Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,874,306 | G→A | 6.6% | R372C (CGC→TGC) | cysN ← | sulfate adenylyltransferase, subunit 1 |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,874,306 | 0 | G | A | 6.6% | 185.9 / 2.9 | 60 | R372C (CGC→TGC) | cysN | sulfate adenylyltransferase, subunit 1 |
Reads supporting (aligned to +/- strand): ref base G (27/29); new base A (2/2); total (29/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 |
TCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCTGCCGCCAGCAGCAG > NC_000913/2874165‑2874453 | tCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCa < 2:116659/149‑1 (MQ=255) cAGCCCACCCGTCACCGGATTTTGTTGCTAACGATCTTACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGGGGCAGGTTTTCAACTTCACGATGGGTAAGGTTATTAATATCAACCTGATAGCGATTGCCAt < 2:788838/149‑1 (MQ=255) aGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATc < 1:1093893/149‑1 (MQ=255) aGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATc < 1:1340631/149‑1 (MQ=255) aGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGACATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATc < 2:1223074/149‑1 (MQ=255) aCCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAacac > 2:1598868/1‑149 (MQ=255) aCCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAacac > 2:519635/1‑149 (MQ=255) gTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACAcgcgc > 1:54912/1‑149 (MQ=255) ggATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCTCGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGt < 2:195798/149‑1 (MQ=255) ggATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGCTCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCACCCTGATAGCGAATGCCATCACCCCGCGCGCGCGt > 2:287114/1‑149 (MQ=255) gATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGtc > 2:125040/1‑149 (MQ=255) gATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGtc < 2:1433139/149‑1 (MQ=255) gttgATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTa < 2:149307/149‑1 (MQ=255) ttgATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTAc < 2:590735/149‑1 (MQ=255) tgATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTAcc < 1:1486866/149‑1 (MQ=255) tAACGATCTACCACCAGCGGCTCGTCAACAGTGAGATCCTCGAGGACGATCCCATTCAGTGGCAGGTTTTCAACTTCCCGCTGGGTAAGGTTATTAATATCACCCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGc > 1:242612/1‑149 (MQ=255) tCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAAtttt < 2:1315384/149‑1 (MQ=255) tAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGa < 1:1320353/149‑1 (MQ=255) tAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGa < 1:1371795/149‑1 (MQ=255) aaCACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGACGATCCCATTCAGTGGCAGGTTTTCAACTTAAAGCTGCGTAAGGTTATAAATATAAAACTGATAGCGAATGCCATCAACACGCGCGCGCATCTTCTTACCGGCaaaattcat > 1:1003456/1‑142 (MQ=255) cAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGt > 2:545601/1‑149 (MQ=255) cTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGACAATTTTGATGTCGTAActct < 1:1037499/149‑1 (MQ=255) gTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGcc < 2:1286737/149‑1 (MQ=255) gTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGcc > 1:136196/1‑149 (MQ=255) gTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCAAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGcc > 1:1391815/1‑149 (MQ=255) gTCAAAAGTGAGATCCAAGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGcc > 1:218654/1‑149 (MQ=255) aaaaGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTg > 2:1542971/1‑149 (MQ=255) aaGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGga < 2:1473547/149‑1 (MQ=255) aaGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGga < 1:545601/149‑1 (MQ=255) aaGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGga > 2:751133/1‑149 (MQ=255) tGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTAGCGGCATTTTTGATGTCGTACCTCTGCCCTGGAGaa > 1:275087/1‑149 (MQ=255) ccGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCAAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGc < 2:1391815/149‑1 (MQ=255) aTCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAATGTTATTAATATAAAACTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTATTTCCGGCAATTTTGATGTCGGAACTCTGCCCTGGAGAACGCGGCGGTTCCCccaa > 2:791257/1‑148 (MQ=255) aatCAGTGGCAGGTTATCAAATTCACGCTGGGAAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACAGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATa < 1:553907/147‑1 (MQ=255) aTTCAGTGGCAGGTTTTCAACTTCACTCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGGCTACTTACCGGCTATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCTGCTGTTCCGCCATCCATa > 2:983244/1‑149 (MQ=255) aTTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATa > 1:1097655/1‑149 (MQ=255) aTTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCAAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATa > 2:1357848/1‑149 (MQ=255) ttCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATAc > 2:1085447/1‑149 (MQ=255) ttCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATAc < 2:653076/149‑1 (MQ=255) ttCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATAc < 2:511137/149‑1 (MQ=255) cAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACca < 1:1357343/149‑1 (MQ=255) cAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATATTGATGTCGTAAATCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCa > 2:1424397/1‑149 (MQ=255) tttCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGAc < 1:519635/149‑1 (MQ=255) tttCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGAc < 1:1598868/149‑1 (MQ=255) cACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGc < 1:1539710/149‑1 (MQ=255) aCGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCa > 1:1172773/1‑149 (MQ=255) aGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTaa > 2:1151385/1‑149 (MQ=255) aGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTaa > 2:234562/1‑149 (MQ=255) ggTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAAc < 1:1085447/149‑1 (MQ=255) gTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACg > 2:942175/1‑149 (MQ=255) ttattaATATCAACCTGATAGCGACTGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGc < 1:287114/149‑1 (MQ=255) tattaATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCt < 1:125040/149‑1 (MQ=255) ttaATATCAACCTGATAGCAAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTc < 1:1357848/149‑1 (MQ=255) tatCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCt < 1:608541/149‑1 (MQ=255) atCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCTg > 1:1246258/1‑149 (MQ=255) cAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCTgcc > 1:820166/1‑149 (MQ=255) aCCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCTgccgc > 1:1435644/1‑149 (MQ=255) tAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCTGCCGCcagcag < 1:751133/149‑1 (MQ=255) cGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCCCCGCCGGTAACGCTTCGTCTGCCGCcagcagcag > 1:236057/1‑149 (MQ=255) cGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCTGCCGCcagcagcag > 2:1524912/1‑149 (MQ=255) | TCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTAATATCAACCTGATAGCGAATGCCATCAACACGCGCGCGCGTCTTCTTACCGGCAATTTTGATGTCGTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCTGCACCGCCGGTAACGCTTCGTCTGCCGCCAGCAGCAG > NC_000913/2874165‑2874453 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |