Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,894,852 T→G 8.8% intergenic (+81/+67) ygcP → / ← ygcQ putative antiterminator regulatory protein/putative flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,894,8520TG 8.8% 225.3 / 12.7 80intergenic (+81/+67)ygcP/ygcQputative antiterminator regulatory protein/putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (29/44);  new base G (3/4);  total (32/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

ACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGC  >  NC_000913/2894704‑2894996
                                                                                                                                                    |                                                                                                                                                
aCGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCt                                                                                                                                                  >  2:230089/1‑149 (MQ=255)
      gtgtCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCCGCGCCTGATGCGACGCTTGAcgc                                                                                                                                            >  2:1209032/1‑149 (MQ=255)
         tCGTGTCGCTTTCCACCAAGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc                                                                                                                                         <  2:1168509/149‑1 (MQ=255)
         tCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc                                                                                                                                         <  2:655174/149‑1 (MQ=255)
          cGTGGCGCTTTCCACCACGTATTCCGGGGTCTGGACGTTAGAGATAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCt                                                                                                                                        <  1:54676/149‑1 (MQ=255)
             ggCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                                                                     <  1:705537/149‑1 (MQ=255)
             ggCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                                                                     <  2:488633/149‑1 (MQ=255)
              gcgcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATCGAATTTGGTTTGATTTGTAGGCAGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                                                                    >  2:605893/1‑149 (MQ=255)
                gcTTTCCACCACGAATACCGGGGTCTGGACGTTATCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                  <  2:1324923/149‑1 (MQ=255)
                gcTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                  <  1:1263707/149‑1 (MQ=255)
                 cTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATACTCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAgg                                                                                                                                 <  1:822586/149‑1 (MQ=255)
                  tttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATACGAAATTCAGCGCCTGATGCGACCATTGCCGCGTCTTATCCGGc                                                                                                                                >  2:258277/1‑149 (MQ=255)
                  tttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACGTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCAACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGc                                                                                                                                >  1:247068/1‑149 (MQ=255)
                   ttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcg                                                                                                                               <  1:83477/149‑2 (MQ=255)
                   ttCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATAAGACGCGTCAAGCGTCGCATCAGGcg                                                                                                                               <  2:1341837/149‑2 (MQ=255)
                       accacGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACa                                                                                                                           <  2:397705/149‑1 (MQ=255)
                                 ccGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg                                                                                                                 >  1:1389057/1‑149 (MQ=255)
                                 ccGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAgg                                                                                                                 <  2:942678/149‑1 (MQ=255)
                                  gggggTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAAGCACTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCTGGCCTACAAGTCCCCAGGc                                                                                                                <  2:1576593/148‑1 (MQ=255)
                                  cGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc                                                                                                                <  2:47722/149‑1 (MQ=255)
                                  cGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc                                                                                                                <  2:1499682/149‑1 (MQ=255)
                                     ggTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAg                                                                                                             <  2:755425/149‑1 (MQ=255)
                                       tCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGaa                                                                                                           <  2:729478/149‑1 (MQ=255)
                                        cTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAAc                                                                                                          <  1:457677/149‑1 (MQ=255)
                                           gACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGACTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGt                                                                                                       >  2:1170242/1‑149 (MQ=255)
                                            aCGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTa                                                                                                      <  1:327456/149‑1 (MQ=255)
                                             cGTTCGCGAAAAAATTACTTTGACGGGATAATCGTATCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAg                                                                                                     <  1:974041/149‑1 (MQ=255)
                                                tAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa                                                                                                  >  2:1540732/1‑149 (MQ=255)
                                                tAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa                                                                                                  >  1:1076307/1‑149 (MQ=255)
                                                tAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGACGCACGACAAATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTATTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCa                                                                                                  >  1:287346/1‑149 (MQ=255)
                                                 aGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAg                                                                                                 <  2:1349389/149‑1 (MQ=255)
                                                        aaaTTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcatc                                                                                          <  1:982560/149‑1 (MQ=255)
                                                        aaaTTACTTTGACGGGATAATCGTAACCAATTGAATTTGGATTGATTTGTAGGCCGCACGCCACATACGACATACAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcatc                                                                                          >  2:640816/1‑149 (MQ=255)
                                                            tACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGc                                                                                      <  1:230089/149‑1 (MQ=255)
                                                               tttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                   >  2:52047/1‑149 (MQ=255)
                                                               tttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                   >  2:1427781/1‑149 (MQ=255)
                                                               tttGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGAAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAac                                                                                   >  2:6847/1‑149 (MQ=255)
                                                                       gATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTAtt                                                                           <  2:212204/149‑1 (MQ=255)
                                                                        aTAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTg                                                                          <  2:678428/149‑1 (MQ=255)
                                                                        aTAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACATTATTg                                                                          <  1:553564/149‑1 (MQ=255)
                                                                         tAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACAGTAGGCAGGATCATCCGGCAACACGTTATTgg                                                                         >  1:1098419/1‑149 (MQ=255)
                                                                          aaTCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGc                                                                        >  2:952641/1‑149 (MQ=255)
                                                                            tCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGAGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACAAGTTATTGGCaa                                                                      >  1:1138092/1‑149 (MQ=255)
                                                                             cGTAACCAATTGAATTTGGTTTGAGTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAAt                                                                     <  2:267320/149‑1 (MQ=255)
                                                                               tAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCATAGGCAGGATCATCCGGCAACACGTTATTGGCAATCt                                                                   >  1:739273/1‑149 (MQ=255)
                                                                                  ccAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCg                                                                <  2:247068/149‑1 (MQ=255)
                                                                                  ccAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCg                                                                >  1:1315451/1‑149 (MQ=255)
                                                                                   cAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGt                                                               <  1:1335449/149‑1 (MQ=255)
                                                                                    aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACTCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                              >  2:1416982/1‑149 (MQ=255)
                                                                                    aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                              >  2:561762/1‑149 (MQ=255)
                                                                                    aaTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTg                                                              >  1:632496/1‑149 (MQ=255)
                                                                                       tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGAGATTAAGCGCCTGATGCGACGCTTGAAGCGTCTTATCAGGCCTACAAGTCACCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                           <  1:258277/149‑1 (MQ=255)
                                                                                       tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                           <  2:1117652/149‑1 (MQ=255)
                                                                                       tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                           <  2:1111966/149‑1 (MQ=255)
                                                                                       tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCATAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAAt                                                           <  2:739273/149‑1 (MQ=255)
                                                                                        gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATg                                                          >  1:959179/1‑149 (MQ=255)
                                                                                           tttGGTTTGATTTGTAGGCCGCCCGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                       <  1:1224858/149‑1 (MQ=255)
                                                                                           tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                       <  1:429327/149‑1 (MQ=255)
                                                                                           tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                       <  1:561762/149‑1 (MQ=255)
                                                                                           tttGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGttt                                                       <  2:737363/149‑1 (MQ=255)
                                                                                              ggTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc                                                    <  1:1209032/149‑1 (MQ=255)
                                                                                                  tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCa                                                <  1:1393105/149‑1 (MQ=255)
                                                                                                    aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg                                                                                                    <  2:1543062/95‑1 (MQ=255)
                                                                                                    aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg                                                                                                    >  1:1543062/1‑95 (MQ=255)
                                                                                                    aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCTATCTGCGTGAATGTTTGTCACCAGt                                              >  1:795494/1‑149 (MQ=255)
                                                                                                            gCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAg                                      <  1:493489/149‑1 (MQ=255)
                                                                                                              cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGca                                    >  1:921490/1‑149 (MQ=255)
                                                                                                              cGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGca                                    >  1:159232/1‑149 (MQ=255)
                                                                                                               gCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGcac                                   <  1:48226/149‑1 (MQ=255)
                                                                                                                         tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAg                         >  1:1079845/1‑149 (MQ=255)
                                                                                                                           cGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtc                       <  1:952641/149‑1 (MQ=255)
                                                                                                                            gACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtca                      <  1:868495/149‑1 (MQ=255)
                                                                                                                              cATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtcatc                    <  1:52047/149‑1 (MQ=255)
                                                                                                                                  cAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAAcc                <  2:1287148/149‑1 (MQ=255)
                                                                                                                                      gcCTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGc            >  1:48091/1‑149 (MQ=255)
                                                                                                                                        cTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGACCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCa          >  2:1424110/1‑149 (MQ=255)
                                                                                                                                        cTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCaa                  >  2:1415992/1‑141 (MQ=255)
                                                                                                                                        cTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCaa                  <  1:1415992/141‑1 (MQ=255)
                                                                                                                                          gataagACGCGTCAAGCGTCGCATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAAc        >  1:920832/6‑149 (MQ=255)
                                                                                                                                                ccGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCTATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGc  <  2:258113/148‑1 (MQ=255)
                                                                                                                                                aCGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGc  >  2:959185/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ACGCGGGTGTCGTGGCGCTTTCCACCACGAATACCGGGGTCTGGACGTTAGCGAAAAAATTACTTTGACGGGATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAACCACGCCAACATCTGC  >  NC_000913/2894704‑2894996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: