Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,804,528 C→A 9.4% intergenic (+67/‑287) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,804,5280CA 9.4% 261.5 / 3.2 105intergenic (+67/‑287)nrdF/proVribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (55/37);  new base A (2/8);  total (57/48)
Fisher's exact test for biased strand distribution p-value = 2.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGC  >  NC_000913/2804380‑2804676
                                                                                                                                                    |                                                                                                                                                      
tCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATc                                                                                                                                                        <  2:1079965/149‑1 (MQ=255)
     aTTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCa                                                                                                                                                   >  1:1369155/1‑149 (MQ=255)
      tttCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACTCaa                                                                                                                                                  >  2:853815/1‑149 (MQ=255)
          ttttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAaataa                                                                                                                                            >  1:342241/1‑149 (MQ=255)
           tttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAaataat                                                                                                                                           <  1:1449222/149‑1 (MQ=255)
           tttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAaataat                                                                                                                                           <  1:1428451/149‑1 (MQ=255)
            ttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAAtt                                                                                                                                          <  2:169307/149‑1 (MQ=255)
               cGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtgt                                                                                                                                       <  1:1046509/149‑1 (MQ=255)
                tgTTCAGGCTCCTCTTATGTGATGGTGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATATTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtgtg                                                                                                                                      <  1:1394863/148‑1 (MQ=255)
                 gTTCAGGCTCCTCTTATGTAATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtgg                                                                                                                                     <  1:1383709/149‑1 (MQ=255)
                     aGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAt                                                                                                                                 <  1:1202521/149‑1 (MQ=255)
                     aGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAt                                                                                                                                 <  1:959319/149‑1 (MQ=255)
                     aGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAt                                                                                                                                 <  2:882103/149‑1 (MQ=255)
                     aGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACACGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTATATCACGCAAATAATTAGTGGTGAt                                                                                                                                 >  1:1043289/1‑149 (MQ=255)
                       gCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCt                                                                                                                               <  1:1205315/149‑1 (MQ=255)
                         tcctcTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGTTGATCTac                                                                                                                             >  1:1559530/1‑149 (MQ=255)
                         tcctcTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTac                                                                                                                             >  1:1262929/1‑149 (MQ=255)
                           ctctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGTGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTacac                                                                                                                           <  1:798273/149‑1 (MQ=255)
                           ctctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTacac                                                                                                                           <  1:1097213/149‑1 (MQ=255)
                            tctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACt                                                                                                                          >  1:869195/1‑149 (MQ=255)
                              ttATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGa                                                                                                                        >  1:1057297/1‑149 (MQ=255)
                               tATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATAACGCAAATAATTTGTGGTGATCTACACTGAt                                                                                                                       >  2:224172/1‑149 (MQ=255)
                                aTGTGTTGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGTGAAATAATTCCCGCCAAATAGCTTTTTATCCCGCAAATAATTTGTGGTGATCTACACTGATa                                                                                                                      <  1:886109/149‑1 (MQ=255)
                                  gtgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCTCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATAct                                                                                                                    >  2:557280/1‑149 (MQ=255)
                                  gtgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCAGCCAATTTTTTTTTTTTATGTTAATGATTTTTTTGTGATCTACACTGATAct                                                                                                                    <  2:150965/149‑1 (MQ=255)
                                    gATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATActct                                                                                                                  >  2:1507647/1‑149 (MQ=255)
                                    gATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTACTGAGAGTGATATTTTAAAAAATATCACAACCCGCAGCAGGCAAAAAATAACAGCAAAATAGCATTTTATAAAGCAAATAAATTGAGTTGATCTACACTGATActct                                                                                                                  >  1:1014374/1‑149 (MQ=255)
                                     aTGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATActctt                                                                                                                 >  2:165968/1‑148 (MQ=255)
                                     aTGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTg                                                                                                                 >  2:903161/1‑149 (MQ=255)
                                     aTGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTg                                                                                                                 <  1:500244/149‑1 (MQ=255)
                                       ggggAAAGCTGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGTGAAATAATTCCCGCCAAATAGCTTTTAATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGtt                                                                                                               <  1:1291421/149‑1 (MQ=255)
                                       ggggAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGtt                                                                                                               <  1:1545483/149‑1 (MQ=255)
                                        gtgAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTg                                                                                                              >  2:1036676/3‑149 (MQ=255)
                                        gggAAAGCGGTTGAAACAGAATATGAAGTCTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACTCAAATAATTTGTGGTGATCTACACTGATACTCTGTTg                                                                                                              <  1:1393719/149‑1 (MQ=255)
                                         ggAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCAAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATATACACTGATACTCTGTTga                                                                                                             >  1:665366/1‑148 (MQ=255)
                                         ggAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACACGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGc                                                                                                             >  1:1315530/1‑149 (MQ=255)
                                          gAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCa                                                                                                            <  1:1229979/149‑1 (MQ=255)
                                          gAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCa                                                                                                            >  1:1462740/1‑149 (MQ=255)
                                                ggTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCattatta                                                                                                      >  2:1220100/1‑148 (MQ=255)
                                                      aaCAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGa                                                                                                >  2:742748/1‑149 (MQ=255)
                                                      aaCAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCAGCCAAATAGCTTTTAAACACGCAAAAAATATGTGGTGAACTACACTGATACTATTTTGCATAATTAGCATGa                                                                                                >  1:283623/1‑149 (MQ=255)
                                                        ctgacgaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCATCATTGAAATAATTCCCGTCAAATTTTTTTTTTTATGTTAATGAGATTTGGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGaaa                                                                                              <  2:258802/144‑1 (MQ=255)
                                                        cAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGaaa                                                                                              <  2:1305666/149‑1 (MQ=255)
                                                        cAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGaaa                                                                                              >  2:138029/1‑149 (MQ=255)
                                                         agaagaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAAc                                                                                             >  2:1369776/1‑149 (MQ=255)
                                                          gaagaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAAcc                                                                                            >  2:516624/1‑149 (MQ=255)
                                                              aTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACaa                                                                                        <  1:464857/149‑1 (MQ=255)
                                                                taaGACTTGAATTTCTGAGTGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGACAAATATTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAata                                                                                      <  2:1303940/148‑1 (MQ=255)
                                                                gAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAata                                                                                      <  1:742748/149‑1 (MQ=255)
                                                                gAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAata                                                                                      >  1:814808/1‑149 (MQ=255)
                                                                      tGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCATATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAgg                                                                                >  1:97592/1‑149 (MQ=255)
                                                                      tGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAgg                                                                                >  1:1422506/1‑149 (MQ=255)
                                                                      tGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAgg                                                                                >  2:1307107/1‑149 (MQ=255)
                                                                      tGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAgg                                                                                >  1:741561/1‑149 (MQ=255)
                                                                       ggAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGc                                                                               >  1:156172/1‑149 (MQ=255)
                                                                        gAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTTCATTATTCGCCTGAAACCACAAAATTCATGCg                                                                              >  2:149260/1‑149 (MQ=255)
                                                                        gAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCg                                                                              >  1:1480472/1‑149 (MQ=255)
                                                                        gAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCAGTTGCATTATTCGCCTGAAACAACAATATTCAGGCg                                                                              >  2:59248/1‑149 (MQ=255)
                                                                        gAATTTCTGAGGGTGTTATATTCAAAAATATCACTACCAGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCAACACTCATACTCTGTAGCATTATTCGCCTGAAACCACAAAATTCAGGCg                                                                              >  1:1075334/1‑149 (MQ=255)
                                                                           tttCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAAACACAATATTCAGGCGttt                                                                           >  2:775580/1‑149 (MQ=255)
                                                                           tttATGAGTGTTTGATTTTCAACTATATCACTAACAGCATCATTGAAATAAATCCAGCCAAATAGATTTTTATCACGCAAATAATTTGTTGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTAAGGCGttt                                                                           <  1:794852/149‑1 (MQ=255)
                                                                                gAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCTCGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCg                                                                      <  2:1246085/149‑1 (MQ=255)
                                                                                   ggTGTTATTTTCAAAAATATCACTACCCGCAGCAGGAAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTa                                                                   >  1:948930/1‑149 (MQ=255)
                                                                                       ttATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCtt                                                               >  2:679259/1‑149 (MQ=255)
                                                                                            ttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATATATTTTTATCTCTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACa                                                          <  2:1267001/149‑1 (MQ=255)
                                                                                            ttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACa                                                          >  2:704144/1‑149 (MQ=255)
                                                                                            ttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTCATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACa                                                          >  2:1519925/1‑149 (MQ=255)
                                                                                                       cACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCaa                                               >  1:484800/1‑149 (MQ=255)
                                                                                                        aCTACCCGCAGCAGTGAAATAATTCCCGACAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGTCGTATTTTCGCTATCTTTGACAAAAAATATCAAc                                              >  2:1243609/1‑149 (MQ=255)
                                                                                                          tACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAatat                                                  >  2:1407579/1‑143 (MQ=255)
                                                                                                          tACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAatat                                                  <  1:1407579/143‑1 (MQ=255)
                                                                                                           aCCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACttt                                           <  1:516624/149‑1 (MQ=255)
                                                                                                                    agGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCttt                                                              <  1:177331/121‑1 (MQ=255)
                                                                                                                    agGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCttt                                                              >  2:177331/1‑121 (MQ=255)
                                                                                                                    agGGAAATAATTACCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTATGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCTATATATTTTACAAAAAATATCAAATTTCTCGAtttt                                  >  2:408398/1‑148 (MQ=255)
                                                                                                                      ggAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTTGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTGGATTTGct                                <  2:661365/149‑1 (MQ=255)
                                                                                                                      ggAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGct                                >  2:877433/1‑149 (MQ=255)
                                                                                                                       gAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTActc                               >  2:401401/1‑149 (MQ=255)
                                                                                                                        aaaTAATTCCCGCCAATTTTTTTTTTTTAAGTAATTTATTTTGTTGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGGGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGctct                              <  2:1075334/149‑1 (MQ=255)
                                                                                                                        aaaTAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGctct                              <  1:1117104/149‑1 (MQ=255)
                                                                                                                         aataatTCCCGCAAATTAGTTTTTTATCACGCAAATAATTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCtctc                             <  2:283623/149‑1 (MQ=255)
                                                                                                                             atTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGcc                         <  1:165968/149‑1 (MQ=255)
                                                                                                                              ttCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGccc                        >  1:353663/1‑149 (MQ=255)
                                                                                                                               tCCCGCCAAATAGATTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCt                       <  2:736455/149‑1 (MQ=255)
                                                                                                                                 ccGCCAAATAGCTTTTTATCAAGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTta                     <  1:927083/149‑1 (MQ=255)
                                                                                                                                  cGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTtat                    >  2:1401982/1‑149 (MQ=255)
                                                                                                                                    ccAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTatat                  >  2:1051340/1‑149 (MQ=255)
                                                                                                                                    ccAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTatat                  >  2:1482228/1‑149 (MQ=255)
                                                                                                                                    ccAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCCCAGCCCTTatat                  >  1:385952/1‑149 (MQ=255)
                                                                                                                                       aaTAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGTTACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCAc               >  2:237883/1‑149 (MQ=255)
                                                                                                                                       aaTAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCtctc                             >  1:450491/1‑135 (MQ=255)
                                                                                                                                       aaTAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCtctc                             <  2:450491/135‑1 (MQ=255)
                                                                                                                                        attgCTTTTTATAAGGTAAATATTTTTTGGTTATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACg              <  2:1315530/146‑1 (MQ=255)
                                                                                                                                        atatCTTTTTATAAAGCAAATAATTTGTGGTTATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACATTATTCACGCGTTTTTTCGCTATCTTTGACAAATAATATCATCTTTCTCTATTTGCTCTCAGCCCTTATATCTCg              <  1:470570/149‑1 (MQ=255)
                                                                                                                                        aTAGCTTTTTATCACGCAAATATTTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACg              <  2:1262929/149‑1 (MQ=255)
                                                                                                                                        aTAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACg              <  1:1369776/149‑1 (MQ=255)
                                                                                                                                             tttttATCAGTCAATTAATTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGaaa         <  2:869195/149‑1 (MQ=255)
                                                                                                                                                 tATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGATTTTTCGCTATCTATGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTcc     >  1:239661/1‑149 (MQ=255)
                                                                                                                                                  aTCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCg    >  1:572390/1‑149 (MQ=255)
                                                                                                                                                    cACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCATGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGc  <  1:319213/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TCACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGC  >  NC_000913/2804380‑2804676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: