCTAAACAATTTCTTAATTTaaataaat                                                                                                        >  1:949749/1‑149 (MQ=255)
                                               gTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATaa                                                                                                      >  2:676215/1‑149 (MQ=255)
                                                 ggTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAaga                                                                                                    <  2:628821/149‑1 (MQ=255)
                                                 ggTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAaga                                                                                                    <  2:1403534/149‑1 (MQ=255)
                                                  gTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAagag                                                                                                   <  2:1163822/149‑1 (MQ=255)
                                                     aTCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGGTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCa                                                                                                >  1:406338/1‑149 (MQ=255)
                                                      tCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCAt                                                                                               <  2:1593971/149‑1 (MQ=255)
                                                        acaACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTAATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTg                                                                                             >  2:888592/1‑149 (MQ=255)
                                                            cGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATg                                                                                         >  2:513943/1‑149 (MQ=255)
                                                                 tAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCaa                                                                                    >  1:1501299/1‑149 (MQ=255)
                                                                 tAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCaa                                                                                    >  1:298696/1‑149 (MQ=255)
                                                                 tAAACTTATTTGAGGGTGTTACAAGAATTCATACTCAATTTCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCaa                                                                                    <  1:1199268/149‑1 (MQ=255)
                                                                  aaaCTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAAt                                                                                   <  2:741079/149‑1 (MQ=255)
                                                                   aaCTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATg                                                                                  >  2:577634/1‑149 (MQ=255)
                                                                   aaCTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATg                                                                                  <  2:92764/149‑1 (MQ=255)
                                                                     cTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGgt                                                                                >  1:1374446/1‑149 (MQ=255)
                                                                     cTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGgt                                                                                >  2:614356/1‑149 (MQ=255)
                                                                     cTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGgt                                                                                <  2:736660/149‑1 (MQ=255)
                                                                        aTTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAAAAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGttcgt                                                                             >  2:304826/1‑149 (MQ=255)
                                                                          ttGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTcc                                                                           >  1:188836/1‑149 (MQ=255)
                                                                            gAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCtt                                                                         >  2:233176/1‑149 (MQ=255)
                                                                             aGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCttt                                                                        <  2:1383125/149‑1 (MQ=255)
                                                                              gggTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTc                                                                       >  2:1048314/1‑149 (MQ=255)
                                                                               ggTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTcc                                                                      <  1:560801/149‑1 (MQ=255)
                                                                                gtgtTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATAttt                                                                                                                                    <  2:726107/86‑1 (MQ=255)
                                                                                gtgtTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATAttt                                                                                                                                    >  1:726107/1‑86 (MQ=255)
                                                                                  gtTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTg                                                                   <  2:483159/149‑1 (MQ=255)
                                                                                  gtTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTg                                                                   >  1:1250333/1‑149 (MQ=255)
                                                                                   ttACATGACTTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATATCCGCCATTAATAGCGGAATAGCCATTTTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTGCAATGGGCGGCCTTTCCCTGt                                                                  <  2:1591189/149‑1 (MQ=255)
                                                                                   ttACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGt                                                                  >  2:1409858/1‑149 (MQ=255)
                                                                                   ttACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGt                                                                  <  1:1372375/149‑1 (MQ=255)
                                                                                      cATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGa                                                               >  2:286110/1‑149 (MQ=255)
                                                                                       aTGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGAt                                                              >  2:522266/1‑149 (MQ=255)
                                                                                        tGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATg                                                             <  1:459739/149‑1 (MQ=255)
                                                                                              cATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTc                                                       <  1:982251/149‑1 (MQ=255)
                                                                                                tACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCt                                                     >  2:1464944/1‑149 (MQ=255)
                                                                                                    cAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTaaaa                                                 <  1:1258821/149‑1 (MQ=255)
                                                                                                     aaTTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATGTTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAAt                                                <  2:1251937/149‑1 (MQ=255)
                                                                                                     aaTTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCGTGTAGATGCAGTTCCTaaatt                                                >  2:790224/1‑147 (MQ=255)
                                                                                                         gCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATc                                            <  1:596064/149‑1 (MQ=255)
                                                                                                          cTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCa                                           <  2:1358795/149‑1 (MQ=255)
                                                                                                              cATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATATCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGaa                                       <  2:1327909/149‑1 (MQ=255)
                                                                                                                  ggAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCa                                   >  2:1353833/1‑149 (MQ=255)
                                                                                                                   gAGTGTTAACATCCTGCTATTTCCGCAATTAATATAGGAATAGGCATATTTGCTAAACAATTTCTTAATTTAATTTATTAAGAGGCATTTCATGTTCAATGGTCGTCCTTTCCCTGTAGATGGATTTCCTAAAATTATCAGGAATGCaa                                  <  2:1147902/149‑1 (MQ=255)
                                                                                                                    aGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAAt                                 >  1:823911/1‑149 (MQ=255)
                                                                                                                      tgtAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAAttt                               >  1:472012/1‑149 (MQ=255)
                                                                                                                      tgtAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAAttt                               >  2:1538344/1‑149 (MQ=255)
                                                                                                                        tAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTAt                             >  2:381031/1‑149 (MQ=255)
                                                                                                                           aCATCCTGCTATTTCCGCCATTAATAGCGGATTAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGaa                          >  1:135729/1‑149 (MQ=255)
                                                                                                                           aCATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGTCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGaa                          <  1:184743/149‑1 (MQ=255)
                                                                                                                           aCATCCTGCTATTTCCGACATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGaa                          <  1:236438/149‑1 (MQ=255)
                                                                                                                            cATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAg                         <  2:1501299/149‑1 (MQ=255)
                                                                                                                             aTCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGt                        <  1:233176/149‑1 (MQ=255)
                                                                                                                                atggctattCCGCTATTAATGGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGa                     >  1:144340/8‑149 (MQ=255)
                                                                                                                                 tggctattCCGCTATTAATGGCGGAAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGa                     >  2:391461/7‑149 (MQ=255)
                                                                                                                                 tGCTATTTCCGCCATTTATAGCGGAATAGCCATATTAGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATAAGGAATGCAATTAATGATGTGGaa                    >  2:1469688/1‑149 (MQ=255)
                                                                                                                                   gctattCCGCTATTAATGGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACa                  >  2:862167/5‑149 (MQ=255)
                                                                                                                                     tattCCGCTATTAATGGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGc                >  1:1494040/3‑149 (MQ=255)
                                                                                                                                           gCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGAATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGc          >  2:478988/1‑149 (MQ=255)
                                                                                                                                            ccATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGTTCGTCCTTTACCTGTAGATGCATTTCCTAAAATTATCAGGAATGAAATTTATGAAGTGCAACAGCATACGCa         >  1:814454/1‑149 (MQ=255)
                                                                                                                                            ccATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAAtt                                <  1:959759/126‑1 (MQ=255)
                                                                                                                                            ccATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAAtt                                >  2:959759/1‑126 (MQ=255)
                                                                                                                                            ccATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCa         <  2:188836/149‑1 (MQ=255)
                                                                                                                                             cATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAg        <  1:528627/149‑1 (MQ=255)
                                                                                                                                                  aTAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGcccc   >  1:564525/1‑149 (MQ=255)
                                                                                                                                                   tAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCt  <  1:1409858/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATGCTCGGCGTGAAATCTGTCGGATGGATTGAGGCCGAGATTGATGAGTGGTTATCACAACGCTGTAAACTTATTTGAGGGTGTTACATGAATTCATACTCAATTGCTGTCATCGGAGTGTAAACATCCTGCTATTTCCGCCATTAATAGCGGAATAGCCATATTTGCTAAACAATTTCTTAATTTAATTAAATAAGAGGCATTGCATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATTTATGAAGTGGAACAGCATACGCAGGCCCCT  >  NC_000913/2758779‑2759074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: